##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926821_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 196176 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.51165280156594 31.0 30.0 33.0 22.0 34.0 2 28.590882676780033 31.0 30.0 33.0 20.0 34.0 3 28.37797182122176 31.0 28.0 33.0 16.0 34.0 4 31.880627599706386 35.0 32.0 37.0 19.0 37.0 5 32.149350583149825 35.0 33.0 37.0 20.0 37.0 6 31.32190482016149 35.0 32.0 37.0 17.0 37.0 7 31.400252834189708 35.0 32.0 37.0 17.0 37.0 8 31.20535131718457 35.0 32.0 37.0 13.0 37.0 9 33.31592039800995 37.0 33.0 39.0 17.0 39.0 10 33.50042308947068 38.0 34.0 39.0 17.0 39.0 11 33.639068999265966 38.0 34.0 39.0 19.0 39.0 12 33.5370330723432 38.0 34.0 39.0 17.0 39.0 13 33.63497063861023 38.0 35.0 39.0 18.0 39.0 14 34.690629842590326 39.0 35.0 41.0 18.0 41.0 15 34.27512539760215 39.0 34.0 41.0 16.0 41.0 16 34.351148968273385 39.0 34.0 41.0 17.0 41.0 17 34.59752467172335 39.0 35.0 41.0 17.0 41.0 18 34.63593405921213 39.0 35.0 41.0 17.0 41.0 19 34.015017127477364 39.0 34.0 41.0 10.0 41.0 20 34.29417461870973 39.0 34.0 41.0 12.0 41.0 21 34.27842345648805 39.0 34.0 41.0 11.0 41.0 22 34.32471352255118 39.0 34.0 41.0 10.0 41.0 23 34.29955754016801 39.0 35.0 41.0 2.0 41.0 24 33.0622655166789 39.0 33.0 41.0 2.0 41.0 25 33.64495656961096 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3647501664126658 1101 2 -0.20168429757954343 1101 3 -0.4357085421291309 1101 4 0.024026616978552795 1101 5 0.46864695656340416 1101 6 -1.404859597678275 1101 7 -0.3028825596791158 1101 8 0.17314063818285064 1101 9 0.33462276098731536 1101 10 -8.6088815300124 1101 11 -3.458297801034824 1101 12 -1.1448451990316926 1101 13 -0.21246940503612421 1101 14 -0.010561599633803098 1101 15 -2.032485991836431 1101 16 -0.1773610787949309 1101 17 0.24706632635363235 1101 18 0.6952058940921688 1101 19 0.00478475246762855 1101 20 0.6331464752551312 1101 21 0.23646192519627363 1101 22 0.3912623868958107 1101 23 0.5936601730137951 1101 24 0.42788321076133684 1101 25 0.7567453655495484 1102 1 -0.153112090173817 1102 2 -0.0337289711190607 1102 3 -0.2129493783261509 1102 4 -0.5315647346823873 1102 5 0.12142473434118273 1102 6 -1.349662003176558 1102 7 -0.19012482772035355 1102 8 0.09116755685409927 1102 9 -0.09868909468278275 1102 10 -0.18212036162751843 1102 11 -0.1638390381482253 1102 12 -0.5493769859732751 1102 13 -0.24525863756762334 1102 14 -0.6340581632076834 1102 15 0.0095744319894564 1102 16 -0.04251714981440813 1102 17 -0.17668796917901375 1102 18 -0.6126406122079473 1102 19 0.1347246150105832 1102 20 -0.6299906954207017 1102 21 -0.2602448331084233 1102 22 -0.5687891594959424 1102 23 -0.5689973298498998 1102 24 -3.4860271557907794 1102 25 -1.5471635691583572 1103 1 0.20738067999360865 1103 2 -0.01831801221495155 1103 3 0.11890944967993633 1103 4 0.05828496090360957 1103 5 -0.1261019018840841 1103 6 0.25621699225723304 1103 7 0.36438430469974037 1103 8 0.1236066740534909 1103 9 -0.1610464006188579 1103 10 -0.11220027030331892 1103 11 -0.2126404080112394 1103 12 -0.09175712295957794 1103 13 0.04260285406098774 1103 14 0.041455535422450396 1103 15 0.5004895765861122 1103 16 -0.09954721069721018 1103 17 -0.5131605719187391 1103 18 -0.330296624384502 1103 19 -1.9580265265419285 1103 20 -0.6958677882669733 1103 21 -0.556858227324561 1103 22 -0.44378915949594244 1103 23 -0.3585711502456377 1103 24 1.0593971212259632 