##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926821_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 196176 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.69012519370361 31.0 30.0 33.0 25.0 34.0 2 29.987037150314002 31.0 30.0 34.0 26.0 34.0 3 30.055832517739173 31.0 30.0 34.0 26.0 34.0 4 33.62209954326727 35.0 35.0 37.0 28.0 37.0 5 33.571471535763806 37.0 35.0 37.0 28.0 37.0 6 33.52279585678166 37.0 35.0 37.0 28.0 37.0 7 33.467600521980266 37.0 35.0 37.0 28.0 37.0 8 33.46900742190686 37.0 35.0 37.0 28.0 37.0 9 34.97249918440584 39.0 35.0 39.0 28.0 39.0 10 34.86637509175434 39.0 35.0 39.0 28.0 39.0 11 34.890669602805644 39.0 35.0 39.0 27.0 39.0 12 34.805098482994865 39.0 35.0 39.0 27.0 39.0 13 34.81072608270125 39.0 35.0 39.0 27.0 39.0 14 35.87742129516353 40.0 36.0 41.0 27.0 41.0 15 35.842962441888915 40.0 36.0 41.0 27.0 41.0 16 35.816710504852786 40.0 36.0 41.0 27.0 41.0 17 35.80875846178942 40.0 36.0 41.0 27.0 41.0 18 35.797171927249 40.0 36.0 41.0 27.0 41.0 19 35.83013722371748 40.0 36.0 41.0 27.0 41.0 20 35.80351317184569 40.0 36.0 41.0 27.0 41.0 21 35.769676209118344 40.0 36.0 41.0 27.0 41.0 22 35.6752253078868 40.0 36.0 41.0 27.0 41.0 23 35.592952246961914 40.0 36.0 41.0 27.0 41.0 24 35.52046631596118 40.0 36.0 41.0 27.0 41.0 25 35.41552483484218 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13672841239054634 1101 2 0.03156032961747712 1101 3 0.4437125934794288 1101 4 0.29802388493811804 1101 5 -0.10240291200967988 1101 6 -0.15858970903202874 1101 7 -0.1919759953157083 1101 8 -0.08673412600141717 1101 9 -0.22238007279803185 1101 10 0.0942448460004286 1101 11 -0.40649525840545664 1101 12 0.16566178629113892 1101 13 -0.06499882345266883 1101 14 -0.15444277764919434 1101 15 -0.2492158658949606 1101 16 0.2645661841604863 1101 17 -6.408808023863344E-4 1101 18 -0.0738759677860088 1101 19 -0.1063984412926331 1101 20 -0.07476160021349187 1101 21 -0.019429073671034303 1101 22 -0.08217352211293161 1101 23 0.0948639922690333 1101 24 -0.010451049909072196 1101 25 -0.3397951267884025 1102 1 -0.3193855624089963 1102 2 -0.2213749510239893 1102 3 -0.4707347146534495 1102 4 -0.2306129993688728 1102 5 -0.3473914572559522 1102 6 -0.9150688041064825 1102 7 -0.44559140195946867 1102 8 -0.4159867033210034 1102 9 -0.5196667280099447 1102 10 -0.41212686075787275 1102 11 -0.12639073377773258 1102 12 -0.15729067648090478 1102 13 -0.657316979237315 1102 14 -0.6463528578324684 1102 15 -0.4506405508892328 1102 16 -1.119705007133902 1102 17 -1.0052943745022702 1102 18 -0.9578249941319399 1102 19 -0.7060547986809524 1102 20 -0.889409366536519 1102 21 -0.7331318228119272 1102 22 -0.6478593754920823 1102 23 -0.9212442551536455 1102 24 -0.7161712675493916 1102 25 -0.9214460431687002 1103 1 -0.10601037215024434 1103 2 -0.359027158025512 1103 3 0.14047152583361466 1103 4 0.09967619393402316 1103 5 -0.01158476014789045 1103 6 0.16888287017053472 1103 7 0.1767925402018733 1103 8 0.45986946106256 1103 9 0.0454493298287133 1103 10 0.32693326100772424 1103 11 0.0505023408036962 1103 12 