##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1042951 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.911313187292595 31.0 30.0 33.0 26.0 34.0 2 30.21025052950714 31.0 30.0 34.0 26.0 34.0 3 30.283788979539786 31.0 30.0 34.0 26.0 34.0 4 33.864758747055234 37.0 35.0 37.0 30.0 37.0 5 33.830362116724565 37.0 35.0 37.0 30.0 37.0 6 33.82453921612808 37.0 35.0 37.0 30.0 37.0 7 33.819022178414905 37.0 35.0 37.0 29.0 37.0 8 33.8576251425043 37.0 35.0 37.0 30.0 37.0 9 35.399373508439034 39.0 35.0 39.0 30.0 39.0 10 35.294686902836276 39.0 35.0 39.0 30.0 39.0 11 35.32898669256753 39.0 35.0 39.0 30.0 39.0 12 35.22189249542883 39.0 35.0 39.0 29.0 39.0 13 35.22836068041548 39.0 35.0 39.0 29.0 39.0 14 36.34156158822418 40.0 37.0 41.0 29.0 41.0 15 36.34544767683237 40.0 37.0 41.0 29.0 41.0 16 36.33881265754575 40.0 37.0 41.0 29.0 41.0 17 36.318476131668696 40.0 37.0 41.0 29.0 41.0 18 36.28871538547832 40.0 37.0 41.0 29.0 41.0 19 36.35424962438312 40.0 37.0 41.0 29.0 41.0 20 36.322393861264814 40.0 37.0 41.0 29.0 41.0 21 36.27341265313519 40.0 37.0 41.0 29.0 41.0 22 36.17692969276601 40.0 37.0 41.0 29.0 41.0 23 36.112943944634026 40.0 37.0 41.0 29.0 41.0 24 36.047055901955126 40.0 37.0 41.0 29.0 41.0 25 35.93672952995874 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24885929922333716 1101 2 -0.1586936329960409 1101 3 0.028147243584420778 1101 4 -0.13036872353617923 1101 5 -0.20356667935329398 1101 6 -0.05806478293629169 1101 7 -0.016021706800430024 1101 8 0.09987172747968742 1101 9 0.1505371898670873 1101 10 0.06567286695029395 1101 11 0.0397884817944032 1101 12 -0.10707309346624072 1101 13 0.030677496748971578 1101 14 0.02640448644386595 1101 15 0.03328251440240848 1101 16 -0.04940477799627274 1101 17 -0.06266064341411237 1101 18 -0.2103978878646231 1101 19 -0.22827142808696976 1101 20 -0.23305721162100212 1101 21 -0.1459060087508206 1101 22 -0.14264430523219573 1101 23 -0.10846048355085003 1101 24 -0.17544178308130398 1101 25 -0.15742871423655913 1102 1 -1.0215412750187305 1102 2 -1.0789289450030353 1102 3 -0.7856668148552863 1102 4 -0.9289225295028771 1102 5 -0.9758549297734902 1102 6 -0.8800724022011437 1102 7 -0.6144829194202757 1102 8 -0.638633528075232 1102 9 -0.7105972279760095 1102 10 -0.6697301041828467 1102 11 -0.9001453180356123 1102 12 -0.7109690739374628 1102 13 -0.7304289530157391 1102 14 -0.8317932831697732 1102 15 -0.7526259186520576 1102 16 -0.7292911135534723 1102 17 -0.8459243280862481 1102 18 -0.7004827967782958 1102 19 -0.7525737874523415 1102 20 -0.8426038300630978 1102 21 -0.9569870700172061 1102 22 -0.9650632996304296 1102 23 -0.8973938136257686 1102 24 -0.7774872542891131 1102 25 -0.6082388381378578 1103 1 -0.3314508594025334 1103 2 -0.4397743022720064 1103 3 -0.3268876309773532 1103 4 -0.16165719812951096 1103 5 -0.15265947820182646 1103 6 -0.2649300448254124 1103 7 -0.2618360111525746 1103 8 -0.18993200362907459 1103 9 -0.16089053809636766 1103 10 -0.2743020739731392 1103 11 -0.09542169746914198 1103 12 -0.12778424339489192 1103 13 -0.1094710347374459 1103 14 -0.15601525172824182 1103 15 -0.17741507206297058 1103 16 -0.2311415815083535 1103 17 -0.13640126156521148 1103 18 -0.16852800732377204 1103 19 -0.09756698506644312 1103 20 -0.14051548390514768 1103 21 -0.08687705477000662 