FastQCFastQC Report
Thu 26 May 2016
SRR1926812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815729
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA23720.2907828457735351No Hit
GTCCTACAGTGGACATTTCTAAATT23040.28244674395540675No Hit
GTATCAACGCAGAGTACTTTTTTTT20160.2471409009609809No Hit
CTTTAGGACGTGAAATATGGCGAGG19530.23941774780595027No Hit
GTCCTAAAGTGTGTATTTCTCATTT18280.2240940312285085No Hit
GTCCTACAGTGTGCATTTCTCATTT15100.1851104962554966No Hit
GTACATGGGAAGCAGTGGTATCAAC14690.1800843172180957No Hit
CCCATGTACTCTGCGTTGATACCAC13370.1639024725123172No Hit
GGTATCAACGCAGAGTACTTTTTTT12740.15617931935728654No Hit
CTGTAGGACCTGGAATATGGCGAGA12670.15532119122894983No Hit
CTGAAGGACCTGGAATATGGCGAGA11790.1445332947584308No Hit
TATCAACGCAGAGTACTTTTTTTTT10580.12969993711146716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCCG508.7945125E-515.181951511
TAGGACC4300.014.6115554
CCCCTAG400.005153639214.3005831
ACGTTTT400.005318003214.23308119
GGCGAGG4550.013.55531519
CACGTTT604.1294962E-412.65162818
AAATGTC2350.012.5178037
GTAGCAC550.003016414412.1174083
AGGACCT7850.012.0056725
GGACCTG7550.011.9783676
CTGTAGG12550.011.9266471
ATGTCCA2250.011.8089099
TAGGACA3650.011.7365544
GTCCTAC11950.011.6478941
CACTGTT2200.011.6459627
TGTCCAC2950.011.57945510
CAGGACT1655.456968E-1211.5389684
TGTAGGA12850.011.4976942
GTTCTAA1001.8452956E-611.4404661
TCTATAC1001.8756145E-611.4249843
Produced by FastQC (version 0.11.5)