0.1930708151477063 1103 13 0.4151240405495926 1103 14 0.5449523172055848 1103 15 0.4539922497195903 1103 16 0.5484361648600142 1103 17 0.3524872084505475 1103 18 0.8103447166747557 1103 19 0.8704292195838903 1103 20 0.5629308161118871 1103 21 0.6558521954529155 1103 22 0.5764081283130906 1103 23 0.6675114526089061 1103 24 0.7238496609133165 1103 25 0.9233503799667631 1104 1 0.32300992713996024 1104 2 0.4420601424853636 1104 3 0.4946805768757052 1104 4 0.3606961315442234 1104 5 0.3999957814679149 1104 6 0.3247895567295984 1104 7 0.6105485870394318 1104 8 0.3828994352928561 1104 9 0.2247295866215211 1104 10 0.633321434071604 1104 11 0.5054505753531799 1104 12 0.41002230481722535 1104 13 0.460910468507457 1104 14 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2214 17 0.44710768561255776 2214 18 -0.34040267059259577 2214 19 -0.24658164771843616 2214 20 0.20133278138648336 2214 21 -0.14124985725974426 2214 22 0.08081125942855039 2214 23 0.18634186440055345 2214 24 -0.0019383225983631291 2214 25 0.39534938067459535 2215 1 -0.03428796481139784 2215 2 -0.07103711318615069 2215 3 -0.1777542341729692 2215 4 -0.033916302672174936 2215 5 0.10718812232362751 2215 6 -0.0350950803827601 2215 7 -0.07012218274024917 2215 8 0.1070988822645802 2215 9 -0.28646477605799703 2215 10 0.03926202813101298 2215 11 -0.22746055359755246 2215 12 -0.06813902482925727 2215 13 0.12186845619812203 2215 14 -0.2764204254000333 2215 15 0.009475815477131277 2215 16 -0.3198739260528214 2215 17 0.0016500699421726495 2215 18 -0.22978445359139954 2215 19 -0.23308182570797698 2215 20 -0.2799874446145978 2215 21 -0.05051170269180716 2215 22 -0.7162715376542437 2215 23 -0.17556107197046344 2215 24 -0.12016901529713664 2215 25 -0.023829676802336053 2216 1 -0.5161596128172299 2216 2 -0.2340435701223207 2216 3 -2.0675353891298407 2216 4 -1.7345178626986524 2216 5 -1.3566655076641894 2216 6 -0.9008138342804273 2216 7 -0.8310524420730658 2216 8 -0.5839180132251585 2216 9 -1.009804729429895 2216 10 -0.9799073009424717 2216 11 -0.7044103468412786 2216 12 -0.3005503498923403 2216 13 -0.6965985731315314 2216 14 -0.49976517590133795 2216 15 -0.5162799722594897 2216 16 -0.7367732534952793 2216 17 -0.6572068700649254 2216 18 -0.6322348272877818 2216 19 -0.49891954841471176 2216 20 -0.4468507935865702 2216 21 -0.4251579143987172 2216 22 -0.5111558788644643 2216 23 -0.5898411667617438 2216 24 -0.441288857183757 2216 25 -0.3064189753660784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5720.0 3 6.0 4 107.0 5 657.0 6 854.0 7 663.0 8 555.0 9 409.0 10 347.0 11 334.0 12 281.0 13 285.0 14 287.0 15 278.0 16 279.0 17 314.0 18 353.0 19 398.0 20 534.0 21 692.0 22 848.0 23 972.0 24 1172.0 25 1348.0 26 1588.0 27 1610.0 28 1944.0 29 2415.0 30 3009.0 31 3848.0 32 4905.0 33 6594.0 34 9183.0 35 12535.0 36 18746.0 37 33126.0 38 78980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80215764924421 20.1736121742858 10.807128203557978 27.217101972912012 2 14.258229140086756 21.82597601428936 39.570808879816276 24.344985965807602 3 18.28661452650685 