##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926817_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1042951
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.62775336521083	31.0	30.0	33.0	22.0	34.0
2	28.74033008262133	31.0	30.0	33.0	22.0	34.0
3	28.539284204147656	31.0	28.0	33.0	16.0	34.0
4	32.09809377430004	35.0	32.0	37.0	22.0	37.0
5	32.32555891887538	35.0	33.0	37.0	23.0	37.0
6	31.57319854911688	35.0	32.0	37.0	17.0	37.0
7	31.599612062311653	35.0	32.0	37.0	17.0	37.0
8	31.416360883684852	35.0	32.0	37.0	17.0	37.0
9	33.57276612228187	37.0	34.0	39.0	19.0	39.0
10	33.72890001543697	38.0	34.0	39.0	22.0	39.0
11	33.88474051034037	38.0	34.0	39.0	23.0	39.0
12	33.733997090946744	38.0	34.0	39.0	22.0	39.0
13	33.84901112324548	38.0	35.0	39.0	23.0	39.0
14	34.913264381548125	39.0	35.0	41.0	24.0	41.0
15	34.50670645121391	39.0	34.0	41.0	17.0	41.0
16	34.59776442038025	39.0	34.0	41.0	18.0	41.0
17	34.835776560931436	39.0	35.0	41.0	23.0	41.0
18	34.884376159570294	39.0	35.0	41.0	22.0	41.0
19	34.287809302642216	39.0	34.0	41.0	17.0	41.0
20	34.54959533094076	39.0	34.0	41.0	18.0	41.0
21	34.53158873235655	39.0	34.0	41.0	18.0	41.0
22	34.567787940181276	39.0	35.0	41.0	18.0	41.0
23	34.573713434284066	39.0	35.0	41.0	18.0	41.0
24	33.33439346623187	39.0	33.0	41.0	2.0	41.0
25	33.896042095937396	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.10813691844310114
1101	2	-0.15397671856765172
1101	3	-0.9731433766082702
1101	4	-0.30952308000055595
1101	5	-0.26911600806676006
1101	6	-2.098886016304885
1101	7	-0.6925571878719587
1101	8	-0.24887616777397525
1101	9	-0.2044799916793565
1101	10	-9.603570703835793
1101	11	-4.196306621031972
1101	12	-1.5459033221849978
1101	13	-0.851260831719344
1101	14	-0.587717158942155
1101	15	-1.971886663856452
1101	16	-0.356443795759688
1101	17	-0.16383365989253207
1101	18	0.021065035392595632
1101	19	-1.0380417112334115
1101	20	-0.15428507190290475
1101	21	-0.30747097911469723
1101	22	0.1328533367185898
1101	23	-0.04863928505901782
1101	24	-0.1365146654897842
1101	25	-0.058899658059303306
1102	1	-0.20191008375073594
1102	2	-0.2203275903299371
1102	3	-0.3644222237803696
1102	4	-0.22564735602104946
1102	5	-0.12913441812223425
1102	6	-1.022347161041047
1102	7	-0.15569363136422254
1102	8	0.32861034373774345
1102	9	0.24876308350589937
1102	10	0.22931323950033544
1102	11	-0.017851273562541792
1102	12	0.08439398120348329
1102	13	-0.047417528987971025
1102	14	-0.22178336454282999
1102	15	0.2928515630759918
1102	16	0.1553638924749876
1102	17	-0.01714108881152754
1102	18	-0.20139658996006204
1102	19	0.1524724941065898
1102	20	-0.09296573890084403
1102	21	-0.0540245244080495
1102	22	-0.10012618684655195
1102	23	-0.769158079336151
1102	24	-3.9962751175408187
1102	25	-2.1204389342019887
1103	1	0.14588892696062672
1103	2	-0.15292374759781424
1103	3	0.20389042865740237
1103	4	0.13113846873744706
1103	5	-0.023271774307204396
1103	6	0.3560885913137817
1103	7	0.25504866359927547
1103	8	0.5026090790688151
1103	9	0.1436888047367333
1103	10	0.22589425358962245
1103	11	-0.020087711904459127
