##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926816_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992977 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.720886787911503 31.0 30.0 33.0 23.0 34.0 2 28.791323464692535 31.0 30.0 33.0 23.0 34.0 3 28.574560135833963 31.0 29.0 33.0 16.0 34.0 4 32.15895131508585 35.0 32.0 37.0 22.0 37.0 5 32.41502069030803 35.0 33.0 37.0 25.0 37.0 6 31.60093637616984 35.0 32.0 37.0 17.0 37.0 7 31.65795179545951 35.0 32.0 37.0 17.0 37.0 8 31.481627469719843 35.0 32.0 37.0 17.0 37.0 9 33.709570312303306 37.0 34.0 39.0 22.0 39.0 10 33.87558523510615 38.0 34.0 39.0 23.0 39.0 11 34.046361597499235 38.0 35.0 39.0 25.0 39.0 12 33.921410062871544 38.0 35.0 39.0 23.0 39.0 13 34.03514280794016 38.0 35.0 39.0 25.0 39.0 14 35.09221764451745 39.0 36.0 41.0 25.0 41.0 15 34.677359092909505 39.0 34.0 41.0 19.0 41.0 16 34.77757490858298 39.0 34.0 41.0 22.0 41.0 17 35.01292174944636 39.0 35.0 41.0 25.0 41.0 18 35.06365102112134 39.0 36.0 41.0 25.0 41.0 19 34.46382746025336 39.0 34.0 41.0 18.0 41.0 20 34.73180043445115 39.0 34.0 41.0 19.0 41.0 21 34.71810726733852 39.0 35.0 41.0 19.0 41.0 22 34.74883607576006 39.0 35.0 41.0 19.0 41.0 23 34.75225609455204 39.0 35.0 41.0 19.0 41.0 24 33.48014908703827 39.0 33.0 41.0 2.0 41.0 25 34.0642502293608 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.21185945909696358 1101 2 -0.2678263797929681 1101 3 -1.1290785319974432 1101 4 -0.5568700672493989 1101 5 -0.2164320860832376 1101 6 -2.248767313382057 1101 7 -0.5558787196366595 1101 8 -0.31056267407830873 1101 9 -0.24911350596273962 1101 10 -9.512085625290116 1101 11 -3.883379435437149 1101 12 -1.6191973795743735 1101 13 -0.8412666927291639 1101 14 -0.6236417925724282 1101 15 -2.108361956506471 1101 16 -0.40830124497139053 1101 17 -0.26166373682338673 1101 18 -0.2352489026059601 1101 19 -0.9259097605858031 1101 20 -0.22548709167372039 1101 21 -0.321259625802945 1101 22 -0.021709201541760592 1101 23 -0.24217785463122254 1101 24 -0.2819604777330653 1101 25 -0.20697748522120918 1102 1 -0.10181897597608369 1102 2 -0.17395405218080384 1102 3 -0.2208346964726715 1102 4 -0.27444174913905783 1102 5 -0.048903898455684214 1102 6 -0.9590286135259234 1102 7 -0.1848642996159171 1102 8 0.37333441180613036 1102 9 0.1675866178286256 1102 10 0.27979433054647984 1102 11 0.1289495150128488 1102 12 0.12698014392925927 1102 13 -0.010166622469199638 1102 14 -0.1744882578272069 1102 15 0.20203651784864718 1102 16 0.2105133691006742 1102 17 0.1622296687773357 1102 18 -0.18293868814578929 1102 19 0.41618097513637053 1102 20 0.2776829708911066 1102 21 0.1876148765223249 1102 22 0.08026811452769778 1102 23 -0.4775069054525929 1102 24 -3.81520849539843 1102 25 -2.0844073089021578 1103 1 -0.3033021196093415 1103 2 -0.3394147250161055 1103 3 -0.14051728151087417 1103 4 -0.30963221025544385 1103 5 -0.34627125547561377 1103 6 -0.08368626161851012 1103 7 -0.15985825330538006 1103 8 -0.05309903068762267 1103 9 -0.1794576000776047 1103 10 -0.12777555686608366 1103 11 -0.39722750788656214 1103 12 -0.4712703012566166 1103 13 -0.31679020392070356 1103 14 -0.24757111049052583 1103 15 0.15175175763271653 1103 16 0.10212239738620355 1103 17 -0.1857898960563844 1103 18 -0.38164939996695324 1103 19 -1.6864279494981176 1103 20 -0.6034708425799806 1103 21 -0.3783818869506703 1103 22 -0.44082347096900065 1103 23 -0.44378016507229745 1103 24 1.0613823379279026 1103 25 0.3118165250470071 1104 1 -0.28378847575732635 1104 2 -0.2825510735672161 1104 3 -1.407026257529992 1104 4 -0.6264536221544006 1104 5 -0.36698249752127055 1104 6 -2.0184935878531647 1104 7 -0.44849777900325094 1104 8 0.022401525422079516 1104 9 -0.0821037458449112 1104 10 -0.19822958383017664 1104 11 -0.2712130035907663 1104 12 -0.224257985136596 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-0.2511531991217524 2214 22 0.2081399477135406 