##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926815_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 382861 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.265023076260054 31.0 28.0 33.0 16.0 34.0 2 28.335017147215307 31.0 29.0 33.0 16.0 34.0 3 28.13542512817968 31.0 28.0 33.0 16.0 34.0 4 31.68165731166141 35.0 32.0 37.0 18.0 37.0 5 31.902199492766304 35.0 33.0 37.0 19.0 37.0 6 31.08783344346904 35.0 32.0 37.0 11.0 37.0 7 31.134560584650828 35.0 32.0 37.0 11.0 37.0 8 30.93930956665735 35.0 32.0 37.0 11.0 37.0 9 33.07324851577988 37.0 33.0 39.0 17.0 39.0 10 33.25511086268907 38.0 34.0 39.0 17.0 39.0 11 33.40181945928157 38.0 34.0 39.0 17.0 39.0 12 33.263150333933204 38.0 34.0 39.0 17.0 39.0 13 33.38210212061296 38.0 34.0 39.0 17.0 39.0 14 34.42396065412774 39.0 34.0 41.0 17.0 41.0 15 33.99217992953056 39.0 34.0 41.0 10.0 41.0 16 34.0729925482094 39.0 34.0 41.0 11.0 41.0 17 34.30123987556842 39.0 34.0 41.0 16.0 41.0 18 34.36341387605423 39.0 34.0 41.0 15.0 41.0 19 33.77228027926584 39.0 34.0 41.0 10.0 41.0 20 34.01433940777462 39.0 34.0 41.0 10.0 41.0 21 33.98136921754893 39.0 34.0 41.0 8.0 41.0 22 34.019628533593135 39.0 34.0 41.0 2.0 41.0 23 34.02621316874793 39.0 34.0 41.0 2.0 41.0 24 32.77733694473974 39.0 32.0 41.0 2.0 41.0 25 33.33182277641233 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.35046427448227746 1101 2 -0.20318170525807133 1101 3 -1.013771667433236 1101 4 -0.6371350282522172 1101 5 -0.4678358150504174 1101 6 -1.933523962801857 1101 7 -0.6791140166929637 1101 8 -0.22422579361002803 1101 9 -0.7571399257212974 1101 10 -9.12076410546183 1101 11 -3.635977531628299 1101 12 -1.602599281084462 1101 13 -1.2557349845643273 1101 14 -0.8498768054727748 1101 15 -2.519222010474472 1101 16 -0.718738737267806 1101 17 -0.3476709265396778 1101 18 -0.23837021274035664 1101 19 -1.5182317412700002 1101 20 -0.4781322892596407 1101 21 -0.47649513301573876 1101 22 -0.18672118087219047 1101 23 -0.2525230857593783 1101 24 -0.1450983718089205 1101 25 -0.12525924862650584 1102 1 -0.18194564100616617 1102 2 -0.3850572613654215 1102 3 -0.6589079266672044 1102 4 -0.7420970577595334 1102 5 -0.19043394859895102 1102 6 -0.7971095360838731 1102 7 -0.3364898107625294 1102 8 0.2208670116541107 1102 9 0.2615556274891233 1102 10 0.18290672037051792 1102 11 0.07228079187616032 1102 12 -0.04454684662753294 1102 13 -0.06252001156786235 1102 14 -0.4604154277750041 1102 15 0.2303589691021628 1102 16 0.16562197206651774 1102 17 -0.05690179127367401 1102 18 -0.27786159436967495 1102 19 0.4795024445888387 1102 20 0.1406729334936898 1102 21 0.08222980335523289 1102 22 0.14959185324951818 1102 23 -0.25477855371725155 1102 24 -3.184289714122741 1102 25 -1.229176313621224 1103 1 -0.007078484879123437 1103 2 -0.17984897028522795 1103 3 -0.0022332826076585377 1103 4 0.020014382362695926 1103 5 -0.3556133047286991 1103 6 0.3335642155893517 1103 7 0.16413215546199922 1103 8 0.30435921679479705 1103 9 0.10089904749209921 1103 10 0.32071956262095824 1103 11 0.0448669544797653 1103 12 -0.2187244315056418 1103 13 0.028672405852596228 1103 14 -0.18034133266825592 1103 15 0.20419582465726904 1103 16 0.010556327587821102 1103 17 -0.21429304859191944 1103 18 -0.11583263237721297 1103 19 -1.9120726172697609 1103 20 -0.45905123800673664 1103 21 -0.14315182700753581 1103 22 0.09436530881274052 1103 23 0.059199461128812914 1103 24 1.5314379187549392 1103 25 0.5753843213251599 1104 1 -0.22030010434410485 1104 2 -0.31148278560985077 1104 3 -1.4611771489628644 1104 4 -0.6120023232365099 1104 5 -0.2757951615213017 1104 6 -1.9008868999405522 1104 7 -0.367033182350184 1104 8 -0.09652368313248516 1104 9 0.16092272877294533 1104 10 0.1317841262869237 1104 11 0.035288262484421296 1104 12 0.11219572782819398 1104 13 -0.2955809581119553 1104 14 -0.3720599630150474 1104 15 0.15358995710512602 1104 16 0.08786155227040382 1104 17 -0.01708525178760567 1104 18 -0.2576629122410523 1104 19 -1.214452972102869 1104 20 -0.28897716965278164 1104 21 -0.11770410279149246 1104 22 0.06250819445403266 1104 23 0.18492582650266343 1104 24 1.5427026149845915 1104 25 0.9192744865149649 1105 1 -0.1598645467218489 1105 2 -0.4138310767049447 1105 3 0.031849278999622754 1105 4 -0.17769712289550554 1105 5 -0.17416298295817612 1105 6 -0.5057074843007783 1105 7 -0.21491352025610055 1105 8 -0.21161629725323294 1105 9 -0.6921098992980461 1105 10 -0.2514166797271855 1105 11 -0.43186511269964 1105 12 -0.34752681731634993 1105 13 -0.3412726577167007 1105 14 -0.7154064715789517 1105 15 0.03346595493508886 1105 16 -0.6988268147760195 1105 17 -0.8353352711629469 1105 18 -0.6144419558743124 1105 19 -1.3101702471551775 1105 20 -0.5421213996768017 1105 21 -0.3956239663891026 1105 22 -0.18496603634500985 1105 23 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1108 21 0.3758087252252835 1108 22 0.7144268542743575 1108 23 1.075331197153858 1108 24 0.1951799786120887 1108 25 0.6574020306126585 1109 1 0.15187458371293516 1109 2 0.34587906822259384 1109 3 -1.3273536195511006 1109 4 0.028389833626235372 1109 5 0.25243121994037665 1109 6 0.6158867185977748 1109 7 0.7724053203236103 1109 8 1.0436010940511125 1109 9 0.528035028238186 1109 10 0.675322450707597 1109 11 0.3729174959960133 1109 12 -0.020932614417795037 1109 13 0.3085099509789515 1109 14 0.5876370066819234 1109 15 -0.9460267986881021 1109 16 0.2869702866732595 1109 17 0.3200270476776339 1109 18 0.4136739861546701 1109 19 -1.5804601022276472 1109 20 -0.7050199504014429 1109 21 0.0424919756398765 1109 22 0.16481657235064517 1109 23 0.31574579085203425 1109 24 -0.8203009500777974 1109 25 -0.2078467256062453 1110 1 0.37588109837091466 1110 2 0.49237201067644776 1110 3 0.8984064238799512 1110 4 0.7560119834633703 1110 5 0.9837721537080171 1110 6 0.8080582712579272 1110 7 0.8563086862302356 1110 8 1.202982201542973 1110 9 0.9504563094542533 1110 10 0.9115602357238899 1110 11 0.47334094876474353 1110 12 0.4766623910110823 1110 13 0.6053666285033827 1110 14 0.07157837476010087 1110 15 0.8004281415942032 1110 16 0.6743752812443802 1110 17 0.2040444200989171 1110 18 0.3052917928864858 1110 19 -1.404423185832421 1110 20 0.14557722658010164 1110 21 0.472383398006869 1110 22 0.24343220752979988 1110 23 0.45983916761642263 1110 24 -0.1467450325967974 1110 25 0.13874936559896867 1111 1 0.5171080780768627 1111 2 0.09856827917267452 1111 3 1.0082743997725707 1111 4 0.7274880622896838 1111 5 0.9071252618083321 1111 6 1.3316516139278889 1111 7 1.0820268984234467 1111 8 1.4361775835841257 1111 9 0.7873748027953482 1111 10 0.6762483766335237 1111 11 0.752933599055595 1111 12 0.6840351794630379 1111 13 0.3413682440546353 1111 14 0.6862038343791852 1111 15 -0.384054173889389 1111 16 0.10387849923364456 1111 17 0.21484186249244885 1111 18 0.5041409748825316 1111 19 1.0381067254696177 1111 20 0.7207449449286685 1111 21 0.6929428613081541 1111 22 0.7556458799190864 1111 23 0.8603673689518772 1111 24 1.2994172463795337 1111 25 1.1515977188381967 1112 1 -0.4945356726973209 1112 2 -0.21905682921330438 1112 3 -2.0433792605767422 