##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926822_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	616172
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.426809397376058	31.0	28.0	33.0	20.0	34.0
2	28.510766474296137	31.0	30.0	33.0	20.0	34.0
3	28.289076751296715	31.0	28.0	33.0	16.0	34.0
4	31.813350817628844	35.0	32.0	37.0	19.0	37.0
5	32.042467038424334	35.0	33.0	37.0	19.0	37.0
6	31.2450322312601	35.0	32.0	37.0	17.0	37.0
7	31.259589854780806	35.0	32.0	37.0	17.0	37.0
8	31.067555812338114	35.0	32.0	37.0	11.0	37.0
9	33.19670157034075	37.0	33.0	39.0	17.0	39.0
10	33.395764494329505	38.0	34.0	39.0	17.0	39.0
11	33.53509896587317	38.0	34.0	39.0	19.0	39.0
12	33.40329161338068	38.0	34.0	39.0	17.0	39.0
13	33.506902293515445	38.0	34.0	39.0	18.0	39.0
14	34.5567877151185	39.0	34.0	41.0	18.0	41.0
15	34.10047194614491	39.0	33.0	41.0	16.0	41.0
16	34.19434346253968	39.0	34.0	41.0	17.0	41.0
17	34.43913550112631	39.0	34.0	41.0	17.0	41.0
18	34.503189044617415	39.0	34.0	41.0	17.0	41.0
19	33.89198632849269	39.0	34.0	41.0	10.0	41.0
20	34.155161221217455	39.0	34.0	41.0	11.0	41.0
21	34.14506014554377	39.0	34.0	41.0	10.0	41.0
22	34.16169348818187	39.0	34.0	41.0	10.0	41.0
23	34.17076887622287	39.0	34.0	41.0	2.0	41.0
24	32.891246275390635	39.0	32.0	41.0	2.0	41.0
25	33.46921476470855	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.3496581386441733
1101	2	-0.5279859754471836
1101	3	-1.4066266506667908
1101	4	-0.8221454573767453
1101	5	-0.5043643450725597
1101	6	-2.689424552871138
1101	7	-1.0280603972642552
1101	8	-0.35257247885996534
1101	9	-0.45064658842346716
1101	10	-9.007266202954003
1101	11	-3.829375811273593
1101	12	-1.8595175143095695
1101	13	-1.2018893553646066
1101	14	-0.852350386146675
1101	15	-1.9907450078699398
1101	16	-0.6425727077001824
1101	17	-0.4426587136936604
1101	18	-0.3822359201886485
1101	19	-1.1073308385870675
1101	20	-0.5267459370076963
1101	21	-0.549988807668214
1101	22	-0.39673122166365005
1101	23	-0.5048468500470378
1101	24	-0.3988831453614594
1101	25	-0.20014493431183666
1102	1	0.09629682382454874
1102	2	0.010848469923956827
1102	3	0.008594366847805901
1102	4	0.004699830363037449
1102	5	0.37113607194162057
1102	6	-0.6179170466159256
1102	7	0.21538205477911276
1102	8	0.8113557947013383
1102	9	0.6422850212512614
1102	10	0.6261470580968691
1102	11	0.45266130298828955
1102	12	0.543766472345979
1102	13	0.2542094769956975
1102	14	-0.11853053627177701
1102	15	0.6840881864586663
1102	16	0.4719602364199176
1102	17	0.18171576503861786
1102	18	0.019484263297592008
1102	19	0.6650106960251065
1102	20	0.4670614024418427
1102	21	0.43197532911243997
1102	22	0.1968259092788074
1102	23	-0.1311291686458702
1102	24	-3.4184202596233426
1102	25	-1.4018129910257642
1103	1	0.18370271162927665
1103	2	-0.005832688892649429
1103	3	0.265468592342188
1103	4	0.05640035832854906
1103	5	-0.32111184275081683
1103	6	0.3167680485115909
1103	7	0.2372231132093816
1103	8	0.4286497892238259
1103	9	0.3861813963046288
1103	10	0.4213183084483276
1103	11	0.05173423928878407
1103	12	0.2612736837092129
1103	13	0.2097567595874139
1103	14	0.22172865626381366
1103	15	0.7899793755786249
1103	16	0.8573034660692969
1103	17	0.4006366118661404
1103	18	0.07955465869897438
1103	19	-1.3738577707922062
1103	20	-0.16999224801791257
1103	21	0.154143892775501
1103	22	0.25840756690306677
1103	23	0.2604934141155866
1103	24	1.7322230356312147