1103 22 -0.10665147196484526 1103 23 -0.04440747040776216 1103 24 -0.16032471148335503 1103 25 -0.21476529627102536 1104 1 -0.5525524257242793 1104 2 -0.472490392044957 1104 3 -0.264221407647085 1104 4 -0.4538425928658256 1104 5 -0.44057397659295106 1104 6 -0.4728824137596348 1104 7 -0.3346709293245169 1104 8 -0.4403218147259267 1104 9 -0.5419773443427829 1104 10 -0.46608913362458537 1104 11 -0.457505574401452 1104 12 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1107 9 0.27658783576408297 1107 10 0.19438026710455603 1107 11 0.25816208429093024 1107 12 0.3911154444149929 1107 13 0.33163816646764843 1107 14 0.4105696170878659 1107 15 0.4783440682987887 1107 16 0.3466895246281183 1107 17 0.2608779605342235 1107 18 0.2960708887706076 1107 19 0.38967869690540624 1107 20 0.3626582125195199 1107 21 0.41104919993293976 1107 22 0.36555982525106856 1107 23 0.2461580093518947 1107 24 0.2131273942202725 1107 25 0.05499266719890983 1108 1 0.5347944154006221 1108 2 0.38458126152974614 1108 3 0.5075593569734487 1108 4 0.506014056041991 1108 5 0.5596156283371059 1108 6 0.5737400551569536 1108 7 0.3931788612556204 1108 8 0.3764840176193758 1108 9 0.3293281059445121 1108 10 0.5018859426852984 1108 11 0.4362106903651508 1108 12 0.4754484055318642 1108 13 0.4102880815598553 1108 14 0.42166853053535647 1108 15 0.37794463628906527 1108 16 0.46111072540733744 1108 17 0.45706131420718066 1108 18 0.35215128438353105 1108 19 0.31073724280943793 1108 20 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0.34924035012260646 2214 8 0.3365369463996686 2214 9 0.17350539557711642 2214 10 0.16141693488211217 2214 11 0.3074166443084039 2214 12 0.40501079015856334 2214 13 0.37226594077583286 2214 14 0.41474000617014894 2214 15 0.192105127023801 2214 16 0.3217561080247009 2214 17 0.3792112191858621 2214 18 0.3193008626413487 2214 19 0.2807980401172614 2214 20 0.2517769032000814 2214 21 0.35061829123411314 2214 22 0.26962372290727643 2214 23 0.3943991854765301 2214 24 0.1998152658200354 2214 25 0.22850472782550924 2215 1 0.42028577109049436 2215 2 0.30405585148513126 2215 3 0.2787671162509966 2215 4 0.15718914664547867 2215 5 0.36544870957672515 2215 6 0.32638236456242 2215 7 0.22913263361265734 2215 8 0.1995970377421301 2215 9 0.12193010706774743 2215 10 0.21083122933421095 2215 11 0.015626220272821456 2215 12 0.15276144047973617 2215 13 -0.06635635411417695 2215 14 0.08095002004525753 2215 15 0.08387782043634218 2215 16 0.04927808090755548 2215 17 0.09178086245525208 2215 18 -0.028208044319285364 2215 19 -0.10096518109665453 2215 20 0.08024561995516422 2215 21 0.09984510517205081 2215 22 0.09452370553364631 2215 23 -0.0987534025994492 2215 24 0.07591182750713443 2215 25 -0.05098671268646626 2216 1 0.12457905937429814 2216 2 0.28298448596726544 2216 3 -1.3978089409500782 2216 4 -0.9761378833236733 2216 5 -0.11012840385622269 2216 6 -0.07314995928435053 2216 7 -0.005116835133669895 2216 8 0.10092121836753876 2216 9 0.10520583155718555 2216 10 -0.05422774477374048 2216 11 0.07873321541727307 2216 12 0.061821187725989546 2216 13 0.04662726522733607 2216 14 0.017553237494304597 2216 15 0.08763166904459752 2216 16 0.1460768365230578 2216 17 0.08747784021525007 2216 18 0.2597106657111752 2216 19 0.08109466348916072 2216 20 