1112 4 -0.9393024740660714 1112 5 -0.7485944210852651 1112 6 0.38761748782854255 1112 7 0.24612326904156134 1112 8 0.7707805812305999 1112 9 -0.2889521512489921 1112 10 0.006732707117855341 1112 11 0.08054570112421544 1112 12 -0.08817620416138539 1112 13 -0.3448233823543845 1112 14 -0.11069632665141427 1112 15 0.13224243208112796 1112 16 -0.02200407230110102 1112 17 -0.7217678241172365 1112 18 -0.3460696035889157 1112 19 0.24120656443902533 1112 20 0.21901851113702264 1112 21 0.1465304371783418 1112 22 0.1653293928634625 1112 23 0.006835534441783864 1112 24 1.4035452037683598 1112 25 0.42029430003478296 1113 1 -0.3502897132854841 1113 2 0.2047416274642977 1113 3 -4.716729606912871 1113 4 -0.8859545581589146 1113 5 0.2662463858171549 1113 6 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-2.127769118788489 2216 14 -2.4939327607599395 2216 15 -3.4914531552772488 2216 16 -2.252661496272477 2216 17 -2.7114070608494956 2216 18 -2.489368649504243 2216 19 -1.781080257266403 2216 20 -2.6882563845099696 2216 21 -5.6570622879260135 2216 22 -4.078148543928425 2216 23 -3.9785177750394354 2216 24 -4.4528203299227584 2216 25 -3.6213350976992693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23853.0 3 2.0 4 184.0 5 1109.0 6 1533.0 7 1187.0 8 985.0 9 847.0 10 746.0 11 718.0 12 704.0 13 697.0 14 671.0 15 681.0 16 736.0 17 784.0 18 955.0 19 1213.0 20 1475.0 21 1909.0 22 2323.0 23 2702.0 24 3303.0 25 3617.0 26 4213.0 27 4414.0 28 5160.0 29 5930.0 30 7290.0 31 8863.0 32 11275.0 33 15038.0 34 20423.0 35 28801.0 36 42982.0 37 70179.0 38 105359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64632205093834 18.401265080428956 12.258346598525469 25.694066270107243 2 15.212762618230519 20.513256466058426 39.458127563405036 24.815853352306018 3 18.682382237847115 24.469520999434994 29.54203025927554 27.30606650344236 4 12.876786082992908 16.417196578247882 36.690790385012455 34.015226953746755 5 13.213575670175274 37.74717652499 35.36299582258937 13.676251982245352 6 32.309412530010945 36.08367273895767 18.26050154528616 13.346413185745226 7 28.08721464895894 31.48038010998771 21.94435638324351 18.488048857809837 8 25.571007141846607 35.66495674545578 21.10957992528046 17.654456187417146 9 26.385692304075114 15.681305791471498 19.929882676266438 38.003119228186954 10 15.263019003002123 28.806544578539906 33.669515505749494 22.26092091270847 11 33.981624917385275 23.339541955219552 23.66686607401757 19.011967053377603 12 23.2057116285874 25.554469981556853 30.39435942716524 20.845458962690504 13 28.605127454924478 22.407667671537766 25.29819593419052 23.689008939347232 14 21.29521023001868 22.341462140310085 26.657610470356197 29.705717159315036 15 23.75353315813397 30.353550921886512 22.96435232835774 22.928563591621778 16 22.590572327241187 28.255326708256266 26.76442312716177 22.389677837340773 17 21.860754301955897 28.91447291080511 27.156072112458236 22.068700674780757 18 22.04522418783503 27.718362399556955 28.54254396505784 21.69386944755018 19 23.567953936265262 27.34354997988369 27.189412029030187 21.89908405482086 20 23.409780220641444 27.366749130731232 27.534725034156843 21.688745614470484 21 23.00626959247649 27.038401253918494 27.057471264367816 22.8978578892372 22 23.323267101708993 27.45625630227953 26.7924597307196 22.428016865291877 23 22.51091976843821 27.50475454032477 27.228364229137497 22.75596146209952 24 23.40175968400924 27.065068600507843 27.232259480245354 22.30091223523757 25 22.825817204329166 26.998503130885922 27.346988889730646 