FastQCFastQC Report
Thu 26 May 2016
SRR1926816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992977
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT21940.2209517440988059No Hit
GTATCAACGCAGAGTACTTTTTTTT21000.21148526098791814No Hit
CTGTAGGACGTGGAATATGGCAAGA19120.1925522947661426No Hit
GTCCTAAAGTGTGTATTTCTCATTT17160.17281367040727025No Hit
GTCCTACAGTGTGCATTTCTCATTT16440.16556274717339878No Hit
CTTTAGGACGTGAAATATGGCGAGG16320.16435425996775352No Hit
GGTATCAACGCAGAGTACTTTTTTT12890.1298116673397269No Hit
GTACATGGGAAGCAGTGGTATCAAC12120.12205720777016991No Hit
CTGTAGGACCTGGAATATGGCGAGA11760.11843174615323417No Hit
TATCAACGCAGAGTACTTTTTTTTT11170.11249001739214505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGA456.826219E-414.75858210
TCCAACG1550.014.69057218
CCAACGA1301.8189894E-1213.86658119
CTAGTAC852.613724E-713.4458123
AAGACGT500.001471630613.3324165
TCCCGTT1052.0265361E-812.65021411
TAGGACC5150.012.3893464
CCGTTTC1001.4668876E-712.33271613
CCGGTTT854.0014147E-612.27691312
AGGACCT7450.012.2714465
TAGGTGC701.06563486E-412.2440546
ATCCCGT1103.8766302E-812.07520410
CGTTTCC951.056218E-611.98320714
GGACCTG7600.011.9039426
ACTGTTC4050.011.7125718
GTCTTAG1251.7305865E-811.4422451
CTAGGCT1354.5401976E-911.28674
AGGACTT1451.1659722E-911.1650775
GTATAGG1201.2094642E-711.1244051
TGTAGGA12350.011.1168452
Produced by FastQC (version 0.11.5)