FastQCFastQC Report
Thu 26 May 2016
SRR1926820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993090
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22580.22737113453966912No Hit
GGTATCAACGCAGAGTACTTTTTTT13350.13442890372473795No Hit
CTGTAGGACGTGGAATATGGCAAGA12660.12748089297042564No Hit
TATCAACGCAGAGTACTTTTTTTTT12180.12264749418481709No Hit
GTCCTACAGTGGACATTTCTAAATT12110.12194262352858251No Hit
GTACATGGGAAGCAGTGGTATCAAC10300.1037166822745169No Hit
GTCCTAAAGTGTGTATTTCTCATTT10050.10119928707367912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAACG250.006069490718.97885117
ACGAAGC400.00531468414.2348568
AGGGCGT551.9767784E-413.80349610
AATCCCG604.1283114E-412.65256919
TAGGGCA951.0135518E-612.024184
CAAGACA1354.5693014E-911.2819484
ATTAATC855.2101655E-511.1989933
GGCGAGG2900.011.12553419
TGTAGGA7450.010.9996532
GTCCTAT1303.110108E-810.9964021
TGGCGAG6900.010.86470518
GTCCTAC6850.010.8519231
TAGGACC3350.010.7978344
GTATAAG1605.12955E-1010.7214921
ATAGGAC803.6986227E-410.7090363
GTAGGGC803.6986227E-410.7090363
CCATAAC803.6986227E-410.7090363
CTGTAGG7350.010.6323491
TATGGCG7200.010.54380616
TGGACTG1909.094947E-1210.5211585
Produced by FastQC (version 0.11.5)