##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926813_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819430
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.70311435998194	31.0	30.0	33.0	23.0	34.0
2	28.780916002587166	31.0	30.0	33.0	23.0	34.0
3	28.57086389319405	31.0	29.0	33.0	16.0	34.0
4	32.15143941520325	35.0	33.0	37.0	22.0	37.0
5	32.394020233576995	35.0	33.0	37.0	24.0	37.0
6	31.600269699669283	35.0	32.0	37.0	17.0	37.0
7	31.660537202689675	35.0	32.0	37.0	17.0	37.0
8	31.483421402682353	35.0	32.0	37.0	17.0	37.0
9	33.7046959471828	37.0	34.0	39.0	22.0	39.0
10	33.86404451875084	38.0	34.0	39.0	23.0	39.0
11	34.040446407868885	38.0	35.0	39.0	25.0	39.0
12	33.9127576486094	38.0	35.0	39.0	23.0	39.0
13	34.02696874656774	38.0	35.0	39.0	25.0	39.0
14	35.09534554507402	39.0	36.0	41.0	25.0	41.0
15	34.67865101350939	39.0	34.0	41.0	19.0	41.0
16	34.77717803839254	39.0	34.0	41.0	21.0	41.0
17	35.006559437657884	39.0	36.0	41.0	25.0	41.0
18	35.062687477880964	40.0	36.0	41.0	25.0	41.0
19	34.44578548503228	39.0	34.0	41.0	18.0	41.0
20	34.721043896366986	39.0	34.0	41.0	18.0	41.0
21	34.70602980120328	39.0	35.0	41.0	18.0	41.0
22	34.74798823572483	39.0	35.0	41.0	18.0	41.0
23	34.75011898514821	40.0	35.0	41.0	18.0	41.0
24	33.47252236310606	39.0	33.0	41.0	2.0	41.0
25	34.058997107745625	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.2902915211407091
1101	2	-0.30567687279103595
1101	3	-0.9974506543042629
1101	4	-0.4573926015649903
1101	5	-0.27959220918140204
1101	6	-2.182202627379919
1101	7	-0.5234711421654339
1101	8	-0.2190981164307111
1101	9	-0.0412135560148954
1101	10	-9.21557714214217
1101	11	-3.889899593055059
1101	12	-1.6288542411830136
1101	13	-0.7629902729288176
1101	14	-0.6823521536268444
1101	15	-1.9809250866027526
1101	16	-0.33073001533125534
1101	17	-0.09418482898855274
1101	18	-0.09817901159047437
1101	19	-0.8757608506005781
1101	20	-0.1862595882945044
1101	21	-0.29237226015638385
1101	22	0.06765615315428875
1101	23	-0.07236912736108536
1101	24	-0.6629083964457507
1101	25	-0.21576342570667606
1102	1	0.22787051569709504
1102	2	0.0360666289518754
1102	3	-0.019427996474078668
1102	4	-0.03470262975279326
1102	5	0.21776787387579333
1102	6	-0.9149712184200709
1102	7	-0.09003927703654213
1102	8	0.5787243219219178
1102	9	0.5302921514774681
1102	10	0.3902703478522369
1102	11	0.15808153617849285
1102	12	0.24146370541078
1102	13	0.31356799083156517
1102	14	-0.0037482568243305536
1102	15	0.5999420380260716
1102	16	0.6583398140357062
1102	17	0.328305434384518
1102	18	0.11990865570736986
1102	19	0.652346263054369
1102	20	0.500602286086874
1102	21	0.1799819056137224
1102	22	0.3804278104250258
1102	23	0.028159528446465742
1102	24	-3.565080847708824
1102	25	-1.6028367086175521
1103	1	0.12342972622341009
1103	2	-0.11360442367970336
1103	3	0.3169206877364452
1103	4	-0.0832223665948959
1103	5	-0.028942652440001382
1103	6	0.25854851842203175
1103	7	0.3285462492792455
1103	8	0.3821782692903355
1103	9	0.3304566251616805
1103	10	0.35737561101013426
