FastQCFastQC Report
Thu 26 May 2016
SRR1926819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558873
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10340.1850152002333267No Hit
GTCCTACAGTGGACATTTCTAAATT10290.18412054259196634No Hit
GTCCTAAAGTGTGTATTTCTCATTT8860.15853333404905945No Hit
CTGTAGGACGTGGAATATGGCAAGA8250.14761851082446278No Hit
CTTTAGGACGTGAAATATGGCGAGG6170.11040075294387096No Hit
GTCCTACAGTGTGCATTTCTCATTT5990.10717998543497359No Hit
GTACATGGGAAGCAGTGGTATCAAC5980.10700105390670152No Hit
TATCAACGCAGAGTACTTTTTTTTT5870.10503280709570868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA350.002195692616.25463917
GTGCTAC551.0772066E-515.6178511
TATGTCG400.005340744714.22280916
TGCCGGT551.9870617E-413.7930510
TAGGACC2150.013.7268324
GTCCTAT706.9030393E-613.6346311
GTCCAAA500.001445902713.3619381
TCCTAAT655.25232E-513.2032072
GTTCTAA751.4057414E-512.72565651
GCCGGTT604.1510758E-412.64249711
TTAGGAC2550.012.3391423
GTCCTAA2900.012.1771361
AGGCTTA550.003021872812.1133816
CTAGGCT657.867612E-411.7171974
TAGAACT907.2624225E-611.6358284
TAGGCTT752.0321777E-411.42221455
GTATAGT600.005668111611.134951
TGTAGGA4800.011.1249262
AGTGCAA600.005947714711.06317610
TTACAAT1201.3024874E-711.06218416
Produced by FastQC (version 0.11.5)