##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 772186 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.999853662200557 31.0 30.0 33.0 26.0 34.0 2 30.321346152351893 31.0 30.0 34.0 26.0 34.0 3 30.364216652464563 31.0 30.0 34.0 26.0 34.0 4 33.93245021277257 37.0 35.0 37.0 30.0 37.0 5 33.906222076028314 37.0 35.0 37.0 30.0 37.0 6 33.88184841476017 37.0 35.0 37.0 30.0 37.0 7 33.87137554941426 37.0 35.0 37.0 30.0 37.0 8 33.90157164206551 37.0 35.0 37.0 30.0 37.0 9 35.43867798690989 39.0 35.0 39.0 30.0 39.0 10 35.33497499307162 39.0 35.0 39.0 30.0 39.0 11 35.36347848834348 39.0 35.0 39.0 30.0 39.0 12 35.27161202093796 39.0 35.0 39.0 30.0 39.0 13 35.290911775142256 39.0 35.0 39.0 30.0 39.0 14 36.39521048037649 40.0 37.0 41.0 30.0 41.0 15 36.399661480524124 40.0 37.0 41.0 30.0 41.0 16 36.38661799100217 40.0 37.0 41.0 30.0 41.0 17 36.37001706842652 40.0 37.0 41.0 30.0 41.0 18 36.3466366911599 40.0 37.0 41.0 30.0 41.0 19 36.417284177646316 40.0 37.0 41.0 30.0 41.0 20 36.37445900340074 40.0 37.0 41.0 29.0 41.0 21 36.32885341096575 40.0 37.0 41.0 29.0 41.0 22 36.23644821325432 40.0 37.0 41.0 29.0 41.0 23 36.17970152269013 40.0 37.0 41.0 29.0 41.0 24 36.116844905243035 40.0 37.0 41.0 29.0 41.0 25 36.014070444167594 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4476186189274749 1101 2 -0.3495740601455104 1101 3 -0.20024975067050477 1101 4 -0.3954849161554179 1101 5 -0.3452615477164045 1101 6 -0.44115493698317465 1101 7 -0.3851650723684159 1101 8 -0.5234297475136103 1101 9 -0.42824187413224024 1101 10 -0.3938355505226099 1101 11 -0.24574986449511016 1101 12 -0.24986886186013635 1101 13 -0.3081878058109666 1101 14 -0.40599158621733267 1101 15 -0.46786617477632575 1101 16 -0.5561416935686196 1101 17 -0.5402484952760389 1101 18 -0.6234366485595118 1101 19 -0.5201472456285217 1101 20 -0.4668663830741622 1101 21 -0.5481101949367471 1101 22 -0.5384732060204911 1101 23 -0.7201475490850839 1101 24 -0.5122062708422348 1101 25 -0.54341408350205 1102 1 -0.7677736152674868 1102 2 -0.7469718600286157 1102 3 -0.3804377543154267 1102 4 -0.6770216829780864 1102 5 -0.8359069666244707 1102 6 -0.9660472191266365 1102 7 -0.7288264723000495 1102 8 -0.6947346463464541 1102 9 -0.7550395707584698 1102 10 -0.6043074788486962 1102 11 -0.6642518784799947 1102 12 -0.6607170195338625 1102 13 -0.5519168540633288 1102 14 -0.7300175257398962 1102 15 -0.7468817925768008 1102 16 -0.7400237872246649 1102 17 -0.7598660324489046 1102 18 -0.4657954416119594 1102 19 -0.5994575658437924 1102 20 -0.6388311107797549 1102 21 -0.7134647379616297 1102 22 -0.6453892753120414 1102 23 -0.4967645461816801 1102 24 -0.4735285615587159 1102 25 -0.6384246827963835 1103 1 -0.6678232690810972 1103 2 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14 0.6387724151135714 2208 15 0.5447165653964348 2208 16 0.6385471628238122 2208 17 0.7292067583728681 2208 18 0.8718049230775335 2208 19 0.8672040699148766 2208 20 0.7682227355932554 2208 21 0.9760101661495213 2208 22 0.9272879660152284 2208 23 0.995941278916753 2208 24 0.8579785008350669 2208 25 0.6800356437454838 2209 1 0.275277100010463 2209 2 0.22660733232875785 2209 3 0.4756955713005411 2209 4 0.3445470848429366 2209 5 