1103	11	0.12600916775743798
1103	12	-0.02087182090500761
1103	13	0.13511404346314038
1103	14	-0.04568904629801551
1103	15	0.2627709853944964
1103	16	0.43136612982517875
1103	17	0.11120017122662063
1103	18	0.05823102412841763
1103	19	-1.6617984737877336
1103	20	-0.27489113496576323
1103	21	0.03688980035056488
1103	22	-0.08667745273287153
1103	23	0.08983716002541087
1103	24	1.713702047028022
1103	25	0.6997948703298178
1104	1	-0.072415421603786
1104	2	-0.0031243038887005525
1104	3	-1.0335868045111205
1104	4	-0.3891181972732909
1104	5	-0.1204359513652804
1104	6	-1.3248584895640327
1104	7	-0.32686678011627635
1104	8	0.1982392108391231
1104	9	0.06103306626443583
1104	10	0.3027707460936
1104	11	0.18832929540707255
1104	12	0.2974796722346227
1104	13	-0.13408650026907054
1104	14	-0.06271851084922275
1104	15	0.3692016542275951
1104	16	-0.2140855555322858
1104	17	-0.11309069364742896
1104	18	-0.15099967974992268
1104	19	-1.1449701211264198
1104	20	-0.25862032021702674
1104	21	-0.2324302157914886
1104	22	-0.1409550959935686
1104	23	-0.17324374138064513
1104	24	1.1937348886660146
1104	25	0.6160015682186071
1105	1	0.02100181919739086
1105	2	-0.20959243113796333
1105	3	0.15262577926962706
1105	4	0.10496889961410005
1105	5	-0.00837152353076931
1105	6	-0.28849598746829486
1105	7	-0.022552095919500914
1105	8	0.34866245227194526
1105	9	-0.4650768946281332
1105	10	0.007622718815881058
1105	11	-0.04662331168997014
1105	12	-0.058210790309196625
1105	13	-0.23777772702588607
1105	14	-0.5178093001330879
1105	15	-0.029620054100377047
1105	16	-0.31629124579987433
1105	17	-0.43582447069218944
1105	18	-0.38604391442569863
1105	19	-1.0257938335639878
1105	20	-0.3114328969794897
1105	21	-0.22649586792640974
1105	22	-0.10157278284559368
1105	23	-0.24807812940589713
1105	24	-4.717797709307263
1105	25	-2.2005873124822877
1106	1	-0.07184707611201091
1106	2	-0.7419150515355177
1106	3	-0.4193370335361877
1106	4	-0.64547380507857
1106	5	-0.08294396399863757
1106	6	-0.14109344722359296
1106	7	-2.8893549395852
1106	8	-0.585220507998546
1106	9	-0.5989730583719179
1106	10	-0.050891968106469676
1106	11	-0.15761274373350886
1106	12	-0.03232680482783934
1106	13	-0.36932833257341
1106	14	-0.4737886612920903
1106	15	0.07051922959825419
1106	16	0.045373893406157606
1106	17	-0.512387787818902
1106	18	-0.3976836822516816
1106	19	0.1575713434402246
1106	20	0.008537744709308015
1106	21	-0.08670344723785917
1106	22	-0.4242980315110074
1106	23	-0.6444981759028323
1106	24	-3.580814262838288
1106	25	-1.6723202295178723
1107	1	0.2309540714565408
1107	2	-0.09635423600767012
1107	3	0.2697276879032664
1107	4	0.09585063782581926
1107	5	0.13530426139768537
1107	6	0.03613761050644371
1107	7	-2.1628273536067333
1107	8	-1.6327195015829652
1107	9	-0.40855413470153223
1107	10	-0.024804147518523223
1107	11	-0.20170138983701946
1107	12	0.13433816324712922
1107	13	-0.2857092111869548
1107	14	-0.03158366845165972
1107	15	-1.193064832855569
1107	16	-0.5578805233569071
1107	17	-0.3925975808794746
1107	18	-0.4500682502063853
