##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926822_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616172 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.495760923897873 31.0 30.0 33.0 25.0 34.0 2 29.773577832163745 31.0 30.0 33.0 26.0 34.0 3 29.78059859909246 31.0 30.0 34.0 25.0 34.0 4 33.394862148880506 35.0 35.0 37.0 28.0 37.0 5 33.32293904948618 35.0 35.0 37.0 28.0 37.0 6 33.28967885590387 37.0 35.0 37.0 28.0 37.0 7 33.266579786163604 37.0 35.0 37.0 27.0 37.0 8 33.30939088436346 37.0 35.0 37.0 28.0 37.0 9 34.771463162883094 39.0 35.0 39.0 27.0 39.0 10 34.6470222600183 39.0 35.0 39.0 27.0 39.0 11 34.69756171977954 39.0 35.0 39.0 27.0 39.0 12 34.596081938160125 39.0 35.0 39.0 27.0 39.0 13 34.6030994592419 39.0 35.0 39.0 27.0 39.0 14 35.635648812344606 39.0 36.0 41.0 27.0 41.0 15 35.60708373635933 39.0 36.0 41.0 27.0 41.0 16 35.57809832319547 39.0 36.0 41.0 27.0 41.0 17 35.56125400050635 39.0 36.0 41.0 27.0 41.0 18 35.53734346903138 40.0 36.0 41.0 27.0 41.0 19 35.61795083191057 40.0 36.0 41.0 27.0 41.0 20 35.57482488655765 40.0 36.0 41.0 27.0 41.0 21 35.522651142862706 40.0 36.0 41.0 27.0 41.0 22 35.43053725258532 40.0 36.0 41.0 26.0 41.0 23 35.35192770849698 40.0 36.0 41.0 26.0 41.0 24 35.26958706335244 40.0 36.0 41.0 25.0 41.0 25 35.1609810247788 39.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1685525940651118 1101 2 -0.003981802370947918 1101 3 0.253608702550153 1101 4 0.2014637852295209 1101 5 0.08986757985840654 1101 6 0.4220428796766953 1101 7 0.4329543311005324 1101 8 0.3385784863633887 1101 9 0.3870118455546532 1101 10 0.2609075473375313 1101 11 0.4367539907831741 1101 12 0.3638143910150262 1101 13 0.6575072258574082 1101 14 0.2576775270488696 1101 15 0.2309206884104782 1101 16 0.46973124722474324 1101 17 0.3978415140131091 1101 18 0.1980561110518977 1101 19 0.5244648695281455 1101 20 0.5028638471789577 1101 21 0.45242344583994765 1101 22 0.30565767478187666 1101 23 0.26527009064676577 1101 24 0.6078094780173089 1101 25 0.6514727926429202 1102 1 -0.925089291312819 1102 2 -0.9981331785177368 1102 3 -0.8642707803522676 1102 4 -0.9630378828271944 1102 5 -1.0682746219764567 1102 6 -0.9720042429254718 1102 7 -0.8166495054299148 1102 8 -0.9999014969560491 1102 9 -1.2436178458548497 1102 10 -1.1048764393180193 1102 11 -1.3421867932034814 1102 12 -1.2216735155737979 1102 13 -0.9228409809816256 1102 14 -1.1895806047276096 1102 15 -1.1285559587955305 1102 16 -1.2236799287552458 1102 17 -1.103701438447274 1102 18 -0.9592207863626143 1102 19 -1.0235960145419085 1102 20 -1.2408705147893428 1102 21 -1.0067508660866622 1102 22 -1.1235750191297171 1102 23 -1.4551948801622387 1102 24 -1.336279346002705 1102 25 -1.3161981831702576 1103 1 -0.4730787007620485 1103 2 -0.6200572847773032 1103 3 -0.29466159979222795 1103 4 -0.30088504358071333 1103 5 -0.6563157345570332 1103 6 -0.6831487930093019 1103 7 -0.41797436562839607 1103 8 -0.47936212864022565 1103 9 -0.35661626299967253 1103 10 -0.5005902341091755 1103 11 -0.43168579871223045 1103 12 -0.6202171846737556 1103 13 -0.3370921446925834 1103 14 -0.423223566749499 1103 15 -0.3171122803233999 1103 16 -0.6109080056903338 1103 17 -0.4227678142295872 1103 18 -0.47473823421690753 1103 19 -0.6209793570738498 1103 20 -0.3337513112154227 1103 21 -0.5009817754994401 1103 22 -0.43862568090897014 1103 23 -0.2268914509898039 1103 