FastQCFastQC Report
Thu 26 May 2016
SRR1926810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735097
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11680.15889059539081238No Hit
CTGTAGGACGTGGAATATGGCAAGA9320.12678598878787425No Hit
GTCCTAAAGTGTGTATTTCTCATTT8290.11277423251625296No Hit
GTCCTACAGTGTGCATTTCTCATTT8230.11195801370431385No Hit
GTATCAACGCAGAGTACTTTTTTTT7930.10787691964461833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG1155.0367817E-912.4344311
TAGTACC701.0695202E-412.2383854
TAAGACA1501.2732926E-1112.05707554
GTATAAT1209.473297E-911.916331
TAGGACC3000.011.7397844
GTCCTAC6500.011.7330031
GTCCTAG1302.4647306E-911.7330021
GGCGAGG1800.011.59364519
GGGCTAT600.005720729511.1219081
GCCTAGG600.005720729511.1219081
CCTCTAT600.005720729511.1219081
TCCAACG951.38021605E-510.98345418
TGTAGGA6550.010.9082622
TCAATAC700.001455482910.88748553
TGTAGTG1158.623083E-710.7551785
GTAGGAC6300.010.7362713
GCACCAT803.7139474E-410.7034716
ACTGATC803.7398006E-410.6954418
GTCTAGA1002.3035613E-510.486371
CTGTAGG6600.010.3997071
Produced by FastQC (version 0.11.5)