1107	19	0.38233833912409665
1107	20	0.350559799223916
1107	21	0.13088742317148672
1107	22	-0.0086267395791495
1107	23	-0.08679871452626031
1107	24	-4.4633808518793145
1107	25	-2.196116225257807
1108	1	0.8534457170827956
1108	2	0.4315743191726682
1108	3	1.0015896795558064
1108	4	0.9699713706228508
1108	5	0.8946442564460142
1108	6	-2.4106187308371183
1108	7	-0.500249166848306
1108	8	0.9191391973340757
1108	9	0.7392174248611667
1108	10	1.0726294039480635
1108	11	0.7744130817354815
1108	12	1.0765211212044292
1108	13	0.7965878111496068
1108	14	0.7290074404084379
1108	15	-0.2893773461301947
1108	16	0.9015189168780608
1108	17	0.8585250282740731
1108	18	0.7780649070116752
1108	19	0.9960219172970355
1108	20	-0.18620447233376325
1108	21	0.362375525965156
1108	22	0.7081282570212935
1108	23	0.7488353444237603
1108	24	0.0021629333337926937
1108	25	0.5968436046259029
1109	1	0.5646207620227344
1109	2	0.5897938438299448
1109	3	-0.6690862196451128
1109	4	0.49107303943186764
1109	5	0.8431399282316505
1109	6	0.7983801445818166
1109	7	1.1770931845777106
1109	8	1.4867216944483914
1109	9	0.9995591787375062
1109	10	0.9078993095896521
1109	11	0.626218287964349
1109	12	0.14147217285474056
1109	13	0.40654437436058544
1109	14	0.599377512943299
1109	15	-0.8685706169538108
1109	16	0.5036999113343299
1109	17	0.32088744029633176
1109	18	0.4496835243336932
1109	19	-0.8977707416258198
1109	20	0.11826255170802114
1109	21	0.47243972234744547
1109	22	0.7249450121657262
1109	23	0.67319273109036
1109	24	-0.1872609322806298
1109	25	0.27077386080522814
1110	1	0.1256843165461241
1110	2	0.1849545962271506
1110	3	0.49355731037173456
1110	4	0.3065055627625597
1110	5	0.2504835829801877
1110	6	0.4052810690134656
1110	7	0.9251394415885237
1110	8	0.9464447192346981
1110	9	0.4441482543913722
1110	10	0.33544986501829044
1110	11	-0.04759428709150626
1110	12	0.1027088368866913
1110	13	0.11550599829148211
1110	14	-0.060229576117080796
1110	15	0.4214623312709307
1110	16	0.15743630354648275
1110	17	-0.1952476608633944
1110	18	-0.08682523437217782
1110	19	-2.56662397970209
1110	20	-0.5813930617678906
1110	21	-0.48195319103549394
1110	22	-0.08648168139379209
1110	23	-0.10795629090810621
1110	24	-0.7764275999653449
1110	25	-0.31893155983133425
1111	1	0.5272518114614861
1111	2	0.19803591371258733
1111	3	0.817582501523411
1111	4	0.8073160350394701
1111	5	0.7036504439635749
1111	6	0.9231707472743302
1111	7	0.8310753008185507
1111	8	1.1806095114522321
1111	9	0.5136153289927421
1111	10	0.3443852240608223
1111	11	0.3453135237184384
1111	12	0.4574621529612486
1111	13	0.343587282671848
1111	14	0.3411218329851238
1111	15	-0.5731162622692452
1111	16	-0.3156092484263695
1111	17	-0.11508704710745121
1111	18	0.09769459466120622
1111	19	0.6403362707407609
1111	20	0.2564246039395357
1111	21	0.27665728689937197
1111	22	0.5168967209796591
1111	23	0.31085894488725785
1111	24	0.6911327430513197
1111	25	0.5602469770088874
1112	1	0.3778846422711979
1112	2	0.5592039392521642
1112	3	-0.8804830497514438
