FastQCFastQC Report
Thu 26 May 2016
SRR1926820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993090
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13530.13624142826934113No Hit
GTATCAACGCAGAGTACTTTTTTTT13200.13291846660423529No Hit
CTGTAGGACGTGGAATATGGCAAGA10790.10865077686815898No Hit
GTCCTAAAGTGTGTATTTCTCATTT10560.10633477328338822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2950.014.2006044
ATTAGAC759.314135E-713.9787723
GCCTCGA852.755187E-713.38773116
AGGACGT4900.013.2086135
GGACGTG4900.012.8201266
CTAGTAC751.428657E-512.7079753
TCCAACG604.1520534E-412.64396918
TTAGGAC3400.012.334213
TGTAGGA6800.012.1946672
GTAGGAC6800.012.1940493
TGCGCCA701.1090601E-412.19178312
GACGTGA2750.012.1137827
TAGGACG5250.011.7877314
TTAGACA1251.7695129E-811.4250314
TAGACAG1501.6916601E-1011.4215665
AATGCCG752.0526178E-411.4117588
GACGTGG2450.011.26617057
GTCCTAC6950.011.25715351
GTAATAC855.14459E-511.2129183
CGGTTTC855.4161792E-511.15644213
Produced by FastQC (version 0.11.5)