FastQCFastQC Report
Thu 26 May 2016
SRR1926815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382861
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16380.4278315106526912No Hit
GGTATCAACGCAGAGTACTTTTTTT10880.2841762415080147No Hit
TATCAACGCAGAGTACTTTTTTTTT9230.24107966076461168No Hit
GTACATGGGAAGCAGTGGTATCAAC8400.21940077469368777No Hit
CCCATGTACTCTGCGTTGATACCAC8170.21339337252945587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5680.14835671431668412No Hit
GTCCTACAGTGGACATTTCTAAATT3950.10317060238572223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAAG350.002138539516.3170413
TGTACTG456.654844E-414.8042635
GACTGTC400.005298423614.2382437
GTATATA551.9011961E-413.8629221
TGGAGTG551.9246049E-413.8429495
TAGGACC1207.1122486E-1012.6893694
GTCCTAG550.002987922412.1300561
CCAGGAC1052.6325324E-711.7845293
ATATCTG752.0319353E-411.4204325
GATATAC600.005722512511.1192181
CTACAAC600.00577865211.1046533
AGGACCT1900.011.0197165
GAAGAGT700.001464893510.8766026
GTAGGAC2450.010.4895263
TAGACAG750.002600830310.1514955
AGACAGC856.461447E-410.0768526
GGACCTG1701.4897523E-910.0768526
TGTAGGA2900.09.8581122
GTGCATT1356.481059E-79.84115211
GTGTAGA800.00438378439.5307581
Produced by FastQC (version 0.11.5)