FastQCFastQC Report
Thu 26 May 2016
SRR1926814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935181
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32330.34570847782407893No Hit
CTGTAGGACGTGGAATATGGCAAGA31030.3318074255144191No Hit
GTCCTAAAGTGTGTATTTCTCATTT23300.24914962985774944No Hit
CTTTAGGACGTGAAATATGGCGAGG21880.2339654034887364No Hit
GTCCTACAGTGTGCATTTCTCATTT21390.22872577607971076No Hit
GTATCAACGCAGAGTACTTTTTTTT18380.1965394934242676No Hit
CTGTAGGACCTGGAATATGGCGAGA17520.18734341266556956No Hit
CTGAAGGACCTGGAATATGGCGAGA12460.13323623982950894No Hit
GGTATCAACGCAGAGTACTTTTTTT11040.11805201346049589No Hit
TATCAACGCAGAGTACTTTTTTTTT10100.10800048332889568No Hit
GTACATGGGAAGCAGTGGTATCAAC9690.1036163052927722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1059.094947E-1114.45663519
TCCAACG1850.013.33331518
TAGGACC6850.013.2082364
CCAACGT802.027511E-613.04485319
CGGTTTC1102.7994247E-912.93566213
TTGCACC604.0037587E-412.6984454
CTGTAGG17400.012.3846641
AGGACCT10650.012.2513175
TGTAGGA18100.012.2198082
GACGTGG7400.012.1846837
CCGGTTT1251.4388206E-912.14227512
GTCCTAC15400.012.1355751
CTACCGT550.003009840612.12124354
GTAGGAC17150.012.1067483
TCGAACT550.003098078512.07457519
GGACCTG10100.011.9755136
GGCGAGG6500.011.96842619
TGGCAAG11200.011.94366518
AGGACGT15000.011.9365395
GTTCTAG1603.6379788E-1211.9188671
Produced by FastQC (version 0.11.5)