0.07189589369130545 2216 21 0.047971764860385235 2216 22 0.04750813929211972 2216 23 0.030347584267516936 2216 24 0.012489106333660516 2216 25 -0.0582564997415318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24911.0 3 18.0 4 380.0 5 2799.0 6 3593.0 7 2744.0 8 2033.0 9 1750.0 10 1602.0 11 1442.0 12 1373.0 13 1275.0 14 1265.0 15 1254.0 16 1305.0 17 1334.0 18 1518.0 19 1852.0 20 2466.0 21 3079.0 22 3934.0 23 4836.0 24 5628.0 25 6791.0 26 8011.0 27 8772.0 28 10284.0 29 12508.0 30 15888.0 31 20436.0 32 26719.0 33 35136.0 34 48108.0 35 67294.0 36 99829.0 37 176135.0 38 434649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.408615383433904 19.80526399659025 10.302404297516219 28.483716322459628 2 13.618075642215793 21.407705149534355 41.18033882229158 23.79388038595828 3 18.033777310646347 25.36625970610997 29.617912274123466 26.98205070912022 4 11.558644653488036 17.94408366260735 38.98198477205545 31.515286911849167 5 12.073791312002163 39.20846649732905 36.33183652687204 12.38590566379675 6 30.68921627011806 39.29261025545806 18.146921332618838 11.871252141805053 7 27.354124188886182 31.94901323676372 22.715886226361896 17.980976347988207 8 23.845132212979166 39.84062522475008 20.23234962721454 16.081892935056217 9 25.918925683314775 15.676515912126817 20.185439303581344 38.21911910097706 10 13.65498738614691 30.521209587487213 35.43135925653538 20.3924437698305 11 33.93755231860725 22.82390551631094 25.378827468695135 17.85971469638667 12 23.018419990354218 25.271563258970048 32.78798553708113 18.922031213594607 13 29.016462132492887 22.86768449544988 25.814836725829604 22.30101664622763 14 19.616683078463087 23.557815386946054 28.079225350424615 28.74627618416624 15 21.587870198932034 33.397895713856975 23.29289235309741 21.72134173411358 16 20.621300725544398 29.215842506910743 29.483738773334256 20.679117994210603 17 20.30532682358085 30.125471260312114 28.862321036139864 20.706880879967173 18 20.215927014367967 28.672844685002563 31.307787083690897 19.803441216938573 19 22.790537573373886 27.6689301267755 27.880158704285744 21.66037359556487 20 22.63280220760119 28.741483227255205 28.849255279775864 19.77645928536774 21 21.605249631766295 27.70747667853197 27.636706763225728 23.050566926476005 22 21.987535356440866 29.794621026894863 27.68675391917158 20.53108969749269 23 20.76446617766911 29.48108730044585 28.562634833884655 21.191811688000385 24 22.432736404177003 27.71755857668975 29.43816044696337 20.411544572169877 25 21.48882289711964 28.72639614302933 28.9320743970367 20.85270656281433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 339.0 1 339.0 2 337.5 3 336.0 4 336.0 5 336.0 6 623.0 7 910.0 8 910.0 9 910.0 10 1552.0 11 2194.0 12 2194.0 13 2194.0 14 3764.5 15 5335.0 16 5335.0 17 5335.0 18 9345.5 19 13356.0 20 13356.0 21 13356.0 22 21480.5 23 29605.0 24 29605.0 25 29605.0 26 42846.0 27 56087.0 28 56087.0 29 56087.0 30 73790.5 31 91494.0 32 91494.0 33 91494.0 34 103073.5 35 114653.0 36 114653.0 37 114653.0 38 121886.0 39 129119.0 40 129119.0 41 129119.0 42 133050.5 43 136982.0 44 136982.0 45 136982.0 46 137555.5 47 138129.0 48 138129.0 49 138129.0 50 129378.0 51 120627.0 52 120627.0 53 120627.0 54 102998.0 55 85369.0 56 85369.0 57 85369.0 58 71201.5 59 57034.0 60 57034.0 61 57034.0 62 45061.0 63 33088.0 64 33088.0 65 33088.0 66 25085.5 67 17083.0 68 17083.0 69 17083.0 70 12152.5 71 7222.0 72 7222.0 73 7222.0 74 4957.0 75 2692.0 76 2692.0 77 2692.0 78 1805.0 79 918.0 80 918.0 81 918.0 82 598.5 83 279.0 84 279.0 85 279.0 86 178.5 87 78.0 88 78.0 89 78.0 90 49.0 91 20.0 92 20.0 93 20.0 94 11.