FastQCFastQC Report
Thu 26 May 2016
SRR1926813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819430
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19060.2326007102498078No Hit
CTGTAGGACGTGGAATATGGCAAGA17660.21551566332694677No Hit
GTATCAACGCAGAGTACTTTTTTTT16640.20306798628314804No Hit
CTTTAGGACGTGAAATATGGCGAGG15170.18512868701414398No Hit
GTCCTAAAGTGTGTATTTCTCATTT14900.1818337136790208No Hit
GTCCTACAGTGTGCATTTCTCATTT11370.13875498822352123No Hit
GGTATCAACGCAGAGTACTTTTTTT9830.1199614366083741No Hit
TATCAACGCAGAGTACTTTTTTTTT9410.11483592253151581No Hit
CTGTAGGACCTGGAATATGGCGAGA8730.10653747116898332No Hit
CTGAAGGACCTGGAATATGGCGAGA8540.10421878622945217No Hit
GTACATGGGAAGCAGTGGTATCAAC8210.10019159659763494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4400.015.7846074
CCAACGA602.5874293E-514.23704719
GTAGGAC10200.013.0616413
TGTAGGA11300.012.8140692
CTGTAGG11350.012.6791241
TCCAACG751.4904337E-512.65515218
GTCTAGT701.0577233E-412.2532871
GGACCTA701.0754078E-412.2315496
AGGACCT7900.012.0430515
TGGCGAG10550.011.96541718
GGACCTG7000.011.8238316
GTCCTAA4850.011.7901051
GGACGTG9400.011.7399756
AGGACGT9500.011.7172555
GGCGAGA6800.011.30588919
GACGTGG4800.011.272377
TCTAGTA855.168139E-511.207352
TAGGACG9950.011.1873274
TAAGTAG600.00580756811.0996785
GACGTGA5050.011.0902747