26.034220803719833 29.702556363376807 25.976608306396514 4 12.29966968436506 18.41662588695865 36.99993883043797 32.28376559823832 5 12.686323688342407 39.096368344854305 34.889174364307415 13.32813360249587 6 30.826486868087454 37.978698370149075 17.790237264551507 13.404577497211962 7 27.004965891362204 32.838613629179456 22.014092118814304 18.142328360644036 8 24.844888324691944 38.11082391447318 20.03069095432554 17.013596806509337 9 26.393201086928812 15.586110558809885 20.28865811194551 37.73203024231579 10 15.558410309740827 29.94076385065557 33.31243245447686 21.18839338512673 11 32.56748145693676 23.54395534371575 24.613972930950986 19.2745902683965 12 23.742067031986746 26.300497005224926 30.91219574359628 19.045240219192046 13 29.192812539824136 23.082961641391613 25.393143876640757 22.331081942143495 14 20.577440665524836 24.50452654758992 27.18834108147786 27.72969170540739 15 22.990263546923586 32.094102054340624 23.380741193862466 21.534893204873324 16 20.589779533579712 28.90990187332739 29.43392379253218 21.066394800560722 17 20.816001957425982 30.14283092733056 28.42396623440176 20.617200880841693 18 20.957308525551166 27.403593730087934 31.26800050974895 20.371097234611955 19 22.932468803523367 27.235747492047956 28.580458363918115 21.25132534051056 20 23.135975532050466 27.618707786415193 29.306996304320123 19.93832037721422 21 21.707765955819752 26.765094238622282 28.582571590254446 22.944568215303516 22 22.854987358290515 28.825646358372076 27.565553380637798 20.753812902699618 23 21.03927085882065 28.550383329255364 29.17380311556969 21.236542696354295 24 22.764252507952044 27.549751243781095 29.527567082619687 20.158429165647174 25 21.798597092228952 27.675822271160865 28.963520319732467 21.562060316877716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 231.0 1 231.0 2 225.5 3 220.0 4 220.0 5 220.0 6 349.5 7 479.0 8 479.0 9 479.0 10 589.0 11 699.0 12 699.0 13 699.0 14 862.0 15 1025.0 16 1025.0 17 1025.0 18 1846.0 19 2667.0 20 2667.0 21 2667.0 22 4167.0 23 5667.0 24 5667.0 25 5667.0 26 8106.5 27 10546.0 28 10546.0 29 10546.0 30 13611.0 31 16676.0 32 16676.0 33 16676.0 34 18483.5 35 20291.0 36 20291.0 37 20291.0 38 20875.5 39 21460.0 40 21460.0 41 21460.0 42 22203.5 43 22947.0 44 22947.0 45 22947.0 46 24434.0 47 25921.0 48 25921.0 49 25921.0 50 24407.0 51 22893.0 52 22893.0 53 22893.0 54 19731.0 55 16569.0 56 16569.0 57 16569.0 58 14156.0 59 11743.0 60 11743.0 61 11743.0 62 9844.5 63 7946.0 64 7946.0 65 7946.0 66 6279.5 67 4613.0 68 4613.0 69 4613.0 70 3358.5 71 2104.0 72 2104.0 73 2104.0 74 1535.0 75 966.0 76 966.0 77 966.0 78 662.0 79 358.0 80 358.0 81 358.0 82 235.0 83 112.0 84 112.0 85 112.0 86 76.0 87 40.0 88 40.0 89 40.0 90 21.5 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11316368974798141 2 0.11520267514884594 3 0.01937036130821303 4 0.0 5 0.006116956202593589 6 0.35580295245086047 7 0.01937036130821303 8 0.013763151455835575 9 0.013763151455835575 10 0.006116956202593589 11 0.005607209852377457 12 5.097463502161325E-4 13 5.097463502161325E-4 14 0.0 15 0.0030584781012967946 16 5.097463502161325E-4 17 0.0 18 5.097463502161325E-4 19 0.0 20 5.097463502161325E-4 21 0.01274365875540331 22 0.0 23 0.0 24 0.0 25 0.006116956202593589 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 196176.