22.828690775054273 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 478.0 1 478.0 2 522.5 3 567.0 4 567.0 5 567.0 6 664.0 7 761.0 8 761.0 9 761.0 10 908.0 11 1055.0 12 1055.0 13 1055.0 14 1529.0 15 2003.0 16 2003.0 17 2003.0 18 3240.0 19 4477.0 20 4477.0 21 4477.0 22 6804.5 23 9132.0 24 9132.0 25 9132.0 26 12707.5 27 16283.0 28 16283.0 29 16283.0 30 20894.0 31 25505.0 32 25505.0 33 25505.0 34 29383.0 35 33261.0 36 33261.0 37 33261.0 38 36831.5 39 40402.0 40 40402.0 41 40402.0 42 42727.0 43 45052.0 44 45052.0 45 45052.0 46 47570.5 47 50089.0 48 50089.0 49 50089.0 50 48692.5 51 47296.0 52 47296.0 53 47296.0 54 42930.0 55 38564.0 56 38564.0 57 38564.0 58 33552.5 59 28541.0 60 28541.0 61 28541.0 62 23740.0 63 18939.0 64 18939.0 65 18939.0 66 15002.0 67 11065.0 68 11065.0 69 11065.0 70 8289.0 71 5513.0 72 5513.0 73 5513.0 74 3953.5 75 2394.0 76 2394.0 77 2394.0 78 1694.0 79 994.0 80 994.0 81 994.0 82 675.5 83 357.0 84 357.0 85 357.0 86 229.0 87 101.0 88 101.0 89 101.0 90 64.5 91 28.0 92 28.0 93 28.0 94 14.5 95 1.0 96 1.0 97 1.0 98 2.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23742298118638355 2 0.1454836089337906 3 0.14757314012134953 4 0.0652978496112166 5 0.02298484306314824 6 0.02194007746936878 7 0.3087282329618321 8 0.3048103619851591 9 0.019328163484920117 10 0.03447726459472237 11 0.015671483906691985 12 0.016455058102026587 13 0.015410292508247119 14 0.017238632297361185 15 0.015410292508247119 16 0.01906697208647525 17 0.01828339789114065 18 0.014887909711357386 19 0.022723651664703377 20 0.017761015094250918 21 0.015932675305136854 22 0.016455058102026587 23 0.018022206492695782 24 0.016977440898916316 25 0.01619386670358172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 382861.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.37430676186808 #Duplication Level Percentage of deduplicated Percentage of total 1 90.28378095788231 75.27347649203784 2 7.182737320181065 11.97711489445389 3 1.4200103826660169 3.551771437483025 4 0.45186679611846525 1.506963235003337 5 0.21122195123693008 0.8805241878634077 6 0.1158050051373856 0.5793097213730457 7 0.07539259665866911 0.4400063836975569 8 0.04770360714291035 0.318180414046452 9 0.037532473034802144 0.2816319528301622 >10 0.14947728941147911 2.0476446266729416 >50 0.015059458807575456 0.8637288619093735 >100 0.007843468102006222 1.4221967927164445 >500 0.0015686936204012444 0.8574509999125447 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 828 0.2162664779123494 No Hit GTACATGGGAAGCAGTGGTATCAAC 759 0.19824427141965362 No Hit CCCATGTACTCTGCGTTGATACCAC 681 0.17787134234095403 No Hit TATCAACGCAGAGTACTTTTTTTTT 506 0.1321628476131024 No Hit GGTATCAACGCAGAGTACTTTTTTT 504 0.1316404648162127 No Hit GTCCTACAGTGGACATTTCTAAATT 452 0.11805851209707961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACCCC 30 7.5882266E-4 19.04434 4 GATTTAA 40 0.00510333 14.318944 1 GTGTAGG 40 0.00510333 14.318944 1 TATAGTG 70 7.0850074E-6 13.597749 5 GTCCTAA 145 0.0 13.166844 1 AGGACGT 190 0.0 13.02521 5 ATATACT 60 4.0000115E-4 12.696227 4 GTACAGT 60 4.0177628E-4 12.6895685 6 GACGTGA 105 1.9264917E-8 12.689568 7 GGACGTG 200 0.0 12.372329 6 GTACAAA 55 0.00295243 12.149406 1 TCCTATA 55 0.0029699348 12.139828 2 TCAGAAC 55 0.0029728608 12.138233 3 TAGGACG 200 0.0 11.902713 4 TTAGGAC 145 8.913048E-11 11.839261 3 CGTGGAA 105 2.7649185E-7 11.74007 9 AACTGTG 65 7.8787794E-4 11.713448 7 TTCTATA 60 0.0056882724 11.128176 2 TCTACTC 60 0.0056938427 11.126714 3 TCCTAAA 165 7.2759576E-11 10.983654 2 >>END_MODULE