2214 23 -0.15826334627345062 2214 24 -1.44250729177935 2214 25 -0.426844754399184 2215 1 0.5585616177553163 2215 2 0.08796214861118301 2215 3 0.4567347317955708 2215 4 0.7905582832133504 2215 5 0.8161170012000909 2215 6 1.4955138263633039 2215 7 0.9596288352030413 2215 8 -0.3890751632126346 2215 9 0.19989292396004998 2215 10 -0.005711628767130605 2215 11 -0.10529486213858519 2215 12 -0.3264137854648439 2215 13 0.07220099147598091 2215 14 0.18628719704765473 2215 15 0.3072756668508987 2215 16 0.48533818080965574 2215 17 0.1495637009226911 2215 18 -0.5764119132926666 2215 19 0.5850629729622909 2215 20 -3.265346540975756 2215 21 -1.715736347692804 2215 22 -0.8225557654319289 2215 23 -0.6896311322300335 2215 24 -0.7189155961050062 2215 25 -0.25236564547073215 2216 1 -0.8165965238086486 2216 2 -1.1795131758516817 2216 3 -0.4805157862543794 2216 4 -0.6280403916694048 2216 5 -0.8730489383789255 2216 6 -1.8775891565137854 2216 7 -10.464180331554513 2216 8 -11.436027529882939 2216 9 -6.3072827495048855 2216 10 -3.6794744436869706 2216 11 -2.6336621868195778 2216 12 -2.276789371679264 2216 13 -2.1192034692067523 2216 14 -2.2832412411680707 2216 15 -3.4534643266053138 2216 16 -2.347635016921828 2216 17 -2.593497156313653 2216 18 -2.4251058821006097 2216 19 -1.6737949995537988 2216 20 -2.625935890959397 2216 21 -5.043819426545458 2216 22 -3.7327388551908953 2216 23 -3.495104057303653 2216 24 -4.066199242086029 2216 25 -3.232785402259914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47549.0 3 4.0 4 373.0 5 2278.0 6 2989.0 7 2459.0 8 1935.0 9 1759.0 10 1742.0 11 1672.0 12 1521.0 13 1567.0 14 1604.0 15 1750.0 16 1746.0 17 1844.0 18 2267.0 19 2794.0 20 3515.0 21 4677.0 22 6007.0 23 7040.0 24 8448.0 25 9473.0 26 11120.0 27 12059.0 28 13451.0 29 15889.0 30 19419.0 31 23248.0 32 30551.0 33 40395.0 34 55027.0 35 77984.0 36 113938.0 37 187545.0 38 275338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10632528813025 18.794158352774197 12.380944689691514 25.718571669404035 2 13.880514366578478 20.47722841583849 42.62759056839948 23.014666649183553 3 18.75457055217818 24.562513049714497 29.867127428759904 26.81578896934742 4 11.610050178330097 16.948867283910182 39.43468502742726 32.00639751033246 5 12.21918685821694 38.22141100104559 37.11649149527269 12.442910645464783 6 30.562477965248046 38.00221606648199 19.432082598841603 12.003223369428355 7 27.246285446994058 31.38055995869969 22.93359519833344 18.439559395972815 8 23.73686300142344 38.20733701938765 21.78435875080946 16.27144122837945 9 25.906187234711208 15.653121619362079 20.611390571583403 37.829300574343314 10 13.481419841268242 30.070077310707642 35.96952704867331 20.478975799350806 11 34.07858648673422 22.90895502393694 24.97328309245309 18.03917539687576 12 22.60218090689143 25.184478614985125 32.91314043743037 19.30020004069307 13 28.643158379271828 22.568298360315588 26.04861136991558 22.739931890497004 14 19.673788997303568 23.05556143123491 28.36351585879433 28.907133712667193 15 22.397290145957832 32.35366697521507 23.364936881874982 21.88410599695212 16 20.851993094125774 29.087973343486663 29.202198275948906 20.85783528643866 17 20.689168907825422 29.555092214869205 28.693278538260863 21.062460339044513 18 20.401945162375533 28.66576284570648 30.7109431499302 20.221348841987787 19 22.845138466373605 27.63696546499962 27.906524620945028 21.61137144768175 20 22.440962726819468 28.570766666163045 28.91615171156471 20.072118895452785 21 21.97966010191101 27.749748949212705 27.72537410848013 22.54521684039616 22 21.756069652943975 29.363179990491616 28.16002691356234 20.720723443002072 23 21.24869686088266 28.876354131517584 28.399216353829342 21.475732653770415 24 22.329649210984076 27.831356974433408 29.092916231458098 20.746077583124418 25 21.713034448234982 28.260626516508985 28.74661445192144 21.27972458333459 