FastQCFastQC Report
Thu 26 May 2016
SRR1926814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935181
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT31830.34036191924344056No Hit
CTGTAGGACGTGGAATATGGCAAGA24060.25727639890031984No Hit
GTCCTAAAGTGTGTATTTCTCATTT23750.253961532580324No Hit
GTCCTACAGTGTGCATTTCTCATTT20560.21985048883585104No Hit
CTTTAGGACGTGAAATATGGCGAGG17870.19108600367201645No Hit
CTGTAGGACCTGGAATATGGCGAGA14260.1524838507198072No Hit
GTATCAACGCAGAGTACTTTTTTTT10730.11473714714050007No Hit
CTGAAGGACCTGGAATATGGCGAGA9970.1066103780979297No Hit
GTCCTTCAGTGTGCATTTCTCATTT9510.10169154420374238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5350.015.4828344
TGTCGAG456.851948E-414.7511118
CCTACCG400.00515575314.3000113
TCAATAC957.0440365E-813.0456243
TGTAGGA15350.012.7318222
GACAGTC604.0282076E-412.6892857
TGGCGAA604.1520922E-412.64380918
GTAGGAC14500.012.4262163
GTATTAG1404.9112714E-1112.2696881
GGACCTG9100.012.2360976
CTGTAGG15350.012.1853141
CTACCGT550.003016430212.1177044
GGCGAAA550.003108637212.0690919
AAGACTC951.0154854E-612.0220735
AGGACCT9800.011.9454035
GTATAAG1209.340511E-911.92886451
TTAGTAC802.7626118E-511.9166763
CCTCGAA658.140576E-411.67120817
TAAGACT1001.8760275E-611.4252634
ATGGCAA10900.011.2228317
Produced by FastQC (version 0.11.5)