0.20206323418950944 2209 6 -0.11088948023355272 2209 7 0.12867008534866642 2209 8 0.06540125037081879 2209 9 0.1432975907944254 2209 10 -0.0061235476226073615 2209 11 0.22567208515902593 2209 12 0.1423439314080639 2209 13 0.2716230406555553 2209 14 0.3031120589845955 2209 15 0.07723309665869493 2209 16 0.16621250429679435 2209 17 0.08614282018190522 2209 18 0.17785037395112369 2209 19 0.021204462279918346 2209 20 0.1526834933193868 2209 21 0.031033592650317132 2209 22 0.026041341508566518 2209 23 0.20605924808147336 2209 24 0.17161376291183217 2209 25 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2212 22 -0.18854495531456905 2212 23 -0.1238162314650566 2212 24 -0.06371426846882855 2212 25 -0.07035899435197734 2213 1 0.41030549333269306 2213 2 0.35365677513649274 2213 3 0.43495889267879306 2213 4 0.38249780214792395 2213 5 0.36680187686061316 2213 6 0.5029213092288956 2213 7 0.3957687895030588 2213 8 0.28448748708146354 2213 9 0.5006118066690703 2213 10 0.22395175872639328 2213 11 0.33934144171937675 2213 12 0.2622149872980799 2213 13 0.33585759511522895 2213 14 0.21848581544247736 2213 15 0.33906942943696805 2213 16 0.25088144456407946 2213 17 0.18486165157253254 2213 18 0.15908116042480458 2213 19 0.25285020755125487 2213 20 0.36842604274084323 2213 21 0.3747849269229775 2213 22 0.3418450006179157 2213 23 0.40413602746749433 2213 24 0.25175017894872553 2213 25 0.36795782311175884 2214 1 0.4173209429538538 2214 2 0.24489717389446142 2214 3 0.30995622813428625 2214 4 0.46361719505809873 2214 5 0.5223165365923634 2214 6 0.4736231620939364 2214 7 0.2762917981409245 2214 8 0.27303349843597857 2214 9 0.2553754201230021 2214 10 0.049927396499924726 2214 11 0.14610038452286034 2214 12 0.2654242831240978 2214 13 0.3020664338238319 2214 14 0.316966196105227 2214 15 0.29079143573240174 2214 16 0.2329532048924463 2214 17 0.3810810774046445 2214 18 0.23377722471493456 2214 19 0.28854207725620995 2214 20 0.25356759518736993 2214 21 0.37383516483719603 2214 22 0.41827005823128616 2214 23 0.31765083404524574 2214 24 0.25401681814097543 2214 25 0.42668793672793726 2215 1 0.4394557552804308 2215 2 0.1518039654875274 2215 3 0.21490520130916835 2215 4 0.43464281810403804 2215 5 0.42344169784247043 2215 6 0.31073189058047035 2215 7 0.3827664004834048 2215 8 0.16166935867957477 2215 9 -0.04283738883999888 2215 10 0.1808491293973944 2215 11 0.1316744126535596 2215 12 0.053196877895757666 2215 13 0.0698495801126029 2215 14 0.06734601733047185 2215 15 0.009277404329395722 2215 16 0.0043180792481365415 2215 17 0.012957789382731733 2215 18 0.055345282556835684 2215 19 -0.041183928337872544 2215 20 0.05526919427646959 2215 21 -0.03301263620660677 2215 22 0.07420267098950006 2215 23 -0.013754176144530561 2215 24 0.052974243659839715 2215 25 0.2711743794753829 2216 1 -0.30034193219539773 2216 2 -0.14367912179003284 2216 3 -1.8647367397530275 2216 4 -1.4658540723022284 2216 5 -0.6204960391556895 2216 6 -0.38017860468734455 2216 7 -0.25532712293745874 2216 8 -0.2376767153194237 2216 9 -0.041284259019462866 2216 10 -0.41318274004856903 2216 11 -0.2850294846742756 2216 12 -0.5115644680093183 2216 13 -0.4106576681970111 2216 14 -0.634696445627803 2216 15 -0.6788307890833352 