FastQCFastQC Report
Thu 26 May 2016
SRR1926817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042951
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28500.2732630775558967No Hit
CTGTAGGACGTGGAATATGGCAAGA27500.26367489939604066No Hit
GTCCTAAAGTGTGTATTTCTCATTT23310.22350043290624394No Hit
CTTTAGGACGTGAAATATGGCGAGG22420.21496695434397206No Hit
GTCCTACAGTGTGCATTTCTCATTT20390.19550295267946433No Hit
GTATCAACGCAGAGTACTTTTTTTT19360.1856271291748126No Hit
CTGTAGGACCTGGAATATGGCGAGA15970.1531232052129007No Hit
CTGAAGGACCTGGAATATGGCGAGA13110.12570101567571246No Hit
GGTATCAACGCAGAGTACTTTTTTT12130.11630460107905356No Hit
TATCAACGCAGAGTACTTTTTTTTT10900.10451114194243065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6050.014.3225154
CGATCTT400.005322484814.23180514
AGGACCT9750.012.7938125
GGACCTG9700.012.6628176
GGCGAGG6350.012.55268819
ATAGGAC1850.012.3547943
TAGGACT2000.011.9026394
TGGCGAG15100.011.5630418
TGTCCAC3800.011.48531510
CCCTAAT752.0093183E-411.4380921
TGTAGGA16150.011.3307652
CTGTAGG16000.011.3189451
AATGTCC3700.011.281798
GATATAC3150.011.1960161
GACAGCG600.00592535211.0702427
GTCCTAC13700.011.0623881
TAGAAAT4150.011.0135274
GTAGGAC15800.010.9098173
CTAGACA1053.3669003E-610.8824144
ATGGCGA16300.010.82820617
Produced by FastQC (version 0.11.5)