1103	25	0.8608446436470985
1104	1	-0.21484660871069394
1104	2	-0.09187178475982449
1104	3	-1.5412718834827537
1104	4	-0.770066743407785
1104	5	-0.25624904573775
1104	6	-1.6561374131815576
1104	7	-0.38202381189840295
1104	8	0.07830335262562471
1104	9	-0.07658893875606765
1104	10	-0.0019169790072197657
1104	11	-0.07363856071926733
1104	12	0.04976740808510982
1104	13	-0.268934809910057
1104	14	-0.28937921098037833
1104	15	0.3307627526622099
1104	16	-0.16518911568244476
1104	17	-0.2034680263322386
1104	18	-0.46602195123077905
1104	19	-1.2490436988974878
1104	20	-0.8254155600675688
1104	21	-0.3853546613267511
1104	22	-0.008815478869856008
1104	23	0.03819638720352003
1104	24	1.1189660785853306
1104	25	0.4485246887480301
1105	1	0.38198768032559016
1105	2	0.19436825482958042
1105	3	0.6161080090396993
1105	4	0.2688091865708593
1105	5	0.23794827188794443
1105	6	-0.24132670001976209
1105	7	0.20025512524140865
1105	8	0.41237138009798
1105	9	-0.37095585662006414
1105	10	0.062145547267604684
1105	11	-0.013458102222081436
1105	12	-0.04485559172510989
1105	13	0.070472261891382
1105	14	-0.42403363247780845
1105	15	0.0051182837679988324
1105	16	-0.5636896189579375
1105	17	-0.22428729637857714
1105	18	0.01322724340831627
1105	19	-0.9825635284324363
1105	20	-0.2570817983294731
1105	21	-0.16357592477645255
1105	22	-0.17178785517098305
1105	23	-0.2964121509433255
1105	24	-4.494554375539728
1105	25	-2.229973556393979
1106	1	-0.014666528733997097
1106	2	-0.8659893808365844
1106	3	-0.22991408527344248
1106	4	-0.518178672261751
1106	5	-0.15988699831508413
1106	6	-0.05893274554708228
1106	7	-2.726968451750487
1106	8	-0.1967314957388524
1106	9	-0.5254960603413465
1106	10	-0.2234430364353983
1106	11	-0.7440597023011293
1106	12	-0.14279779266313852
1106	13	-0.2680464474581754
1106	14	-0.483393718492934
1106	15	0.18031411758658322
1106	16	0.19784778386037516
1106	17	-0.21066039171162032
1106	18	-0.3167228908726898
1106	19	0.20513461398424937
1106	20	0.1337318045775362
1106	21	-0.1580482292455514
1106	22	-0.5137038798415148
1106	23	-0.41814144090677274
1106	24	-3.8606786245836453
1106	25	-1.9699554169887676
1107	1	0.17065053818624065
1107	2	-0.0016237507153959996
1107	3	0.3935474505942693
1107	4	0.4386229408916513
1107	5	0.1998893722246322
1107	6	0.3149115096171009
1107	7	-2.002728663780349
1107	8	-1.6827108187809117
1107	9	-0.3439083990765823
1107	10	0.0487732286289031
1107	11	-0.4016490090902707
1107	12	-0.1671097409235358
1107	13	-0.16485048843443195
1107	14	-0.2584862792390865
1107	15	-1.6839503532491946
1107	16	-0.7920362875985418
1107	17	-0.5191456325718846
1107	18	-0.7227897504163963
1107	19	0.3101851599071921
1107	20	0.19871990150161878
1107	21	0.02383953789926352
1107	22	-0.20082495401825184
1107	23	-0.14069524078673368
1107	24	-3.806345972389501
1107	25	-1.8648070837565918
1108	1	0.6948559679388993
1108	2	0.3145558218359916
1108	3	0.8472134747250593
1108	4	1.0461016690600395
1108	5	0.9912469401938964
1108	6	-2.2870342707394755
1108	7	-0.6560253920395951
1108	8	0.7704943967931186
1108	9	0.6485174659129953
1108	10	0.9518638910898858
1108	11	0.9332809285383163
1108	12	1.0058038023048539
1108	13	0.9275124210199337
1108	14	0.8992692585764175
1108	15	-0.16420373305280123
1108	16	0.9301545650270882
1108	17	0.6008365841119812
1108	18	0.8042713943359061
1108	19	1.19738441742129
1108	20	-0.3834857489199237
1108	21	0.49833930100472656