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11822223671102478 2 0.12311220757255134 3 0.017258720687740845 4 0.0 5 0.006136434022307856 6 0.33788739835332626 7 0.023874563618041498 8 0.01208110448141859 9 0.012464631607812832 10 0.004794089079928013 11 0.005944670459110735 12 7.67054252788482E-4 13 0.001150581379182723 14 1.917635631971205E-4 15 0.003068217011153928 16 9.588178159856024E-4 17 2.876453447956807E-4 18 5.752906895913614E-4 19 4.794089079928012E-4 20 8.629360343870421E-4 21 0.012944040515805633 22 9.588178159856025E-5 23 9.588178159856025E-5 24 1.917635631971205E-4 25 0.0057529068959136145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1042951.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.99002814998708 #Duplication Level Percentage of deduplicated Percentage of total 1 92.05903819729217 75.47923133256721 2 6.099396025442857 10.001793036479583 3 0.9456302948783851 2.3259676348967813 4 0.2968286953118216 0.9734797233736078 5 0.149583271473829 0.61321683194532 6 0.09560667770384541 0.47032765177590174 7 0.06407427923694728 0.36774163708292085 8 0.042984827387354686 0.28194617660092375 9 0.030455966194945713 0.22473769730927887 >10 0.18067478544292728 2.7550256533709256 >50 0.017227580902209258 0.9812833059560994 >100 0.014986857967648004 2.441224893185962 >500 0.002341693843362377 1.2256035559917267 >1k 0.0011708469216811885 1.858420869463761 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2850 0.2732630775558967 No Hit CTGTAGGACGTGGAATATGGCAAGA 2750 0.26367489939604066 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2331 0.22350043290624394 No Hit CTTTAGGACGTGAAATATGGCGAGG 2242 0.21496695434397206 No Hit GTCCTACAGTGTGCATTTCTCATTT 2039 0.19550295267946433 No Hit GTATCAACGCAGAGTACTTTTTTTT 1936 0.1856271291748126 No Hit CTGTAGGACCTGGAATATGGCGAGA 1597 0.1531232052129007 No Hit CTGAAGGACCTGGAATATGGCGAGA 1311 0.12570101567571246 No Hit GGTATCAACGCAGAGTACTTTTTTT 1213 0.11630460107905356 No Hit TATCAACGCAGAGTACTTTTTTTTT 1090 0.10451114194243065 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.588178159856025E-5 0.0 9 0.0 0.0 0.0 9.588178159856025E-5 0.0 10 0.0 0.0 0.0 9.588178159856025E-5 0.0 11 0.0 0.0 0.0 1.917635631971205E-4 0.0 12 0.0 0.0 0.0 1.917635631971205E-4 0.0 13 0.0 0.0 0.0 1.917635631971205E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 605 0.0 14.322515 4 CGATCTT 40 0.0053224848 14.231805 14 AGGACCT 975 0.0 12.793812 5 GGACCTG 970 0.0 12.662817 6 GGCGAGG 635 0.0 12.552688 19 ATAGGAC 185 0.0 12.354794 3 TAGGACT 200 0.0 11.902639 4 TGGCGAG 1510 0.0 11.56304 18 TGTCCAC 380 0.0 11.485315 10 CCCTAAT 75 2.0093183E-4 11.438092 1 TGTAGGA 1615 0.0 11.330765 2 CTGTAGG 1600 0.0 11.318945 1 AATGTCC 370 0.0 11.28179 8 GATATAC 315 0.0 11.196016 1 GACAGCG 60 0.005925352 11.070242 7 GTCCTAC 1370 0.0 11.062388 1 TAGAAAT 415 0.0 11.013527 4 GTAGGAC 1580 0.0 10.909817 3 CTAGACA 105 3.3669003E-6 10.882414 4 ATGGCGA 1630 0.0 10.828206 17 >>END_MODULE