1112	4	0.36181516973057626
1112	5	0.5431533275406295
1112	6	1.4779392594617526
1112	7	1.2716312912553498
1112	8	1.6122648450087738
1112	9	0.33663195612712826
1112	10	0.8116408273485263
1112	11	0.850465294654434
1112	12	0.9219962772545003
1112	13	0.6455677083000779
1112	14	0.7648685497626886
1112	15	1.342137105833629
1112	16	1.259057242191986
1112	17	0.32906221514008394
1112	18	0.7083763260334877
1112	19	1.33792908514026
1112	20	1.125950405234434
1112	21	1.1346658739701994
1112	22	1.128046473647494
1112	23	1.2377020292536969
1112	24	2.32486304961116
1112	25	1.4490229539591368
1113	1	-0.7310110632502749
1113	2	-0.08849222074562135
1113	3	-4.850167151838072
1113	4	-1.3364339902257285
1113	5	-0.19633633150994712
1113	6	0.6934047058948458
1113	7	0.990241428013082
1113	8	1.2292838537341666
1113	9	0.7218323221050156
1113	10	0.8628022540169695
1113	11	0.5685885748118906
1113	12	0.2850541745871027
1113	13	-0.18021080133103595
1113	14	-0.24591258716558428
1113	15	0.3343998309399865
1113	16	0.593970794896947
1113	17	0.36102593539285976
1113	18	0.363877748000462
1113	19	1.1456783441963694
1113	20	0.46650458056272726
1113	21	0.5632327755571751
1113	22	0.8132098500469951
1113	23	0.7075785693338048
1113	24	1.757130637521911
1113	25	1.1166998240646393
1114	1	0.6428491191205445
1114	2	0.6272270432855827
1114	3	0.7602972474868857
1114	4	0.6479956385242787
1114	5	0.844652572324037
1114	6	1.496947874525059
1114	7	1.5213678978154768
1114	8	1.4544642035408586
1114	9	0.7226338768014173
1114	10	0.7923385169451791
1114	11	0.424028984586748
1114	12	-0.08223200327582703
1114	13	0.3663170509819409
1114	14	0.42685784619918365
1114	15	-1.6487606695407138
1114	16	0.060397984728396636
1114	17	0.28829683576029197
1114	18	0.5039597075390745
1114	19	1.150446567005737
1114	20	1.0347743185616736
1114	21	0.9272773383444886
1114	22	0.9657541582093785
1114	23	0.5183186029945333
1114	24	1.520860401691273
1114	25	1.041112759467552
1115	1	-3.6576777299169407
1115	2	-1.115743799898027
1115	3	-0.23268823039220976
1115	4	0.5836806548475977
1115	5	0.7060890239732558
1115	6	1.273661822515603
1115	7	1.0816346995716408
1115	8	1.2379056084716211
1115	9	0.7655833308146995
1115	10	-0.3299940283660874
1115	11	0.24906106834223607
1115	12	0.0845911420579526
1115	13	0.38959747426235936
1115	14	0.3482132441503296
1115	15	0.7570144064471265
1115	16	0.5290549699811251
1115	17	0.38994042463792766
1115	18	0.406613090405223
1115	19	-1.78080188509378
1115	20	-0.3067308035817433
1115	21	-0.1459805700198089
1115	22	0.21295217689675638
1115	23	0.0571714680176143
1115	24	-1.2037236352331888
1115	25	-0.5495728197286667
1116	1	0.07454449230528226
1116	2	-0.5342322883865158
1116	3	-0.2974329751369531
1116	4	-0.2251982240312813
1116	5	0.11582876371299733
1116	6	0.9672053660591438
1116	7	0.6436467115836528
1116	8	-1.7082205176545031
1116	9	-0.41740966217829367
1116	10	-0.5604344688065268
1116	11	-0.12559358631241935
1116	12	0.08291947038628678
1116	13	-0.05249461782972986
1116	14	-0.139224193287113
1116	15	-0.959952560519838