1108	22	0.809799603832694
1108	23	0.8330711227847303
1108	24	0.06908968640448165
1108	25	0.5891445118741601
1109	1	0.43200725478592616
1109	2	0.25061410094700776
1109	3	-0.718238568161059
1109	4	0.26600389327260743
1109	5	0.39487171298702606
1109	6	0.8723497022854723
1109	7	0.7823005653025881
1109	8	1.2235734340506532
1109	9	0.6277911503083828
1109	10	0.751108510567768
1109	11	0.4905535240969172
1109	12	0.3358892847736996
1109	13	0.4184219509761178
1109	14	0.30204228001069566
1109	15	-0.9876892401082884
1109	16	0.10968092102403659
1109	17	0.1244726843200894
1109	18	0.31870945796261196
1109	19	-1.2263816407261103
1109	20	-0.10489719751190307
1109	21	0.23629843450138566
1109	22	0.27423898460067875
1109	23	0.14963175957863228
1109	24	-0.538245253841211
1109	25	-0.11328473568693198
1110	1	0.44886516546288746
1110	2	0.48601379381908316
1110	3	0.92451086663824
1110	4	0.6721496510681071
1110	5	0.8456587283007693
1110	6	0.8555973668335248
1110	7	1.0146311117343636
1110	8	1.3468852968668479
1110	9	0.9846556727671185
1110	10	1.1462467181350604
1110	11	0.7707107138763831
1110	12	0.9362081432815685
1110	13	0.8827033995961742
1110	14	0.8935194800277628
1110	15	1.1492642214793918
1110	16	0.9990978045287022
1110	17	0.41727437352368923
1110	18	0.8339442828570327
1110	19	-1.6698423752737312
1110	20	0.3164012711141311
1110	21	0.37670708527133456
1110	22	0.5379019117004518
1110	23	0.40155024270792694
1110	24	-0.08113972127285507
1110	25	-0.05106786924492468
1111	1	0.24357184025684475
1111	2	-0.226379471456724
1111	3	0.5750745694047623
1111	4	0.501642497856718
1111	5	0.420404024801762
1111	6	0.7057123611734468
1111	7	0.47177792032128707
1111	8	0.757928851293233
1111	9	0.30943964219757447
1111	10	0.09032002766704039
1111	11	-0.01541722949210822
1111	12	0.08628676970107563
1111	13	-0.07445723904499602
1111	14	-0.0397735583327119
1111	15	-1.149354309768846
1111	16	-0.48173425251258806
1111	17	-0.3349965726056823
1111	18	-0.062394850562007775
1111	19	0.4719474386352047
1111	20	-0.2630728794900392
1111	21	-0.16568920212743876
1111	22	0.045930002077291476
1111	23	-0.22392881500437767
1111	24	0.43058662805382397
1111	25	0.27738465012996016
1112	1	0.3321600431740066
1112	2	0.4042109942497838
1112	3	-1.1866771557172946
1112	4	0.07267777494648797
1112	5	0.3249285842203662
1112	6	1.378378477431891
1112	7	1.2644901077862478
1112	8	1.3557608544733704
1112	9	0.14632310854622688
1112	10	0.5514459182581177
1112	11	0.3417100473122687
1112	12	0.7080329907409393
1112	13	0.33914599817074986
1112	14	0.59007385627757
1112	15	1.1334974163724851
1112	16	0.8453060801786023
1112	17	-0.019678915713861045
1112	18	0.4421327666800394
1112	19	1.2804216866654556
1112	20	0.8136581033963424
1112	21	1.0818293741499687
1112	22	0.8896324146999888
1112	23	0.7397238570236695
1112	24	2.1248542283759164
1112	25	1.187228803061906
1113	1	-0.643062773225278
1113	2	-0.02368561894094512
1113	3	-5.201501873483188
1113	4	-1.3124975072876168
1113	5	-0.21969411334350397
1113	6	0.6476298143302905
1113	7	0.562202526086903
1113	8	1.0718927617817435
1113	9	0.34578834918794144
1113	10	0.3413746170103451
1113	11	0.14132383222016642
1113	12	-0.10717794211705467
1113	13	-0.5544071806765487
1113	14	-0.8553806891769753
1113	15	-0.1764847583155742
1113	16	0.11500304987557541
1113	17	-0.3063396406098775
1113	18	-0.2055202339141431
1113	19	0.6363634573131094
