##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926811_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42519 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.82685387709024 30.0 10.0 31.0 2.0 34.0 2 22.94362520285049 30.0 10.0 31.0 2.0 34.0 3 22.83967167619182 30.0 10.0 31.0 2.0 34.0 4 25.636844704720243 35.0 10.0 37.0 2.0 37.0 5 25.771702062607304 35.0 10.0 37.0 2.0 37.0 6 25.027305439920976 33.0 8.0 37.0 2.0 37.0 7 25.039088407535456 33.0 8.0 37.0 2.0 37.0 8 24.880359368752792 33.0 2.0 37.0 2.0 37.0 9 26.48201980291164 35.0 2.0 39.0 2.0 39.0 10 26.623674122157155 35.0 2.0 39.0 2.0 39.0 11 26.61560714033726 35.0 2.0 39.0 2.0 39.0 12 26.523507137985373 35.0 2.0 39.0 2.0 39.0 13 26.58442108233966 35.0 2.0 39.0 2.0 39.0 14 27.362802511818245 37.0 2.0 40.0 2.0 41.0 15 26.978574284437546 36.0 2.0 40.0 2.0 41.0 16 26.99491991815424 36.0 2.0 40.0 2.0 41.0 17 27.128765963451634 37.0 2.0 40.0 2.0 41.0 18 27.151743926244738 37.0 2.0 40.0 2.0 41.0 19 26.64051365271996 36.0 2.0 40.0 2.0 41.0 20 26.778569580658058 37.0 2.0 40.0 2.0 41.0 21 26.75552106117265 37.0 2.0 40.0 2.0 41.0 22 26.731273077918107 37.0 2.0 40.0 2.0 41.0 23 26.693736917613304 37.0 2.0 40.0 2.0 41.0 24 25.671605635127825 36.0 2.0 40.0 2.0 41.0 25 26.085679343352382 36.0 2.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7936054005490725 1101 2 2.2793680862974774 1101 3 2.09638630954872 1101 4 1.952681571161719 1101 5 3.100242426491505 1101 6 1.6755826513084138 1101 7 2.467777819242123 1101 8 2.541785607125842 1101 9 3.5172899801937554 1101 10 -5.311282387223816 1101 11 -0.21231685991228488 1101 12 1.3965503005084052 1101 13 2.051589108842208 1101 14 2.336509244615936 1101 15 1.8444582754831167 1101 16 2.503889354285313 1101 17 2.8387219689855954 1101 18 4.262408239628638 1101 19 3.893845852093346 1101 20 3.2521728127127894 1101 21 3.5777563546011244 1101 22 4.207958847914501 1101 23 4.960715529799376 1101 24 4.8723131754437965 1101 25 4.6460854521297215 1102 1 2.258188733882406 1102 2 2.3335347529641446 1102 3 2.8995113095487213 1102 4 3.686014904495053 1102 5 3.8148257598248385 1102 6 2.508915984641746 1102 7 3.0709028192421215 1102 8 3.47407727379251 1102 9 3.0797899801937554 1102 10 1.952259279442849 1102 11 2.9022664734210473 1102 12 3.0371753005084052 1102 13 2.8109641088422066 1102 14 2.8865092446159366 1102 15 3.4361249421497817 1102 16 3.1278476876186474 1102 17 3.044971968985596 1102 18 2.4738665729619704 1102 19 2.9521791854266795 1102 20 3.068839479379456 1102 21 3.120464687934458 1102 22 3.575667181247834 1102 23 2.329465529799375 1102 24 -0.2391451578895385 1102 25 0.47629378546305645 1103 1 -1.0118672362668484 1103 2 -1.5853085306179437 1103 3 -0.8782405561229218 1103 4 -1.847567185057187 1103 5 -1.3582152849512816 1103 6 -0.8567556571493 1103 7 -1.2702818822504156 1103 8 -1.537816382923907 1103 9 -1.5274861392092305 1103 10 -1.9976007951840167 1103 11 -2.2558491982207443 1103 12 -2.7527034308348775 1103 13 -3.608112383695108 1103 14 -3.079908665831823 1103 15 -2.520964610089024 1103 16 -2.953075819844038 1103 17 -3.5291884787755983 1103 18 -3.611721113605192 1103 19 -3.956402904125561 1103 20 -3.229667983307113 1103 21 -3.2463729986327046 1103 22 -2.6885585650208235 1103 23 -2.594508350797639 1103 24 -2.4819156802775986 1103 25 -3.0705812145369435 1104 1 0.08408159102526369 1104 2 0.41587602280541347 1104 3 -1.7401216269592155 1104 4 0.0550625235426736 1104 5 -1.192614716365636 1104 6 -1.7529887772630168 1104 7 0.3082540097183113 1104 8 0.5576586229988578 1104 9 0.6284010913048661 1104 10 0.10379697785554853 1104 11 1.0853021877067626 1104 12 0.5552804592385634 