1103 25 0.3612268418005442 1104 1 -0.06238739270407123 1104 2 -0.08777187540949072 1104 3 -1.4410503134196624 1104 4 -0.47088679188810545 1104 5 -0.1551154196742175 1104 6 -1.598218108787119 1104 7 -0.008812946532234633 1104 8 0.5046026503634522 1104 9 0.40450810003668636 1104 10 0.6827723776464083 1104 11 0.20765436119170744 1104 12 0.261163443069627 1104 13 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1.5514641991947649 1110 8 1.8582914353910205 1110 9 1.6085443421702195 1110 10 1.521425187026722 1110 11 1.3823566140256887 1110 12 1.2699164922875923 1110 13 1.4145929842405138 1110 14 1.1691250666059787 1110 15 1.7547650807127155 1110 16 1.3890089026355597 1110 17 0.9719921550445889 1110 18 1.1118245361702463 1110 19 -1.2696490909935605 1110 20 0.8466958125158044 1110 21 0.5559214884926789 1110 22 1.1338505920568522 1110 23 1.212921234677367 1110 24 -0.0955207720778759 1110 25 0.5976345674876029 1111 1 1.390778113120959 1111 2 1.1299145620039681 1111 3 1.8061204604400594 1111 4 1.4880308664392423 1111 5 1.8398351794866485 1111 6 2.4181481966131386 1111 7 1.8544992521955272 1111 8 2.50102121966518 1111 9 1.5014357738766293 1111 10 1.5734658633987664 1111 11 1.5134085959211774 1111 12 1.7922042443106392 1111 13 1.4894504405957107 1111 14 1.3897983036692878 1111 15 0.44180585505289827 1111 16 0.9922379325550281 1111 17 1.399613292650642 1111 18 1.4377449468526962 1111 19 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2213 18 -0.06731897478104543 2213 19 0.2137364829156354 2213 20 -1.3925788447155085 2213 21 -0.307759452991462 2213 22 -0.12490371057953098 2213 23 -0.4795580453641719 2213 24 -0.646835119327072 2213 25 -0.4993694515112992 2214 1 0.4462947825043315 2214 2 0.429416925537474 2214 3 -0.043485936921221935 2214 4 0.4712320400558774 2214 5 0.6895182838177121 2214 6 0.16445907078494315 2214 7 0.7487682827153144 2214 8 -2.041536766009795 2214 9 -0.7108028231528962 2214 10 -0.7846406250520417 2214 11 -0.37375545152208645 2214 12 -1.4637956789823932 2214 13 -0.30583783378511953 2214 14 -0.07079828478823202 2214 15 -1.3002765474093962 2214 16 -0.29157785565702454 2214 17 -0.1738384251060623 2214 18 0.0387440551341669 2214 19 0.9277210689112891 2214 20 -0.7034561631680845 2214 21 0.24351234352108975 2214 22 0.38599807454661317 2214 23 0.06794964752261023 2214 24 -1.358504781592874 2214 25 -0.40031706459908634 2215 1 0.24769496688323756 2215 2 -0.04011035250044159 2215 3 0.044276597649822946 2215 4 0.7121477277800743 2215 5 0.540719028635479 2215 6 1.1280031619886088 2215 7 0.7882910881955603 2215 8 -0.296782893596351 2215 9 0.14277111552742383 2215 10 -0.15152726853443 2215 11 -0.2038165156257037 2215 12 -0.3157996586459504 2215 13 0.12148310417411778 2215 14 0.13940279775327724 2215 15 0.34713073829576757 2215 16 0.006750868203610594 2215 17 -0.10255133254237592 2215 18 -0.9797577293250583 2215 19 0.0921195099054728 2215 20 -2.811390845570852 2215 21 -1.3920015898651812 2215 22 -0.28162699733378105 2215 23 -0.3888171496697197 2215 24 -0.4253101888238149 2215 25 -0.10703594153073226 2216 1 -0.7660536059707646 2216 2 -1.1794957442820007 2216 3 -0.5383731447443267 2216 4 -0.7201407907704223 2216 5 -0.8977002212853691 2216 6 -1.9264058796408818 2216 7 -10.473737713769342 2216 8 -10.45691107289386 2216 9 -5.518983290635926 2216 10 -3.2456853243538255 2216 11 -2.400460443899025 2216 12 -2.346002385334298 2216 13 -2.22018764005254 2216 14 -2.083538993878612 2216 15 -3.5410002041482755 2216 16 -2.0627403482524613 2216 17 -2.630561771095948 2216 18 -2.0326796608412394 2216 19 -1.212786034616684 2216 20 -2.1674529779908127 2216 21 -5.205354879256809 2216 22 -3.8655143991125556 2216 23 -3.5569277522851124 2216 24 -4.099767393632455 2216 25 -3.2266763487110737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10939.0 3 3.0 4 80.0 5 503.0 6 799.0 7 593.0 8 527.0 9 444.0 10 400.0 11 378.0 12 371.0 13 349.0 14 357.0 15 393.0 16 391.0 17 393.0 18 462.0 19 569.0 20 783.0 21 1005.0 22 1195.0 23 1398.0 24 1611.0 25 1841.0 26 2014.0 27 2161.0 28 2536.0 29 2953.0 30 3627.0 31 4435.0 32 5753.0 33 7665.0 34 10366.0 35 15086.0 36 22441.0 37 37011.0 38 54344.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.77503895374083 19.728217834427443 12.639914174052977 24.856829037778745 2 14.263255681006145 21.522489230078197 41.018599808081014 23.195655280834643 3 18.4788157223073 26.192955589586525 29.949974476773868 25.37825421133231 4 12.478765896882605 17.854501119732287 37.25367165062312 32.41306133276199 5 12.37744160790492 38.84342607746863 35.816367631837906 12.962764682788544 6 30.219906286168797 37.43964431573097 19.13689459488398 13.20355480321625 7 26.39882215212846 32.618820005009944 22.704244648818317 18.27811319404328 8 24.284509418048916 37.473355994581745 21.68732588749457 16.55480869987477 9 26.133507359576626 16.148241808105475 20.64658227073657 37.071668561581326 10 15.256069638294942 29.948648386784228 33.993034130719685 20.802247844201144 11 32.32047719799128 23.936883428076168 24.943791582757655 18.798847791174897 12 23.356547807200904 26.434419961048626 31.244710464867293 18.964321766883177 13 28.514183167812064 23.873299753247547 25.295695087382995 22.316821991557394 14 20.091259017563537 24.801549873817837 27.247699406051645 27.85949170256698 15 22.950134338021993 32.79020326591791 22.96389952433634 21.29576287172376 16 20.393804362234754 29.50116754529974 29.156002406468918 20.949025685996595 17 20.725396525968563 30.51937126862818 28.21184759943102 20.543384605972236 18 20.7382105531481 28.31098648993117 30.821820035686976 20.12898292123375 19 23.19529282196139 27.81375238367172 27.93510294402578 21.055851850341107 20 23.193045960896274 28.43253715363634 28.521247036631063 19.85316984883632 21 22.13039953913057 27.74953989528475 27.404397632436233 22.715662933148444 22 22.655409573627406 29.635521022927154 26.844700040276738 20.86436936316871 23 21.240842047302706 29.164733533529454 28.46931544144263 21.12510897772521 24 22.86538736056447 28.454024512103103 28.403042600485346 20.277545526847074 25 21.75325006372674 27.978078001529443 28.49349987254652 21.7751720621973 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 580.0 2 584.0 3 588.0 4 588.0 5 588.0 6 628.5 7 669.0 8 669.0 9 669.0 10 733.0 11 797.0 12 797.0 13 797.0 14 1052.5 15 1308.0 16 1308.0 17 1308.0 18 2099.5 19 2891.0 20 2891.0 21 2891.0 22 4335.5 23 5780.0 24 5780.0 25 5780.0 26 8166.0 27 10552.0 28 10552.0 29 10552.0 30 13544.5 31 16537.0 32 16537.0 33 16537.0 34 18400.5 35 20264.0 36 20264.0 37 20264.0 38 21020.5 39 21777.0 40 21777.0 41 21777.0 42 22400.0 43 23023.0 44 23023.0 45 23023.0 46 24344.5 47 25666.0 48 25666.0 49 25666.0 50 24039.5 51 22413.0 52 22413.0 53 22413.0 54 19318.5 55 16224.0 56 16224.0 57 16224.0 58 13943.5 59 11663.0 60 11663.0 61 11663.0 62 9635.5 63 7608.0 64 7608.0 65 7608.0 66 5944.0 67 4280.0 68 4280.0 69 4280.0 70 3230.0 71 2180.0 72 2180.0 73 2180.0 74 1544.5 75 909.0 76 909.0 77 909.0 78 625.5 79 342.0 80 342.0 81 342.0 82 219.5 83 97.0 84 97.0 85 97.0 86 58.0 87 19.0 88 19.0 89 19.0 90 12.0 91 5.0 92 5.0 93 5.0 94 3.