##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926819_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 558873 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.779447208936556 31.0 30.0 33.0 26.0 34.0 2 30.035856088950442 31.0 30.0 33.0 26.0 34.0 3 30.069378910772215 31.0 30.0 34.0 26.0 34.0 4 33.80739094570681 35.0 35.0 37.0 28.0 37.0 5 33.84432778108801 37.0 35.0 37.0 30.0 37.0 6 33.81114671848524 37.0 35.0 37.0 30.0 37.0 7 33.79314620674106 37.0 35.0 37.0 29.0 37.0 8 33.82121877421167 37.0 35.0 37.0 30.0 37.0 9 35.34791804220279 39.0 35.0 39.0 30.0 39.0 10 35.213619194342904 39.0 35.0 39.0 29.0 39.0 11 35.28686481544108 39.0 35.0 39.0 30.0 39.0 12 35.17725493985217 39.0 35.0 39.0 29.0 39.0 13 35.17937348914691 39.0 35.0 39.0 29.0 39.0 14 36.276703293950504 40.0 36.0 41.0 29.0 41.0 15 36.25265131792017 40.0 36.0 41.0 29.0 41.0 16 36.227872879885055 40.0 36.0 41.0 29.0 41.0 17 36.21476256680856 40.0 36.0 41.0 29.0 41.0 18 36.19114539439193 40.0 36.0 41.0 29.0 41.0 19 36.258702782206335 40.0 37.0 41.0 29.0 41.0 20 36.229833969434914 40.0 37.0 41.0 29.0 41.0 21 36.18057411970161 40.0 37.0 41.0 29.0 41.0 22 36.09113877392538 40.0 37.0 41.0 29.0 41.0 23 36.010059530519456 40.0 37.0 41.0 28.0 41.0 24 35.94444176047152 40.0 36.0 41.0 28.0 41.0 25 35.826855117352245 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0010558917536229728 1101 2 -0.1790298809793036 1101 3 0.026541907945187404 1101 4 -0.02215231631193859 1101 5 -0.0069200410890601916 1101 6 0.14580368588315196 1101 7 0.16975694325913082 1101 8 0.17126884221221417 1101 9 0.17464343625164247 1101 10 0.11853831957810002 1101 11 0.19665233090244527 1101 12 0.21267003032909315 1101 13 0.11363843497058213 1101 14 0.3242554873869352 1101 15 0.18073413510244052 1101 16 0.47636601113624977 1101 17 0.29268091422100895 1101 18 0.26862109050627936 1101 19 0.21268031224920492 1101 20 0.2377646524382655 1101 21 0.23862424839386875 1101 22 0.07973143160408824 1101 23 0.22355966569428176 1101 24 0.09011180396455387 1101 25 0.030715838266964113 1102 1 -0.6417453602025596 1102 2 -0.5311781239726159 1102 3 -0.4770325692208388 1102 4 -0.42372081823255314 1102 5 -0.20180907224569467 1102 6 -0.29063819774182775 1102 7 -0.17063642706381899 1102 8 -0.25210219207431805 1102 9 -0.39894455635043613 1102 10 -0.14275418291158104 1102 11 -0.2899745007948056 1102 12 -0.3215097961042588 1102 13 -0.00833837538651494 1102 14 -0.28148501624089306 1102 15 -0.29010097657774025 1102 16 -0.3518278998041424 1102 17 -0.2706993674691276 1102 18 -0.22360753376459996 1102 19 -0.16025680540622744 1102 20 -0.3977098105009844 1102 21 -0.33492817727123736 1102 22 -0.04498403290011055 1102 23 -0.14802590835608243 1102 24 -0.13956169155401454 1102 25 -0.20173187833567852 1103 1 -1.1603289732071502 1103 2 -1.0887893324577007 1103 3 -0.9562961970698289 1103 4 -1.0683340204296528 1103 5 -0.8350206836501783 1103 6 -0.7859511269883441 1103 7 -0.7544775598649593 1103 8 -0.7982459417630494 1103 9 -0.8131675468541957 1103 10 -0.6673237515725532 1103 11 -0.8463181518421905 1103 12 -0.9199896911010228 1103 13 -0.8515355761570049 1103 14 -0.7958064874429169 1103 15 -0.8681296596835608 1103 16 -0.8878295834545753 1103 17 -0.703897162658194 1103 18 -1.0150389097471972 1103 19 -0.897925431512121 1103 20 -0.7347997620960314 1103 21 -0.869083950280455 1103 22 -0.8032372134410934 1103 23 -0.6156051949574035 1103 24 -0.5759227574589687 1103 25 -0.5638195406159667 1104 1 -0.4091952684975375 1104 