##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926820_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 993090 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.641758551591497 31.0 30.0 33.0 23.0 34.0 2 28.702721807691145 31.0 30.0 31.0 23.0 34.0 3 28.50396540092036 31.0 28.0 33.0 16.0 34.0 4 32.08880866789516 35.0 32.0 37.0 22.0 37.0 5 32.34128125346142 35.0 33.0 37.0 24.0 37.0 6 31.53031648692465 35.0 32.0 37.0 17.0 37.0 7 31.57591054184414 35.0 32.0 37.0 17.0 37.0 8 31.403623035173045 35.0 32.0 37.0 17.0 37.0 9 33.617317665065606 37.0 34.0 39.0 21.0 39.0 10 33.80454037398423 38.0 34.0 39.0 23.0 39.0 11 33.96351992266562 38.0 35.0 39.0 25.0 39.0 12 33.83581447804328 38.0 34.0 39.0 23.0 39.0 13 33.93681539437513 38.0 35.0 39.0 24.0 39.0 14 35.00426950226062 39.0 35.0 41.0 24.0 41.0 15 34.57430041587369 39.0 34.0 41.0 18.0 41.0 16 34.67060689363502 39.0 34.0 41.0 19.0 41.0 17 34.902269683513076 39.0 35.0 41.0 24.0 41.0 18 34.96018185662931 39.0 36.0 41.0 24.0 41.0 19 34.35563846177084 39.0 34.0 41.0 18.0 41.0 20 34.622977776435164 39.0 34.0 41.0 18.0 41.0 21 34.60714537453806 39.0 34.0 41.0 18.0 41.0 22 34.63428893655157 39.0 35.0 41.0 18.0 41.0 23 34.64366472323757 39.0 35.0 41.0 18.0 41.0 24 33.37058574751533 39.0 33.0 41.0 2.0 41.0 25 33.94275745400719 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.11010548262262887 1101 2 -0.2405165699556555 1101 3 -0.8916996722131785 1101 4 -0.49246214172479696 1101 5 -0.28328179296621414 1101 6 -2.5120887223835986 1101 7 -0.5239763545994229 1101 8 -0.20883706434458205 1101 9 -0.1448071549359824 1101 10 -8.860372338803543 1101 11 -3.943152458038778 1101 12 -1.5660077370790262 1101 13 -0.580182819753702 1101 14 -0.44442356007265005 1101 15 -1.8770218095506621 1101 16 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21 -0.620810210652273 2214 22 -0.3073320765610603 2214 23 -0.6586581861205616 2214 24 -2.026709045277638 2214 25 -1.4501453607904367 2215 1 0.4679522820922912 2215 2 0.19713470943576894 2215 3 0.4211341029520277 2215 4 0.7451113368367501 2215 5 0.5894658249836837 2215 6 1.55268527100813 2215 7 1.0673387675766968 2215 8 -0.13064963157986753 2215 9 0.2150769122224645 2215 10 0.11020982271482183 2215 11 0.038516321736850045 2215 12 -0.3556141225101044 2215 13 -0.04627462473295196 2215 14 0.14735980783883207 2215 15 0.09543333490914563 2215 16 0.3735276127673899 2215 17 0.08778825064962348 2215 18 -0.9179272285671445 2215 19 0.3166235243839637 2215 20 -3.8914263245113645 2215 21 -2.1038121878051754 2215 22 -1.0680904733585592 2215 23 -0.7896018494675658 2215 24 -0.7269690042700248 2215 25 -0.47777261458201536 2216 1 -0.5612054814404708 2216 2 -1.1595871566610967 2216 3 -0.3520051420622181 2216 4 -0.3646554010549856 2216 5 -0.650117508349652 2216 6 -1.5163941449462826 2216 7 -10.562397699659773 2216 8 -10.914989019044256 2216 9 -5.895758201737653 2216 10 -3.5862734393934375 2216 11 -2.463887524417 2216 12 -2.1587658319118113 2216 13 -1.8564687130520383 2216 14 -1.9503520867480617 2216 15 -2.9663989300481184 2216 16 -2.052211347346571 2216 17 -2.239891592655219 