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21970067694315307 2 0.13253405105619445 3 0.14068999265965254 4 0.07493271348177147 5 0.02293858575972596 6 0.023958078460158225 7 0.28800668787211486 8 0.27577277546692763 9 0.018860614957996902 10 0.039250468966642194 11 0.015802136856700105 12 0.0173313759073485 13 0.014272897806051708 14 0.015802136856700105 15 0.014782644156267842 16 0.01937036130821303 17 0.017841122257564634 18 0.013253405105619445 19 0.02548731751080662 20 0.015802136856700105 21 0.01274365875540331 22 0.016821629557132373 23 0.017841122257564634 24 0.014272897806051708 25 0.013253405105619445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 196176.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.38939003335864 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12975670392238 63.21010308222446 2 10.508928312779329 15.424876775583352 3 1.8375194911401895 4.0456330388755815 4 0.5213396086090276 1.5304318350418584 5 0.26579828606448574 0.975338704309239 6 0.1459493301422627 0.6426679388950766 7 0.10626628725400855 0.5459172601876953 8 0.06783301796183171 0.39825790498725505 9 0.049137501691614516 0.32455541492196455 >10 0.2918164796999641 4.372628953363438 >50 0.04519648199747242 2.437952550404409 >100 0.027510902085417992 4.673283194076005 >500 0.0029475966520090707 1.4183533471296872 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 738 0.37619280645950576 No Hit CCCATGTACTCTGCGTTGATACCAC 633 0.32266943968681183 No Hit GTATCAACGCAGAGTACTTTTTTTT 596 0.303808824728815 No Hit GTCCTACAGTGGACATTTCTAAATT 482 0.24569774080417583 No Hit GTCCTAAAGTGTGTATTTCTCATTT 439 0.22377864774488215 No Hit GGTATCAACGCAGAGTACTTTTTTT 439 0.22377864774488215 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 428 0.21817143789250468 No Hit CTGTAGGACGTGGAATATGGCAAGA 362 0.18452817877823996 No Hit TATCAACGCAGAGTACTTTTTTTTT 340 0.17331375907348504 No Hit CTTTAGGACGTGAAATATGGCGAGG 332 0.16923578827175598 No Hit GAGTACATGGGAAGCAGTGGTATCA 315 0.16057010031808172 No Hit GTCCTACAGTGTGCATTTCTCATTT 250 0.12743658755403311 No Hit CATGTACTCTGCGTTGATACCACTG 248 0.12641709485360084 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 230 0.11724166054971047 No Hit GCGTTGATACCACTGCTTCCCATGT 208 0.10602724084495556 No Hit GTATCAACGCAGAGTACATGGGAAG 203 0.10347850909387488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTATA 35 0.0021149062 16.338434 2 TCCACCT 60 1.4757152E-6 15.807618 12 TTCCACC 65 3.3927045E-6 14.591647 11 TGGAATA 285 0.0 12.313302 11 TTTCCAC 85 3.965337E-6 12.274151 10 TACATTG 55 0.0030192442 12.108328 5 CTGGAAT 175 0.0 11.92346 10 GACGTGG 80 2.8022945E-5 11.889067 7 TAGGACC 90 7.209177E-6 11.633791 4 GTAGGAC 205 0.0 11.62287 3 ACCTGGA 180 0.0 11.615952 8 CTGTAGG 215 0.0 11.543313 1 ACATTTC 200 0.0 11.381484 13 CCACCTT 75 2.0875304E-4 11.381484 13 TGTAGGA 235 0.0 11.355792 2 TTACACT 60 0.0057602655 11.104982 4 GGACATG 60 0.005793302 11.096461 6 GGAATAT 325 0.0 11.089651 12 CACCTTT 60 0.0059158267 11.065332 14 ACGTGGA 95 1.3371378E-5 11.004586 8 >>END_MODULE