1113	20	0.03403837671778831
1113	21	-0.009079716759117673
1113	22	0.2383846428639842
1113	23	-0.0420349070880377
1113	24	1.5174270150663531
1113	25	0.7542222671141872
1114	1	0.47002862310226234
1114	2	0.3822259853542853
1114	3	0.5437386481345037
1114	4	0.48663155723521
1114	5	0.6282894304108595
1114	6	1.545853030469491
1114	7	1.5192436100854714
1114	8	1.5760455104654376
1114	9	0.5118435681956583
1114	10	0.629586321326336
1114	11	0.5055758467892275
1114	12	-0.06999852752157665
1114	13	0.20713483747816497
1114	14	0.44689714207101616
1114	15	-1.9126979346351831
1114	16	0.11503513200582915
1114	17	0.45624976473840206
1114	18	0.6025210576746787
1114	19	1.1835005989840184
1114	20	0.9994330812791148
1114	21	0.8871666740012287
1114	22	0.8325145566196639
1114	23	0.45578728242299604
1114	24	1.4559991715516603
1114	25	1.0340794072753567
1115	1	-3.6831621932749883
1115	2	-0.9344146993054849
1115	3	0.09519793320004766
1115	4	1.018032732977499
1115	5	0.9363953647012337
1115	6	1.8507643075642015
1115	7	1.5543179638112896
1115	8	1.6629311489676049
1115	9	1.162883074401968
1115	10	-0.00459334764030217
1115	11	0.5121468092983221
1115	12	0.37442934868658995
1115	13	0.773830875109148
1115	14	0.7756052263159816
1115	15	1.2405823502179842
1115	16	1.151153284617358
1115	17	0.9343259213895649
1115	18	0.6900058638489099
1115	19	-1.8264782991077695
1115	20	-0.060548753909110076
1115	21	0.2594648841798488
1115	22	0.7298372802569659
1115	23	0.6838695391975236
1115	24	-0.8586569396422945
1115	25	-0.0551812125875486
1116	1	0.3556166239067089
1116	2	-0.7879927597077021
1116	3	-0.1853071119965044
1116	4	-0.33727435832653896
1116	5	0.44855288694915174
1116	6	1.0218542802360524
1116	7	0.8051824792622035
1116	8	-1.9782169836089452
1116	9	-0.4010875653569812
1116	10	-0.38979673212632093
1116	11	0.19816828641975803
1116	12	-0.006837731405902048
1116	13	-0.1564008886074788
1116	14	-0.03834088818194914
1116	15	-0.647376622523943
1116	16	-0.2880442138060175
1116	17	-0.17298435819840563
1116	18	0.058808855930756465
1116	19	0.8231135304767037
1116	20	0.2284779435893185
1116	21	0.1295396862259537
1116	22	0.3327803115792278
1116	23	0.4977990115540507
1116	24	1.3827145479899698
1116	25	0.7173075758645595
1201	1	0.5709421636663414
1201	2	0.3227886952502885
1201	3	0.6700900411924202
1201	4	-0.012117925526172968
1201	5	0.4102113405245049
1201	6	0.8565185479710173
1201	7	0.6783987908160718
1201	8	0.8555521162598794
1201	9	0.3019338477674154
1201	10	0.6635743282487567
1201	11	0.6471060500954451
1201	12	0.5266490803784052
1201	13	0.5272215278139782
1201	14	0.6583168563495505
1201	15	1.124348924326135
1201	16	0.7875287822587893
1201	17	0.4102618218278238
1201	18	0.23436146698474403
1201	19	-0.558888277585126
1201	20	0.2815676021847011
1201	21	0.6697801407230628
1201	22	0.6439619331625224
1201	23	0.47251495085989603
1201	24	-0.732817500792251
1201	25	0.08868037339708223
1202	1	0.014744928168532567
1202	2	-0.2314224486125731
1202	3	-0.2208380416854112
1202	4	-0.38834206197696375
1202	5	0.0916926100205373
1202	6	0.559028349648031
1202	7	0.4011701613337735
1202	8	0.8150890764521925
1202	9	0.5225188004045691
1202	10	0.6130377400909026
1202	11	0.4285787341809524
1202	12	0.4924676992720336
1202	13	0.32580779688073847
1202	14	0.5462257365258338
1202	15	0.9937018705528473
1202	16	0.7194207205583041
1202	17	0.6511255141267114
1202	18	0.4383966099318286
