FastQCFastQC Report
Thu 26 May 2016
SRR1926812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815729
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24010.2943379480195016No Hit
CTGTAGGACGTGGAATATGGCAAGA18840.23095905625520238No Hit
GTCCTAAAGTGTGTATTTCTCATTT18570.227649133474475No Hit
CTTTAGGACGTGAAATATGGCGAGG15180.1860912141164529No Hit
GTCCTACAGTGTGCATTTCTCATTT14090.17272893326092367No Hit
GTACATGGGAAGCAGTGGTATCAAC13550.16610908769946883No Hit
GTATCAACGCAGAGTACTTTTTTTT11860.14539142288676754No Hit
CCCATGTACTCTGCGTTGATACCAC11440.1402426541167471No Hit
CTGTAGGACCTGGAATATGGCGAGA9950.12197678395643652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3450.014.3493664
GCCGGTT759.847918E-713.90909611
CGGTTTC701.1075276E-412.19303913
TGTAGGA12050.012.1827112
GTGTAGA1103.58923E-812.1413491
TTAGTAC550.002988656712.1323683
GTAGGAC11650.011.8645783
CTGTAGG11750.011.8534981
CCTACAC1459.094947E-1111.8335413
TCCAACG1052.7755414E-711.74144518
GTCTAAA657.7410095E-411.7410851
ATAGGAC1406.4028427E-1011.575273
AGGACCT6600.011.5340195
GTCCTAA4400.011.4909191
TAGGACA2600.011.351074
CTATAGG1354.4128683E-911.306231
GACTGCC600.005800451611.1014957
GGACATG3000.011.1014946
GGCGAGG4700.011.09768319
AATGTCC1803.6379788E-1211.0933048
Produced by FastQC (version 0.11.5)