1103	12	0.019208783852171507
1103	13	0.07395510215299339
1103	14	-0.15741390602369876
1103	15	0.2513200544792511
1103	16	0.2894872332813634
1103	17	-0.03500932782847599
1103	18	-0.1823950761896782
1103	19	-1.7749431741012174
1103	20	-0.3962275992781059
1103	21	-0.24359210070563364
1103	22	-0.23971881015861385
1103	23	-0.11022040123545906
1103	24	1.3323471871677413
1103	25	0.5870762650928611
1104	1	-0.5739981379877115
1104	2	-0.5184492681508637
1104	3	-1.6426257083869586
1104	4	-0.7681952358233559
1104	5	-0.6235652843697395
1104	6	-1.8103962451124467
1104	7	-0.5456542649581309
1104	8	-0.09924412049799969
1104	9	-0.2502538668814154
1104	10	-0.06976572341563525
1104	11	-0.31291719074827284
1104	12	-0.2282833164362188
1104	13	-0.43909505673147464
1104	14	-0.5253800023621267
1104	15	-0.20797601099921792
1104	16	-0.6248932018737889
1104	17	-0.6153847482969965
1104	18	-0.5568540922766516
1104	19	-1.5602842293219723
1104	20	-0.9936901660623292
1104	21	-0.41221928461590096
1104	22	-0.4556392161408098
1104	23	-0.2580864442043662
1104	24	0.9624261375465579
1104	25	0.26548288914403884
1105	1	-0.14362648816382872
1105	2	-0.2070801328678833
1105	3	0.2267700213049828
1105	4	-0.005079580515676696
1105	5	0.10326259343918309
1105	6	-0.4101163709412923
1105	7	-0.2270316463064148
1105	8	0.11166681520984056
1105	9	-0.7238872427458958
1105	10	-0.29818512576254363
1105	11	-0.3031737689365812
1105	12	-0.41010269367165364
1105	13	-0.41183086705161287
1105	14	-0.7452305381866964
1105	15	-0.29611813515849406
1105	16	-0.7217386577149227
1105	17	-0.667291693316443
1105	18	-0.6679401296180885
1105	19	-1.3188883028103646
1105	20	-0.5378324904347096
1105	21	-0.5192454921724234
1105	22	-0.30695086650764125
1105	23	-0.415381836588125
1105	24	-5.411280960856484
1105	25	-2.794633217491498
1106	1	0.010720821727932872
1106	2	-0.655156542231321
1106	3	-0.40244471245443947
1106	4	-0.6117819306090055
1106	5	-0.027671177857094165
1106	6	0.16698431766309696
1106	7	-2.67723685898887
1106	8	-0.2992153107572726
1106	9	-0.32517730415305124
1106	10	0.19574341504254278
1106	11	-0.33051429831769497
1106	12	0.06906479747345173
1106	13	-0.09205814277698465
1106	14	-0.4722411937459938
1106	15	0.05771255654593688
1106	16	0.2334525883028462
1106	17	-0.18762580495742043
1106	18	-0.2914717335254551
1106	19	0.28458374077486326
1106	20	0.11826642389208786
1106	21	-0.03802834763805407
1106	22	-0.34302303475400464
1106	23	-0.38870913473931523
1106	24	-3.4779606542824233
1106	25	-1.647761041330135
1107	1	0.16216682817306705
1107	2	-0.040589465615735065
1107	3	0.15356218415618983
1107	4	0.037873015154822554
1107	5	0.23430118933251265
1107	6	-0.09594103128563702
1107	7	-2.462198131648627
1107	8	-1.7477439240040873
1107	9	-0.42302286579201365
1107	10	0.02506613094701038
1107	11	-0.2267938461414758
1107	12	0.016627616786401234
1107	13	-0.20898164270519715
1107	14	-0.1727096312585772
1107	15	-1.5177684389315687
1107	16	-0.7758771153210446
1107	17	-0.5543590542591517
1107	18	-0.698407553478205
1107	19	0.2700710040495977
1107	20	0.16084026370899096