1116	16	-0.2397037887776321
1116	17	-0.15280231810481837
1116	18	-0.019372895580765714
1116	19	0.5671360528441554
1116	20	0.2313272544763123
1116	21	0.3520174279781898
1116	22	0.21061984456442673
1116	23	0.2785027993489422
1116	24	1.3109810694715165
1116	25	0.6675743274184853
1201	1	0.3404782984518526
1201	2	0.17126959647707096
1201	3	0.4794157885203205
1201	4	0.3070533892841567
1201	5	0.36076199481644267
1201	6	0.6197138319718682
1201	7	0.5941642503990643
1201	8	0.9321845682824943
1201	9	0.376615639719347
1201	10	0.4110619212004991
1201	11	0.46177974446516856
1201	12	0.7360962642013789
1201	13	0.49774562241051257
1201	14	0.5174961440715222
1201	15	1.1514824915535087
1201	16	0.6866593829046863
1201	17	0.5545582339365893
1201	18	0.4578502850466748
1201	19	0.32479307156500425
1201	20	0.6741360206893319
1201	21	0.7537515024782238
1201	22	0.5718331855649978
1201	23	0.8195040133288813
1201	24	-0.36941315453972834
1201	25	0.32068722755266066
1202	1	0.15253324054719286
1202	2	-0.20244797242498436
1202	3	-0.5289188914329941
1202	4	-0.6606887778762669
1202	5	-0.11310460003049627
1202	6	0.3216224413622939
1202	7	0.31257703431144535
1202	8	0.6143894340693734
1202	9	0.6118468094832394
1202	10	0.47435665142763384
1202	11	0.24537611756867506
1202	12	0.38171256728064407
1202	13	0.2972392932965846
1202	14	0.40058080158117804
1202	15	0.9380270646749764
1202	16	0.5495519226299734
1202	17	0.4363070444222643
1202	18	0.32465340920503394
1202	19	0.01679773984761823
1202	20	0.3757070773371467
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2211	1	0.6297436537308556
2211	2	0.5230961721494936
2211	3	0.2257184782031807
2211	4	0.8469650713395609
2211	5	0.7653063545113454
2211	6	-1.9520404835640655
2211	7	0.29211687986514434
2211	8	0.6440272017630235
2211	9	0.7699998227783595
2211	10	0.882808207832376
2211	11	0.6485159949669708
2211	12	0.3948357262648017
2211	13	0.33170788656145334
2211	14	0.6298205117953302
2211	15	1.1468257272018434
2211	16	0.34168949129489334
2211	17	0.42556586139544095
2211	18	0.2257186110201772
2211	19	0.5748934328656929
2211	20	0.3989854787383109
2211	21	0.25382376261471506
2211	22	-2.463092547072492
2211	23	-0.9202149750292037
2211	24	1.0096795793021016
2211	25	0.5547458385522575
2212	1	-0.6292146560646472
2212	2	-0.27833417470397137
2212	3	-2.905399474570519
2212	4	-1.3777287485677938
2212	5	-0.49198039274885375
2212	6	0.0126505118036917
2212	7	0.25048034162087873
2212	8	0.19202403827867442
2212	9	-0.00747277918751621
2212	10	0.03439164473216039
2212	11	-0.051361420014387704
2212	12	-0.3326233179109934
2212	13	-0.3414459943559578
2212	14	-0.4655647554061133
2212	15	-1.3822071504391005
2212	16	-0.48332915066647075
2212	17	-0.37632149596531406
2212	18	-0.22852441236700827
2212	19	0.49740614329388677
2212	20	-0.6766698144488998
2212	21	-0.7853407385715911
2212	22	-0.27904272219394954
2212	23	-0.1559278888242588
2212	24	1.1116544973904539
2212	25	0.4526460758135826
2213	1	-0.8840493228151658
2213	2	-0.8322668195418288
2213	3	-0.2519047011765707
