##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926815_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 382861 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.651816194389088 31.0 30.0 33.0 25.0 34.0 2 29.90514311982678 31.0 30.0 34.0 26.0 34.0 3 29.985052016267 31.0 30.0 34.0 26.0 34.0 4 33.5787034981364 35.0 35.0 37.0 28.0 37.0 5 33.521100347123365 37.0 35.0 37.0 28.0 37.0 6 33.42415393576259 37.0 35.0 37.0 28.0 37.0 7 33.38140473957912 37.0 35.0 37.0 28.0 37.0 8 33.40276236022995 37.0 35.0 37.0 28.0 37.0 9 34.9193649914721 39.0 35.0 39.0 28.0 39.0 10 34.80040536905039 39.0 35.0 39.0 27.0 39.0 11 34.83986094169947 39.0 35.0 39.0 27.0 39.0 12 34.735491470794884 39.0 35.0 39.0 27.0 39.0 13 34.738615319920285 39.0 35.0 39.0 27.0 39.0 14 35.815058206503146 40.0 36.0 41.0 27.0 41.0 15 35.78403650410985 40.0 36.0 41.0 27.0 41.0 16 35.74606188668995 40.0 36.0 41.0 27.0 41.0 17 35.73141949689313 40.0 36.0 41.0 27.0 41.0 18 35.70143211243767 40.0 36.0 41.0 27.0 41.0 19 35.767850473148215 40.0 36.0 41.0 27.0 41.0 20 35.723884125047995 40.0 36.0 41.0 27.0 41.0 21 35.67758011393169 40.0 36.0 41.0 27.0 41.0 22 35.57958632506314 40.0 36.0 41.0 27.0 41.0 23 35.49631850723892 40.0 36.0 41.0 27.0 41.0 24 35.4072548522832 40.0 36.0 41.0 26.0 41.0 25 35.29293137718388 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31099804229496897 1101 2 -0.017601414821573513 1101 3 -0.1356244165502396 1101 4 -0.09671542813165246 1101 5 -0.11395702225532034 1101 6 -0.36612031514051324 1101 7 -0.17168946191883805 1101 8 -0.08487009416980129 1101 9 -0.10327171853951711 1101 10 -0.1483923790495112 1101 11 -0.22076416215134742 1101 12 -0.24177076649854712 1101 13 -0.16288647029666947 1101 14 -0.11404424617823139 1101 15 -0.6066852859177132 1101 16 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2211 13 -0.06629166294462863 2211 14 0.14067716356430537 2211 15 0.12180205745425354 2211 16 0.361059063575766 2211 17 0.21433325504675338 2211 18 0.19605507715596815 2211 19 -2.3016883234561192E-4 2211 20 0.024795105006155893 2211 21 -0.015857364326052448 2211 22 0.15061206619753165 2211 23 0.3817332040436199 2211 24 0.40700296264434854 2211 25 0.6080023780872352 2212 1 0.48586975254559306 2212 2 0.3882105658631616 2212 3 0.609544592019823 2212 4 0.6786764600370248 2212 5 0.2779514477494658 2212 6 0.37518854058953366 2212 7 0.2361961545890452 2212 8 0.17838014872110364 2212 9 0.3513011559837693 2212 10 0.3254174554946516 2212 11 0.3557791014324181 2212 12 0.1429597333491941 2212 13 0.34444375983340336 2212 14 0.5156449984672946 2212 15 0.4790559301319348 2212 16 0.42469191829246 2212 17 0.5192995460250884 2212 18 0.7831710258976727 2212 19 0.6452821568328346 2212 20 0.642671179320395 2212 21 0.49310769151255585 2212 22 0.5535686819146832 2212 23 0.6004043995559485 2212 24 0.35162238521652256 2212 25 0.5328132473168168 2213 1 -0.09335118595858916 2213 2 0.02691589124889049 2213 3 0.14324341704987376 2213 4 -0.22339138895848976 2213 5 -0.18423119998031723 2213 6 -0.1554440789311542 2213 7 -0.15379820359281382 2213 8 -0.11630673037227979 2213 9 -0.2588026044106684 2213 10 -0.13535473770082262 2213 11 -0.18193522633269055 2213 12 -0.1638030895222471 2213 13 -0.38530088657177686 2213 14 -0.3161061728232113 2213 15 -0.5577532537668048 2213 16 -0.38720005315503414 2213 17 -0.3570735008020023 2213 18 -0.1922993300274669 2213 19 -0.3963421961978071 2213 20 -0.38441935959588136 2213 21 -0.3761591174260488 2213 22 -0.9055085128752225 2213 23 -0.4909685246229216 2213 24 -0.6864549812808889 2213 25 -0.5400643252296007 2214 1 0.2656626184682658 2214 2 -0.07445540305455722 2214 3 0.19148668697047455 2214 4 -0.14458048601319007 2214 5 0.23342614015170682 2214 6 0.3168989179824706 2214 7 0.14268400280430882 2214 8 0.07145417907318574 2214 9 -0.013291330233663246 2214 10 -0.024502830744680182 2214 11 0.06817672097263028 2214 12 