1107	21	-0.23103013442801057
1107	22	-0.16674377977993515
1107	23	-0.32219117064675373
1107	24	-4.080391957638522
1107	25	-1.9692974122470694
1108	1	0.39930866150548283
1108	2	-0.12182708404854381
1108	3	0.5470569352753927
1108	4	0.4691758958902241
1108	5	0.5724239610433273
1108	6	-2.7376502042342743
1108	7	-1.0663545662916327
1108	8	0.4298049009208498
1108	9	0.09817814917234813
1108	10	0.6097641051831317
1108	11	0.40362506633159967
1108	12	0.5006715535216486
1108	13	0.4588913877366636
1108	14	0.28820213929473226
1108	15	-0.4418386377517436
1108	16	0.43943779788126847
1108	17	0.36689044576323937
1108	18	0.37317273722783284
1108	19	0.42360911668423995
1108	20	-0.7264013293984348
1108	21	0.03690678313245854
1108	22	0.241080781320278
1108	23	0.2936024637444703
1108	24	-0.5233465757813605
1108	25	0.16109438945611743
1109	1	0.5970411334146632
1109	2	0.578219015250248
1109	3	-0.46333988778992463
1109	4	0.5078258643664384
1109	5	0.8512098471253324
1109	6	0.9993205294903049
1109	7	1.1637114415768828
1109	8	1.4381539961467844
1109	9	0.978475143151563
1109	10	0.9544832873875961
1109	11	0.5313916833586632
1109	12	0.2536030395687021
1109	13	0.47724033984385983
1109	14	0.4730802887543817
1109	15	-0.7714200003777165
1109	16	0.4725324995502618
1109	17	0.4354108013907094
1109	18	0.46742019253900224
1109	19	-0.9947962543360234
1109	20	0.35236482136284764
1109	21	0.466775965073289
1109	22	0.7677878677316059
1109	23	0.5360535152529096
1109	24	-0.24838760943008253
1109	25	0.09406811812859672
1110	1	0.3908232666154703
1110	2	0.5147882987205463
1110	3	0.8761294447861161
1110	4	0.7169264749342332
1110	5	0.7995142199029956
1110	6	0.8087055688044771
1110	7	0.852602524447267
1110	8	1.1406804274608682
1110	9	0.7696381801346988
1110	10	0.7227211899215717
1110	11	0.46820797463262664
1110	12	0.6260360812257559
1110	13	0.4763122117192822
1110	14	0.2795104787285254
1110	15	0.8417121621090473
1110	16	0.574021276225622
1110	17	0.2550884488114704
1110	18	0.24679563256787418
1110	19	-1.8364979014491425
1110	20	0.06258288340173834
1110	21	0.15157699806879776
1110	22	0.24371348140548577
1110	23	0.48621710245701166
1110	24	-0.6735357401578526
1110	25	-0.27251956520444054
1111	1	0.9411490811156256
1111	2	0.6166015397987294
1111	3	1.233168857294853
1111	4	1.1121355651082467
1111	5	1.1338837872548382
1111	6	1.3630208602110017
1111	7	1.3263442437197703
1111	8	1.676918537000379
1111	9	1.1314850350465306
1111	10	1.0453511836262024
1111	11	1.0406914131776475
1111	12	1.1350329797431655
1111	13	1.106357174553473
1111	14	1.1077261732700308
1111	15	0.30486725645302926
1111	16	0.5705303034458993
1111	17	0.705245977559386
1111	18	0.8506469288951877
1111	19	1.5202906346164227
1111	20	1.0302414806306217
1111	21	1.129738835394015
1111	22	1.3458465448270474
1111	23	1.0551139102979334
1111	24	1.6798213837224054
1111	25	1.3238242084393121
1112	1	0.03395098474441838
1112	2	0.34771397287427774
1112	3	-1.3028216245611866
1112	4	-0.1704343337614702
1112	5	-0.05053025200750483
1112	6	0.9709923057791201
1112	7	1.03128178090002