1104 13 0.08650974376284282 1104 14 -0.3793637712570792 1104 15 0.5214424024672404 1104 16 -0.6000788996829414 1104 17 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0.7614193683487613 1109 3 -0.23438292122050797 1109 4 1.0206302891104393 1109 5 1.4681911444402225 1109 6 1.2858390615648219 1109 7 1.0562393577036602 1109 8 1.4546061199463551 1109 9 1.4711361340399094 1109 10 1.6515381255966943 1109 11 1.2017857041902786 1109 12 1.5503964543545585 1109 13 1.9131275703806665 1109 14 1.5903553984620906 1109 15 0.4765095575343956 1109 16 1.387222687618646 1109 17 1.8579527382163654 1109 18 1.5726646498850485 1109 19 -0.3958977376502446 1109 20 1.268839479379455 1109 21 1.9687819956267667 1109 22 1.4797537197093718 1109 23 1.037638606722453 1109 24 -0.6610201578895385 1109 25 0.4371110931553659 1110 1 1.139982212143277 1110 2 1.0445854776018244 1110 3 1.2253718167950964 1110 4 0.4215221508718656 1110 5 0.9357496728683152 1110 6 0.9944232310185583 1110 7 -0.1430917459752692 1110 8 0.7301914042272912 1110 9 1.0680146178749155 1110 10 0.6227755837906734 1110 11 0.18261067631959804 1110 12 0.4255358077547804 1110 13 0.9878209929001791 1110 14 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1.8269854350921264 2208 15 2.8389027199275603 2208 16 1.9396036399996 2208 17 2.6664997467633746 2208 18 2.5465352237556225 2208 19 1.1367029949504897 2208 20 1.4180458285858037 2208 21 1.4991849260296952 2208 22 2.2619271018827547 2208 23 1.789286958370802 2208 24 1.815170318300936 2208 25 1.0246568807011514 2209 1 2.6115541184977893 2209 2 2.4229578298872205 2209 3 1.5040786172410279 2209 4 2.2821687506489 2209 5 2.314344990594069 2209 6 3.5166082923340554 2209 7 1.7485470500113536 2209 8 1.5315291968694318 2209 9 3.024982287886065 2209 10 2.5130765871351564 2209 11 2.8787087811133567 2209 12 2.84270414666225 2209 13 3.3592814165345146 2209 14 3.4980477061543986 2209 15 3.0457403267651664 2209 16 2.479530379926338 2209 17 2.9502604305240574 2209 18 2.726510803731202 2209 19 0.3425638008112948 2209 20 2.714993325533303 2209 21 3.399551226395996 2209 22 3.4643691043247564 2209 23 2.653023222107066 2209 24 1.2620567651873849 2209 25 2.4063418623861317 2210 1 -1.4542206864074494 2210 2 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0.35569869788330166 2211 14 0.8556873268077183 2211 15 1.6227687777662183 2211 16 1.6995514547419361 2211 17 1.395571284054089 2211 18 0.9231388332359423 2211 19 0.7381380895362675 2211 20 0.19212715061233254 2211 21 0.6994458523180214 2211 22 -1.9189817913549057 2211 23 -0.8310652921184349 2211 24 -0.16786947295803145 2211 25 -0.15277299535886257 2212 1 -0.4587755518318808 2212 2 0.03174903867843071 2212 3 -3.0321851190227065 2212 4 -1.4782708097906614 2212 5 -0.8164242401751629 2212 6 -0.3767983010725402 2212 7 -0.18222218075787922 2212 8 0.33702370236393975 2212 9 -0.011281448377673087 2212 10 0.27681285087141916 2212 11 -0.2718406694360951 2212 12 -0.10344969949159477 2212 13 0.5087319659850635 2212 14 -0.03491932681263421 2212 15 -1.5817322007073606 2212 16 0.30150840190436057 2212 17 -1.0255637453001185 2212 18 -0.032829855609456615 2212 19 0.4271791854266809 2212 20 -0.8025890920491143 2212 21 -0.8674817406369719 2212 22 0.08414932410497755 2212 23 0.37500124408509095 2212 24 1.6675512706818907 2212 25 1.0579902140344863 2213 1 -1.7589767590753418 2213 2 -2.3610075005569833 2213 3 -1.9726277749583225 2213 4 -6.211872419448607 2213 5 -1.8713538176399496 2213 6 -3.1178445787385343 2213 7 -1.8681376737156263 2213 8 -3.087322374094814 2213 9 -3.0320057944541325 2213 10 -2.087573467036023 2213 11 -2.234617329395853 2213 12 -2.349928572731031 2213 13 -1.5343263841155412 2213 14 -1.6874344173558953 2213 15 -3.8402835085544424 2213 16 -2.1536223828038885 2213 17 -1.970432960591868 2213 18 -0.9774978636577494 2213 19 -0.8840884202071244 2213 20 -1.7198929149867404 2213 21 -1.0910229881218818 2213 22 -1.34884866382259 2213 23 -0.9773126392147091 2213 24 -1.2244004395796786 2213 25 -1.3381868483397596 2214 1 1.132344139287813 2214 2 0.8352239421533341 2214 3 0.19638630954872127 2214 4 1.0981770666572146 2214 5 0.7160081922572701 2214 6 0.5494565251822863 2214 7 1.0096697111340127 2214 8 -2.25100718566695 2214 9 -0.5637911008873253 2214 10 0.2026815767401473 2214 11 0.8845299869345595 2214 12 -0.8196659157078123 2214 13 0.6272647845178838 2214 14 0.6229957311024243 2214 15 -0.29022640920156917 2214 16 0.4872226876186474 2214 17 1.0508841311477575 2214 18 0.44979562701602305 2214 19 1.0974494556969496 2214 20 0.33910974964972596 2214 21 1.0313599582047281 2214 22 -0.3478970079413557 2214 23 0.387742556826403 2214 24 -0.7691282659976473 2214 25 -0.38139202534775407 2215 1 0.7945063014499745 2215 2 0.4838725908019832 2215 3 0.20989982306223354 2215 4 0.7197986882788356 2215 5 1.3106027868518666 2215 6 1.4683754441012056 2215 7 0.9421021435664443 2215 8 0.573317138657373 2215 9 0.47674943965321503 2215 10 1.1891680632266315 2215 11 0.9926380950426683 2215 12 0.1938475978057035 2215 13 0.8029404601935575 2215 14 0.35272546083215417 2215 15 1.4124762935011326 2215 16 1.298033498429458 2215 17 1.023857104120733 2215 18 0.8416875189079178 2215 19 0.8406926989401917 2215 20 -0.14737673683676178 2215 21 0.8421707690155387 2215 22 1.0439948839505355 2215 23 1.4282830973669434 2215 24 0.9876284907591106 2215 25 0.9429322989765687 2216 1 -0.5617936604837901 2216 2 -0.9807258104161356 2216 3 -0.16981087354987068 2216 4 -0.21187241944860702 2216 5 -0.3643115641188217 2216 6 -0.31502767733008596 2216 7 -8.023067251180414 2216 8 -7.79154772620749 2216 9 -4.862991709947089 2216 10 -2.735460790979687 2216 11 -2.192363808269093 2216 12 -2.800632798083143 2216 13 -2.6751714545380736 2216 14 -2.5184203328488515 2216 15 -3.6431004099628943 2216 16 -2.6184111151982528 2216 17 -2.759165354958064 2216 18 -2.4141175819676057 2216 19 -1.9545109554183924 2216 20 -3.1283436192120924 2216 21 -4.752994819107794 2216 22 -4.546031762414138 2216 23 -5.216749258933021 2216 24 -4.0694708621148905 2216 25 -4.3663558624242675 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 9631.0 3 1.0 4 52.0 5 277.0 6 322.0 7 207.0 8 192.0 9 182.0 10 135.0 11 131.0 12 154.0 13 169.0 14 142.0 15 160.0 16 160.0 17 161.0 18 172.0 19 233.0 20 261.0 21 324.0 22 343.0 23 410.0 24 422.0 25 459.0 26 491.0 27 510.0 28 523.0 29 621.0 30 667.0 31 758.0 32 1032.0 33 1299.0 34 1699.0 35 2278.0 36 3603.0 37 5503.0 38 8835.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.69870570761723 20.984510927222576 12.676521206120187 26.640262159040006 2 14.70671378091873 22.169611307420496 38.447585394581864 24.676089517078918 3 17.734222243162385 25.293882070249 30.511908407736342 26.45998727885227 4 13.114098067371296 18.846072361761728 36.74583931640028 31.293990254466703 5 13.064239173899747 37.1627502175805 36.14894267635782 13.624067932161926 6 29.665263108371953 37.51734845098916 18.983321963727036 13.834066476911858 7 25.997216521595547 32.500648692001036 22.45181987592291 19.050314910480502 8 23.157845079724503 36.196811019907535 22.516275120294367 18.12906878007359 9 24.524840045163717 17.054008280015054 20.968197214904027 37.4529544599172 10 13.572520879896482 30.770497588518996 34.81472767909658 20.842253852487943 11 31.463317103003785 