0.006660155649569788 2214 13 -0.058725318529482706 2214 14 0.19354055518576274 2214 15 0.10688150912157823 2214 16 0.3503232391068778 2214 17 0.2012209519080841 2214 18 0.14240626059689276 2214 19 -0.009536570424408808 2214 20 -0.08236867755993416 2214 21 0.009594444424237736 2214 22 0.17451575441160827 2214 23 0.19988838986859747 2214 24 -0.08166267362561541 2214 25 0.16508332483997634 2215 1 0.3435740290319913 2215 2 0.14090753413135104 2215 3 -0.035035539056607945 2215 4 0.13970951204324678 2215 5 0.14026933166666566 2215 6 0.41005109893740865 2215 7 0.1888436155725941 2215 8 0.1143860895952642 2215 9 -0.051320372612586596 2215 10 0.13253471703456654 2215 11 -0.528527089335654 2215 12 -0.13999090081554755 2215 13 0.31100276780171754 2215 14 0.09146186866391304 2215 15 0.09927847716385685 2215 16 0.2303498936802626 2215 17 -0.008681499757358324 2215 18 0.16441247999734543 2215 19 -0.11495170614986705 2215 20 0.16171733896960205 2215 21 0.08286675606062488 2215 22 0.0020268836215038277 2215 23 0.10920962375322318 2215 24 0.12905885086789937 2215 25 0.09676015664776116 2216 1 -0.49743000940276616 2216 2 -0.42541331254738424 2216 3 -2.0969262924335723 2216 4 -1.5656675245811797 2216 5 -0.71088301480318 2216 6 -0.3950160922615922 2216 7 -0.5546983702232566 2216 8 -0.6845799318502159 2216 9 -0.6207483770687858 2216 10 -0.7169900705994721 2216 11 -0.5645422682800927 2216 12 -0.5372545131258164 2216 13 -0.6639223818988782 2216 14 -0.8085172428804412 2216 15 -0.6769800522888687 2216 16 -0.6092441739983272 2216 17 -0.559701552674781 2216 18 -0.380523810212928 2216 19 -0.5474732908873676 2216 20 -0.60749133670231 2216 21 -0.7119737953946057 2216 22 -0.516938093401194 2216 23 -0.495537277792522 2216 24 -0.5862140916192189 2216 25 -0.5032607318078846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11354.0 3 8.0 4 210.0 5 1394.0 6 1817.0 7 1300.0 8 988.0 9 835.0 10 738.0 11 670.0 12 595.0 13 582.0 14 565.0 15 540.0 16 586.0 17 622.0 18 650.0 19 778.0 20 1007.0 21 1264.0 22 1644.0 23 1949.0 24 2284.0 25 2672.0 26 3054.0 27 3403.0 28 3927.0 29 4839.0 30 6086.0 31 7854.0 32 9829.0 33 13228.0 34 17855.0 35 25029.0 36 36585.0 37 63520.0 38 152600.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45580271576982 19.14479137522128 10.316316845042138 28.08308906396676 2 15.206753791554098 20.86289729916336 37.859573232765726 26.070775676516817 3 18.32644968141884 24.496657444924253 29.382613488822884 27.79427938483402 4 12.717670381678992 17.152177944475934 36.07523357040806 34.05491810343702 5 13.633442866173159 38.23549386424545 34.12487788568533 14.00618538389606 6 32.71265861929796 36.587660584840684 17.067986812518672 13.63169398334268 7 28.62924084166305 32.16168625217496 20.784238934492613 18.424833971669376 8 26.20409698603514 36.12708911284213 19.48576085763992 18.183053043482804 9 26.73568118531521 15.45068413710954 19.51031560689414 38.303319070681106 10 15.484154078826046 29.09913463221216 32.81527937039465 22.60143191856714 11 34.047560586984574 23.273029323107945 23.371243189025122 19.308166900882355 12 23.436104247527805 25.21352564136051 30.265006869387605 21.085363241724085 13 28.90616837662202 21.99025220970809 25.269291848632385 23.834287565037506 14 21.575454277139745 22.125000979467742 26.57544121756982 29.724103525822688 15 23.765766659962544 30.01548913540325 23.02970622882651 23.189037975807693 16 22.61894811626303 28.137994441774456 26.62175857241365 22.621298869548863 17 22.001776106148462 28.627696808232773 27.081439690748578 22.289087394870187 18 22.2704560710657 27.344935576468497 28.658219648589423 21.72638870387638 19 23.44297277602054 26.99491460347228 27.541065817620492 22.021046802886687 20 23.5414266945622 26.932771243571356 27.792883504807275 21.732918557059165 21 23.06689619322026 26.406089593722328 27.44052641340379 23.08648779965362 22 23.5952473613139 27.08450325313887 