##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926823_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6472 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.696693448702103 28.0 16.0 31.0 2.0 33.0 2 24.514833127317676 28.0 23.0 31.0 2.0 33.0 3 24.82725587144623 29.0 25.0 31.0 2.0 34.0 4 28.127781211372064 33.0 27.0 35.0 2.0 37.0 5 27.21801606922126 33.0 25.0 35.0 2.0 37.0 6 26.79264524103832 33.0 22.0 35.0 2.0 37.0 7 26.49613720642769 33.0 19.0 35.0 2.0 37.0 8 26.2909456118665 33.0 17.0 35.0 2.0 37.0 9 27.146013597033374 34.0 17.0 38.0 2.0 39.0 10 26.966934487021014 34.0 17.0 38.0 2.0 39.0 11 26.95673671199011 34.0 17.0 38.0 2.0 39.0 12 26.722033374536466 34.0 17.0 38.0 2.0 39.0 13 26.657292954264523 34.0 15.0 38.0 2.0 39.0 14 27.06597651421508 34.0 16.0 39.0 2.0 41.0 15 26.96229913473424 34.0 11.0 39.0 2.0 41.0 16 26.909919653893695 34.0 10.0 39.0 2.0 41.0 17 26.898331273176762 34.0 10.0 39.0 2.0 41.0 18 26.70720024721879 34.0 10.0 39.0 2.0 41.0 19 26.73423980222497 34.0 8.0 39.0 2.0 41.0 20 26.616501854140914 34.0 2.0 39.0 2.0 41.0 21 26.537546353522867 34.0 2.0 39.0 2.0 41.0 22 26.338226205191596 34.0 2.0 39.0 2.0 41.0 23 26.126081582200246 34.0 2.0 39.0 2.0 41.0 24 26.04836217552534 34.0 2.0 39.0 2.0 41.0 25 25.81906674907293 34.0 2.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.0197196900322005 1101 2 -0.34065426760738404 1101 3 0.3221582237207272 1101 4 -1.4483740218115315 1101 5 0.4155862366799923 1101 6 -4.531050763472642 1101 7 -2.331129981129976 1101 8 -0.696585445804196 1101 9 -3.21518204018205 1101 10 -2.5867283324314556 1101 11 -0.6817403950216452 1101 12 -1.8686897304084837 1101 13 -1.8837044899545 1101 14 -4.057907760642131 1101 15 -5.081646261724387 1101 16 -2.6227489090770284 1101 17 -3.3705733502608552 1101 18 -1.289386308136308 1101 19 -0.08955194111443276 1101 20 -0.7954927884615302 1101 21 -1.5565520850677004 1101 22 -0.5312547261766092 1101 23 -1.0862941745754355 1101 24 -1.0631415025946325 1101 25 0.43122316052004095 1102 1 1.3439166736041663 1102 2 1.3866184596653426 1102 3 -0.7687508671883663 1102 4 0.642535069097562 1102 5 1.3246771457709023 1102 6 2.2871310547091745 1102 7 2.5779609279609303 1102 8 0.7579600087412572 1102 9 3.3302725052724966 1102 10 3.3223625766594544 1102 11 3.8637141504329016 1102 12 3.2222193605006098 1102 13 3.479931873681867 1102 14 3.3966376939033225 1102 15 4.009262829184703 1102 16 3.0136147272866083 1102 17 4.174881195193688 1102 18 3.9833409645909654 1102 19 2.183175331612837 1102 20 2.11359812062938 1102 21 1.9889024603868464 1102 22 1.5596543647324808 1102 23 2.095524007242748 1102 24 1.755040315587184 1102 25 1.431223160520041 1103 1 1.162098491785983 1103 2 1.5886386616855432 1103 3 -0.3041044025418991 1103 4 -0.5998891733266802 1103 5 -1.7359289148351564 1103 6 2.8628886304667525 1103 7 -0.5735542235542184 1103 8 1.869071119852368 1103 9 0.1383533133533028 1103 10 -5.394809140512262 1103 11 -0.38881110209235104 1103 12 0.9797951180763675 1103 13 0.40922480297479424 1103 14 -2.1892208919552623 1103 15 1.5042123241341976 1103 16 -1.8954761818043018 1103 17 -1.0271390068265127 1103 18 1.3469773282273287 1103 19 1.8094379578754634 1103 20 2.4570324640637224 1103 21 -0.9201884487040637 1103 22 -0.036305231227114376 1103 23 -1.005486093767356 1103 24 -0.7803132197663487 1103 25 