FastQCFastQC Report
Thu 26 May 2016
SRR1926817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042951
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT29270.28064597473898584No Hit
GTCCTAAAGTGTGTATTTCTCATTT23280.22321278756144824No Hit
CTGTAGGACGTGGAATATGGCAAGA22570.21640518106795045No Hit
GTCCTACAGTGTGCATTTCTCATTT19970.1914759178523248No Hit
CTTTAGGACGTGAAATATGGCGAGG18590.17824423199172348No Hit
CTGTAGGACCTGGAATATGGCGAGA13470.12915275981326063No Hit
GTATCAACGCAGAGTACTTTTTTTT12840.12311220757255134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC350.002199635616.25217413
TAGTACG400.00518726314.2871074
CTAGCAC706.980692E-613.622523
CGCCACC500.001521885613.27324614
TAGGACC5350.013.1744044
AACACGG550.003021150412.1153915
AGGACCT9000.011.9519285
TCGAACT802.9238667E-511.85111219
CTGTGCG658.1495737E-411.6699089
CCAACGT658.1561337E-411.66878819
GTCCTAC15250.011.642861
ATGTCCA3850.011.5751849
TGGCGAG12850.011.43609318
TCCTACA16500.011.3862232
GGCGAGG5600.011.34320719
CCCTTAC855.063843E-511.2304621
GGACCTG9200.011.1747556
CTAGAGC600.00571141211.1250583
AATCCCG1451.2551027E-911.11552619
CTACTAG700.001431925210.9095931
Produced by FastQC (version 0.11.5)