##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926812_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815729 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.311891571833293 31.0 28.0 31.0 16.0 34.0 2 28.375373438972993 31.0 28.0 31.0 16.0 34.0 3 28.15013319374449 31.0 28.0 33.0 16.0 34.0 4 31.677707915251265 35.0 32.0 37.0 19.0 37.0 5 31.93942718721536 35.0 33.0 37.0 19.0 37.0 6 31.08629091279089 35.0 32.0 37.0 15.0 37.0 7 31.176450757543254 35.0 32.0 37.0 17.0 37.0 8 31.00209996211977 35.0 32.0 37.0 11.0 37.0 9 33.179633187002054 37.0 33.0 39.0 17.0 39.0 10 33.3475333106951 37.0 34.0 39.0 17.0 39.0 11 33.52594427806294 38.0 34.0 39.0 19.0 39.0 12 33.395069931312975 38.0 34.0 39.0 17.0 39.0 13 33.495108056719815 38.0 34.0 39.0 18.0 39.0 14 34.52722656666614 39.0 34.0 41.0 18.0 41.0 15 34.1046315626881 39.0 33.0 41.0 16.0 41.0 16 34.197419731307825 39.0 34.0 41.0 17.0 41.0 17 34.42401704487642 39.0 34.0 41.0 17.0 41.0 18 34.4848117450771 39.0 34.0 41.0 17.0 41.0 19 33.84550996715821 39.0 33.0 41.0 10.0 41.0 20 34.13771975741944 39.0 34.0 41.0 11.0 41.0 21 34.13207082254033 39.0 34.0 41.0 10.0 41.0 22 34.15452435796692 39.0 34.0 41.0 10.0 41.0 23 34.16020761797117 39.0 34.0 41.0 2.0 41.0 24 32.856658277442634 39.0 32.0 41.0 2.0 41.0 25 33.439934095759746 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0026288520478949806 1101 2 -0.04729717295793989 1101 3 -0.8625622637648505 1101 4 -0.5791401656734294 1101 5 -0.10898268179210291 1101 6 -1.9502646681799192 1101 7 -0.5194366315135781 1101 8 -0.1559680239013268 1101 9 -0.1340187657167604 1101 10 -9.096384604570215 1101 11 -3.742722255692538 1101 12 -1.3352955281226144 1101 13 -0.6909688418941684 1101 14 -0.4527286929497478 1101 15 -1.8694349495166662 1101 16 -0.2535598762396347 1101 17 -0.009640761832670819 1101 18 0.18425088176571336 1101 19 -0.7032084478932319 1101 20 -0.010806607934000567 1101 21 -0.25493500725929863 1101 22 0.18390579424684006 1101 23 0.006375462507854479 1101 24 -0.2839450406484474 1101 25 0.18223787144874137 1102 1 0.1738763339389422 1102 2 0.04495669528602875 1102 3 0.01716320878240296 1102 4 0.12070928355721122 1102 5 0.39417566975733465 1102 6 -0.7145964585018092 1102 7 0.2507644957420041 1102 8 0.7865628363275512 1102 9 0.6743263545095459 1102 10 0.5500782560015551 1102 11 0.2521397305060802 1102 12 0.5209713485650553 1102 13 0.3187231987384678 1102 14 0.15666974689423796 1102 15 0.7623371562653389 1102 16 0.7406859769171064 1102 17 0.6596645615568093 1102 18 0.36514754286039164 1102 19 0.5876197420686182 1102 20 0.5674851498132085 1102 21 0.47486386548511916 1102 22 0.352152976107881 1102 23 0.06622609042779004 1102 24 -3.060020862516353 1102 25 -1.5344266521464789 1103 1 0.21482736781783984 1103 2 0.016404125743360254 1103 3 0.44913532436130765 1103 4 0.24977912931729662 1103 5 0.017172234719954105 1103 6 0.45783366206126885 1103 7 0.2449464853695389 1103 8 0.26339654010609337 1103 9 0.08904245877303651 1103 10 0.17102726927022616 1103 11 0.025580156181305824 1103 12 0.04942154052302783 1103 13 0.012305740666128884 1103 14 0.18201156679051422 1103 15 0.44701217478760213 1103 16 0.2857536673596215 1103 17 0.0073954162749316765 1103 18 -0.021760249959704936 1103 19 -1.6176008412141982 1103 20 -0.4532463110879874 1103 21 -0.21401200169343326 1103 22 0.005473512243128198 1103 