2213	4	-7.7480378303292525
2213	5	-2.1876282942451866
2213	6	-3.6693822718413216
2213	7	-1.4376117787145937
2213	8	-3.366748888485951
2213	9	-1.4446647867702538
2213	10	-0.7099679107255668
2213	11	-0.7362135836540986
2213	12	-0.6883043040257846
2213	13	-0.6218942875669811
2213	14	-0.8158916709673392
2213	15	-2.9672916700720045
2213	16	-1.516988763174389
2213	17	-0.8924162756482588
2213	18	-0.7928962313696672
2213	19	-0.21083828308216823
2213	20	-1.6164004432138768
2213	21	-0.737350634758215
2213	22	-0.9161973327028647
2213	23	-0.6788114165924242
2213	24	-0.8644315899996613
2213	25	-0.7636229989401357
2214	1	0.12940411212553116
2214	2	0.01036187628638885
2214	3	-0.08999038580460095
2214	4	0.3557339966647177
2214	5	0.18595275312832626
2214	6	0.28922692233267
2214	7	0.638176495483517
2214	8	-2.6253219426299452
2214	9	-1.29322946910046
2214	10	-0.8527215662117342
2214	11	-0.41920108070731743
2214	12	-1.630526437941512
2214	13	-0.7673366968450352
2214	14	-0.7110592710277004
2214	15	-2.156013510517795
2214	16	-0.7918559767565938
2214	17	-0.6251368573684886
2214	18	-0.40658079066267305
2214	19	0.31045292017677184
2214	20	-1.5642105883056274
2214	21	-0.7956531488262399
2214	22	-0.49093657943294033
2214	23	-0.766228940264682
2214	24	-1.8526766827689158
2214	25	-1.011088766598938
2215	1	0.738630487590985
2215	2	0.33496957624329227
2215	3	0.5863429002263416
2215	4	1.1412378902102418
2215	5	0.9448053262301599
2215	6	1.8501634887758591
2215	7	1.2207389830797304
2215	8	0.15231997895036642
2215	9	0.5170542457676888
2215	10	0.4121458291212434
2215	11	0.207635399806108
2215	12	0.1550151578806549
2215	13	0.42786998232272566
2215	14	0.4182584905671902
2215	15	0.2401892418531233
2215	16	0.6295497002637092
2215	17	0.13930507824544947
2215	18	-0.40249742464530414
2215	19	0.587331632476463
2215	20	-3.285670357620056
2215	21	-1.6307674783619461
2215	22	-0.5031002764344095
2215	23	-0.41022781129095875
2215	24	-0.272689470124412
2215	25	0.05125299511324499
2216	1	-1.0853010040786764
2216	2	-1.76544575036921
2216	3	-0.8789538451193621
2216	4	-1.0461330641968907
2216	5	-1.293317068509758
2216	6	-2.1410982037828887
2216	7	-10.270074702137759
2216	8	-11.096065463191756
2216	9	-6.542129796513812
2216	10	-4.202122014340226
2216	11	-3.153795604899056
2216	12	-2.6329280339227594
2216	13	-2.298201123151621
2216	14	-2.429131704348464
2216	15	-3.44490867047595
2216	16	-2.5994799850922945
2216	17	-2.9546574276522364
2216	18	-2.6015712181636985
2216	19	-1.7707306600225508
2216	20	-2.727563589029913
2216	21	-5.1764977736139315
2216	22	-3.7937780444488496
2216	23	-3.561731665885521
2216	24	-3.70048453814794
2216	25	-3.2567753180850083
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40154.0
3	7.0
4	303.0
5	1847.0
6	2521.0
7	1923.0
8	1676.0
9	1443.0
10	1432.0
11	1316.0
12	1325.0
13	1296.0
14	1375.0
15	1411.0
16	1468.0
17	1607.0
18	1783.0
19	2270.0
20	3022.0
21	3831.0
22	4709.0
23	5805.0
24	6455.0
25	7802.0
26	8973.0
27	9707.0
28	10912.0
29	12990.0
30	15693.0
31	18939.0