1112	8	1.312254646459035
1112	9	0.08389431523689694
1112	10	0.5028169777963356
1112	11	0.3839394798046527
1112	12	0.5240871141869476
1112	13	0.06174087473193879
1112	14	0.42004027202077765
1112	15	0.7064139548468447
1112	16	0.6115772992817128
1112	17	-0.18410559888320677
1112	18	0.35867786286306824
1112	19	1.0884048523439702
1112	20	0.60192500234389
1112	21	0.704991661093004
1112	22	0.5778513038990312
1112	23	0.7124607276685424
1112	24	1.8529326270299578
1112	25	0.9294161180169453
1113	1	-0.48103319730048
1113	2	-0.007401284601009195
1113	3	-4.514604138852089
1113	4	-1.1123227942245535
1113	5	-0.10276085911832666
1113	6	0.729735489472155
1113	7	1.0128861106299816
1113	8	1.3107748855074668
1113	9	0.8420734505035412
1113	10	0.6943335924525158
1113	11	0.5087081697121008
1113	12	0.41347437674006216
1113	13	0.0628759009265849
1113	14	-0.17141855192439692
1113	15	0.521331234096202
1113	16	0.8324015652825167
1113	17	0.5684489896056988
1113	18	0.34271889565375346
1113	19	1.2200762061329513
1113	20	0.8082589751399851
1113	21	0.8524120230333168
1113	22	0.7706775641644157
1113	23	0.6587832739799353
1113	24	1.9438119847631228
1113	25	1.1654239578743102
1114	1	0.7177048561778818
1114	2	0.5549265578242242
1114	3	0.8689014424493422
1114	4	0.7614625280123732
1114	5	0.8702669948216268
1114	6	1.360116326308578
1114	7	1.606807673694771
1114	8	1.5180074644875994
1114	9	0.8152344746794427
1114	10	0.8802983173591912
1114	11	0.5563460668239131
1114	12	0.10207163356670179
1114	13	0.36286672252686003
1114	14	0.7690019522868212
1114	15	-1.4743305535556814
1114	16	0.29941456356532115
1114	17	0.6048902403378875
1114	18	0.6041140457873411
1114	19	1.2644809702230475
1114	20	1.1303176735094027
1114	21	0.9609567794135501
1114	22	1.1857037726676154
1114	23	0.492045127048776
1114	24	1.5942658149093276
1114	25	1.0504990455280279
1115	1	-3.5553358779660016
1115	2	-1.181277913122365
1115	3	-0.20359591163827062
1115	4	0.4791662632662508
1115	5	0.7937578567437242
1115	6	1.4093899910908405
1115	7	1.0854045011812765
1115	8	1.3028644561497522
1115	9	0.729999633821393
1115	10	-0.5614780578699055
1115	11	0.12975787404660366
1115	12	-0.02874066153642474
1115	13	0.4463933365334185
1115	14	0.5271816936327482
1115	15	0.8360159318982525
1115	16	0.7859352533095034
1115	17	0.5830043501497073
1115	18	0.37455081493470743
1115	19	-1.8224814578210484
1115	20	-0.2430892936296587
1115	21	0.17193473444594787
1115	22	0.1807488177126615
1115	23	0.21296751792102242
1115	24	-1.5706974649894825
1115	25	-0.4676619162933875
1116	1	0.29274047707756523
1116	2	-0.43512151081842276
1116	3	-0.11960905593010196
1116	4	0.1630940435055095
1116	5	0.5303089570243316
1116	6	1.3279526110878876
1116	7	0.9844205254022249
1116	8	-1.5554984149180413
1116	9	-0.3474316909459958
1116	10	-0.4556185097972474
1116	11	0.3453685667064761
1116	12	0.21086020982674114
1116	13	0.24155837862468843
1116	14	0.06593150902190814
1116	15	-0.567633824415438
1116	16	0.10031643777265487
1116	17	0.23998817824008967
1116	18	0.2658297988366414
1116	19	0.7481783413223582
