##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926819_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 558873 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.609938214943288 31.0 30.0 33.0 23.0 34.0 2 28.675003086568864 31.0 30.0 33.0 22.0 34.0 3 28.46208530381679 31.0 28.0 33.0 16.0 34.0 4 32.07002664290456 35.0 32.0 37.0 22.0 37.0 5 32.31068775911522 35.0 33.0 37.0 23.0 37.0 6 31.46260599456406 35.0 32.0 37.0 17.0 37.0 7 31.513143415409225 35.0 32.0 37.0 17.0 37.0 8 31.303589545388665 35.0 32.0 37.0 17.0 37.0 9 33.5348406525275 37.0 34.0 39.0 19.0 39.0 10 33.715504237993244 38.0 34.0 39.0 22.0 39.0 11 33.86471523941933 38.0 34.0 39.0 23.0 39.0 12 33.72502697392789 38.0 34.0 39.0 22.0 39.0 13 33.8458612242853 38.0 35.0 39.0 23.0 39.0 14 34.90818665421303 39.0 35.0 41.0 23.0 41.0 15 34.451582023107214 39.0 34.0 41.0 17.0 41.0 16 34.55071366840051 39.0 34.0 41.0 18.0 41.0 17 34.80335067179842 39.0 35.0 41.0 22.0 41.0 18 34.85596906631739 39.0 35.0 41.0 22.0 41.0 19 34.224467812902034 39.0 34.0 41.0 16.0 41.0 20 34.4912135673042 39.0 34.0 41.0 18.0 41.0 21 34.478754564990616 39.0 34.0 41.0 18.0 41.0 22 34.509661407869054 39.0 35.0 41.0 18.0 41.0 23 34.51350664641162 39.0 35.0 41.0 17.0 41.0 24 33.21400747575925 39.0 33.0 41.0 2.0 41.0 25 33.7897876619554 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.16210544008473704 1101 2 -0.26731254011488303 1101 3 -0.9046951855811649 1101 4 -0.42359419270995957 1101 5 -0.22544726864425968 1101 6 -2.8230557104357388 1101 7 -0.870931425924649 1101 8 -0.186472510335431 1101 9 -0.3724079995728218 1101 10 -9.156856654921974 1101 11 -3.8346519587045584 1101 12 -1.5717553946977674 1101 13 -0.6140829254643165 1101 14 -0.574051494460079 1101 15 -2.3614816917625134 1101 16 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0.4320864791071841 2215 2 -0.09357516637751218 2215 3 0.3096987538127749 2215 4 0.5728704537546889 2215 5 0.6578860646890732 2215 6 1.298913986533961 2215 7 0.8088665538733295 2215 8 -0.6203108941738158 2215 9 -0.17139789856271648 2215 10 -0.45433140239672554 2215 11 -0.5283388273914298 2215 12 -0.7727654957078656 2215 13 -0.4304970668784591 2215 14 -0.1419807873893717 2215 15 -0.14001704529786707 2215 16 -0.1084297723034311 2215 17 -0.5096327628676178 2215 18 -1.3522834923683078 2215 19 -0.0016463803794692922 2215 20 -3.820405114004611 2215 21 -2.182645088699644 2215 22 -1.287453424744804 2215 23 -1.3140889548330605 2215 24 -1.4788009739087222 2215 25 -1.064337880157602 2216 1 -0.6139174366170792 2216 2 -0.9731364494103474 2216 3 -0.24252259175476354 2216 4 -0.24616171947227983 2216 5 -0.3256805561967404 2216 6 -1.2827405530762235 2216 7 -9.750664143651584 2216 8 -10.676831743944422 2216 9 -5.737169403389192 2216 10 -3.266806198574507 2216 11 -2.3070020264017828 2216 12 -2.089337191450248 2216 13 -1.7794309573745508 2216 14 -1.8425671719680423 2216 15 -2.8920026681610196 2216 16 -1.962017725119928 2216 17 -2.120140930367107 2216 18 -1.7625530149811297 2216 19 -1.3495019856121289 2216 20 -2.1418821797439733 2216 21 -4.651944641605752 2216 22 -3.355470139627478 2216 23 -3.3327022819324057 2216 24 -3.034104247726944 2216 25 -2.485048360077343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28338.0 