1202	19	-0.04165449685650913
1202	20	0.5696396051609725
1202	21	0.4897154639637691
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2211	4	0.351465653734369
2211	5	0.29159525088703475
2211	6	-2.2337427346893186
2211	7	-0.07288362958748706
2211	8	0.31434671305922635
2211	9	0.3473332095563251
2211	10	0.46457723138942697
2211	11	0.3346393402415586
2211	12	0.391871374579317
2211	13	0.04722680625155817
2211	14	0.2355284017321182
2211	15	0.6345075173825876
2211	16	-0.22439088951836794
2211	17	0.07022007418512999
2211	18	0.12193074647801438
2211	19	0.38749239373616007
2211	20	-0.10048331074507644
2211	21	-0.35554436322376404
2211	22	-3.2063271812596064
2211	23	-1.736033718733907
2211	24	0.4046522081738999
2211	25	0.04480456063765814
2212	1	-0.5105418764203407
2212	2	-0.11253414639973869
2212	3	-3.054661782865267
2212	4	-1.7475846098577286
2212	5	-0.527037289657855
2212	6	0.29016164778080267
2212	7	0.5731665132754031
2212	8	0.27476363124506875
2212	9	0.23504700085577923
2212	10	0.6493935217834093
2212	11	0.49232375410127105
2212	12	0.3921892948175625
2212	13	0.0539554472431476
2212	14	-0.09369410219158425
2212	15	-0.7442763370984764
2212	16	0.052121243766677594
2212	17	0.2166585626554891
2212	18	0.3178138806081634
2212	19	1.1173024464545804
2212	20	-0.3363076899957491
2212	21	-0.2877649757232632
2212	22	0.3273542546130628
2212	23	0.28084221188704817
2212	24	1.5369372095824048
2212	25	0.9260955582633201
2213	1	-0.6793656180626364
2213	2	-0.48974591589209737
2213	3	0.03490864448122366
2213	4	-7.729832880743359
2213	5	-2.1610292601189762
2213	6	-3.831045239506274
2213	7	-1.455869558238156
2213	8	-3.0061589213192406
2213	9	-0.840066802121143
2213	10	-0.2981074185784536
2213	11	-0.24772184385373208
2213	12	-0.07638342777952545
2213	13	-0.08983163947740991
2213	14	-0.07811672000548953
2213	15	-3.0242009546206816
2213	16	-1.1350932892381067
2213	17	-0.6131570496544256
2213	18	-0.4223852458148514
2213	19	0.29535132991880175
2213	20	-1.1997709851772527
2213	21	-0.24183501541037344
2213	22	-0.34662612444872565
2213	23	-0.19768491275504374
2213	24	-0.7344147621069439
2213	25	-0.32863503765742763
2214	1	0.33879116040115065
2214	2	0.11252210918341632
2214	3	-0.09488028445022323
2214	4	0.1852328431306951
2214	5	0.3460097841095191
2214	6	0.23361771942610332
2214	7	0.7302317245212429
2214	8	-2.461884855225321
2214	9	-1.1039621470370449
2214	10	-0.5179729630812915
2214	11	0.020820929072151273
2214	12	-1.1245912650621293
2214	13	-0.3098740031671312
2214	14	-0.40801392790292823
2214	15	-1.6323550534534945
2214	16	-0.4796674010862674
2214	17	-0.3253993174607501
2214	18	-0.5305448132823116
2214	19	0.5280426455446943
2214	20	-1.3819020391836716
2214	21	-0.779562214393934
2214	22	-0.17972339505317336
2214	23	-0.6166114926891666
2214	24	-2.2273556662180845
2214	25	-1.2289700826734062
2215	1	0.4140938223829913
2215	2	-0.17760718756839822
2215	3	0.054423221350383244
2215	4	0.734393020040482
2215	5	0.5777841150050591
2215	6	1.19132538520169
2215	7	0.6213172346406246
2215	8	-0.5289968038537722
2215	9	-0.0353464480552077
2215	10	-0.2853470798427864
2215	11	-0.212656506509326
2215	12	-0.4451968554044399
2215	13	-0.15564936692434372
2215	14	-0.06373672914064343
2215	15	0.1271643307478456
2215	16	0.2478822506021885
2215	17	-0.21518988370439018
2215	18	-0.890784966510175
2215	19	0.15782790140136882
2215	20	-3.449270914301067
2215	21	-2.2417772644430443