1116	20	0.49218060741893055
1116	21	0.5093479673509833
1116	22	0.386250220755052
1116	23	0.49697098860482214
1116	24	1.3404696447432016
1116	25	0.6685568844745902
1201	1	0.5724457565890297
1201	2	0.5104036523276712
1201	3	0.8402691180641995
1201	4	0.4229241485266044
1201	5	0.6200614210398996
1201	6	0.7620992714479087
1201	7	1.2230910529688153
1201	8	1.226333051416372
1201	9	0.8460663074619603
1201	10	1.0117534248443363
1201	11	0.9235587121824196
1201	12	0.9849468031978361
1201	13	0.8027155718581156
1201	14	0.8906604961509643
1201	15	1.5098510530855904
1201	16	1.0731386248724846
1201	17	0.9654874225958281
1201	18	0.8948496147901608
1201	19	0.439942780280532
1201	20	1.1371581688222392
1201	21	1.1790045241512104
1201	22	1.3674067987056162
1201	23	1.121927630390985
1201	24	0.21131946573434135
1201	25	0.7248141188550576
1202	1	0.49263901045891956
1202	2	0.18718720330518934
1202	3	0.12009072680740474
1202	4	0.025645502959527278
1202	5	0.40489118177676175
1202	6	0.6986922717556325
1202	7	0.6580643775707777
1202	8	1.0624712457884335
1202	9	1.0647931002147928
1202	10	0.8981003192315029
1202	11	0.770751099731541
1202	12	1.0442256871652162
1202	13	0.5673281888201487
1202	14	0.7605866967932471
1202	15	1.29989569578688
1202	16	1.1837498822545385
1202	17	0.8871050933450633
1202	18	0.8969568141302204
1202	19	0.2467337974767787
1202	20	0.7092490741498878
1202	21	0.8847183011451776
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2210	24	0.9150697803580528
2210	25	0.6433026066236849
2211	1	0.24748059269898803
2211	2	0.1516700771128896
2211	3	-0.23899922382190653
2211	4	0.27302673508387443
2211	5	0.3637445226492204
2211	6	-2.02871079074108
2211	7	-0.1863463157189038
2211	8	0.23582766981603953
2211	9	0.35836883736410385
2211	10	0.4280497167363251
2211	11	0.3436144584523717
2211	12	0.23759428384951775
2211	13	0.2287298749426796
2211	14	0.3759987514872236
2211	15	0.9541657277411701
2211	16	-0.06802650869389737
2211	17	0.019018026771405516
2211	18	-0.059386290547358556
2211	19	0.35170007887558086
2211	20	0.01078622491846204
2211	21	-0.16631019424858806
2211	22	-2.9757929080807237
2211	23	-1.408776774651571
2211	24	0.571484283747786
2211	25	0.3051049287048997
2212	1	-0.5994921536796554
2212	2	-0.21466873954597787
2212	3	-3.1215996882486046
2212	4	-1.7756257066896524
2212	5	-0.5981063435221152
2212	6	0.035290628261972046
2212	7	0.5117038977675641
2212	8	0.4155008044418338
2212	9	0.33804840273081993
2212	10	0.36647360573749665
2212	11	0.18190252455190858
2212	12	0.13111782106796
2212	13	0.1334397213257077
2212	14	-0.20709737466270894
2212	15	-0.9313730906137181
2212	16	-0.0012058104708998485
2212	17	0.12991792062884855
2212	18	0.15255891671330346
2212	19	0.8961096449913697
2212	20	-0.5192948024171358
2212	21	-0.6627831801899973
2212	22	0.04963819889712795
2212	23	0.22468371303885704
2212	24	1.3926164050866845
2212	25	0.8175420523122199
2213	1	-0.6742893374565107
2213	2	-0.6849615939312628
2213	3	-0.05203718749195119
2213	4	-6.894177859144499
2213	5	-1.8817383243885857
2213	6	-3.720983525734429
2213	7	-1.498859293055233