23 -0.05057258944020049 1103 24 1.2809436420973768 1103 25 0.9188426951964175 1104 1 -0.3211468944985505 1104 2 -0.1284866840837573 1104 3 -1.2664418236953665 1104 4 -0.6774371712156579 1104 5 -0.2021303780448953 1104 6 -1.5929046714887107 1104 7 -0.15317908332927743 1104 8 0.21057904368077018 1104 9 0.08743255366449887 1104 10 0.24325266898425468 1104 11 0.07357768640358131 1104 12 -0.044654242656484655 1104 13 -0.17416637684329572 1104 14 -0.059344211189468865 1104 15 0.14199072671792834 1104 16 -0.15231080683770415 1104 17 -0.11699558853463543 1104 18 -0.34830971050825354 1104 19 -1.2063776513012883 1104 20 -0.4243954076692944 1104 21 -0.11163841531620733 1104 22 -0.1706313883158188 1104 23 0.07465034049610608 1104 24 1.326982248622791 1104 25 0.67038825609594 1105 1 0.06757288591458277 1105 2 -0.1524040826228621 1105 3 0.3851506586718294 1105 4 -0.08516280325683923 1105 5 0.07436470286743457 1105 6 -0.11096101603096287 1105 7 0.08210512783205104 1105 8 0.5538915226294883 1105 9 -0.3299540459061845 1105 10 0.057602982428420546 1105 11 -0.055944254688014894 1105 12 0.1340083034049755 1105 13 -0.03552828797295149 1105 14 -0.28286609698217546 1105 15 0.3979378710329513 1105 16 -0.14408509687535798 1105 17 -0.18276356444442854 1105 18 -0.20263295263193726 1105 19 -0.9086911557930719 1105 20 -0.16317978724087823 1105 21 -0.08733706652599693 1105 22 -0.1389847130359314 1105 23 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9 -0.6620525598795837 1107 10 -0.1845531564484446 1107 11 -0.6403447944441112 1107 12 -0.3535628790512533 1107 13 -0.502884077196498 1107 14 -0.3325813671572604 1107 15 -1.7115833924715602 1107 16 -0.7371166420544668 1107 17 -0.6215402870386484 1107 18 -0.7914508358714869 1107 19 0.015921212768681414 1107 20 0.07973591406013725 1107 21 -0.22368064334364135 1107 22 -0.21640491654774507 1107 23 -0.3026770441252964 1107 24 -4.267728730077302 1107 25 -2.2789058235659247 1108 1 0.5913374772712068 1108 2 -0.024898590087950367 1108 3 0.5337247953892863 1108 4 0.5701841383069848 1108 5 0.8198685095204503 1108 6 -2.740954465245295 1108 7 -1.1706728566485651 1108 8 0.5815304706489428 1108 9 0.406001106219648 1108 10 0.6779660447804332 1108 11 0.43595204527697007 1108 12 0.7238498280667898 1108 13 0.659251455582698 1108 14 0.5728263161833169 1108 15 -0.5012696912818555 1108 16 0.22945363266259022 1108 17 0.20333144756534693 1108 18 0.23288694431212775 1108 19 0.39826011831644337 1108 20 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25 -0.9091391980836718 2215 1 0.5682267157226839 2215 2 0.10146752388368085 2215 3 0.5099498395164836 2215 4 0.8340956369854524 2215 5 0.8843845279212594 2215 6 1.4988631271017212 2215 7 0.9199845242537315 2215 8 -0.43117839045784834 2215 9 0.17568114975238558 2215 10 -0.17336531616631135 2215 11 -0.11153613412179908 2215 12 -0.23231677610539947 2215 13 0.056456809377635864 2215 14 0.10751875191308358 2215 15 0.15848904956117593 2215 16 0.1501710082604788 2215 17 -0.11628892136544522 2215 18 -0.7828205083825992 2215 19 0.4839937729628332 2215 20 -3.6464113877695468 2215 21 -2.0752733227899185 2215 22 -0.8593586336329722 2215 23 -0.6202767314520301 2215 24 -0.7457620697731713 2215 25 -0.4415902235332041 2216 1 -1.6627754938179216 2216 2 -2.177847718940324 2216 3 -1.474476281161511 2216 4 -1.6730396510314236 2216 5 -1.8317928415730513 2216 6 -3.116474516756405 2216 7 -10.813396323388172 2216 8 -11.872576087725733 2216 9 -7.047802484115213 2216 10 -5.187402287141666 2216 11 -4.145202819159554 2216 12 -3.739166477241234 2216 13 -3.309805234121754 2216 14 -3.6271090331852953 2216 15 -4.7635883194321025 2216 16 -3.6045198815180157 2216 17 -3.826825323672189 2216 18 -3.5703814461096286 2216 19 -3.1144416368042407 2216 20 -4.0951685838074035 2216 21 -6.793744155350833 2216 22 -5.2897311742038475 2216 23 -5.240852013656962 2216 24 -5.576019498789801 2216 25 -4.932388188350902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46904.0 3 3.0 4 331.0 5 2130.0 6 2901.0 7 2357.0 8 1834.0 9 1667.0 10 1617.0 11 1442.0 12 1505.0 13 1412.0 14 1460.0 15 1548.0 16 1615.0 17 1751.0 18 1930.0 19 2479.0 20 3246.0 21 4125.0 22 5099.0 23 6045.0 24 7196.0 25 8228.0 26 9399.0 27 10092.0 28 11479.0 29 13776.0 30 16383.0 31 19938.0 32 25732.0 33 34316.0 34 45925.0 35 64741.0 36 93878.0 37 152203.0 38 209042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41062268316867 19.15402866020954 12.90729234372101 25.52805631290078 2 13.734140545211721 20.25370217613324 42.88963685285568 23.122520425799355 3 18.741253590631217 24.658491075593528 30.047015786501678 26.553239547273577 4 11.969665185992106 16.98150935572869 38.966889074535466 32.08193638374374 5 12.305358787256035 38.25580383035023 36.80768179081564 12.631155591578086 6 30.73883108841327 37.54287010526703 19.388997541521313 12.329301264798387 7 27.186877750190515 31.23269991887706 22.946606357088424 18.633815973843998 8 23.805415343925098 38.19745812393766 21.76768663714541 16.229439894991835 9 25.886103498801443 15.778877220086688 20.76119009521007 37.5738291859018 10 14.262589001684992 29.650590973641027 35.274712607028896 20.81210741764508 11 33.5877890673948 22.836155601357042 25.257449451138246 18.318605880109907 12 23.28622172414512 25.00585472197121 32.42522815023891 19.282695403644766 13 28.714460344064452 22.867007600511524 25.759836587163115 22.658695468260905 14 19.879032060304958 23.417338165838633 27.88172459624222 28.821905177614187 15 22.44700285675752 32.63079167739483 23.15089319650323 21.771312269344417 16 20.811957428024915 28.66104271911325 29.554293982049145 20.972705870812693 17 20.728058795977272 29.608639889352915 28.815816255214173 20.847485059455643 18 20.59935655363851 28.222749163813987 31.084908436405723 20.092985846141772 19 23.297752981208426 27.268814567303274 27.75978664050765 21.67364581098066 20 23.048609280455135 28.326906453649837 28.655014498795346 19.96946976709969 21 22.03065439444636 27.522011159759145 27.47076111224864 22.97657333354585 22 22.186583487822343 29.41642389822362 27.62116352293794 20.775829091016096 23 21.077425224443154 28.973091344255703 28.401959846684665 21.547523584616478 24 22.853236880823932 27.523295733202552 29.026606179499755 20.59686120647376 25 21.859340535384337 28.03772923279888 28.796923986115758 21.306006245701024 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 323.0 1 323.0 2 397.0 3 471.0 4 471.0 5 471.0 6 714.0 7 957.0 8 957.0 9 957.0 10 1333.5 11 1710.0 12 1710.0 13 1710.0 14 2914.0 15 4118.0 16 4118.0 17 4118.0 18 7270.0 19 10422.0 20 10422.0 21 10422.0 22 16543.5 23 22665.0 24 22665.0 25 22665.0 26 32963.5 27 43262.0 28 43262.0 29 43262.0 30 56585.5 31 69909.0 32 69909.0 33 69909.0 34 78405.5 35 86902.0 36 86902.0 37 86902.0 38 92453.5 39 98005.0 40 98005.0 41 98005.0 42 100973.5 43 103942.0 44 103942.0 45 103942.0 46 106300.5 47 108659.0 48 108659.0 49 108659.0 50 101816.0 51 94973.0 52 94973.0 53 94973.0 54 81706.0 55 68439.0 56 68439.0 57 68439.0 58 57880.0 59 47321.0 60 47321.0 61 47321.0 62 37828.0 63 28335.0 64 28335.0 65 28335.0 66 21536.5 67 14738.0 68 14738.0 69 14738.0 70 10732.0 71 6726.0 72 6726.0 73 6726.0 74 4630.5 75 2535.0 76 2535.0 77 2535.0 78 1733.0 79 931.0 80 931.0 81 931.0 82 606.0 83 281.0 84 281.0 85 281.0 86 179.5 87 78.0 88 78.0 89 78.0 90 47.0 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 5.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22654582588089917 2 0.13153878310076017 3 0.13595201347506342 4 0.06865025026693915 5 0.023292049197711495 6 0.02255651080199429 7 0.263445335399379 8 0.254741464383392 9 0.020104716149603605 10 0.03616397112276259 11 0.016181844705778513 12 0.01814328042769106 13 0.015446306310061306 14 0.01728515229935432 15 0.014588178181724568 16 0.020717664812701278 17 0.01998212641698407 18 0.014833357646963636 19 0.025008305454384976 20 0.017652921497212922 21 0.014710767914344103 22 0.016794793368876182 23 0.019001408556027798 24 0.015814075507919908 25 0.015691485775300378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 815729.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.56187320691328 #Duplication Level Percentage of deduplicated Percentage of total 1 92.62438044683692 76.47242354320647 2 5.58000085222884 9.21390645712371 3 0.9041105850412632 2.2393519046161483 4 0.291692784313657 0.9633081069550261 5 0.1526247339442216 0.6300491966070848 6 0.08659233395854862 0.42895351781858293 7 0.063146721530824 0.3649458131522106 8 0.04593749267638733 0.30341483566331184 9 0.03616875833848064 0.2687544395993814 >10 0.17896716031763557 2.800667042786971 >50 0.01737342496263866 1.0474409077853903 >100 0.01648064343605628 2.9706796262868784 >500 0.0013362683370958686 0.7326793681276935 >1k 0.00118779407741855 1.5634252402711397 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2401 0.2943379480195016 No Hit CTGTAGGACGTGGAATATGGCAAGA 1884 0.23095905625520238 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1857 0.227649133474475 No Hit CTTTAGGACGTGAAATATGGCGAGG 1518 0.1860912141164529 No Hit GTCCTACAGTGTGCATTTCTCATTT 1409 0.17272893326092367 No Hit GTACATGGGAAGCAGTGGTATCAAC 1355 0.16610908769946883 No Hit GTATCAACGCAGAGTACTTTTTTTT 1186 0.14539142288676754 No Hit CCCATGTACTCTGCGTTGATACCAC 1144 0.1402426541167471 No Hit CTGTAGGACCTGGAATATGGCGAGA 995 0.12197678395643652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 345 0.0 14.349366 4 GCCGGTT 75 9.847918E-7 13.909096 11 CGGTTTC 70 1.1075276E-4 12.193039 13 TGTAGGA 1205 0.0 12.182711 2 GTGTAGA 110 3.58923E-8 12.141349 1 TTAGTAC 55 0.0029886567 12.132368 3 GTAGGAC 1165 0.0 11.864578 3 CTGTAGG 1175 0.0 11.853498 1 CCTACAC 145 9.094947E-11 11.833541 3 TCCAACG 105 2.7755414E-7 11.741445 18 GTCTAAA 65 7.7410095E-4 11.741085 1 ATAGGAC 140 6.4028427E-10 11.57527 3 AGGACCT 660 0.0 11.534019 5 GTCCTAA 440 0.0 11.490919 1 TAGGACA 260 0.0 11.35107 4 CTATAGG 135 4.4128683E-9 11.30623 1 GACTGCC 60 0.0058004516 11.101495 7 GGACATG 300 0.0 11.101494 6 GGCGAGG 470 0.0 11.097683 19 AATGTCC 180 3.6379788E-12 11.093304 8 >>END_MODULE