FastQCFastQC Report
Thu 26 May 2016
SRR1926822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616172
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26050.4227715637841382No Hit
GGTATCAACGCAGAGTACTTTTTTT16700.2710282193932863No Hit
TATCAACGCAGAGTACTTTTTTTTT13200.21422589796355565No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8370.13583869439052731No Hit
GTACATGGGAAGCAGTGGTATCAAC7560.12269301428821822No Hit
CCCATGTACTCTGCGTTGATACCAC6680.10841128775731451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGT250.00606729718.9786558
AATACCT508.543816E-515.2323715
GGCGAGG1900.012.98539619
TAGGACC1900.012.0255574
TCTAGAC752.0263536E-411.426143
GAGGTGT752.0294222E-411.4242796
CTGGACT752.0294222E-411.4242794
TGGCGAG4100.011.34090318
ATGGCGA4700.010.90263317
TATGGCG4800.010.67549416
GGACGTG3700.010.5494486
AGGACGT3900.010.4966675
CTGTAGG4000.010.4824971
TGTAGGA4000.010.4807892
TCAGGAC1002.3321929E-510.4739613
GTACTGT1002.3363904E-510.4722566
GACGTGA2650.010.3862337
TTAGGAC3700.010.293823
CTCCTAC750.002576434510.1648461
CTAGACA856.453381E-410.0802474
Produced by FastQC (version 0.11.5)