2213	8	-3.1288144226141377
2213	9	-1.225704023598233
2213	10	-0.6242804495781016
2213	11	-0.5344715477933875
2213	12	-0.43352543625945117
2213	13	-0.5142223265161903
2213	14	-0.7945249210807575
2213	15	-2.968090191744057
2213	16	-1.3922947429130232
2213	17	-0.935650469230275
2213	18	-0.7864509392606109
2213	19	-0.09034326707429585
2213	20	-1.8146215884172676
2213	21	-0.7046189444483417
2213	22	-0.6958585478378367
2213	23	-0.5995861687351933
2213	24	-0.7602783080381599
2213	25	-0.4670749206245475
2214	1	0.3034419527281287
2214	2	0.15732390455434242
2214	3	0.1848630318867528
2214	4	0.4472229763670157
2214	5	0.5726526656495423
2214	6	0.40937758882073183
2214	7	0.9832668459048612
2214	8	-2.6906516370359483
2214	9	-0.9307114627377189
2214	10	-0.5596127564451336
2214	11	-0.03202725910416859
2214	12	-1.212123107859398
2214	13	-0.35438899866521467
2214	14	-0.11066209729474252
2214	15	-1.6612630100392778
2214	16	-0.2194622146430305
2214	17	-0.21232098619986317
2214	18	-0.18937048522006705
2214	19	0.8212236459611475
2214	20	-1.088172537666651
2214	21	-0.4309098502268327
2214	22	-0.09942838592269254
2214	23	-0.42719549751732444
2214	24	-1.7225632625050622
2214	25	-0.8340224949337056
2215	1	0.4401468413821945
2215	2	-0.011740263641442539
2215	3	0.32345849284725503
2215	4	0.6034142957395687
2215	5	0.6989506305548119
2215	6	1.3029382192217298
2215	7	0.8367396085739252
2215	8	-0.562436622887148
2215	9	0.028772576131615324
2215	10	-0.01759564222898291
2215	11	-0.16001974798385277
2215	12	-0.32387422703060764
2215	13	-0.170996642586708
2215	14	-0.10611550145537052
2215	15	-0.05653393157948727
2215	16	0.08604147784846816
2215	17	-0.20863950196821435
2215	18	-0.8857285081485031
2215	19	0.27223268500898
2215	20	-3.545430431363826
2215	21	-2.0439949310380143
2215	22	-0.9141341527780895
2215	23	-0.8404522916778774
2215	24	-0.699129685702907
2215	25	-0.5402349958559327
2216	1	-0.5589235518556919
2216	2	-1.0764760927165185
2216	3	-0.3453476918128686
2216	4	-0.47779618446800853
2216	5	-0.5806465231136784
2216	6	-1.348013546933501
2216	7	-10.016642467056965
2216	8	-11.249358843556774
2216	9	-5.844472069425478
2216	10	-3.214880621507369
2216	11	-2.167739416739277
2216	12	-1.6270063575370202
2216	13	-1.5113833288774785
2216	14	-1.4814813964061813
2216	15	-2.537053607698134
2216	16	-1.6821221820298078
2216	17	-2.002282698644059
2216	18	-1.5689707913385718
2216	19	-1.08195585865451
2216	20	-2.1155861774520446
2216	21	-4.670002388531444
2216	22	-3.1362373734346
2216	23	-3.0456708636200425
2216	24	-3.418876535903454
2216	25	-2.7385452479988714
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53204.0
3	10.0
4	402.0
5	2497.0
6	3373.0
7	2695.0
8	2130.0
9	1953.0
10	1844.0
11	1715.0
12	1763.0
13	1762.0
14	1807.0
15	1855.0
16	1963.0
17	2078.0
18	2430.0
19	2895.0
20	3857.0
21	5031.0
22	6254.0
23	7495.0
24	8725.0
25	10089.0
26	11669.0
27	12790.0
28	13963.0
29	16946.0
30	20236.0
31	24576.0
32	31643.0
33	42106.0
34	57504.0
35	80071.0
36	116875.0
37	193460.0