24 -0.5838412804183548 1103 25 -0.45837268254023655 1104 1 -0.6234583590318543 1104 2 -0.5979673899540963 1104 3 -0.19492233957845073 1104 4 -0.22151293317402576 1104 5 -0.18011579042984494 1104 6 -0.2850801801902634 1104 7 -0.5079547598085625 1104 8 -0.3535778329270798 1104 9 -0.25932961785997577 1104 10 -0.3159771533276583 1104 11 -0.10326818216582723 1104 12 -0.17110800366346268 1104 13 -0.11003712004059452 1104 14 -0.4144122733945892 1104 15 -0.23135758209950552 1104 16 -0.31513571508124727 1104 17 -0.24190349707335912 1104 18 -0.030436128415956887 1104 19 -0.14959278013925825 1104 20 -0.199214866789994 1104 21 -0.28582489118888077 1104 22 -0.26163722543984846 1104 23 -0.32350485392085915 1104 24 -0.10790559958801538 1104 25 -0.19001833817748093 1105 1 -0.030267111941604696 1105 2 -0.2637732433243585 1105 3 -0.034716176795853926 1105 4 -0.23784356235247373 1105 5 0.01764385288115733 1105 6 -0.2539876039242088 1105 7 -0.42916031481693295 1105 8 -0.6040480772138821 1105 9 -0.4893301223366038 1105 10 -0.2838600090497465 1105 11 -0.2870862534796004 1105 12 -0.23647616350088185 1105 13 -0.31909944212249997 1105 14 -0.2944092945802055 1105 15 -0.374857160501179 1105 16 -0.33401395123876654 1105 17 -0.5340579245764658 1105 18 -0.18814993642175892 1105 19 -0.4385509878539082 1105 20 -0.28965191171003823 1105 21 -0.2808918304330774 1105 22 -0.34188984811775924 1105 23 -0.464476043500774 1105 24 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1107 11 0.20361828590834108 1107 12 0.1504227102320428 1107 13 0.23430235102210162 1107 14 0.3693211370620446 1107 15 0.30932036844058786 1107 16 0.2919738596847452 1107 17 0.0745637048657386 1107 18 0.24470583813640445 1107 19 0.11066852094919 1107 20 0.3310376661136516 1107 21 0.1618437356950153 1107 22 0.42121385772936293 1107 23 0.2289229233213348 1107 24 0.4477563065510921 1107 25 0.3475884523455335 1108 1 0.6724740517029133 1108 2 0.5877501725506846 1108 3 0.7980052542742904 1108 4 0.8902438270268007 1108 5 0.8632871200882875 1108 6 0.8004258472838046 1108 7 0.7710421053952032 1108 8 0.7109530945138545 1108 9 0.6801152938305179 1108 10 0.7742565546520552 1108 11 0.9348208664362616 1108 12 0.958641977221923 1108 13 0.7477501725658726 1108 14 0.8404492093895186 1108 15 0.9081281074282401 1108 16 0.7786915398057204 1108 17 0.8046596958312975 1108 18 0.9343675008115611 1108 19 0.7099403136242799 1108 20 0.9109490091434296 1108 21 0.8618800811586524 1108 22 0.7571205796930585 1108 23 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0.06257624624100089 2214 14 0.3015054706284701 2214 15 0.18208715439482148 2214 16 0.34991745174869493 2214 17 0.2442399830936992 2214 18 0.06510264068120364 2214 19 -0.05276915472574473 2214 20 -0.06786269449128923 2214 21 -0.027377878663365607 2214 22 0.0603278390546933 2214 23 0.01816099113700176 2214 24 -0.29611135280749323 2214 25 -0.05238138298278017 2215 1 0.3949919109303437 2215 2 0.36340012315130465 2215 3 0.2628946822380378 2215 4 0.44776776508254557 2215 5 0.41444750719718826 2215 6 0.43573600988889893 2215 7 0.19165716323660575 2215 8 0.18884580532961337 2215 9 0.06717863607566699 2215 10 0.29416863230161283 2215 11 -0.22273325221905083 2215 12 0.0814058368081092 2215 13 0.20111118093956293 2215 14 0.19761952192955334 2215 15 -0.0320829859351619 2215 16 0.093216095709586 2215 17 0.3093248762457961 2215 18 