2216 18 -2.04073705762697 2216 19 -1.5644662192057766 2216 20 -2.3743857262207655 2216 21 -4.665502002619991 2216 22 -3.4408646898827726 2216 23 -3.290349712715429 2216 24 -3.383468292019309 2216 25 -2.849887999197403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48805.0 3 4.0 4 399.0 5 2358.0 6 3049.0 7 2571.0 8 2048.0 9 1815.0 10 1762.0 11 1757.0 12 1670.0 13 1649.0 14 1658.0 15 1614.0 16 1754.0 17 2010.0 18 2228.0 19 2841.0 20 3665.0 21 4761.0 22 5904.0 23 7266.0 24 8372.0 25 9631.0 26 11047.0 27 12249.0 28 13701.0 29 16196.0 30 19368.0 31 23559.0 32 30863.0 33 40684.0 34 55504.0 35 78238.0 36 114853.0 37 186488.0 38 270749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.262903121618784 18.710031006249697 12.271692262971754 25.755373609159765 2 14.342397283134236 20.362275657411132 41.57326936437401 23.722057695080622 3 18.675790384400138 24.55326756754304 29.895401940705916 26.875540107350904 4 11.889714269591819 16.684535733431144 38.92950854692242 32.49624145005461 5 12.558982752108774 37.94888581140627 36.746317512274956 12.745813924209996 6 31.11264870700942 37.52855295439987 19.052122486635 12.306675851955701 7 27.41024708568196 31.436450693981456 22.833606077135986 18.3196961432006 8 24.35784121109395 37.23606816696482 21.583141494634745 16.822949127306483 9 25.820840656048098 15.762980718389791 20.417054833495314 37.99912379206679 10 13.864240521948826 29.65567051224841 35.358870037602955 21.121218928199802 11 34.16530958157466 23.063094637424125 24.440926262189848 18.330669518811366 12 22.578832344676865 25.304502835043756 32.10498222432599 20.01168259595339 13 28.283367440661305 22.460581865643984 25.987568281796353 23.268482411898354 14 20.249424177205373 22.443946030272112 28.20872027853004 29.09790951399247 15 22.86607442290475 31.088164826521457 23.45794891368824 22.587811836885553 16 21.623488912903575 28.93270393821803 27.87530805882904 21.568499090049357 17 21.2563159734077 29.144874858875696 28.107025850044167 21.49178331767244 18 21.072036787313774 28.53705771986734 29.48624856614274 20.90465692667614 19 23.02589039136732 27.755373772004216 27.616182451419007 21.60255338520946 20 22.84470343207852 27.89079951577459 28.334639237969395 20.929857814177492 21 22.517730139323344 27.477335030646298 27.487506823157847 22.517428006872507 22 22.22263626100154 28.47232783008654 27.89121880819273 21.413817100719186 23 21.776534309845083 28.247133208852254 27.85989231782557 22.11644016347709 24 22.57853673372993 27.581845080734745 28.36839576894142 21.471222416593903 25 22.096938092116066 27.80189799414468 28.12326588736715 21.97789802637211 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 390.0 1 390.0 2 494.5 3 599.0 4 599.0 5 599.0 6 847.0 7 1095.0 8 1095.0 9 1095.0 10 1689.0 11 2283.0 12 2283.0 13 2283.0 14 3745.0 15 5207.0 16 5207.0 17 5207.0 18 9071.5 19 12936.0 20 12936.0 21 12936.0 22 20045.0 23 27154.0 24 27154.0 25 27154.0 26 38035.5 27 48917.0 28 48917.0 29 48917.0 30 63009.0 31 77101.0 32 77101.0 33 77101.0 34 87878.5 35 98656.0 36 98656.0 37 98656.0 38 107334.0 39 116012.0 40 