3 2.0 4 201.0 5 1384.0 6 1763.0 7 1477.0 8 1257.0 9 1167.0 10 997.0 11 937.0 12 944.0 13 977.0 14 944.0 15 944.0 16 1028.0 17 1146.0 18 1325.0 19 1595.0 20 2135.0 21 2691.0 22 3369.0 23 3995.0 24 4765.0 25 5315.0 26 6208.0 27 6925.0 28 7642.0 29 9200.0 30 10996.0 31 13420.0 32 17538.0 33 23072.0 34 31386.0 35 44485.0 36 64236.0 37 104904.0 38 150165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54016753134263 17.806885247665555 11.767184874477026 25.885762346514785 2 14.98235178184294 20.176482181570602 40.494866787307615 24.34629924927884 3 18.852542728189764 24.46243735677389 29.617054366347084 27.06796554868926 4 12.506759297391179 16.198320471270748 37.397088578130315 33.89783165320775 5 12.933928967734085 37.97115611778097 35.88231925144792 13.21259566303702 6 32.00385866822254 36.47980642228434 18.567961599450193 12.948373310042918 7 27.960278859498406 31.197002421976972 22.347540230132697 18.495178488391925 8 25.294235920120062 36.398006592033546 21.141542018828225 17.166215469018162 9 26.752643372062224 15.110173313575157 19.48471246841197 38.652470845950646 10 15.09331290342213 28.839826870822577 34.248681662126025 21.818178563629267 11 34.99614333548686 22.320155054020148 24.03909995651186 18.644601653981134 12 23.48562807487253 24.61634002251402 31.312917999223284 20.585113903390166 13 29.205441894426386 22.04461179435051 25.398012799404412 23.35193351181869 14 21.19165949313399 21.845420363049357 26.84159945021807 30.121320693598584 15 23.417940123232537 30.853232303890078 22.764732515958848 22.964095056918545 16 22.356649026131496 27.799323849084836 27.416326771674683 22.427700353108985 17 21.995271681452355 28.507179277007523 27.372347032094225 22.1252020094459 18 22.014628218825553 27.354983956204826 28.991218415735627 21.639169409233997 19 24.163586737661642 26.621146360016105 26.649604009127927 22.56566289319433 20 23.931492385060043 27.199205397569663 27.32358573294916 21.545716484421142 21 23.183459739218513 26.6037560265005 26.635968946744885 23.576815287536103 22 23.658656186467372 27.521082094894485 26.65489075967128 22.16537095896687 23 22.780442408189565 27.34018183119765 27.316200157491586 22.563175603121195 24 23.803413526326246 26.629406722180764 27.449053915962896 22.118125835530094 25 23.025382127817977 26.844781210460667 27.545237025729314 22.58459963599205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 358.0 1 358.0 2 446.0 3 534.0 4 534.0 5 534.0 6 692.0 7 850.0 8 850.0 9 850.0 10 1087.5 11 1325.0 12 1325.0 13 1325.0 14 1957.0 15 2589.0 16 2589.0 17 2589.0 18 4360.0 19 6131.0 20 6131.0 21 6131.0 22 9262.0 23 12393.0 24 12393.0 25 12393.0 26 17943.0 27 23493.0 28 23493.0 29 23493.0 30 31177.0 31 38861.0 32 38861.0 33 38861.0 34 44174.5 35 49488.0 36 49488.0 37 49488.0 38 54273.0 39 59058.0 40 59058.0 41 59058.0 42 62766.5 43 66475.0 44 66475.0 45 66475.0 46 69553.5 47 72632.0 48 72632.0 49 72632.0 50 70893.5 51 69155.0 52 69155.0 53 69155.0 54 62946.5 55 56738.0 56 56738.0 57 56738.0 58 49567.5 59 42397.0 60 42397.0 61 42397.0 62 35218.0 63 28039.0 64 28039.0 65 28039.0 66 22008.5 67 15978.0 68 15978.0 69 15978.0 70 11744.0 71 7510.0 72 7510.0 73 7510.0 74 5306.0 75 3102.0 76 3102.0 77 3102.0 78 