38	293285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.817102580689195	18.95478675600573	12.180729944126238	26.047380719178843
2	13.724174153845563	20.539307329129567	43.10550608912408	22.631012427900796
3	18.511334567550335	25.008738102737045	30.11034975456494	26.369577575147684
4	11.426638842915738	17.016334460674965	39.872010745724495	31.685015950684804
5	11.900695195012318	38.51010185772142	37.50888294082163	12.080320006444628
6	30.193606423114623	38.50090243532867	19.54544495528102	11.760046186275694
7	26.90619925972097	31.226866280367215	23.597740686874282	18.26919377303753
8	23.367476792291125	38.77185506808652	22.021040117041302	15.839628022581046
9	25.782439746673262	15.441653144911024	20.843930170201503	37.931976938214206
10	13.475018127827077	29.978534350793595	36.282318366845836	20.264129154533492
11	33.84950724059529	22.571900103663115	25.761003297848774	17.817589357892828
12	22.783529474943993	25.088800748764843	33.339502715806915	18.78816706048424
13	28.78557585440067	22.81774096261341	26.090254476619357	22.306428706366567
14	19.2853498737984	23.322554412452913	28.534507890476956	28.85758782327173
15	21.913270957057414	33.16871417735332	23.365211912801072	21.552802952788184
16	20.460911869377295	28.959519889063536	29.85886480001611	20.72070344154306
17	20.377726162916705	29.78626077183184	29.146232232351267	20.68978083290019
18	20.10118823293623	28.471081473213218	31.578841379389704	19.848888914460847
19	23.00028962546249	27.508472983314885	27.85391368393998	21.63732370728264
20	22.546481163956624	28.49898541218349	29.17448225334392	19.780051170515964
21	21.76327930288155	27.803131356041916	27.635410962910683	22.798178378165854
22	21.848523526811274	29.732572936615178	28.13253687946459	20.286366657108964
23	20.860256506153732	29.153936297327725	28.788567505001044	21.197239691517495
24	22.601596857684804	27.70450269371441	29.481644575459487	20.2122558731413
25	21.501814816768235	28.42276360393362	29.164704810582997	20.910716768715147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	311.0
1	311.0
2	385.5
3	460.0
4	460.0
5	460.0
6	777.5
7	1095.0
8	1095.0
9	1095.0
10	1685.0
11	2275.0
12	2275.0
13	2275.0
14	4003.5
15	5732.0
16	5732.0
17	5732.0
18	9875.5
19	14019.0
20	14019.0
21	14019.0
22	22428.0
23	30837.0
24	30837.0
25	30837.0
26	44634.5
27	58432.0
28	58432.0
29	58432.0
30	76362.5
31	94293.0
32	94293.0
33	94293.0
34	105323.0
35	116353.0
36	116353.0
37	116353.0
38	123088.5
39	129824.0
40	129824.0
41	129824.0
42	133288.5
43	136753.0
44	136753.0
45	136753.0
46	136643.5
47	136534.0
48	136534.0
49	136534.0
50	126904.5
51	117275.0
52	117275.0
53	117275.0
54	100265.5
55	83256.0
56	83256.0
57	83256.0
58	69459.0
59	55662.0
60	55662.0
61	55662.0
62	44000.5
63	32339.0
64	32339.0
65	32339.0
66	24490.0
67	16641.0
68	16641.0
69	16641.0
70	11816.5
71	6992.0
72	6992.0
73	6992.0
74	4787.0
75	2582.0
76	2582.0
77	2582.0
78	1742.5
79	903.0
80	903.0
81	903.0
82	586.5
83	270.0
84	270.0
85	270.0
86	175.5
87	81.0
88	81.0
89	81.0
90	51.0
91	21.0
92	21.0
93	21.0
94	13.5
95	6.0
96	6.0
97	6.0
