FastQCFastQC Report
Thu 26 May 2016
SRR1926813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819430
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18670.22784130432129654No Hit
GTCCTAAAGTGTGTATTTCTCATTT15240.18598293936028704No Hit
CTGTAGGACGTGGAATATGGCAAGA13310.16242998181662863No Hit
CTTTAGGACGTGAAATATGGCGAGG11830.14436864649817555No Hit
GTCCTACAGTGTGCATTTCTCATTT11570.14119570921250138No Hit
GTATCAACGCAGAGTACTTTTTTTT10230.1248428785863344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA1250.014.4784064
AGTACGT400.005211581514.2765185
CCAACGT500.001521174713.27350719
GCCGGTT655.558724E-513.12524111
TTAACCT853.821635E-612.3268174
GGCGAGG3200.012.14762919
TTTAACC550.002981127512.1364633
ACTAGAC550.002981127512.1364633
TAGGACC2600.012.0897624
TGTAGGA8050.011.8457062
TCCAACG1157.252129E-811.54218118
AGGACGT7550.011.4716395
TAAGGGT752.021031E-411.430324
CCGGTTT752.1138402E-411.37520912
GGACGTG7250.011.2899366
GTGTAGG1104.6284003E-711.2868981
TAGACAG1451.1750672E-911.1586575
CTATACA1201.2258715E-711.1128114
AGGACCT5500.011.0751165
GAACAGT1553.0195224E-1011.0527886
Produced by FastQC (version 0.11.5)