26.757752813684338 22.562496571862894 23 22.53695867941284 27.04748472026328 27.679308363370424 22.736248236953454 24 23.092715110705974 26.774730254583258 27.66095266950669 22.471601965204083 25 22.898360689993837 26.750321279685718 27.553259290990585 22.798058739329857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 256.0 2 249.5 3 243.0 4 243.0 5 243.0 6 374.0 7 505.0 8 505.0 9 505.0 10 721.0 11 937.0 12 937.0 13 937.0 14 1313.5 15 1690.0 16 1690.0 17 1690.0 18 2864.0 19 4038.0 20 4038.0 21 4038.0 22 6340.0 23 8642.0 24 8642.0 25 8642.0 26 12160.5 27 15679.0 28 15679.0 29 15679.0 30 20385.0 31 25091.0 32 25091.0 33 25091.0 34 29062.5 35 33034.0 36 33034.0 37 33034.0 38 36146.0 39 39258.0 40 39258.0 41 39258.0 42 41773.0 43 44288.0 44 44288.0 45 44288.0 46 47610.5 47 50933.0 48 50933.0 49 50933.0 50 49707.5 51 48482.0 52 48482.0 53 48482.0 54 43754.0 55 39026.0 56 39026.0 57 39026.0 58 34242.5 59 29459.0 60 29459.0 61 29459.0 62 24716.0 63 19973.0 64 19973.0 65 19973.0 66 15739.0 67 11505.0 68 11505.0 69 11505.0 70 8651.5 71 5798.0 72 5798.0 73 5798.0 74 4150.5 75 2503.0 76 2503.0 77 2503.0 78 1762.5 79 1022.0 80 1022.0 81 1022.0 82 699.0 83 376.0 84 376.0 85 376.0 86 240.5 87 105.0 88 105.0 89 105.0 90 60.0 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11178991853440282 2 0.13007331642554346 3 0.01828339789114065 4 0.0 5 0.006007402164231927 6 0.33615332979854307 7 0.02481318285226231 8 0.011231230133129256 9 0.011231230133129256 10 0.004701445172007596 11 0.006007402164231927 12 7.835741953345993E-4 13 0.0013059569922243323 14 0.0 15 0.003134296781338397 16 0.0013059569922243323 17 2.6119139844486643E-4 18 0.0010447655937794657 19 0.0 20 0.0010447655937794657 21 0.01149242153157412 22 0.0 23 2.6119139844486643E-4 24 0.0 25 0.004440253773562729 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 382861.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.0184460037548 #Duplication Level Percentage of deduplicated Percentage of total 1 88.1699665201278 70.55223705143716 2 8.673200162616126 13.880319977841118 3 1.775124040685024 4.2612800159856485 4 0.5690440263967834 1.8213607479996095 5 0.2611827011237032 1.0449716933490938 6 0.13523157938883376 0.6492612499996725 7 0.08908083102760474 0.4989676767286396 8 0.06368685298803237 0.40768984055775503 9 0.05087772315783815 0.3664040708969512 >10 0.18613916997135346 2.507284186886677 >50 0.014376805820778632 0.8507802347991409 >100 0.010129113177850509 1.623646359181594 >500 0.0013069823455290979 0.8230658194369819 >1k 6.534911727645489E-4 0.712731074900004 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1638 0.4278315106526912 No Hit GGTATCAACGCAGAGTACTTTTTTT 1088 0.2841762415080147 No Hit TATCAACGCAGAGTACTTTTTTTTT 923 0.24107966076461168 No Hit GTACATGGGAAGCAGTGGTATCAAC 840 0.21940077469368777 No Hit CCCATGTACTCTGCGTTGATACCAC 817 0.21339337252945587 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 568 0.14835671431668412 No Hit GTCCTACAGTGGACATTTCTAAATT 395 0.10317060238572223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.6119139844486643E-4 13 0.0 0.0 0.0 0.0 2.6119139844486643E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAAAG 35 0.0021385395 16.317041 3 TGTACTG 45 6.654844E-4 14.804263 5 GACTGTC 40 0.0052984236 14.238243 7 GTATATA 55 1.9011961E-4 13.862922 1 TGGAGTG 55 1.9246049E-4 13.842949 5 TAGGACC 120 7.1122486E-10 12.689369 4 GTCCTAG 55 0.0029879224 12.130056 1 CCAGGAC 105 2.6325324E-7 11.784529 3 ATATCTG 75 2.0319353E-4 11.420432 5 GATATAC 60 0.0057225125 11.119218 1 CTACAAC 60 0.005778652 11.104653 3 AGGACCT 190 0.0 11.019716 5 GAAGAGT 70 0.0014648935 10.876602 6 GTAGGAC 245 0.0 10.489526 3 TAGACAG 75 0.0026008303 10.151495 5 AGACAGC 85 6.461447E-4 10.076852 6 GGACCTG 170 1.4897523E-9 10.076852 6 TGTAGGA 290 0.0 9.858112 2 GTGCATT 135 6.481059E-7 9.841152 11 GTGTAGA 80 0.0043837843 9.530758 1 >>END_MODULE