25.084091924822992 23.87740220638393 19.57518876578929 12 21.27584503563616 27.497471361701127 30.694140615811634 20.53254298685108 13 26.99880036694658 24.162021028861762 25.648625126431916 23.190553477759742 14 19.96800978524216 24.750076447204382 27.445722484886975 27.836191282666476 15 22.776562463246535 31.675017053607128 24.15966880718839 21.38875167595794 16 20.90419401124362 30.63533507397737 27.445722484886975 21.014748429892034 17 20.680732952273424 30.176651847670122 27.481005809987536 21.66160939006892 18 20.111022251493626 30.077151056122688 28.414169450063508 21.397657242320175 19 21.913297109119565 29.26398983840237 28.276056735586764 20.546656316891305 20 21.485192764566133 29.19107096652789 28.294874508973727 21.028861759932255 21 21.84273045891845 28.861759932256014 27.958506809681744 21.337002799143793 22 20.875967351163176 29.609766424387836 28.31839672570743 21.195869498741562 23 20.965351774751255 29.5250864441465 27.68564909557077 21.823912685531486 24 22.449603650648037 28.440712252722694 28.007903464822526 21.10178063180674 25 20.96770399642462 29.02406322771858 28.12316232681768 21.88507044903912 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 149.5 3 123.0 4 123.0 5 123.0 6 127.5 7 132.0 8 132.0 9 132.0 10 138.0 11 144.0 12 144.0 13 144.0 14 244.0 15 344.0 16 344.0 17 344.0 18 532.5 19 721.0 20 721.0 21 721.0 22 1049.5 23 1378.0 24 1378.0 25 1378.0 26 1790.0 27 2202.0 28 2202.0 29 2202.0 30 2713.0 31 3224.0 32 3224.0 33 3224.0 34 3743.5 35 4263.0 36 4263.0 37 4263.0 38 4515.5 39 4768.0 40 4768.0 41 4768.0 42 5095.5 43 5423.0 44 5423.0 45 5423.0 46 5422.0 47 5421.0 48 5421.0 49 5421.0 50 5136.5 51 4852.0 52 4852.0 53 4852.0 54 4359.0 55 3866.0 56 3866.0 57 3866.0 58 3246.5 59 2627.0 60 2627.0 61 2627.0 62 2059.5 63 1492.0 64 1492.0 65 1492.0 66 1145.0 67 798.0 68 798.0 69 798.0 70 572.5 71 347.0 72 347.0 73 347.0 74 244.5 75 142.0 76 142.0 77 142.0 78 95.5 79 49.0 80 49.0 81 49.0 82 35.5 83 22.0 84 22.0 85 22.0 86 13.0 87 4.0 88 4.0 89 4.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2398927538277006 2 0.1622803922952092 3 0.16463228203861802 4 0.0893718102495355 5 0.014111338460452974 6 0.018815117947270633 7 0.29633810766951246 8 0.28928243843928597 9 0.016463228203861802 10 0.032926456407723605 11 0.014111338460452974 12 0.014111338460452974 13 0.014111338460452974 14 0.014111338460452974 15 0.014111338460452974 16 0.014111338460452974 17 0.014111338460452974 18 0.011759448717044145 19 0.014111338460452974 20 0.014111338460452974 21 0.014111338460452974 22 0.014111338460452974 23 0.014111338460452974 24 0.014111338460452974 25 0.014111338460452974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 42519.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.3385545285637 #Duplication Level Percentage of deduplicated Percentage of total 1 97.04714211707822 92.52334250570333 2 2.6173619162740214 4.990710035513535 3 0.20721809704714209 0.5926762153390249 4 0.03700323161556109 0.14111338460452974 5 0.014801292646224437 0.07055669230226487 6 0.022201938969336657 0.12700204614407679 7 0.009867528430816291 0.06585291281544721 8 0.0049337642154081455 0.037630235894541265 9 0.0024668821077040728 0.02116700769067946 >10 0.029602585292448875 0.5973799948258426 >50 0.0 0.0 >100 0.007400646323112219 0.8325689691667255 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 134 0.31515322561678305 No Hit GTATCAACGCAGAGTACTTTTTTTT 114 0.2681154307486065 No Hit GGTATCAACGCAGAGTACTTTTTTT 106 0.24930031280133588 No Hit TATCAACGCAGAGTACTTTTTTTTT 44 0.10348314870998847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE