##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926814_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 935181 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.428350233805006 31.0 29.0 33.0 20.0 34.0 2 28.50483061567761 31.0 30.0 31.0 20.0 34.0 3 28.293599848585462 31.0 28.0 33.0 16.0 34.0 4 31.82361382448959 35.0 32.0 37.0 19.0 37.0 5 32.092412057131185 35.0 33.0 37.0 19.0 37.0 6 31.295937363996917 35.0 32.0 37.0 17.0 37.0 7 31.354440477297977 35.0 32.0 37.0 17.0 37.0 8 31.17677861291023 35.0 32.0 37.0 15.0 37.0 9 33.351527672183245 37.0 33.0 39.0 17.0 39.0 10 33.51350701094227 38.0 34.0 39.0 18.0 39.0 11 33.67803451952082 38.0 34.0 39.0 22.0 39.0 12 33.53803916033367 38.0 34.0 39.0 18.0 39.0 13 33.6547278013561 38.0 34.0 39.0 19.0 39.0 14 34.688938291090174 39.0 35.0 41.0 19.0 41.0 15 34.28636488551414 39.0 34.0 41.0 17.0 41.0 16 34.386976424884594 39.0 34.0 41.0 17.0 41.0 17 34.625491749725455 39.0 34.0 41.0 19.0 41.0 18 34.678213094577416 39.0 35.0 41.0 19.0 41.0 19 34.064340485959406 39.0 34.0 41.0 11.0 41.0 20 34.33986041204858 39.0 34.0 41.0 18.0 41.0 21 34.31415950495145 39.0 34.0 41.0 16.0 41.0 22 34.34797434935055 39.0 34.0 41.0 11.0 41.0 23 34.34690931488129 39.0 34.0 41.0 10.0 41.0 24 33.089775134439215 39.0 32.0 41.0 2.0 41.0 25 33.65890881016616 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.029334919173578555 1101 2 0.025443107213245497 1101 3 -1.0439712588378391 1101 4 -0.19853894060721444 1101 5 0.04780972827852992 1101 6 -2.192633872480016 1101 7 -0.603454095325425 1101 8 0.15470167439111293 1101 9 0.0812939532006638 1101 10 -8.624832318642977 1101 11 -3.658772379758666 1101 12 -1.3890715603809625 1101 13 -0.6428644440951317 1101 14 -0.4431083919773471 1101 15 -1.6610931508378926 1101 16 -0.3268572550143247 1101 17 -0.04512116483510198 1101 18 -0.12538616034957784 1101 19 -0.9173237210832426 1101 20 -0.199800750064135 1101 21 -0.18387327799028697 1101 22 0.06343564890990905 1101 23 0.14346862719898468 1101 24 0.03873516260181731 1101 25 0.1815592213876016 1102 1 -0.2158926872875071 1102 2 -0.4319724581318347 1102 3 -0.5491107595720557 1102 4 -0.6170411432503862 1102 5 -0.16144137304305417 1102 6 -1.0913122865769331 1102 7 -0.3303263420214577 1102 8 0.27070754810623754 1102 9 -0.001672272937369712 1102 10 0.2386038047050434 1102 11 -0.05745079385557972 1102 12 0.12561272743107565 1102 13 0.08033673064789326 1102 14 -0.3146208736219904 1102 15 0.2831065554763512 1102 16 0.07475801664499926 1102 17 -0.021626304335832458 1102 18 -0.23331567576807544 1102 19 0.1693135770077987 1102 20 0.2605516728433557 1102 21 0.17515755901411723 1102 22 -0.106902089709763 1102 23 -0.26255193080395145 1102 24 -3.6675790811573634 1102 25 -1.985841659669667 1103 1 -0.3006468663550059 1103 2 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25 -0.8729757637417634 2215 1 0.008724373587302381 2215 2 -0.5049459019706575 2215 3 -0.04510092580040492 2215 4 0.26092440536400474 2215 5 0.20203208324007704 2215 6 0.7708310110550514 2215 7 0.173962797412905 2215 8 -0.8910615905538144 2215 9 -0.37580220814398757 2215 10 -0.5705695888034512 2215 11 -0.7727691884840624 2215 12 -1.0129284120807185 2215 13 -0.742232273060516 2215 14 -0.574948960986589 2215 15 -0.6629542667884536 2215 16 -0.34101749903344825 2215 17 -0.7698847920477689 2215 18 -1.3957858439875181 2215 19 -0.2947735221125285 2215 20 -4.0459752303758485 2215 21 -2.6230330380639586 2215 22 -1.6891562246780083 2215 23 -1.6775558991566797 2215 24 -1.3689709082865136 2215 25 -1.1539090027538066 2216 1 -0.8875873342136842 2216 2 -1.4893067273267846 2216 3 -0.7412004529863374 2216 4 -0.604686495395633 2216 5 -0.9756100265949144 2216 6 -2.071763430139743 2216 7 -9.667278856148144 2216 8 -11.020069116894447 2216 9 -6.016200432084823 2216 10 -3.64489234852136 2216 11 -2.603026253160621 2216 12 -2.2861518177975313 2216 13 -2.043933493021214 2216 14 -2.186525725263344 2216 15 -3.10878185486073 2216 16 -2.1486243219712193 2216 17 -2.4750389674174613 2216 18 -2.237587137542043 2216 19 -1.6918630049670327 2216 20 -2.667915453196631 2216 21 -5.4674994322272 2216 22 -4.0310713532103435 2216 23 -3.653298735628553 2216 24 -3.8539211458610865 2216 25 -3.27746451472127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51811.0 3 7.0 4 402.0 5 2329.0 6 3082.0 7 2491.0 8 2078.0 9 1827.0 10 1737.0 11 1580.0 12 1583.0 13 1638.0 14 1607.0 15 1638.0 16 1762.0 17 1937.0 18 2203.0 19 2758.0 20 3652.0 21 4629.0 22 5759.0 23 6817.0 24 8169.0 25 9276.0 26 10439.0 27 11673.0 28 12959.0 29 15286.0 30 18569.0 31 22157.0 32 28786.0 33 37851.0 34 51565.0 35 72159.0 36 106062.0 37 172938.0 38 253965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.991198134158786 18.948985191600283 12.21864469272785 25.841171981513078 2 13.523287154879684 20.293069162737552 43.61395569481314 22.569687987569615 3 19.012295705177216 24.409041551816085 29.79090233470619 26.78776040830051 4 11.417974175846519 16.743858042064304 39.67033860760171 32.167829174487466 5 11.894465842156547 38.421544079346354 37.420837081349696 12.263152997147406 6 30.50888609371257 38.276089263100246 19.40672382517028 11.808300818016908 7 27.29559114367715 31.03112840466926 22.915906975147898 18.75737347650569 8 23.324599496562058 39.31974744446792 21.76033822792847 15.595314831041552 9 25.818299272290123 15.520413096210861 20.756095265393782 37.90519236610523 10 13.488779586099481 30.211999392392496 36.106540980344754 20.192680041163268 11 34.24127468291749 22.556895174271048 25.446706930093722 17.755123212717738 12 22.97634632167976 24.942352447935384 33.3407130756199 18.74058815476496 13 29.044836809755587 22.766314479050024 25.939853242682187 22.248995468512202 14 19.16653119197762 23.55730151532005 28.371501544411455 28.90466574829088 15 21.991330694539066 33.62088419215356 23.138254353101477 21.249530760205896 16 20.17953574832053 29.08851328442608 30.37476188999184 20.357189077261552 17 20.195149185487985 29.9426616911432 29.390562548597455 20.47162657477136 18 19.87705181021317 28.614544163383343 32.02749008585839 19.480913940545097 19 23.055892781696734 27.378424776583966 27.73428600461004 21.83139643710926 20 22.451777816993673 28.853630233316757 29.19448767105706 19.500104278632506 21 21.401398682171454 27.870330462459215 27.669265889716083 23.05900496565325 22 21.488243252637716 30.14946603992535 28.233413012903814 20.128877694533127 23 20.782040449630475 29.43945924555343 28.83036182205157 20.948138482764524 24 22.562170325086736 27.717719130877466 29.655555222820666 20.06455532121513 25 21.359489119402618 28.601848316547468 29.25125105212441 20.7874115119255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 263.0 2 345.0 3 427.0 4 427.0 5 427.0 6 665.0 7 903.0 8 903.0 9 903.0 10 1356.0 11 1809.0 12 1809.0 13 1809.0 14 3125.5 15 4442.0 16 4442.0 17 4442.0 18 8132.5 19 11823.0 20 11823.0 21 11823.0 22 18982.0 23 26141.0 24 26141.0 25 26141.0 26 38462.0 27 50783.0 28 50783.0 29 50783.0 30 67993.5 31 85204.0 32 85204.0 33 85204.0 34 95882.0 35 106560.0 36 106560.0 37 106560.0 38 112744.5 39 118929.0 40 118929.0 41 118929.0 42 121787.0 43 124645.0 44 124645.0 45 124645.0 46 124537.0 47 124429.0 48 124429.0 49 124429.0 50 115184.0 51 105939.0 52 105939.0 53 105939.0 54 89786.5 55 73634.0 56 73634.0 57 73634.0 58 61144.0 59 48654.0 60 48654.0 61 48654.0 62 38213.0 63 27772.0 64 27772.0 65 27772.0 66 20784.0 67 13796.0 68 13796.0 69 13796.0 70 9826.0 71 5856.0 72 5856.0 73 5856.0 74 3976.5 75 2097.0 76 2097.0 77 2097.0 78 1424.0 79 751.0 80 751.0 81 751.0 82 488.0 83 225.0 84 225.0 85 225.0 86 147.5 87 70.0 88 70.0 89 70.0 90 43.5 91 17.0 92 17.0 93 17.0 94 12.0 95 7.0 96 7.0 97 7.0 98 6.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23492778403325132 2 0.14328776996110915 3 0.1453194622217517 4 0.07560033833022697 5 0.02587734353028986 6 0.025342687672226017 7 0.29780331294155893 8 0.2985518311428483 9 0.020637716121264228 10 0.039243739981885864 11 0.0183921615173961 12 0.020209991434813154 13 0.017964436830945025 14 0.021065440807715295 15 0.016895125114817347 16 0.022883270725132354 17 0.021813959009004673 18 0.016360469256753506 19 0.02844369164899629 20 0.01988919791997485 21 0.016895125114817347 22 0.01988919791997485 23 0.02042385377803869 24 0.018071368002557795 25 0.017964436830945025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 935181.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.88715455100304 #Duplication Level Percentage of deduplicated Percentage of total 1 94.02556810054443 78.87537362996233 2 4.519330499153514 7.582275520991052 3 0.6967899560401176 1.753551801957719 4 0.23718676543356157 0.7958769139751074 5 0.13024815896423544 0.5463073720508216 6 0.06966323115055491 0.350631014282931 7 0.05082578891272047 0.29845415667886277 8 0.04065455539035731 0.2728315976986574 9 0.02807189888460678 0.2119384548245825 >10 0.16392751760473245 2.630474045867956 >50 0.01938084497869344 1.1336899323098089 >100 0.014656657537735419 2.4481050158881916 >500 0.0028038813415209632 1.5709864843574346 >1k 8.921440632112155E-4 1.529504059154584 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3183 0.34036191924344056 No Hit CTGTAGGACGTGGAATATGGCAAGA 2406 0.25727639890031984 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2375 0.253961532580324 No Hit GTCCTACAGTGTGCATTTCTCATTT 2056 0.21985048883585104 No Hit CTTTAGGACGTGAAATATGGCGAGG 1787 0.19108600367201645 No Hit CTGTAGGACCTGGAATATGGCGAGA 1426 0.1524838507198072 No Hit GTATCAACGCAGAGTACTTTTTTTT 1073 0.11473714714050007 No Hit CTGAAGGACCTGGAATATGGCGAGA 997 0.1066103780979297 No Hit GTCCTTCAGTGTGCATTTCTCATTT 951 0.10169154420374238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.06931171612768E-4 2 0.0 0.0 0.0 0.0 1.06931171612768E-4 3 0.0 0.0 0.0 0.0 1.06931171612768E-4 4 0.0 0.0 0.0 0.0 1.06931171612768E-4 5 0.0 0.0 0.0 0.0 1.06931171612768E-4 6 0.0 0.0 0.0 0.0 1.06931171612768E-4 7 0.0 0.0 0.0 0.0 1.06931171612768E-4 8 0.0 0.0 0.0 0.0 1.06931171612768E-4 9 0.0 0.0 0.0 0.0 1.06931171612768E-4 10 0.0 0.0 0.0 0.0 1.06931171612768E-4 11 0.0 0.0 0.0 0.0 1.06931171612768E-4 12 0.0 0.0 0.0 0.0 1.06931171612768E-4 13 0.0 0.0 0.0 0.0 1.06931171612768E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 535 0.0 15.482834 4 TGTCGAG 45 6.851948E-4 14.75111 18 CCTACCG 40 0.005155753 14.300011 3 TCAATAC 95 7.0440365E-8 13.045624 3 TGTAGGA 1535 0.0 12.731822 2 GACAGTC 60 4.0282076E-4 12.689285 7 TGGCGAA 60 4.1520922E-4 12.643809 18 GTAGGAC 1450 0.0 12.426216 3 GTATTAG 140 4.9112714E-11 12.269688 1 GGACCTG 910 0.0 12.236097 6 CTGTAGG 1535 0.0 12.185314 1 CTACCGT 55 0.0030164302 12.117704 4 GGCGAAA 55 0.0031086372 12.06909 19 AAGACTC 95 1.0154854E-6 12.022073 5 AGGACCT 980 0.0 11.945403 5 GTATAAG 120 9.340511E-9 11.9288645 1 TTAGTAC 80 2.7626118E-5 11.916676 3 CCTCGAA 65 8.140576E-4 11.671208 17 TAAGACT 100 1.8760275E-6 11.425263 4 ATGGCAA 1090 0.0 11.22283 17 >>END_MODULE