2216 16 -0.5486533720586024 2216 17 -0.5055336242265795 2216 18 -0.48563178335588475 2216 19 -0.5643069473332929 2216 20 -0.2765576864831729 2216 21 -0.5096890161860657 2216 22 -0.3521650834792922 2216 23 -0.5348828542004469 2216 24 -0.4815663633221874 2216 25 -0.4803798617081725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18073.0 3 20.0 4 272.0 5 1964.0 6 2723.0 7 2007.0 8 1509.0 9 1299.0 10 1173.0 11 1137.0 12 980.0 13 900.0 14 924.0 15 913.0 16 880.0 17 1057.0 18 1129.0 19 1294.0 20 1768.0 21 2268.0 22 2893.0 23 3383.0 24 4092.0 25 4888.0 26 5819.0 27 6357.0 28 7336.0 29 9058.0 30 11486.0 31 14844.0 32 19275.0 33 25964.0 34 35114.0 35 49266.0 36 73804.0 37 130208.0 38 326109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.716562466775756 19.92054704294453 10.62398058277603 27.73890990750369 2 13.51099044292399 21.74257482227158 41.33231162991516 23.414123104889264 3 18.14744630114878 26.20444356941442 29.76644901733984 25.88166111209696 4 11.19678419448164 18.265806424876907 39.58644678872707 30.950962591914383 5 11.759990157161635 39.799128390760686 36.13590887603851 12.304972576039164 6 29.774014162940087 39.71809277258591 18.548149187820755 11.959743876653242 7 26.755006346985827 32.71249449496127 22.74435895443123 17.78814020362167 8 23.797854938291593 39.333297932000946 20.507473782507166 16.361373347200296 9 26.04967788896171 15.75352223952006 20.41862778366365 37.778172087854585 10 13.486954145573268 31.10543008517654 35.3431473488042 20.064468420445994 11 33.42204678112644 23.407658366455177 25.137894370128045 18.032400482290335 12 22.567490216087833 26.181904359830405 32.85905209366655 18.39155333041521 13 28.631074021409457 23.28039244634037 25.976518489164473 22.112015043085698 14 19.615222239201437 23.835707977093602 28.84602932454098 27.703040459163986 15 21.94337961044343 32.86792892664733 23.825113966017405 21.363577496891835 16 20.266645946243454 29.837927439667954 29.506400668233653 20.389025945854943 17 20.099043494702055 30.17977533910224 28.934738521547914 20.786442644647792 18 19.690953687084132 29.12681207280149 31.405937978352256 19.776296261762123 19 22.012471104722316 28.010127106846156 28.882586426827768 21.09481536160376 20 22.078214721399878 28.817170045403806 29.4756935541103 19.62892167908602 21 21.631871184833752 27.837162584851626 28.413136929578815 22.117829300735803 22 21.43304851421808 29.656067320567843 28.516186514648023 20.394697650566055 23 20.664062803521432 29.39175276423038 29.01114498320353 20.93303944904466 24 21.660843371933705 28.223251910809054 29.89448138142883 20.221423335828415 25 21.150929687712477 28.715610231809997 29.413669265069466 20.719790815408068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 441.0 1 441.0 2 425.0 3 409.0 4 409.0 5 409.0 6 724.0 7 1039.0 8 1039.0 9 1039.0 10 1589.5 11 2140.0 12 2140.0 13 2140.0 14 3369.5 15 4599.0 16 4599.0 17 4599.0 18 7878.0 19 11157.0 20 11157.0 21 11157.0 22 17435.0 23 23713.0 24 23713.0 25 23713.0 26 33913.5 27 44114.0 28 44114.0 29 44114.0 30 56941.0 31 69768.0 32 69768.0 33 69768.0 34 78169.5 35 86571.0 36 86571.0 37 86571.0 38 91315.0 39 96059.0 40 96059.0 41 96059.0 42 98805.0 43 101551.0 44 101551.0 45 