FastQCFastQC Report
Thu 26 May 2016
SRR1926822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616172
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15500.2515531377602358No Hit
GGTATCAACGCAGAGTACTTTTTTT9850.15985796173795627No Hit
TATCAACGCAGAGTACTTTTTTTTT7480.12139467551268153No Hit
GTACATGGGAAGCAGTGGTATCAAC6350.10305564030822563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTC508.462997E-515.2493443
GTCTAGG456.5494445E-414.8378551
GTGTTAA706.9479884E-613.6266021
CAATACT801.9455547E-613.091024
CTTACTC603.975545E-412.7077863
AATACTG604.019555E-412.6912195
GTATAGG802.7434062E-511.9232761
TATACTG752.0330216E-411.4220975
GGACTTC600.005784805411.1048166
TAAGGTG600.005784805411.1048165
TAGGACC1408.023562E-910.8808484
GGCGAGG1752.419256E-1010.29652519
TGTAGGA3350.010.2420962
GTAAGAC750.002573522310.1662293
TTATACA1605.9135346E-910.11578754
ATGTCCA856.685998E-410.0415039
GTCCTAA1909.094947E-1110.0406541
GTCCAGG951.5743318E-410.0406541
TAAGACT951.6044406E-410.0218334
GTATCAA9800.09.6359551
Produced by FastQC (version 0.11.5)