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.8930442624733 #Duplication Level Percentage of deduplicated Percentage of total 1 83.79407514103315 58.56623002765243 2 12.229978178615758 17.095808123341474 3 2.1732200841414473 4.556789025989824 4 0.6756075923259812 1.8888108541801132 5 0.31939757434050237 1.1161834400353672 6 0.18802889044606516 0.7885146937542347 7 0.105161685321701 0.5145049228834156 8 0.072684088360838 0.4064089763985258 9 0.04320773657352756 0.2717928220333364 >10 0.31223516424436837 4.075680403179235 >50 0.04516565649372554 2.2714984578698596 >100 0.03534703551682868 4.803773173742906 >500 0.003927448390758742 2.0381723322880614 >1k 0.001963724195379371 1.6058327466512 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1337 0.6815308702389691 No Hit GGTATCAACGCAGAGTACTTTTTTT 1003 0.5112755892667808 No Hit GTACATGGGAAGCAGTGGTATCAAC 875 0.4460280564391159 No Hit CCCATGTACTCTGCGTTGATACCAC 832 0.42410896337982223 No Hit TATCAACGCAGAGTACTTTTTTTTT 762 0.38842671886469293 No Hit GTCCTACAGTGGACATTTCTAAATT 501 0.25538292145828234 No Hit CTGTAGGACGTGGAATATGGCAAGA 450 0.22938585759725963 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 425 0.21664219884185631 No Hit CTTTAGGACGTGAAATATGGCGAGG 407 0.20746676453796592 No Hit GTCCTAAAGTGTGTATTTCTCATTT 398 0.2028790473860207 No Hit GAGTACATGGGAAGCAGTGGTATCA 341 0.17382350542370117 No Hit GTCCTACAGTGTGCATTTCTCATTT 276 0.14068999265965254 No Hit CATGTACTCTGCGTTGATACCACTG 257 0.13100481200554603 No Hit CTGAAGGACCTGGAATATGGCGAGA 237 0.12080988500122339 No Hit GTATCAACGCAGAGTACATGGGAAG 230 0.11724166054971047 No Hit GCGTTGATACCACTGCTTCCCATGT 228 0.1162221678492782 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 227 0.11571242149906207 No Hit ACGCAGAGTACATGGGAAGCAGTGG 223 0.11367343609819754 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 219 0.11163445069733301 No Hit GGTATCAACGCAGAGTACATGGGAA 198 0.10092977734279422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 5.097463502161324E-4 0.0 12 0.0 0.0 0.0 5.097463502161324E-4 0.0 13 0.0 0.0 0.0 5.097463502161324E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAGAC 40 2.6884658E-4 16.670923 3 CCAGACA 35 0.002121618 16.3307 4 ACAGTGC 35 0.002184114 16.259932 8 AGGACTT 45 6.585754E-4 14.818599 5 AACAGTG 40 0.005312925 14.227441 7 CTAAATT 150 1.8189894E-12 12.649838 19 GGCGAGG 120 7.366907E-10 12.6498375 19 TGTAGGA 205 0.0 12.097548 2 GACGTGA 150 1.4551915E-11 12.014283 7 TAGGACA 65 7.786145E-4 11.724605 4 GTCCTAA 110 4.6191053E-7 11.275605 1 TCTAAAT 160 4.5474735E-11 11.266262 18 CTGTAGG 215 0.0 11.094068 1 TGGCGAG 310 0.0 11.017602 18 GTCCTAC 260 0.0 11.008728 1 GGACGTG 225 0.0 11.008101 6 TTAGGAC 175 1.8189894E-11 10.887134 3 AGGACGT 245 0.0 10.887134 5 GTAGGAC 230 0.0 10.35461 3 TCCTACA 260 0.0 10.272179 2 >>END_MODULE