FastQCFastQC Report
Thu 26 May 2016
SRR1926821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196176
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13370.6815308702389691No Hit
GGTATCAACGCAGAGTACTTTTTTT10030.5112755892667808No Hit
GTACATGGGAAGCAGTGGTATCAAC8750.4460280564391159No Hit
CCCATGTACTCTGCGTTGATACCAC8320.42410896337982223No Hit
TATCAACGCAGAGTACTTTTTTTTT7620.38842671886469293No Hit
GTCCTACAGTGGACATTTCTAAATT5010.25538292145828234No Hit
CTGTAGGACGTGGAATATGGCAAGA4500.22938585759725963No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4250.21664219884185631No Hit
CTTTAGGACGTGAAATATGGCGAGG4070.20746676453796592No Hit
GTCCTAAAGTGTGTATTTCTCATTT3980.2028790473860207No Hit
GAGTACATGGGAAGCAGTGGTATCA3410.17382350542370117No Hit
GTCCTACAGTGTGCATTTCTCATTT2760.14068999265965254No Hit
CATGTACTCTGCGTTGATACCACTG2570.13100481200554603No Hit
CTGAAGGACCTGGAATATGGCGAGA2370.12080988500122339No Hit
GTATCAACGCAGAGTACATGGGAAG2300.11724166054971047No Hit
GCGTTGATACCACTGCTTCCCATGT2280.1162221678492782No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2270.11571242149906207No Hit
ACGCAGAGTACATGGGAAGCAGTGG2230.11367343609819754No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2190.11163445069733301No Hit
GGTATCAACGCAGAGTACATGGGAA1980.10092977734279422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGAC402.6884658E-416.6709233
CCAGACA350.00212161816.33074
ACAGTGC350.00218411416.2599328
AGGACTT456.585754E-414.8185995
AACAGTG400.00531292514.2274417
CTAAATT1501.8189894E-1212.64983819
GGCGAGG1207.366907E-1012.649837519
TGTAGGA2050.012.0975482
GACGTGA1501.4551915E-1112.0142837
TAGGACA657.786145E-411.7246054
GTCCTAA1104.6191053E-711.2756051
TCTAAAT1604.5474735E-1111.26626218
CTGTAGG2150.011.0940681
TGGCGAG3100.011.01760218
GTCCTAC2600.011.0087281
GGACGTG2250.011.0081016
TTAGGAC1751.8189894E-1110.8871343
AGGACGT2450.010.8871345
GTAGGAC2300.010.354613
TCCTACA2600.010.2721792
Produced by FastQC (version 0.11.5)