2215	22	-1.1077418731375133
2215	23	-0.9149151763619585
2215	24	-0.9506501340494253
2215	25	-0.6545211212493314
2216	1	-0.6567865142828069
2216	2	-1.2486746868250123
2216	3	-0.5092724214882978
2216	4	-0.48965113412640093
2216	5	-0.7288473553718049
2216	6	-1.5762940281772764
2216	7	-10.430147616467888
2216	8	-10.676485786783086
2216	9	-5.714558679900236
2216	10	-3.4903241463487014
2216	11	-2.5616085985576724
2216	12	-2.190018892948771
2216	13	-1.91900374187016
2216	14	-1.9901413195665114
2216	15	-3.344730897092223
2216	16	-2.1794067033209785
2216	17	-2.16049384466438
2216	18	-2.026448676656088
2216	19	-1.3903502611805365
2216	20	-2.3409297015036863
2216	21	-4.746100234154305
2216	22	-3.3764611705729024
2216	23	-3.167817209304193
2216	24	-3.4260058442504366
2216	25	-2.9339824170788447
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34407.0
3	5.0
4	256.0
5	1621.0
6	2371.0
7	1836.0
8	1553.0
9	1349.0
10	1228.0
11	1225.0
12	1185.0
13	1034.0
14	1081.0
15	1197.0
16	1240.0
17	1287.0
18	1498.0
19	1779.0
20	2328.0
21	3125.0
22	3993.0
23	4583.0
24	5480.0
25	6142.0
26	7095.0
27	7813.0
28	8661.0
29	10165.0
30	12055.0
31	14888.0
32	19385.0
33	25533.0
34	34542.0
35	48332.0
36	70317.0
37	113593.0
38	161990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.529964888771076	20.08170394354882	13.94106062256974	24.447270545110367
2	13.74961815192029	21.46051203390161	42.07364630793529	22.716223506242812
3	18.054986838256802	26.03344057716681	30.815703097071918	25.09586948750447
4	12.063933896430596	18.164027926419504	39.71638346696202	30.05565471018788
5	12.418490567875422	38.32327164291524	36.58067019136962	12.677567597839726
6	28.905936760907924	38.37524328658876	20.2180691932219	12.500750759281415
7	25.9493907909541	32.63904832152039	23.437818017217097	17.973742870308406
8	23.791520740628577	36.71621232177485	22.54172629892491	16.95054063867166
9	25.431982534026993	16.988580565046952	21.42893085844608	36.150506042479975
10	14.304152163748766	30.08045872512858	34.96366694373734	20.65172216738532
11	32.31945150069552	24.276677904366473	25.22233277767047	18.181537817267536
12	22.40005194214943	26.35999155940072	31.829824532926455	19.410131965523398
13	27.226452365841503	23.57709457035682	26.52768061342583	22.668772450375844
14	20.32671558959925	23.583645391521433	28.54930681690909	27.54033220197023
15	22.51080213348223	31.329108787466602	24.17811265383504	21.981976425216125
16	21.00334545879534	29.788056014115533	28.224407077893517	20.984191449195613
17	20.592665697070434	29.868812331186373	28.28845704342406	21.250064928319134
18	20.5215189462493	29.20717108840501	29.870553576211073	20.400756389134617
19	22.575811931542404	28.238838629874145	28.486391106941046	20.6989583316424
20	22.233728795126552	28.569179301803523	28.964424670453013	20.23266723261691
21	22.21571154489175	27.898072009894786	28.312951847319024	21.573264597894436
22	21.71477545534672	28.867383361279337	28.51563704714894	20.902204136225
23	21.35676801386862	28.551926981967867	28.53764282131651	21.553662182846995
24	22.03481718946557	28.117355841415414	28.892261494136267	20.955565474982755
25	21.751628852190795	28.172731374923305	28.755774431159693	21.31986534172621
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	380.0
1	380.0
2	491.0
3	602.0
4	602.0
5	602.0
6	850.5
7	1099.0
8	1099.0
9	1099.0
10	1563.5
11	2028.0
12	2028.0
13	2028.0