2 -0.44063239258181497 1104 3 -0.17544416904088678 1104 4 -0.25403192319154044 1104 5 -0.17839833756735146 1104 6 -0.14200685192738405 1104 7 -0.14634324784105956 1104 8 -0.30747126152789406 1104 9 -0.09692358531538048 1104 10 -0.19210186606208168 1104 11 -0.2233585891084715 1104 12 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0.22409502313554697 2210 2 0.22097757152531372 2210 3 0.2833738482334276 2210 4 0.36858393381704957 2210 5 0.23583526452095782 2210 6 0.2025201123498661 2210 7 0.3466636379013508 2210 8 0.24877345703237808 2210 9 0.3248372013716292 2210 10 0.11546129507656389 2210 11 0.030925826356416053 2210 12 0.14253336285984375 2210 13 0.2610418103818972 2210 14 0.30964571199395863 2210 15 0.4218111940001634 2210 16 0.23107972030512514 2210 17 0.21157473322833908 2210 18 0.16876756088544198 2210 19 0.21912816191498763 2210 20 0.4341524807784225 2210 21 0.298995268469767 2210 22 0.4827700470433811 2210 23 0.493143987917648 2210 24 0.23543994347543418 2210 25 0.31058461685879024 2211 1 0.3335771394731104 2211 2 0.18813293662404362 2211 3 0.3872581007098397 2211 4 0.48410519269887686 2211 5 0.1784190324658752 2211 6 0.1381834304655385 2211 7 0.05514718933219598 2211 8 -0.02489433137387209 2211 9 0.14818946926904175 2211 10 0.2177629446837841 2211 11 -0.16143292696254719 2211 12 0.015388848466407978 2211 13 -0.071222286784419 2211 14 0.11457053210726542 2211 15 0.15197740480386557 2211 16 0.057068409569922096 2211 17 0.12778303676806502 2211 18 0.04704964922117938 2211 19 0.09490008709012443 2211 20 -0.02380478644394657 2211 21 0.11773473653519062 2211 22 -0.05225472956709609 2211 23 0.22477779411504883 2211 24 0.3059207747294721 2211 25 0.42256939586942366 2212 1 -0.04321175747585926 2212 2 -0.16371589047447088 2212 3 0.12463433714448868 2212 4 0.12597150643021138 2212 5 -0.11150597678186358 2212 6 0.003802980905348363 2212 7 0.006864386282600776 2212 8 -0.049649446454168356 2212 9 0.0032870148952213185 2212 10 0.02006310946167389 2212 11 0.1299272832613667 2212 12 -0.12020341513977684 2212 13 -0.01841409689111373 2212 14 0.04482817170007536 2212 15 -0.10237503731970321 2212 16 -0.276738920403794 2212 17 -0.0778950746755882 2212 18 -0.2525626663645042 2212 19 -0.18625037962041802 2212 20 -0.1759168738386805 2212 21 -0.3427125657673784 2212 22 -0.24104310768190373 2212 23 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2214 9 -0.46499988492694655 2214 10 -0.3916517554459986 2214 11 -0.3835480127833719 2214 12 -0.5575634245516667 2214 13 -0.2927752873145124 2214 14 -0.4283535909482836 2214 15 -0.4612667869814686 2214 16 -0.4295237998365522 2214 17 -0.580951677253907 2214 18 -0.6571563938811664 2214 19 -0.37658893625241063 2214 20 -0.45663787910119424 2214 21 -0.47964768253366685 2214 22 -0.215885526357269 2214 23 -0.351144848325589 2214 24 -0.3436396105959929 2214 25 -0.4176841365852866 2215 1 0.21166195235968388 2215 2 0.022485270535849367 2215 3 0.16921867562195558 2215 4 0.27304970389008787 2215 5 -0.051617010786024764 2215 6 -0.14359025351078714 2215 7 -0.17847537997319307 2215 8 -0.07039782445445297 2215 9 -0.4339424223342121 2215 10 -0.3226232965835152 2215 11 -0.35183251758240175 2215 12 -0.3444006767416141 2215 13 -0.11287671654456943 2215 14 -0.4600879469564987 2215 15 -0.43037697600866665 2215 16 -0.5562097464395066 2215 17 -0.2840867625466643 2215 18 -0.42961123272604596 2215 19 -0.4067641321952422 2215 20 -0.46678080210718775 2215 21 -0.4341030243334103 2215 22 -0.3260766492039906 2215 23 -0.32517770804309265 2215 24 -0.41923163037888145 2215 25 -0.6139811314300019 2216 1 -0.2637108693969381 2216 2 -0.01879445049314654 2216 3 -2.150677489500822 2216 4 -1.5520791156456752 2216 5 -0.6586214817249214 2216 6 -0.3911497442718215 2216 7 -0.4359615239588521 2216 8 -0.44248319113962964 2216 9 -0.39428840871684656 2216 10 -0.2568439992709486 2216 11 -0.36277511618170166 2216 12 -0.3107244740200912 2216 13 -0.5053978780146267 2216 14 -0.49616910569918105 2216 15 -0.4897362388394839 2216 16 -0.4117058842885868 2216 17 -0.33640648825115704 2216 18 -0.36375498461328704 2216 19 -0.3017710967296878 2216 20 -0.24782655374117724 2216 21 -0.19660071093909437 2216 22 -0.43673384800005266 2216 23 -0.6982390836117034 2216 24 -0.6250740468288214 2216 25 -0.7044723502722334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13520.0 3 8.0 4 184.0 5 1506.0 6 2004.0 7 1524.0 8 1120.0 9 1007.0 10 859.0 11 792.0 12 748.0 13 712.0 14 698.0 15 697.0 16 659.0 17 750.0 18 790.0 19 1009.0 20 1237.0 21 1666.0 22 2094.0 23 2546.0 24 3112.0 25 3639.0 26 4354.0 27 4771.0 28 5518.0 29 6722.0 30 8673.0 31 11353.0 32 14597.0 33 19674.0 34 26360.0 35 37226.0 36 55927.0 37 98461.0 38 222356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96081979254376 18.70886257367563 9.984413909242372 28.34590372453824 2 15.099615581661133 20.68442944508335 38.6569720159213 25.55898295733422 3 18.71804462165369 24.702614770923446 29.183033964641268 27.3963066427816 4 12.530217061836947 16.854097442531664 36.91983688601883 33.69584860961256 5 13.178157248201213 38.5735681731559 34.77612020420543 13.472154374437459 6 32.50789227006468 37.172681791572245 17.15576593628002 13.163660002083056 7 28.497234068827034 31.818320110673042 21.404781633715906 18.27966418678402 8 26.09794984941942 37.229102582685115 19.265009296091705 17.407938271803765 9 26.963772848873102 15.199119597018798 19.047482709565436 38.789624844542665 10 15.415185968528677 29.190599400921162 33.204857047765266 22.189357582784893 11 35.06907849414072 22.527408621525723 23.68226576745035 18.721247116883212 12 23.909632097568135 24.62070853560124 30.897649010236712 20.57201035659392 13 29.47232809061141 21.893105731207616 25.274571904020615 23.35999427416036 14 21.571627185424955 21.923406570007856 26.453952865856824 30.051013378710365 15 23.407175449583967 30.706629685962245 22.83224478840476 23.05395007604903 16 22.48873174668215 27.809995508802572 27.072973906321852 22.628298838193427 17 22.0359939306317 28.593309380323223 27.151655477461745 22.21904121158333 18 22.209458371356487 27.277184318356685 28.9371410166944 21.576216293592427 19 23.855909761090196 26.433780901530223 27.121415995075797 22.588893342303784 20 23.95015647674142 27.04104181838678 27.429326013788565 21.579475691083243 21 23.207408732999284 26.238367931281314 26.883500357909806 23.670722977809593 22 23.800219370053664 27.367935112270587 26.470951361042673 22.360894156633083 23 22.73146135168455 27.324812613957018 27.449706820690928 22.494019213667507 24 23.74890851572453 26.525036144233383 27.538506133783763 22.18754920625832 25 23.147892994542364 26.757806209179563 27.472488145298378 22.621812650979688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 248.0 2 245.0 3 242.0 4 242.0 5 242.0 6 447.5 7 653.0 8 653.0 9 653.0 10 947.5 11 1242.0 12 1242.0 13 1242.0 14 1713.5 15 2185.0 16 2185.0 17 2185.0 18 3873.5 19 5562.0 20 5562.0 21 5562.0 22 8715.5 23 11869.0 24 11869.0 25 11869.0 26 