2151.0 79 1200.0 80 1200.0 81 1200.0 82 811.5 83 423.0 84 423.0 85 423.0 86 264.5 87 106.0 88 106.0 89 106.0 90 67.5 91 29.0 92 29.0 93 29.0 94 18.0 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22294868422700684 2 0.13294612550615256 3 0.13455650926060125 4 0.06853077532820516 5 0.025229345486362735 6 0.023440030203641972 7 0.3381805884342239 8 0.3286972174358038 9 0.020934988807832906 10 0.038470278578496366 11 0.017893152827207614 12 0.019682468109928374 13 0.017356358242391384 14 0.018966741996840068 15 0.016461700601031005 16 0.021829646449193288 17 0.020040331166472525 18 0.01610383754448685 19 0.026481866184267268 20 0.01842994741202384 21 0.016282769072758926 22 0.018787810468567992 23 0.0202192626947446 24 0.017177426714119308 25 0.016819563657575157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 558873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.15562245678586 #Duplication Level Percentage of deduplicated Percentage of total 1 90.37881677692495 74.2512794921607 2 6.978138745116638 11.46586664389744 3 1.4037196944694705 3.4597039576196584 4 0.48654331328347167 1.5988907501996232 5 0.2244667188046305 0.922060150211337 6 0.13597865965709416 0.6702846854980803 7 0.08370608547443552 0.48138478889012193 8 0.0572455165023232 0.37624328328868556 9 0.04433561763182703 0.32781782371939194 >10 0.1795766106147644 2.576252872713062 >50 0.012209913395080032 0.670114988086375 >100 0.013082012678846336 1.9008937689444239 >500 0.0017442683571707472 0.9296681738400592 >1k 4.360670892926868E-4 0.3695386209310293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1034 0.1850152002333267 No Hit GTCCTACAGTGGACATTTCTAAATT 1029 0.18412054259196634 No Hit GTCCTAAAGTGTGTATTTCTCATTT 886 0.15853333404905945 No Hit CTGTAGGACGTGGAATATGGCAAGA 825 0.14761851082446278 No Hit CTTTAGGACGTGAAATATGGCGAGG 617 0.11040075294387096 No Hit GTCCTACAGTGTGCATTTCTCATTT 599 0.10717998543497359 No Hit GTACATGGGAAGCAGTGGTATCAAC 598 0.10700105390670152 No Hit TATCAACGCAGAGTACTTTTTTTTT 587 0.10503280709570868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7893152827207613E-4 0.0 5 0.0 0.0 0.0 1.7893152827207613E-4 0.0 6 0.0 0.0 0.0 1.7893152827207613E-4 0.0 7 0.0 0.0 0.0 1.7893152827207613E-4 0.0 8 0.0 0.0 0.0 1.7893152827207613E-4 0.0 9 0.0 0.0 0.0 1.7893152827207613E-4 0.0 10 0.0 0.0 0.0 1.7893152827207613E-4 0.0 11 0.0 0.0 0.0 1.7893152827207613E-4 0.0 12 0.0 0.0 0.0 1.7893152827207613E-4 0.0 13 0.0 0.0 0.0 1.7893152827207613E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTCGA 35 0.0021956926 16.254639 17 GTGCTAC 55 1.0772066E-5 15.617851 1 TATGTCG 40 0.0053407447 14.222809 16 TGCCGGT 55 1.9870617E-4 13.79305 10 TAGGACC 215 0.0 13.726832 4 GTCCTAT 70 6.9030393E-6 13.634631 1 GTCCAAA 50 0.0014459027 13.361938 1 TCCTAAT 65 5.25232E-5 13.203207 2 GTTCTAA 75 1.4057414E-5 12.7256565 1 GCCGGTT 60 4.1510758E-4 12.642497 11 TTAGGAC 255 0.0 12.339142 3 GTCCTAA 290 0.0 12.177136 1 AGGCTTA 55 0.0030218728 12.113381 6 CTAGGCT 65 7.867612E-4 11.717197 4 TAGAACT 90 7.2624225E-6 11.635828 4 TAGGCTT 75 2.0321777E-4 11.4222145 5 GTATAGT 60 0.0056681116 11.13495 1 TGTAGGA 480 0.0 11.124926 2 AGTGCAA 60 0.0059477147 11.063176 10 TTACAAT 120 1.3024874E-7 11.062184 16 >>END_MODULE