32	24879.0
33	32968.0
34	45229.0
35	63884.0
36	94243.0
37	154562.0
38	229670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94750955089099	18.743967616882966	12.451450402036544	25.857072430189497
2	14.053561935952978	20.53916327041873	42.11412615100726	23.293148642621027
3	18.663003707768226	24.729494233039464	30.05650863638697	26.55099342280534
4	11.92334278602955	16.957208841079904	39.12170529570818	31.99774307718236
5	12.465501436092365	37.993401176948794	36.867719597625594	12.673377789333246
6	30.664228667404743	37.72018587709994	19.349869575990752	12.26571587950457
7	27.05217212492717	31.309394997233742	23.205603999079546	18.43282887875954
8	23.93304753174882	37.65935600409733	21.996849900320274	16.410746563833577
9	26.000161119438324	15.507867999238345	20.52795910495711	37.96401177636622
10	13.915299345639223	29.661221799003805	35.6303105772048	20.793168278152162
11	33.937790948880874	22.63243590275625	25.214267754747333	18.21550539361554
12	22.8944294518065	24.977572071461786	32.61222203099997	19.515776445731735
13	28.730901519090544	22.480733694126492	25.965512275023006	22.822852511759955
14	20.07416249838273	22.773355206143915	28.05407661830725	29.098405677166106
15	22.54808026783611	32.068443208711756	23.267590780989302	22.115885742462837
16	21.34553603687176	28.470520169125763	28.951556992061246	21.232386801941235
17	21.06467043502991	29.10932444094	28.502325831190184	21.323679292839905
18	20.818274912151583	28.206207914230163	30.456640901051983	20.51887627256627
19	23.25957807287477	27.25229816378819	27.56783412939538	21.92028963394166
20	22.79683969881202	28.14977376725429	28.582710130369136	20.470676403564553
21	22.23861815261397	27.313504428732017	27.484380130085228	22.96349728856878
22	22.230359309356594	28.99984620905316	27.688229133864034	21.081565347726215
23	21.572633799103613	28.471062678813798	28.087192782025365	21.86911074005722
24	22.79296619923569	27.461213888424403	28.694704388252912	21.051115524086995
25	22.07450931091764	27.89259703627578	28.45819698843047	21.574696664376113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	290.0
1	290.0
2	362.0
3	434.0
4	434.0
5	434.0
6	640.5
7	847.0
8	847.0
9	847.0
10	1274.5
11	1702.0
12	1702.0
13	1702.0
14	2893.5
15	4085.0
16	4085.0
17	4085.0
18	7267.0
19	10449.0
20	10449.0
21	10449.0
22	16459.0
23	22469.0
24	22469.0
25	22469.0
26	32124.5
27	41780.0
28	41780.0
29	41780.0
30	55066.5
31	68353.0
32	68353.0
33	68353.0
34	76504.0
35	84655.0
36	84655.0
37	84655.0
38	90694.0
39	96733.0
40	96733.0
41	96733.0
42	101209.5
43	105686.0
44	105686.0
45	105686.0
46	106515.0
47	107344.0
48	107344.0
49	107344.0
50	101803.5
51	96263.0
52	96263.0
53	96263.0
54	83877.5
55	71492.0
56	71492.0
57	71492.0
58	60393.0
59	49294.0
60	49294.0
61	49294.0
62	39637.5
63	29981.0
64	29981.0
65	29981.0
66	22920.0
67	15859.0
68	15859.0
69	15859.0
70	11652.5
71	7446.0
72	7446.0
73	7446.0
74	5070.0
75	2694.0
76	2694.0
77	2694.0
78	1877.5
79	1061.0
80	1061.0
81	1061.0
82	725.0
83	389.0
84	389.0
85	389.0