1.4615261908230721 1104 1 0.745431825119315 1104 2 -1.0224724494255675 1104 3 -0.27632662476412406 1104 4 0.06677749333998406 1104 5 -0.15259558150182428 1104 6 -1.1371113695332475 1104 7 1.843112443112446 1104 8 1.3412933420745894 1104 9 1.527242202242192 1104 10 3.549635303932181 1104 11 2.416744453463206 1104 12 0.8131284514096997 1104 13 0.4647803585303514 1104 14 2.783001330266959 1104 15 -1.1346765647546917 1104 16 3.9378571515290304 1104 17 2.66730543761793 1104 18 -0.2363560051060034 1104 19 1.1983268467643526 1104 20 1.484810241841501 1104 21 0.9131448846292685 1104 22 2.5748058798839963 1104 23 0.5500694617882012 1104 24 1.1641312246780942 1104 25 0.5448595241564043 1105 1 -3.383356053668564 1105 2 -4.5224724494255675 1105 3 -2.7687508671883663 1105 4 -3.7211012945388013 1105 5 -1.6753228542290977 1105 6 -1.0765053089271888 1105 7 -0.33112998112997616 1105 8 -1.0602218094405593 1105 9 0.6939088689088599 1105 10 -0.4049101506132722 1105 11 -0.49992221320346175 1105 12 0.22221936050060975 1105 13 -0.520068126318133 1105 14 0.9420922393578692 1105 15 1.1001719200937927 1105 16 -1.4409307272588485 1105 17 -0.0069369866244919365 1105 18 1.9833409645909654 1105 19 1.0013571497946572 1105 20 -0.06822006118880353 1105 21 2.5343570058413896 1105 22 -0.7130729079947891 1105 23 -3.449930538211799 1105 24 -1.6995051389582692 1105 25 -0.659685930389049 1106 1 4.050987380674872 1106 2 2.8108608839077647 1106 3 3.2514511530136545 1106 4 2.622333048895541 1106 5 4.264071085164844 1106 6 3.7517775193556417 1106 7 5.648667998668003 1106 8 6.091293342074593 1106 9 4.805019980019974 1106 10 7.605190859487738 1106 11 5.3889666756854275 1106 12 5.202017340298589 1106 13 6.409224802974798 1106 14 0.3663346636002913 1106 15 0.17087899080086544 1106 16 1.4378571515290304 1106 17 4.861749882062377 1106 18 0.45808843933843946 1106 19 3.142771291208799 1106 20 5.345921352952612 1106 21 6.524255995740379 1106 22 3.9636947687728856 1106 23 0.5500694617882012 1106 24 2.2196867802336513 1106 25 2.239303968600847 1107 1 1.3620984917859822 1107 2 3.077527550574434 1107 3 3.140340041902544 1107 4 4.833444160006653 1107 5 6.897404418498173 1107 6 6.696221963800085 1107 7 4.659779109779112 1107 8 7.557960008741254 1107 9 6.5939088689088585 1107 10 4.849635303932182 1107 11 6.60007778679654 1107 12 7.213128451409698 1107 13 9.59811369186368 1107 14 6.933001330266958 1107 15 6.381990101911978 1107 16 5.404523818195695 1107 17 8.1839721042846 1107 18 7.346977328227329 1107 19 7.464993513431022 1107 20 6.168143575174835 1107 21 7.579811551295936 1107 22 7.741472546550664 1107 23 8.4500694617882 1107 24 4.3641312246780934 1107 25 8.794859524156404 1108 1 7.384320714008204 1108 2 4.699749772796654 1108 3 6.695895597458101 1108 4 6.5112219377844305 1108 5 8.152959974053733 1108 6 8.196221963800085 1108 7 7.870890220890228 1108 8 7.646848897630143 1108 9 8.249464424464417 1108 10 10.716301970598845 1108 11 6.1667444534632025 1108 12 10.090906229187478 1108 13 9.964780358530348 1108 14 8.810779108044738 1108 15 11.39310121302309 1108 16 10.771190484862363 1108 17 9.639527659840155 1108 18 11.346977328227329 1108 19 11.920549068986578 1108 20 8.901476908508169 1108 21 11.635367106851493 1108 22 12.852583657661778 1108 23 12.32784723956598 1108 24 12.219686780233651 1108 25 11.239303968600847 1109 1 -3.9379015082140185 1109 2 -1.8224724494255682 1109 3 -2.9596599580974576 1109 4 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-4.812997419247427 2213 14 -6.411443114177484 2213 15 -5.495787675865802 2213 16 -5.67325395958208 2213 17 -6.138250117937623 2213 18 -7.097467116217114 2213 19 -4.412784264346758 2213 20 -4.542967535936278 2213 21 -6.475744004259621 2213 22 -10.59186078678267 2213 23 -8.56104164932291 2213 24 -9.002535441988574 2213 25 -6.205140475843596 2214 1 4.0620984917859815 2214 2 0.9775275505744325 2214 3 0.2403400419025452 2214 4 1.9334441600066512 2214 5 1.1974044184981771 2214 6 -0.703778036199914 2214 7 4.259779109779114 2214 8 1.3579600087412587 2214 9 4.393908868908859 2214 10 4.049635303932181 2214 11 3.1000777867965397 2214 12 2.8131284514096997 2214 13 -0.20188630813631647 2214 14 -1.0669986697330422 2214 15 2.281990101911976 2214 16 1.3045238181956975 2214 17 4.383972104284599 2214 18 5.246977328227327 2214 19 4.56499351343102 2214 20 6.068143575174833 2214 21 2.5798115512959363 2214 22 6.341472546550666 2214 23 3.7500694617882004 2214 24 2.0641312246780927 2214 25 3.694859524156403 2215 1 -2.4379015082140185 2215 2 -4.922472449425566 2215 3 -3.4596599580974576 2215 4 -5.666555839993347 2215 5 -6.002595581501826 2215 6 -6.303778036199915 2215 7 -6.140220890220885 2215 8 -2.342039991258744 2215 9 -2.5060911310911393 2215 10 -1.7503646960678196 2215 11 -2.3999222132034603 2215 12 -1.3868715485902996 2215 13 -4.801886308136318 2215 14 -4.866998669733039 2215 15 -6.418009898088023 2215 16 -8.395476181804302 2215 17 -5.016027895715403 2215 18 -9.153022671772671 2215 19 -7.035006486568978 2215 20 -5.831856424825165 2215 21 -6.620188448704063 2215 22 -7.5585274534493365 2215 23 -3.5499305382118003 2215 24 -9.335868775321906 2215 25 -6.905140475843595 2216 1 -5.837901508214017 2216 2 -6.704290631243751 2216 3 -5.2232963217338195 2216 4 -6.357464930902438 2216 5 -5.311686490592734 2216 6 -4.349232581654462 2216 7 -5.967493617493613 2216 8 -5.8784036276223794 2216 9 -6.124272949272957 2216 10 -6.404910150613272 2216 11 -6.318104031385278 2216 12 -6.414144275863027 2216 13 -5.429159035409043 2216 14 -6.512453215187588 2216 15 -4.899828079906207 2216 16 -5.804567090895212 2216 17 -6.734209713897219 2216 18 -5.834840853590855 2216 19 -5.7259155774780695 2216 20 -5.88640187937062 2216 21 -6.102006630522247 2216 22 -5.8948910898129725 2216 23 -5.1772032654845255 2216 24 -4.699505138958269 2216 25 -8.023322294025412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 813.0 3 0.0 4 12.0 5 131.0 6 120.0 7 98.0 8 73.0 9 46.0 10 47.0 11 45.0 12 32.0 13 30.0 14 35.0 15 38.0 16 26.0 17 38.0 18 40.0 19 42.0 20 47.0 21 68.0 22 77.0 23 84.0 24 104.0 25 127.0 26 117.0 27 118.0 28 131.0 29 172.0 30 230.0 31 236.0 32 296.0 33 325.0 34 388.0 35 432.0 36 458.0 37 561.0 38 835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.474864655839134 18.004640371229698 9.543696829079659 29.976798143851507 2 14.332096474953618 20.423623995052566 37.30674087816945 27.93753865182437 3 17.220590508579377 24.099551708146542 30.066470861029526 28.613386922244548 4 12.65451174289246 17.660692212608158 35.12051915945612 34.56427688504326 5 13.31891223733004 37.71631644004944 34.96600741656366 13.99876390605686 6 32.52675663099116 36.24941833410889 16.845044206607724 14.378780828292228 