FastQCFastQC Report
Thu 26 May 2016
SRR1926816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992977
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22470.2262892292570724No Hit
GTCCTAAAGTGTGTATTTCTCATTT16510.1662676980433585No Hit
CTGTAGGACGTGGAATATGGCAAGA16150.16264223642642278No Hit
GTCCTACAGTGTGCATTTCTCATTT15680.15790899487097887No Hit
CTTTAGGACGTGAAATATGGCGAGG12660.1274954001955735No Hit
GTATCAACGCAGAGTACTTTTTTTT12440.1252798403185572No Hit
GTACATGGGAAGCAGTGGTATCAAC10200.10272141247984595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG250.00596737819.034685
GCACCGT400.005213847414.276016
GGCGAGG3500.014.08521919
ATCCCGT852.759116E-713.38616810
TCCAACG1153.8926373E-1013.19017218
GTTCTAA1102.5484042E-913.0176761
CCCGTTT905.535003E-712.64058112
CCAACGA854.0310915E-612.26879919
TAGGACC5300.012.221434
GCCTTAC550.002957404812.1498311
GTCTTAG1353.4197E-1012.021261
ACGAAAT658.1588095E-411.66822912
CGTTTCC907.62584E-611.587214
GCTAGAC751.9930172E-411.4480083
GTCCTAC10350.011.4371081
CGAAATC752.112433E-411.37652413
TCCCGTT1001.975457E-611.37652311
TAGATTG855.2202216E-511.1968715
TGGCGAG10200.011.15345518
CCCTAAT600.00566433311.1373451
Produced by FastQC (version 0.11.5)