98	5.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2464161787082998
2	0.14420619952423458
3	0.14717853475378997
4	0.06481608436062672
5	0.021285755514880374
6	0.02080634660688757
7	0.32053279588398687
8	0.3181357513440229
9	0.017929893158930763
10	0.034038032467488884
11	0.014382267239784037
12	0.016012257526959558
13	0.013806976550192673
14	0.016779311779748042
15	0.013423449423798434
16	0.018409302066923566
17	0.017738129595733645
18	0.013615212986995553
19	0.021573400859676056
20	0.015628730400565317
21	0.013806976550192673
22	0.015532848618966758
23	0.01620402109015668
24	0.014669912584579716
25	0.013998740113389797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1042951.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.47835127287074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.56610971265586	79.04310683541705
2	4.89882011765889	8.276884934443874
3	0.7443008241496628	1.8863191942560718
4	0.25783737222798786	0.8712670440939951
5	0.13247364676805098	0.5595577633034804
6	0.08077258749653993	0.4094121011850832
7	0.06295513383429809	0.3722842137339095
8	0.03566744950783711	0.24105018628243544
9	0.029243337752039675	0.22233860631072086
>10	0.16107081757423816	2.5638125863838157
>50	0.014757882263571524	0.8783127107379296
>100	0.012815581379133585	2.1038633102562763
>500	0.0023816525489179356	1.230565784622375
>1k	7.938841829726451E-4	1.3412247289729544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	2927	0.28064597473898584	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2328	0.22321278756144824	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2257	0.21640518106795045	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1997	0.1914759178523248	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1859	0.17824423199172348	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1347	0.12915275981326063	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1284	0.12311220757255134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	9.588178159856025E-5	0.0	0.0
7	0.0	0.0	9.588178159856025E-5	0.0	0.0
8	0.0	0.0	9.588178159856025E-5	0.0	0.0
9	0.0	0.0	9.588178159856025E-5	0.0	0.0
10	0.0	0.0	9.588178159856025E-5	0.0	0.0
11	0.0	0.0	9.588178159856025E-5	9.588178159856025E-5	0.0
12	0.0	0.0	9.588178159856025E-5	9.588178159856025E-5	0.0
13	0.0	0.0	9.588178159856025E-5	9.588178159856025E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCAC	35	0.0021996356	16.252174	13
TAGTACG	40	0.005187263	14.287107	4
CTAGCAC	70	6.980692E-6	13.62252	3
CGCCACC	50	0.0015218856	13.273246	14
TAGGACC	535	0.0	13.174404	4
AACACGG	55	0.0030211504	12.115391	5
AGGACCT	900	0.0	11.951928	5
TCGAACT	80	2.9238667E-5	11.851112	19
CTGTGCG	65	8.1495737E-4	11.669908	9
CCAACGT	65	8.1561337E-4	11.668788	19
GTCCTAC	1525	0.0	11.64286	1
ATGTCCA	385	0.0	11.575184	9
TGGCGAG	1285	0.0	11.436093	18
TCCTACA	1650	0.0	11.386223	2
GGCGAGG	560	0.0	11.343207	19
CCCTTAC	85	5.063843E-5	11.230462	1
GGACCTG	920	0.0	11.174755	6
CTAGAGC	60	0.005711412	11.125058	3
AATCCCG	145	1.2551027E-9	11.115526	19
CTACTAG	70	0.0014319252	10.909593	1
>>END_MODULE