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 337.0 1 337.0 2 417.5 3 498.0 4 498.0 5 498.0 6 746.5 7 995.0 8 995.0 9 995.0 10 1509.5 11 2024.0 12 2024.0 13 2024.0 14 3510.0 15 4996.0 16 4996.0 17 4996.0 18 8807.0 19 12618.0 20 12618.0 21 12618.0 22 20363.5 23 28109.0 24 28109.0 25 28109.0 26 40305.5 27 52502.0 28 52502.0 29 52502.0 30 68303.0 31 84104.0 32 84104.0 33 84104.0 34 95782.5 35 107461.0 36 107461.0 37 107461.0 38 114828.5 39 122196.0 40 122196.0 41 122196.0 42 126289.5 43 130383.0 44 130383.0 45 130383.0 46 131305.5 47 132228.0 48 132228.0 49 132228.0 50 123806.0 51 115384.0 52 115384.0 53 115384.0 54 99027.5 55 82671.0 56 82671.0 57 82671.0 58 69362.5 59 56054.0 60 56054.0 61 56054.0 62 44163.5 63 32273.0 64 32273.0 65 32273.0 66 24541.5 67 16810.0 68 16810.0 69 16810.0 70 12070.5 71 7331.0 72 7331.0 73 7331.0 74 5008.5 75 2686.0 76 2686.0 77 2686.0 78 1807.0 79 928.0 80 928.0 81 928.0 82 597.0 83 266.0 84 266.0 85 266.0 86 177.5 87 89.0 88 89.0 89 89.0 90 56.5 91 24.0 92 24.0 93 24.0 94 15.0 95 6.0 96 6.0 97 6.0 98 5.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23827339404638778 2 0.15549202045968838 3 0.15871465300807572 4 0.07210640327016637 5 0.023666207777219412 6 0.02286054964012258 7 0.3176307205504257 8 0.3142066734677641 9 0.019235088023186842 10 0.0361539089022203 11 0.01611316274193662 12 0.018026600817541594 13 0.016515991810485037 14 0.018530137153227114 15 0.015710333673388206 16 0.020040746160283673 17 0.018832258954638425 18 0.015609626406251102 19 0.02467328044859045 20 0.01732164994758187 21 0.015106090070565582 22 0.017019528146170554 23 0.01832872261895291 24 0.01591174820766241 25 0.015508919139114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 992977.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.90188695938916 #Duplication Level Percentage of deduplicated Percentage of total 1 92.97033192895316 78.9335661200887 2 5.4258898737082095 9.213365774233376 3 0.8542613611020649 2.175852045421843 4 0.2504307060216971 0.8504815797525659 5 0.12782673901558633 0.5426365673144327 6 0.0795241829957381 0.40510519171451603 7 0.05279684492421735 0.31377862316978106 8 0.03491875187170649 0.23717343393396664 9 0.025947686552608925 0.19827067954925384 >10 0.1492457870832711 2.3100037288229442 >50 0.015071495113754088 0.9048360133974016 >100 0.011264580122850427 1.9423648671473317 >500 0.0016600416901122505 0.9043357541952731 >1k 8.300208450561252E-4 1.0682296212586044 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2247 0.2262892292570724 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1651 0.1662676980433585 No Hit CTGTAGGACGTGGAATATGGCAAGA 1615 0.16264223642642278 No Hit GTCCTACAGTGTGCATTTCTCATTT 1568 0.15790899487097887 No Hit CTTTAGGACGTGAAATATGGCGAGG 1266 0.1274954001955735 No Hit GTATCAACGCAGAGTACTTTTTTTT 1244 0.1252798403185572 No Hit GTACATGGGAAGCAGTGGTATCAAC 1020 0.10272141247984595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0141453427420777E-4 0.0 10 0.0 0.0 0.0 2.0141453427420777E-4 0.0 11 0.0 0.0 0.0 3.0212180141131164E-4 0.0 12 0.0 0.0 0.0 4.0282906854841553E-4 0.0 13 0.0 0.0 0.0 4.0282906854841553E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 25 0.005967378 19.03468 5 GCACCGT 40 0.0052138474 14.27601 6 GGCGAGG 350 0.0 14.085219 19 ATCCCGT 85 2.759116E-7 13.386168 10 TCCAACG 115 3.8926373E-10 13.190172 18 GTTCTAA 110 2.5484042E-9 13.017676 1 CCCGTTT 90 5.535003E-7 12.640581 12 CCAACGA 85 4.0310915E-6 12.268799 19 TAGGACC 530 0.0 12.22143 4 GCCTTAC 55 0.0029574048 12.149831 1 GTCTTAG 135 3.4197E-10 12.02126 1 ACGAAAT 65 8.1588095E-4 11.668229 12 CGTTTCC 90 7.62584E-6 11.5872 14 GCTAGAC 75 1.9930172E-4 11.448008 3 GTCCTAC 1035 0.0 11.437108 1 CGAAATC 75 2.112433E-4 11.376524 13 TCCCGTT 100 1.975457E-6 11.376523 11 TAGATTG 85 5.2202216E-5 11.196871 5 TGGCGAG 1020 0.0 11.153455 18 CCCTAAT 60 0.005664333 11.137345 1 >>END_MODULE