FastQCFastQC Report
Thu 26 May 2016
SRR1926815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382861
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8280.2162664779123494No Hit
GTACATGGGAAGCAGTGGTATCAAC7590.19824427141965362No Hit
CCCATGTACTCTGCGTTGATACCAC6810.17787134234095403No Hit
TATCAACGCAGAGTACTTTTTTTTT5060.1321628476131024No Hit
GGTATCAACGCAGAGTACTTTTTTT5040.1316404648162127No Hit
GTCCTACAGTGGACATTTCTAAATT4520.11805851209707961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCC307.5882266E-419.044344
GATTTAA400.0051033314.3189441
GTGTAGG400.0051033314.3189441
TATAGTG707.0850074E-613.5977495
GTCCTAA1450.013.1668441
AGGACGT1900.013.025215
ATATACT604.0000115E-412.6962274
GTACAGT604.0177628E-412.68956856
GACGTGA1051.9264917E-812.6895687
GGACGTG2000.012.3723296
GTACAAA550.0029524312.1494061
TCCTATA550.002969934812.1398282
TCAGAAC550.002972860812.1382333
TAGGACG2000.011.9027134
TTAGGAC1458.913048E-1111.8392613
CGTGGAA1052.7649185E-711.740079
AACTGTG657.8787794E-411.7134487
TTCTATA600.005688272411.1281762
TCTACTC600.005693842711.1267143
TCCTAAA1657.2759576E-1110.9836542
Produced by FastQC (version 0.11.5)