7 29.06167877569949 32.508888545370226 20.111300046375018 18.318132632555265 8 26.955177743431218 35.409582689335394 18.871715610510044 18.76352395672334 9 25.486862442040188 15.950540958268933 19.19629057187017 39.36630602782071 10 15.515376294235821 29.423582135682274 31.896152063050533 23.164889507031372 11 33.53941267387944 23.02936630602782 23.153013910355487 20.27820710973725 12 23.11495673671199 25.38627935723115 29.573547589616812 21.92521631644005 13 28.770086526576023 21.940667490729297 25.030902348578493 24.25834363411619 14 21.477132262051914 21.940667490729297 27.286773794808404 29.295426452410382 15 23.825710754017305 28.87824474660074 23.53213844252163 23.76390605686032 16 23.439431396786155 27.595797280593327 26.18974042027194 22.77503090234858 17 23.068603213844252 27.873918417799754 25.695302843016073 23.362175525339925 18 21.647095179233624 26.854140914709518 27.812113720642767 23.68665018541409 19 22.34239802224969 26.823238566131025 29.31087762669963 21.523485784919654 20 23.19221260815822 26.174289245982695 28.198393077873916 22.435105067985166 21 23.964771322620518 25.370828182941903 28.043881334981457 22.62051915945612 22 23.779357231149568 25.757107540173052 27.503090234857847 22.960444993819532 23 22.867737948084056 26.066131025957972 27.34857849196539 23.717552533992585 24 22.991347342398022 25.834363411619282 28.337453646477133 22.836835599505562 25 24.103831891223734 26.514215080346105 27.163164400494438 22.218788627935723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 2.0 2 1.5 3 1.0 4 1.0 5 1.0 6 5.5 7 10.0 8 10.0 9 10.0 10 13.0 11 16.0 12 16.0 13 16.0 14 17.5 15 19.0 16 19.0 17 19.0 18 44.0 19 69.0 20 69.0 21 69.0 22 94.5 23 120.0 24 120.0 25 120.0 26 183.0 27 246.0 28 246.0 29 246.0 30 309.0 31 372.0 32 372.0 33 372.0 34 451.0 35 530.0 36 530.0 37 530.0 38 605.5 39 681.0 40 681.0 41 681.0 42 700.5 43 720.0 44 720.0 45 720.0 46 789.0 47 858.0 48 858.0 49 858.0 50 857.0 51 856.0 52 856.0 53 856.0 54 794.0 55 732.0 56 732.0 57 732.0 58 653.5 59 575.0 60 575.0 61 575.0 62 456.0 63 337.0 64 337.0 65 337.0 66 257.0 67 177.0 68 177.0 69 177.0 70 140.5 71 104.0 72 104.0 73 104.0 74 68.0 75 32.0 76 32.0 77 32.0 78 22.0 79 12.0 80 12.0 81 12.0 82 7.5 83 3.0 84 3.0 85 3.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10815822002472188 2 0.061804697156983925 3 0.04635352286773795 4 0.0 5 0.0 6 0.3862793572311496 7 0.04635352286773795 8 0.030902348578491962 9 0.030902348578491962 10 0.015451174289245981 11 0.030902348578491962 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6472.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.09517923362175 #Duplication Level Percentage of deduplicated Percentage of total 1 98.63144493952896 95.76637824474659 2 1.161680458306811 2.2558714462299134 3 0.07956715467854869 0.23176761433868973 4 0.031826861871419476 0.12360939431396785 5 0.0 0.0 6 0.031826861871419476 0.1854140914709518 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.06365372374283895 1.4369592088998764 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTT 34 0.5253399258343634 No Hit GTATCAACGCAGAGTACTTTTTTTT 32 0.4944375772558714 No Hit TATCAACGCAGAGTACTTTTTTTTT 16 0.2472187886279357 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11 0.1699629171817058 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE