##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926813_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 819430 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.720506937749413 31.0 30.0 33.0 25.0 34.0 2 30.06169654515944 31.0 30.0 33.0 26.0 34.0 3 30.142605225583637 31.0 30.0 34.0 26.0 34.0 4 33.74195965488205 35.0 35.0 37.0 28.0 37.0 5 33.696399936541255 37.0 35.0 37.0 28.0 37.0 6 33.621285527744895 37.0 35.0 37.0 29.0 37.0 7 33.623435802936186 37.0 35.0 37.0 28.0 37.0 8 33.64741100521094 37.0 35.0 37.0 29.0 37.0 9 35.2040088842244 39.0 35.0 39.0 29.0 39.0 10 35.08399741283575 39.0 35.0 39.0 29.0 39.0 11 35.13023931269297 39.0 35.0 39.0 29.0 39.0 12 35.02404720354393 39.0 35.0 39.0 28.0 39.0 13 35.037677409906884 39.0 35.0 39.0 28.0 39.0 14 36.12619503801423 40.0 36.0 41.0 28.0 41.0 15 36.10942972554093 40.0 36.0 41.0 28.0 41.0 16 36.091423306444725 40.0 36.0 41.0 28.0 41.0 17 36.066813516712834 40.0 36.0 41.0 28.0 41.0 18 36.04026213343422 40.0 36.0 41.0 27.0 41.0 19 36.105606336111684 40.0 37.0 41.0 27.0 41.0 20 36.06976434838852 40.0 37.0 41.0 27.0 41.0 21 36.01079652929475 40.0 37.0 41.0 27.0 41.0 22 35.92964133605067 40.0 36.0 41.0 27.0 41.0 23 35.853316329643775 40.0 36.0 41.0 27.0 41.0 24 35.786189180283856 40.0 36.0 41.0 27.0 41.0 25 35.66896501226462 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0232084826743737 1101 2 0.024369172194440125 1101 3 0.338317487803657 1101 4 0.07778547585934348 1101 5 0.1337182967863626 1101 6 0.02337398655973999 1101 7 0.07825431490942236 1101 8 -0.07030948249939684 1101 9 0.0039939211091564175 1101 10 -0.07422831733909163 1101 11 0.1376302756007135 1101 12 0.049759471252897924 1101 13 0.16769448055983815 1101 14 0.13912809939527193 1101 15 0.05479303665158852 1101 16 0.16110269731527183 1101 17 0.12131927150338839 1101 18 0.19447488045783956 1101 19 0.013802432121437391 1101 20 0.11632048315794918 1101 21 0.1861296260736367 1101 22 0.10575316031690107 1101 23 0.20159758307687525 1101 24 0.07005413538305305 1101 25 -0.03286853212389218 1102 1 -0.46262891130113104 1102 2 -0.38555021821740354 1102 3 -0.18528240138169494 1102 4 -0.21191548596877396 1102 5 -0.22673459084973757 1102 6 -0.04463245252448189 1102 7 0.053382881419729244 1102 8 -0.024364437958361407 1102 9 0.1728255527741709 1102 10 0.08531115634512076 1102 11 0.023140184366901906 1102 12 0.08718496722914182 1102 13 0.07346706307909301 1102 14 0.26964260750732905 1102 15 0.2414767200014154 1102 16 0.201001350325555 1102 17 -0.08498559788594662 1102 18 -0.11681073085170368 1102 19 -0.013555606782034602 1102 20 -0.1771083695338831 1102 21 -0.1186523487940292 1102 22 -0.004411259573792847 1102 23 -0.19458630468832894 1102 24 -0.3799474784225154 1102 25 -0.2925197618437352 1103 1 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9 0.49984276647006 1107 10 0.47648483638515415 1107 11 0.5560792326611761 1107 12 0.5815834783643439 1107 13 0.6978779814209091 1107 14 0.5689990488285446 1107 15 0.5891730458000666 1107 16 0.5292084568398607 1107 17 0.6947630258524811 1107 18 0.5801901658035575 1107 19 0.55256489951541 1107 20 0.5302405544797963 1107 21 0.5564013462597686 1107 22 0.46373792926597446 1107 23 0.5262098417792629 1107 24 0.6141877705557093 1107 25 0.5109055134085807 1108 1 0.675187253814272 1108 2 0.5240355585262009 1108 3 0.6428947710795931 1108 4 0.6748741859707934 1108 5 0.5691784793952692 1108 6 0.7970730391487919 1108 7 0.6002050568026718 1108 8 0.5323835481846331 1108 9 0.4464577212703489 1108 10 0.46264717829929225 1108 11 0.39880147031372815 1108 12 0.2656473926225189 1108 13 0.44865749256279486 1108 14 0.5616029616539961 1108 15 0.504064630598684 1108 16 0.5082606265891769 1108 17 0.5264326061292479 1108 18 0.47598451310672374 1108 19 0.48725437067946586 1108 20 0.5307036739570847 1108 21 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0.16491997912559953 2214 5 0.22647458859493952 2214 6 0.10069011368883451 2214 7 0.05590002341573097 2214 8 0.015462446719332945 2214 9 0.033130647337706876 2214 10 -0.1866119966519051 2214 11 -0.045796305479761656 2214 12 -0.09459436253765574 2214 13 -0.02721155275182241 2214 14 -0.01863667045672912 2214 15 -0.11774816980003777 2214 16 -0.09637931500993346 2214 17 -0.1722885120252684 2214 18 -0.17467791805737676 2214 19 -0.09658479526864028 2214 20 0.010670658835181257 2214 21 0.02118324816185435 2214 22 0.11005100448829808 2214 23 0.07777834889265023 2214 24 0.04992339148668634 2214 25 0.06806671575864698 2215 1 0.5839892113647132 2215 2 0.5049340105971325 2215 3 0.6068340512806856 2215 4 0.5234743900563288 2215 5 0.49872429915141936 2215 6 0.6374610917330159 2215 7 0.4572999298931393 2215 8 0.457419530008373 2215 9 0.3480116835638398 2215 10 0.5658594661283587 2215 11 0.34566381467529794 2215 12 0.42175716720603873 2215 13 0.5311177965330671 2215 14 0.4294810695640834 2215 15 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>>Per sequence quality scores pass #Quality Count 2 21825.0 3 22.0 4 369.0 5 2338.0 6 3146.0 7 2228.0 8 1741.0 9 1455.0 10 1346.0 11 1212.0 12 1110.0 13 1087.0 14 1073.0 15 1012.0 16 1080.0 17 1151.0 18 1348.0 19 1571.0 20 1973.0 21 2474.0 22 3201.0 23 3930.0 24 4652.0 25 5395.0 26 6568.0 27 7194.0 28 8141.0 29 10189.0 30 12982.0 31 16632.0 32 21597.0 33 28549.0 34 38654.0 35 53622.0 36 79530.0 37 139194.0 38 329839.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54256085969589 19.54987159783121 10.493914641268042 28.413652901204856 2 14.032483312990026 21.211776880961718 40.27962578089586 24.47611402515239 3 18.392151406190116 25.001647736010725 29.595535733901617 27.010665123897542 4 11.928535689442661 17.728054867407835 38.31272958031803 32.03067986283148 5 12.680518688024408 38.69107551487414 35.73247902364607 12.895926773455377 6 31.211456981215253 38.43476770101149 17.899267713257082 12.454507604516177 7 27.685626085608504 31.84374317203363 22.208727346409667 18.261903395948202 8 24.474427744112674 38.65270429428384 20.113871446094137 16.758996515509338 9 26.165264321858274 15.675139319112166 20.040471500301464 38.11912485872811 10 14.397557204209477 30.14880600056872 34.608836584332956 20.844800210888845 11 34.10155177358629 22.808081671009354 24.778950066208193 18.311416489196166 12 23.02052302784527 25.213901034999104 32.14403829045143 19.621537646704198 13 28.90652744573276 22.59679747918337 25.73732299915672 22.759352075927154 14 20.281317844499036 23.023959366827388 27.648032959536454 29.04668982913712 15 22.25849519894948 32.22171236654934 23.261541315395093 22.25825111910608 16 21.367979272967354 28.773036307698312 28.512850052415573 21.346134366918758 17 20.950351649880464 29.393365819607208 28.290638656713323 21.365643873799005 18 20.940503678247328 28.25916632902618 30.367507432158668 20.43282256056782 19 23.130572525511887 27.25811608670502 27.59347442465543 22.017836963127667 20 22.949093136836144 28.245779321420812 28.334866942146743 20.470260599596298 21 22.15494388001699 27.40664163765873 27.319373721494518 23.119040760829765 22 22.466647466281355 29.078210654261266 27.363844047311055 21.091297832146328 23 21.58654767294707 28.70664500925647 27.819073572142482 21.887733745653975 24 22.62644826988648 27.426149524179124 28.757935432851777 21.18946677308262 25 22.124601223849194 28.07908887429795 28.27838541730231 21.51792448455055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 323.0 1 323.0 2 313.5 3 304.0 4 304.0 5 304.0 6 524.5 7 745.0 8 745.0 9 745.0 10 1177.0 11 1609.0 12 1609.0 13 1609.0 14 2672.0 15 3735.0 16 3735.0 17 3735.0 18 6752.0 19 9769.0 20 9769.0 21 9769.0 22 15519.0 23 21269.0 24 21269.0 25 21269.0 26 30638.5 27 40008.0 28 40008.0 29 40008.0 30 53148.0 31 66288.0 32 66288.0 33 66288.0 34 74734.5 35 83181.0 36 83181.0 37 83181.0 38 89279.0 39 95377.0 40 95377.0 41 95377.0 42 100152.5 43 104928.0 44 104928.0 45 104928.0 46 107345.0 47 109762.0 48 109762.0 49 109762.0 50 104432.5 51 99103.0 52 99103.0 53 99103.0 54 85903.0 55 72703.0 56 72703.0 57 72703.0 58 61899.0 59 51095.0 60 51095.0 61 51095.0 62 41007.0 63 30919.0 64 30919.0 65 30919.0 66 23663.0 67 16407.0 68 16407.0 69 16407.0 70 11929.0 71 7451.0 72 7451.0 73 7451.0 74 5170.0 75 2889.0 76 2889.0 77 2889.0 78 1978.0 79 1067.0 80 1067.0 81 1067.0 82 710.0 83 353.0 84 353.0 85 353.0 86 232.5 87 112.0 88 112.0 89 112.0 90 68.5 91 25.0 92 25.0 93 25.0 94 15.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11080873289969857 2 0.11947329241057808 3 0.015132470131676897 4 0.0 5 0.006711982719695398 6 0.3429212989517103 7 0.023064813345862367 8 0.010373064203165614 9 0.010739172351512637 10 0.005003478027409297 11 0.0054916222252053256 12 0.0010983244450410651 13 0.001586468642837094 14 1.2203604944900723E-4 15 0.003172937285674188 16 0.002074612840633123 17 8.542523461430506E-4 18 0.0021966488900821302 19 9.762883955920579E-4 20 0.0014644325933880868 21 0.013912109637186825 22 2.4407209889801447E-4 23 3.661081483470217E-4 24 4.8814419779602894E-4 25 0.005857730373552347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 819430.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.47673658657534 #Duplication Level Percentage of deduplicated Percentage of total 1 91.62211135960744 75.56692744112216 2 6.424087592066319 10.596755602798819 3 1.0269305682397594 2.5409364590823835 4 0.3274744030154761 1.0803608030541374 5 0.16191218086798745 0.6676994145803473 6 0.09934820657563333 0.4916349518452309 7 0.064614582218495 0.37304399141004985 8 0.04266582159617839 0.2815150183230258 9 0.03181540724579197 0.23616278665252238 >10 0.16495919757810654 2.503407808710189 >50 0.01644874564454254 0.9567119105482891 >100 0.015261255049826117 2.6421669880118928 >500 0.0014816751840559918 0.9041854268467993 >1k 8.89005110433595E-4 1.1584913970141448 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1906 0.2326007102498078 No Hit CTGTAGGACGTGGAATATGGCAAGA 1766 0.21551566332694677 No Hit GTATCAACGCAGAGTACTTTTTTTT 1664 0.20306798628314804 No Hit CTTTAGGACGTGAAATATGGCGAGG 1517 0.18512868701414398 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1490 0.1818337136790208 No Hit GTCCTACAGTGTGCATTTCTCATTT 1137 0.13875498822352123 No Hit GGTATCAACGCAGAGTACTTTTTTT 983 0.1199614366083741 No Hit TATCAACGCAGAGTACTTTTTTTTT 941 0.11483592253151581 No Hit CTGTAGGACCTGGAATATGGCGAGA 873 0.10653747116898332 No Hit CTGAAGGACCTGGAATATGGCGAGA 854 0.10421878622945217 No Hit GTACATGGGAAGCAGTGGTATCAAC 821 0.10019159659763494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 440 0.0 15.784607 4 CCAACGA 60 2.5874293E-5 14.237047 19 GTAGGAC 1020 0.0 13.061641 3 TGTAGGA 1130 0.0 12.814069 2 CTGTAGG 1135 0.0 12.679124 1 TCCAACG 75 1.4904337E-5 12.655152 18 GTCTAGT 70 1.0577233E-4 12.253287 1 GGACCTA 70 1.0754078E-4 12.231549 6 AGGACCT 790 0.0 12.043051 5 TGGCGAG 1055 0.0 11.965417 18 GGACCTG 700 0.0 11.823831 6 GTCCTAA 485 0.0 11.790105 1 GGACGTG 940 0.0 11.739975 6 AGGACGT 950 0.0 11.717255 5 GGCGAGA 680 0.0 11.305889 19 GACGTGG 480 0.0 11.27237 7 TCTAGTA 85 5.168139E-5 11.20735 2 TAGGACG 995 0.0 11.187327 4 TAAGTAG 60 0.005807568 11.099678 5 GACGTGA 505 0.0 11.090274 7 >>END_MODULE