101551.0 46 102855.0 47 104159.0 48 104159.0 49 104159.0 50 96796.0 51 89433.0 52 89433.0 53 89433.0 54 74807.5 55 60182.0 56 60182.0 57 60182.0 58 49386.0 59 38590.0 60 38590.0 61 38590.0 62 29967.0 63 21344.0 64 21344.0 65 21344.0 66 15878.5 67 10413.0 68 10413.0 69 10413.0 70 7347.0 71 4281.0 72 4281.0 73 4281.0 74 2886.0 75 1491.0 76 1491.0 77 1491.0 78 1007.5 79 524.0 80 524.0 81 524.0 82 340.0 83 156.0 84 156.0 85 156.0 86 98.5 87 41.0 88 41.0 89 41.0 90 26.0 91 11.0 92 11.0 93 11.0 94 5.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1181062593727418 2 0.11978979157871289 3 0.019295869129976457 4 0.0 5 0.006604626346502009 6 0.3513402211384304 7 0.021497411245477126 8 0.011266715532268132 9 0.011914227919180094 10 0.0032375619345598084 11 0.0047915916631485166 12 0.0 13 5.180099095295693E-4 14 0.0 15 0.0033670644119422007 16 1.2950247738239233E-4 17 0.0 18 5.180099095295693E-4 19 1.2950247738239233E-4 20 5.180099095295693E-4 21 0.01333875517038641 22 0.0 23 0.0 24 0.0 25 0.005827611482207655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 772186.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.48080577409853 #Duplication Level Percentage of deduplicated Percentage of total 1 93.0849697028281 77.70808276249637 2 5.450083084239912 9.099546548162639 3 0.7253437570313364 1.8165684390056367 4 0.224975880452613 0.7512467111968557 5 0.11915279833276285 0.49734858075288524 6 0.07785401431026062 0.3899589508421252 7 0.05144848390203976 0.3006472624398611 8 0.0407071002300954 0.27186092223482844 9 0.02542894099435271 0.19105456357715128 >10 0.16245122009111004 2.576850446599075 >50 0.02065683673143244 1.2452169725826892 >100 0.01444331029992726 2.662220235496581 >500 0.0010871308683067925 0.6687314642255703 >1k 0.0013977396878230189 1.820666140387686 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2720 0.3522467384801071 No Hit GGTATCAACGCAGAGTACTTTTTTT 1689 0.21872968429886064 No Hit GTCCTACAGTGGACATTTCTAAATT 1596 0.20668595390229816 No Hit TATCAACGCAGAGTACTTTTTTTTT 1546 0.20021083003317852 No Hit CTGTAGGACGTGGAATATGGCAAGA 1497 0.1938652086414413 No Hit CTTTAGGACGTGAAATATGGCGAGG 1315 0.1702957577578459 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1315 0.1702957577578459 No Hit GTCCTACAGTGTGCATTTCTCATTT 1303 0.1687417280292572 No Hit CTGTAGGACCTGGAATATGGCGAGA 1062 0.13753163098010066 No Hit GTACATGGGAAGCAGTGGTATCAAC 911 0.1179767568953594 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 896 0.11603421973462352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 445 0.0 14.993166 4 ATTAGAC 65 3.2535354E-6 14.663645 3 AAAGGCG 40 0.0051617483 14.297054 5 AAGGCGT 40 0.0051617483 14.297054 6 AGGACCT 605 0.0 14.021355 5 TCCAACG 110 1.9099389E-10 13.795573 18 TTAGTAC 60 3.9757192E-4 12.708493 3 CCAACGT 60 4.144734E-4 12.645943 19 GGACCTG 625 0.0 12.505157 6 TTAGCCA 55 0.0029912402 12.130835 4 GTAGGAC 830 0.0 12.057757 3 TCTAGAC 90 7.1783816E-6 11.649452 3 TGCCGGT 90 7.583818E-6 11.592114 10 TGTAGGA 940 0.0 11.466132 2 AGGACTC 75 2.0087525E-4 11.437644 5 GTCCTAA 385 0.0 11.398516 1 GACCTGG 600 0.0 11.382086 7 GTCCTAC 945 0.0 11.306742 1 TGGCGAG 750 0.0 11.254889 18 CTAGAGT 120 1.2146484E-7 11.119931 4 >>END_MODULE