FastQCFastQC Report
Thu 26 May 2016
SRR1926819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558873
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19290.34515891803683485No Hit
GGTATCAACGCAGAGTACTTTTTTT11200.20040331166472528No Hit
TATCAACGCAGAGTACTTTTTTTTT10040.1796472543851644No Hit
GTCCTACAGTGGACATTTCTAAATT9720.173921445480458No Hit
CTGTAGGACGTGGAATATGGCAAGA9660.17284785631082555No Hit
GTCCTAAAGTGTGTATTTCTCATTT8580.15352325125744132No Hit
CTTTAGGACGTGAAATATGGCGAGG8360.14958675763545565No Hit
GTACATGGGAAGCAGTGGTATCAAC7490.13401971467578502No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6060.10843250613287814No Hit
CCCATGTACTCTGCGTTGATACCAC6050.10825357460460605No Hit
GTCCTACAGTGTGCATTTCTCATTT6020.10771678001978983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC250.005874253319.0847971
CTAGAAC456.5911293E-414.8250473
TCCAACG400.005328363314.22778718
ATCCCGT551.9826797E-413.79664210
ATTAATC707.0074893E-613.6148393
TCCCGTT604.1388482E-412.64692111
CCCGTTT604.1419736E-412.6457912
CCGTTTC701.1053804E-412.19415613
CCCTAGA550.002963660812.1448711
GCACCGT550.003001853812.1241346
ACTACAC657.7974785E-411.7297083
GTCCTAA2550.011.6005621
GGTATCA4250.011.4508781
TCCTAAA3000.011.1307942
TTTACAC600.005729777711.1187853
ATAGGAC700.001449651810.8918713
TAGGACT1053.3409015E-610.8869784
TGAGACA700.001455099810.8869774
AATCCCG1053.5177964E-610.840219519
TAGGACC1855.456968E-1210.81341654
Produced by FastQC (version 0.11.5)