86	238.0
87	87.0
88	87.0
89	87.0
90	57.5
91	28.0
92	28.0
93	28.0
94	16.0
95	4.0
96	4.0
97	4.0
98	4.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24004490926619726
2	0.13729055563013315
3	0.1400973847674603
4	0.07517420646058845
5	0.023552957543658395
6	0.021844452851372296
7	0.2980120327544757
8	0.28226938237555377
9	0.01976984001073917
10	0.037831175329192245
11	0.015620614329472926
12	0.016963010873412007
13	0.015132470131676897
14	0.01806133531845307
15	0.01501043408222789
16	0.019281695812943145
17	0.018671515565698106
18	0.014766361983329875
19	0.023064813345862367
20	0.01720708297231002
21	0.014766361983329875
22	0.016596902725064984
23	0.01732911902175903
24	0.015742650378921932
25	0.01501043408222789
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	819430.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.94433022598942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.1621149998586	79.1359346109959
2	5.214719036493947	8.859216317433903
3	0.8227537616488858	2.0966480167253385
4	0.28712904862576283	0.9755993889576388
5	0.13496589174972437	0.5732293639016863
6	0.08352428044331062	0.42569484359188364
7	0.05251589956296506	0.3122649540213978
8	0.035589969473423466	0.24185328957466923
9	0.025318207261825494	0.19355743425406932
>10	0.15189570832824453	2.3970417437484137
>50	0.014234346128423513	0.8651771671659887
>100	0.013513697624684847	2.4590960878468686
>500	8.625764001136418E-4	0.47736039936523533
>1k	8.625764001136418E-4	0.9873263824169679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1867	0.22784130432129654	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1524	0.18598293936028704	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1331	0.16242998181662863	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1183	0.14436864649817555	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1157	0.14119570921250138	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1023	0.1248428785863344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.2203604944900723E-4	0.0
8	0.0	0.0	0.0	1.2203604944900723E-4	0.0
9	0.0	0.0	0.0	1.2203604944900723E-4	0.0
10	0.0	0.0	0.0	1.2203604944900723E-4	0.0
11	0.0	0.0	0.0	1.2203604944900723E-4	0.0
12	0.0	0.0	0.0	1.2203604944900723E-4	0.0
13	0.0	0.0	0.0	1.2203604944900723E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	125	0.0	14.478406	4
AGTACGT	40	0.0052115815	14.276518	5
CCAACGT	50	0.0015211747	13.273507	19
GCCGGTT	65	5.558724E-5	13.125241	11
TTAACCT	85	3.821635E-6	12.326817	4
GGCGAGG	320	0.0	12.147629	19
TTTAACC	55	0.0029811275	12.136463	3
ACTAGAC	55	0.0029811275	12.136463	3
TAGGACC	260	0.0	12.089762	4
TGTAGGA	805	0.0	11.845706	2
TCCAACG	115	7.252129E-8	11.542181	18
AGGACGT	755	0.0	11.471639	5
TAAGGGT	75	2.021031E-4	11.43032	4
CCGGTTT	75	2.1138402E-4	11.375209	12
GGACGTG	725	0.0	11.289936	6
GTGTAGG	110	4.6284003E-7	11.286898	1
TAGACAG	145	1.1750672E-9	11.158657	5
CTATACA	120	1.2258715E-7	11.112811	4
AGGACCT	550	0.0	11.075116	5
GAACAGT	155	3.0195224E-10	11.052788	6
>>END_MODULE