116012.0 41 116012.0 42 121088.5 43 126165.0 44 126165.0 45 126165.0 46 128648.5 47 131132.0 48 131132.0 49 131132.0 50 124708.0 51 118284.0 52 118284.0 53 118284.0 54 104157.0 55 90030.0 56 90030.0 57 90030.0 58 76665.0 59 63300.0 60 63300.0 61 63300.0 62 50822.0 63 38344.0 64 38344.0 65 38344.0 66 29518.0 67 20692.0 68 20692.0 69 20692.0 70 15010.0 71 9328.0 72 9328.0 73 9328.0 74 6511.0 75 3694.0 76 3694.0 77 3694.0 78 2490.5 79 1287.0 80 1287.0 81 1287.0 82 821.5 83 356.0 84 356.0 85 356.0 86 224.5 87 93.0 88 93.0 89 93.0 90 58.5 91 24.0 92 24.0 93 24.0 94 14.0 95 4.0 96 4.0 97 4.0 98 5.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23381566625381386 2 0.1368456031175422 3 0.13986647735854757 4 0.06907732431098892 5 0.021649598727204986 6 0.01993776999063529 7 0.3171917953055614 8 0.31306326717618743 9 0.017621766405864523 10 0.03514283700369554 11 0.01540645862912727 12 0.01611132928536185 13 0.014701587972892687 14 0.016413416709462385 15 0.014399500548792153 16 0.018024549637998568 17 0.017218983173730475 18 0.013996717316658108 19 0.02134751130310445 20 0.016514112517495898 21 0.014902979588959711 22 0.016212025093395362 23 0.016916895749629943 24 0.0148022837809262 25 0.0148022837809262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 993090.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.6788660897008 #Duplication Level Percentage of deduplicated Percentage of total 1 92.074652860015 78.88851852648911 2 6.054966865136775 10.375653904312584 3 1.036427347018937 2.6639975963081803 4 0.3139562918707734 1.0759767635686006 5 0.1596721929205024 0.6840266217742298 6 0.09115944379457926 0.46862626666124174 7 0.05452360375955886 0.3270064381670205 8 0.0352354227345315 0.24151448528687386 9 0.02749400790264279 0.21200898792237363 >10 0.1322496443412606 1.983264886131271 >50 0.009541021541379448 0.5629672378235208 >100 0.00882671519540151 1.484795685578071 >500 8.238260345901557E-4 0.5468280539218761 >1k 4.707577340515176E-4 0.4848145460550212 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1353 0.13624142826934113 No Hit GTATCAACGCAGAGTACTTTTTTTT 1320 0.13291846660423529 No Hit CTGTAGGACGTGGAATATGGCAAGA 1079 0.10865077686815898 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1056 0.10633477328338822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 1.0069580803351156E-4 0.0 0.0 0.0 13 0.0 1.0069580803351156E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 295 0.0 14.200604 4 ATTAGAC 75 9.314135E-7 13.978772 3 GCCTCGA 85 2.755187E-7 13.387731 16 AGGACGT 490 0.0 13.208613 5 GGACGTG 490 0.0 12.820126 6 CTAGTAC 75 1.428657E-5 12.707975 3 TCCAACG 60 4.1520534E-4 12.643969 18 TTAGGAC 340 0.0 12.33421 3 TGTAGGA 680 0.0 12.194667 2 GTAGGAC 680 0.0 12.194049 3 TGCGCCA 70 1.1090601E-4 12.191783 12 GACGTGA 275 0.0 12.113782 7 TAGGACG 525 0.0 11.787731 4 TTAGACA 125 1.7695129E-8 11.425031 4 TAGACAG 150 1.6916601E-10 11.421566 5 AATGCCG 75 2.0526178E-4 11.411758 8 GACGTGG 245 0.0 11.2661705 7 GTCCTAC 695 0.0 11.2571535 1 GTAATAC 85 5.14459E-5 11.212918 3 CGGTTTC 85 5.4161792E-5 11.156442 13 >>END_MODULE