0.3445303906324426 2215 19 -0.14959177319334316 2215 20 0.009850264485542937 2215 21 0.17817276464103315 2215 22 0.26232103866595224 2215 23 0.26768215406018925 2215 24 0.11317340337442516 2215 25 0.272704524126695 2216 1 -0.06176178228991347 2216 2 0.3628669933471613 2216 3 -1.9500477324510648 2216 4 -1.1769534153219325 2216 5 -0.1138679241558691 2216 6 0.0827019908598956 2216 7 0.043987499537799124 2216 8 0.2618886396356288 2216 9 0.05617898528379328 2216 10 -0.018281082943879312 2216 11 0.08706215719303856 2216 12 0.07338782382664277 2216 13 0.07023429584361907 2216 14 -0.02831813429246921 2216 15 -0.10084376702703679 2216 16 0.12503292592070636 2216 17 -0.10670191312175348 2216 18 0.24946718882336683 2216 19 0.20612085849903394 2216 20 0.33786202250942665 2216 21 0.19733870007723198 2216 22 0.2851524440625539 2216 23 0.13393139143339994 2216 24 0.0079159170733476 2216 25 0.051560985003639814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19077.0 3 23.0 4 297.0 5 2050.0 6 2767.0 7 2013.0 8 1595.0 9 1366.0 10 1144.0 11 1055.0 12 972.0 13 962.0 14 875.0 15 944.0 16 948.0 17 925.0 18 1044.0 19 1353.0 20 1683.0 21 2089.0 22 2675.0 23 3058.0 24 3969.0 25 4525.0 26 5229.0 27 5752.0 28 6711.0 29 8134.0 30 10317.0 31 13513.0 32 17148.0 33 22680.0 34 30602.0 35 42304.0 36 62444.0 37 107070.0 38 226859.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.18746557525675 21.212628336255204 11.446643475830784 27.153262612657258 2 13.83636150966937 22.31086894364733 39.87916536702017 23.973604179663134 3 17.92981430982989 26.554181275159074 30.132937280872614 25.383067134138425 4 12.148880507390794 19.21995806365755 38.707536207422606 29.923625221529054 5 12.772846563216655 39.033308826035515 35.120743074253866 13.073101536493963 6 29.392778743999116 39.177072544766446 18.687100619467298 12.743048091767143 7 26.473304796549613 33.45881077255469 22.239374593172272 17.82850983772342 8 24.56542697712466 37.59158088820072 20.562323695631708 17.280668439042916 9 25.651009543595404 17.064208271116016 20.86411737973122 36.42066480555736 10 14.640032589308175 30.63946779452506 33.80361695877485 20.916882657391913 11 32.287796826386085 24.656451505575816 24.72120919981238 18.33454246822572 12 22.67198987292468 26.554197705178765 31.39117451352711 19.382637908369443 13 27.57611117692978 23.541354591501385 26.23735943431288 22.645174797255958 14 20.760308420876672 23.63256303850717 28.095285237377286 27.511843303238877 15 22.327103228445115 31.30221959659043 24.164973350558633 22.205703824405827 16 21.088114098937464 30.04802269514596 27.710669347757992 21.153193858158584 17 20.686269418279313 29.838908616425286 28.097024856695857 21.377797108599548 18 20.525504325105086 29.033059058376747 29.845172598471205 20.596264018046966 19 22.15808026382415 28.184521104633802 28.71765492528012 20.93974370626193 20 22.316406186604347 28.34136683058247 29.01212327766688 20.330103705146307 21 22.106308830284725 27.754340800522016 28.532495337403198 21.606855031790065 22 22.120122303512655 28.573677479664767 28.362210551599226 20.94398966522335 23 21.536751221253876 28.49489588912151 28.43176396124446 21.536588928380155 24 21.592834468297813 28.225073518433163 29.228689391923034 20.953402621345987 25 21.738954017746284 28.172833935750468 28.72499103272204 21.363221013781207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 