17395.5 27 22922.0 28 22922.0 29 22922.0 30 30523.5 31 38125.0 32 38125.0 33 38125.0 34 43309.0 35 48493.0 36 48493.0 37 48493.0 38 53037.0 39 57581.0 40 57581.0 41 57581.0 42 61959.0 43 66337.0 44 66337.0 45 66337.0 46 69890.0 47 73443.0 48 73443.0 49 73443.0 50 71830.5 51 70218.0 52 70218.0 53 70218.0 54 63906.0 55 57594.0 56 57594.0 57 57594.0 58 50514.0 59 43434.0 60 43434.0 61 43434.0 62 36250.5 63 29067.0 64 29067.0 65 29067.0 66 22789.5 67 16512.0 68 16512.0 69 16512.0 70 12295.0 71 8078.0 72 8078.0 73 8078.0 74 5668.0 75 3258.0 76 3258.0 77 3258.0 78 2261.5 79 1265.0 80 1265.0 81 1265.0 82 833.0 83 401.0 84 401.0 85 401.0 86 259.0 87 117.0 88 117.0 89 117.0 90 69.5 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.122210233809828 2 0.11219006822659172 3 0.015388111431398547 4 0.0 5 0.004294356678529827 6 0.35768412501588015 7 0.019682468109928374 8 0.0064415350177947405 9 0.006799398074338893 10 0.002683972924081142 11 0.002862904452353218 12 3.5786305654415227E-4 13 5.367945848162284E-4 14 0.0 15 0.004115425150257751 16 3.5786305654415227E-4 17 1.7893152827207613E-4 18 5.367945848162284E-4 19 1.7893152827207613E-4 20 7.157261130883045E-4 21 0.013062001563861557 22 0.0 23 0.0 24 1.7893152827207613E-4 25 0.004115425150257751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 558873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.68804938265379 #Duplication Level Percentage of deduplicated Percentage of total 1 87.96595792387033 69.21869641105954 2 8.700748287197513 13.692898217780765 3 1.7907680943554223 4.227361447245609 4 0.6219651358126326 1.9576489328445363 5 0.2942572918483614 1.1577266156084918 6 0.1742627843344816 0.8227439147562261 7 0.09775123029171418 0.5384297545486698 8 0.07345086985547662 0.46237645395092974 9 0.04777524172513539 0.33834065221221077 >10 0.20053800844715683 2.7532532780195274 >50 0.01546651983005653 0.7923221146314546 >100 0.01455668260841014 2.2042502497757583 >500 0.0018195853260416564 1.1085682077843284 >1k 6.823444972656211E-4 0.7253837497820284 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1929 0.34515891803683485 No Hit GGTATCAACGCAGAGTACTTTTTTT 1120 0.20040331166472528 No Hit TATCAACGCAGAGTACTTTTTTTTT 1004 0.1796472543851644 No Hit GTCCTACAGTGGACATTTCTAAATT 972 0.173921445480458 No Hit CTGTAGGACGTGGAATATGGCAAGA 966 0.17284785631082555 No Hit GTCCTAAAGTGTGTATTTCTCATTT 858 0.15352325125744132 No Hit CTTTAGGACGTGAAATATGGCGAGG 836 0.14958675763545565 No Hit GTACATGGGAAGCAGTGGTATCAAC 749 0.13401971467578502 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 606 0.10843250613287814 No Hit CCCATGTACTCTGCGTTGATACCAC 605 0.10825357460460605 No Hit GTCCTACAGTGTGCATTTCTCATTT 602 0.10771678001978983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAGC 25 0.0058742533 19.084797 1 CTAGAAC 45 6.5911293E-4 14.825047 3 TCCAACG 40 0.0053283633 14.227787 18 ATCCCGT 55 1.9826797E-4 13.796642 10 ATTAATC 70 7.0074893E-6 13.614839 3 TCCCGTT 60 4.1388482E-4 12.646921 11 CCCGTTT 60 4.1419736E-4 12.64579 12 CCGTTTC 70 1.1053804E-4 12.194156 13 CCCTAGA 55 0.0029636608 12.144871 1 GCACCGT 55 0.0030018538 12.124134 6 ACTACAC 65 7.7974785E-4 11.729708 3 GTCCTAA 255 0.0 11.600562 1 GGTATCA 425 0.0 11.450878 1 TCCTAAA 300 0.0 11.130794 2 TTTACAC 60 0.0057297777 11.118785 3 ATAGGAC 70 0.0014496518 10.891871 3 TAGGACT 105 3.3409015E-6 10.886978 4 TGAGACA 70 0.0014550998 10.886977 4 AATCCCG 105 3.5177964E-6 10.8402195 19 TAGGACC 185 5.456968E-12 10.8134165 4 >>END_MODULE