FastQCFastQC Report
Thu 26 May 2016
SRR1926818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772186
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27200.3522467384801071No Hit
GGTATCAACGCAGAGTACTTTTTTT16890.21872968429886064No Hit
GTCCTACAGTGGACATTTCTAAATT15960.20668595390229816No Hit
TATCAACGCAGAGTACTTTTTTTTT15460.20021083003317852No Hit
CTGTAGGACGTGGAATATGGCAAGA14970.1938652086414413No Hit
CTTTAGGACGTGAAATATGGCGAGG13150.1702957577578459No Hit
GTCCTAAAGTGTGTATTTCTCATTT13150.1702957577578459No Hit
GTCCTACAGTGTGCATTTCTCATTT13030.1687417280292572No Hit
CTGTAGGACCTGGAATATGGCGAGA10620.13753163098010066No Hit
GTACATGGGAAGCAGTGGTATCAAC9110.1179767568953594No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8960.11603421973462352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4450.014.9931664
ATTAGAC653.2535354E-614.6636453
AAAGGCG400.005161748314.2970545
AAGGCGT400.005161748314.2970546
AGGACCT6050.014.0213555
TCCAACG1101.9099389E-1013.79557318
TTAGTAC603.9757192E-412.7084933
CCAACGT604.144734E-412.64594319
GGACCTG6250.012.5051576
TTAGCCA550.002991240212.1308354
GTAGGAC8300.012.0577573
TCTAGAC907.1783816E-611.6494523
TGCCGGT907.583818E-611.59211410
TGTAGGA9400.011.4661322
AGGACTC752.0087525E-411.4376445
GTCCTAA3850.011.3985161
GACCTGG6000.011.3820867
GTCCTAC9450.011.3067421
TGGCGAG7500.011.25488918
CTAGAGT1201.2146484E-711.1199314
Produced by FastQC (version 0.11.5)