486.0 1 486.0 2 466.5 3 447.0 4 447.0 5 447.0 6 656.5 7 866.0 8 866.0 9 866.0 10 1415.5 11 1965.0 12 1965.0 13 1965.0 14 2987.0 15 4009.0 16 4009.0 17 4009.0 18 6778.5 19 9548.0 20 9548.0 21 9548.0 22 14601.0 23 19654.0 24 19654.0 25 19654.0 26 27025.5 27 34397.0 28 34397.0 29 34397.0 30 43550.0 31 52703.0 32 52703.0 33 52703.0 34 58975.5 35 65248.0 36 65248.0 37 65248.0 38 69043.5 39 72839.0 40 72839.0 41 72839.0 42 75171.0 43 77503.0 44 77503.0 45 77503.0 46 78582.5 47 79662.0 48 79662.0 49 79662.0 50 75298.0 51 70934.0 52 70934.0 53 70934.0 54 61429.5 55 51925.0 56 51925.0 57 51925.0 58 43373.0 59 34821.0 60 34821.0 61 34821.0 62 27761.5 63 20702.0 64 20702.0 65 20702.0 66 15720.0 67 10738.0 68 10738.0 69 10738.0 70 7791.0 71 4844.0 72 4844.0 73 4844.0 74 3358.5 75 1873.0 76 1873.0 77 1873.0 78 1284.0 79 695.0 80 695.0 81 695.0 82 464.5 83 234.0 84 234.0 85 234.0 86 149.0 87 64.0 88 64.0 89 64.0 90 38.5 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11344235051251925 2 0.10062125510409431 3 0.01493089591867206 4 0.0 5 0.004706478061320541 6 0.34016475919061556 7 0.02028654336776095 8 0.008114617347104381 9 0.008439202040988554 10 0.0030835545918996648 11 0.004706478061320541 12 3.245846938841752E-4 13 1.622923469420876E-4 14 1.622923469420876E-4 15 0.003895016326610102 16 8.11461734710438E-4 17 0.0 18 3.245846938841752E-4 19 6.491693877683504E-4 20 3.245846938841752E-4 21 0.01606694234726667 22 0.0 23 3.245846938841752E-4 24 0.0 25 0.006978570918509766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 616172.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.33990191841836 #Duplication Level Percentage of deduplicated Percentage of total 1 91.6538297763877 77.3007501378795 2 6.560854986272123 11.066837320863138 3 0.9700289477991515 2.45436438946221 4 0.2976271718234428 1.0040738591938163 5 0.1473575690755404 0.6214061461383817 6 0.08542385307328997 0.43227836338167946 7 0.05160508389884597 0.30466574001642943 8 0.03886029072553998 0.26219784866506113 9 0.02753512454901338 0.20900787333977003 >10 0.13990529064622964 2.151964850621764 >50 0.0136786880186741 0.7922857506095539 >100 0.01136666443642439 1.763615618487312 >500 0.0013485873058280541 0.7274467591049778 >1k 5.779659882120232E-4 0.9091053422363974 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2605 0.4227715637841382 No Hit GGTATCAACGCAGAGTACTTTTTTT 1670 0.2710282193932863 No Hit TATCAACGCAGAGTACTTTTTTTTT 1320 0.21422589796355565 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 837 0.13583869439052731 No Hit GTACATGGGAAGCAGTGGTATCAAC 756 0.12269301428821822 No Hit CCCATGTACTCTGCGTTGATACCAC 668 0.10841128775731451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATGT 25 0.006067297 18.978655 8 AATACCT 50 8.543816E-5 15.232371 5 GGCGAGG 190 0.0 12.985396 19 TAGGACC 190 0.0 12.025557 4 TCTAGAC 75 2.0263536E-4 11.42614 3 GAGGTGT 75 2.0294222E-4 11.424279 6 CTGGACT 75 2.0294222E-4 11.424279 4 TGGCGAG 410 0.0 11.340903 18 ATGGCGA 470 0.0 10.902633 17 TATGGCG 480 0.0 10.675494 16 GGACGTG 370 0.0 10.549448 6 AGGACGT 390 0.0 10.496667 5 CTGTAGG 400 0.0 10.482497 1 TGTAGGA 400 0.0 10.480789 2 TCAGGAC 100 2.3321929E-5 10.473961 3 GTACTGT 100 2.3363904E-5 10.472256 6 GACGTGA 265 0.0 10.386233 7 TTAGGAC 370 0.0 10.29382 3 CTCCTAC 75 0.0025764345 10.164846 1 CTAGACA 85 6.453381E-4 10.080247 4 >>END_MODULE