FastQCFastQC Report
Thu 26 May 2016
SRR1926821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196176
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC7380.37619280645950576No Hit
CCCATGTACTCTGCGTTGATACCAC6330.32266943968681183No Hit
GTATCAACGCAGAGTACTTTTTTTT5960.303808824728815No Hit
GTCCTACAGTGGACATTTCTAAATT4820.24569774080417583No Hit
GTCCTAAAGTGTGTATTTCTCATTT4390.22377864774488215No Hit
GGTATCAACGCAGAGTACTTTTTTT4390.22377864774488215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4280.21817143789250468No Hit
CTGTAGGACGTGGAATATGGCAAGA3620.18452817877823996No Hit
TATCAACGCAGAGTACTTTTTTTTT3400.17331375907348504No Hit
CTTTAGGACGTGAAATATGGCGAGG3320.16923578827175598No Hit
GAGTACATGGGAAGCAGTGGTATCA3150.16057010031808172No Hit
GTCCTACAGTGTGCATTTCTCATTT2500.12743658755403311No Hit
CATGTACTCTGCGTTGATACCACTG2480.12641709485360084No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2300.11724166054971047No Hit
GCGTTGATACCACTGCTTCCCATGT2080.10602724084495556No Hit
GTATCAACGCAGAGTACATGGGAAG2030.10347850909387488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTATA350.002114906216.3384342
TCCACCT601.4757152E-615.80761812
TTCCACC653.3927045E-614.59164711
TGGAATA2850.012.31330211
TTTCCAC853.965337E-612.27415110
TACATTG550.003019244212.1083285
CTGGAAT1750.011.9234610
GACGTGG802.8022945E-511.8890677
TAGGACC907.209177E-611.6337914
GTAGGAC2050.011.622873
ACCTGGA1800.011.6159528
CTGTAGG2150.011.5433131
ACATTTC2000.011.38148413
CCACCTT752.0875304E-411.38148413
TGTAGGA2350.011.3557922
TTACACT600.005760265511.1049824
GGACATG600.00579330211.0964616
GGAATAT3250.011.08965112
CACCTTT600.005915826711.06533214
ACGTGGA951.3371378E-511.0045868
Produced by FastQC (version 0.11.5)