14	3319.5
15	4611.0
16	4611.0
17	4611.0
18	7575.5
19	10540.0
20	10540.0
21	10540.0
22	15678.5
23	20817.0
24	20817.0
25	20817.0
26	28408.5
27	36000.0
28	36000.0
29	36000.0
30	44890.5
31	53781.0
32	53781.0
33	53781.0
34	59867.5
35	65954.0
36	65954.0
37	65954.0
38	69907.5
39	73861.0
40	73861.0
41	73861.0
42	75852.5
43	77844.0
44	77844.0
45	77844.0
46	78308.5
47	78773.0
48	78773.0
49	78773.0
50	73687.5
51	68602.0
52	68602.0
53	68602.0
54	59438.5
55	50275.0
56	50275.0
57	50275.0
58	41857.0
59	33439.0
60	33439.0
61	33439.0
62	26726.0
63	20013.0
64	20013.0
65	20013.0
66	15156.5
67	10300.0
68	10300.0
69	10300.0
70	7390.5
71	4481.0
72	4481.0
73	4481.0
74	3154.5
75	1828.0
76	1828.0
77	1828.0
78	1236.5
79	645.0
80	645.0
81	645.0
82	436.5
83	228.0
84	228.0
85	228.0
86	139.0
87	50.0
88	50.0
89	50.0
90	31.5
91	13.0
92	13.0
93	13.0
94	10.5
95	8.0
96	8.0
97	8.0
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2059489882695092
2	0.12074550612491317
3	0.12204384490044988
4	0.066702154593198
5	0.022558636224950177
6	0.0209357127555293
7	0.3270190790883065
8	0.3231240627616964
9	0.018988204592224247
10	0.033107638776185874
11	0.01476860357172997
12	0.016553819388092937
13	0.01476860357172997
14	0.016391527041150846
15	0.014606311224787883
16	0.0183390352044559
17	0.017527573469745462
18	0.014119434183961622
19	0.02288322091883435
20	0.01606694234726667
21	0.014606311224787883
22	0.016716111735035025
23	0.0170406964289192
24	0.0157423576533825
25	0.015255480612556235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	616172.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.04183748712276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.25162859232316	81.16793101342523
2	5.317235585967619	9.256439115090789
3	0.7542930904619926	1.9696516979295704
4	0.2326683605252875	0.8100752650094948
5	0.11422021642386786	0.49709687578551476
6	0.07780086881402143	0.4063158347800207
7	0.046875950905182144	0.28561182305202465
8	0.03268589388938228	0.22760322112327608
9	0.025945999065007892	0.20325486906517107
>10	0.1242451926392425	1.9857405576351406
>50	0.010640191031146154	0.6403359127007258
>100	0.010640052435087208	1.723738621702835
>500	9.33337932521364E-4	0.5743629019066584
>1k	1.8666758650427278E-4	0.2518422907935843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1550	0.2515531377602358	No Hit
GGTATCAACGCAGAGTACTTTTTTT	985	0.15985796173795627	No Hit
TATCAACGCAGAGTACTTTTTTTTT	748	0.12139467551268153	No Hit
GTACATGGGAAGCAGTGGTATCAAC	635	0.10305564030822563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	1.622923469420876E-4	0.0	0.0	0.0	0.0
13	1.622923469420876E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATTC	50	8.462997E-5	15.249344	3
GTCTAGG	45	6.5494445E-4	14.837855	1
GTGTTAA	70	6.9479884E-6	13.626602	1
CAATACT	80	1.9455547E-6	13.09102	4
CTTACTC	60	3.975545E-4	12.707786	3
AATACTG	60	4.019555E-4	12.691219	5
GTATAGG	80	2.7434062E-5	11.923276	1
TATACTG	75	2.0330216E-4	11.422097	5
GGACTTC	60	0.0057848054	11.104816	6
TAAGGTG	60	0.0057848054	11.104816	5
TAGGACC	140	8.023562E-9	10.880848	4
GGCGAGG	175	2.419256E-10	10.296525	19
TGTAGGA	335	0.0	10.242096	2
GTAAGAC	75	0.0025735223	10.166229	3
TTATACA	160	5.9135346E-9	10.1157875	4
ATGTCCA	85	6.685998E-4	10.041503	9
GTCCTAA	190	9.094947E-11	10.040654	1
GTCCAGG	95	1.5743318E-4	10.040654	1
TAAGACT	95	1.6044406E-4	10.021833	4
GTATCAA	980	0.0	9.635955	1
>>END_MODULE