##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926810_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 735097 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.190267406886438 31.0 27.0 31.0 16.0 31.0 2 27.857278699273703 31.0 28.0 31.0 16.0 34.0 3 27.673945071194687 31.0 28.0 31.0 15.0 34.0 4 31.348570324732655 35.0 32.0 37.0 17.0 37.0 5 31.113070792017925 35.0 32.0 37.0 16.0 37.0 6 30.8017295676625 35.0 32.0 37.0 11.0 37.0 7 30.897405376433316 35.0 32.0 37.0 11.0 37.0 8 30.52418388321541 35.0 30.0 37.0 10.0 37.0 9 32.686265894160904 37.0 32.0 39.0 17.0 39.0 10 32.8126954674009 37.0 33.0 39.0 16.0 39.0 11 32.99124333251258 37.0 33.0 39.0 17.0 39.0 12 32.84385462054668 37.0 33.0 39.0 13.0 39.0 13 32.982769620879964 37.0 33.0 39.0 16.0 39.0 14 33.95527801092917 39.0 33.0 40.0 16.0 41.0 15 33.508670284329824 38.0 33.0 40.0 10.0 41.0 16 33.58574854747061 38.0 33.0 40.0 10.0 41.0 17 33.82777102885741 39.0 33.0 40.0 11.0 41.0 18 33.8834738816782 39.0 33.0 40.0 10.0 41.0 19 33.208118112303545 39.0 32.0 40.0 8.0 41.0 20 33.506949423001316 39.0 33.0 40.0 8.0 41.0 21 33.517360293947604 39.0 33.0 40.0 2.0 41.0 22 33.52573741968747 39.0 33.0 40.0 2.0 41.0 23 33.51742014999381 39.0 34.0 40.0 2.0 41.0 24 32.13339736116458 38.0 31.0 40.0 2.0 41.0 25 32.730141736396696 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.9791369162479704 1101 2 -1.1453714343912687 1101 3 -1.9535165896766884 1101 4 -1.3969649177045085 1101 5 -1.3646879674757315 1101 6 -3.2076096654023587 1101 7 -1.5493769912352242 1101 8 -1.1560479261209053 1101 9 -1.3868767133053126 1101 10 -10.1099212972041 1101 11 -5.0205163303330025 1101 12 -2.8360124987667454 1101 13 -2.077666916934593 1101 14 -1.7222541681544357 1101 15 -2.8852621999033055 1101 16 -1.661132219194858 1101 17 -1.46605832913734 1101 18 -1.2451148109707546 1101 19 -2.042740600129715 1101 20 -1.3141881693227049 1101 21 -1.297405570810298 1101 22 -1.0229664324511631 1101 23 -1.133982462423326 1101 24 -1.3787296646112956 1101 25 -1.3281811883443098 1102 1 -0.6459120599210522 1102 2 -0.5803524391241695 1102 3 -0.7428754669868383 1102 4 -0.6433303484407595 1102 5 -0.5086841189001987 1102 6 -1.202096203641684 1102 7 -0.3464165122384948 1102 8 0.15026048344110876 1102 9 -0.1665575550868823 1102 10 -0.1834073944608079 1102 11 -0.4758721467050151 1102 12 -0.44836755815790497 1102 13 -0.5503483977872179 1102 14 -0.6137913109172501 1102 15 -0.13472394171387947 1102 16 -0.19670914000428041 1102 17 -0.4308999880809168 1102 18 -0.6947260623945155 1102 19 -0.3194560107033979 1102 20 -0.5288877469698363 1102 21 -0.5680014633048671 1102 22 -0.5139663782126576 1102 23 -0.8861950396247664 1102 24 -4.078642175547429 1102 25 -2.40507487851983 1103 1 -0.5401080217727099 1103 2 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1.5157644208651355 1110 9 1.2157531050262165 1110 10 1.3449843395912993 1110 11 1.0314477623906981 1110 12 1.2058896164100332 1110 13 0.8789840899248418 1110 14 0.7411687863291974 1110 15 1.3145863484399172 1110 16 1.0731973131430124 1110 17 0.5844691784536238 1110 18 0.5211769025650526 1110 19 -1.8312207165857473 1110 20 0.013631147380358755 1110 21 0.20080568219484007 1110 22 0.41470018275135345 1110 23 0.5543366447470959 1110 24 -0.5702388142028916 1110 25 0.04418256030475476 1111 1 0.3372490470155718 1111 2 -0.1375846839499104 1111 3 0.5925673237471756 1111 4 0.24430083200516606 1111 5 0.16750957432770974 1111 6 0.5262893261239547 1111 7 0.4916332277559441 1111 8 1.0102596102452353 1111 9 0.21907791321036285 1111 10 0.2257905751289968 1111 11 0.1438579438545915 1111 12 0.4057191134262297 1111 13 0.2310240363381908 1111 14 0.20722651376799917 1111 15 -0.8557217748093819 1111 16 -0.42319776662802866 1111 17 0.018058266262656275 1111 18 0.20451632529066188 1111 19 0.8557966990761656 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1.0274085890932732 2215 2 0.41035101206762903 2215 3 0.5720338929835656 2215 4 0.9420721144311806 2215 5 1.40333955849529 2215 6 1.9323787184026067 2215 7 1.2426379774819196 2215 8 -0.23885742787436115 2215 9 0.3636522646900815 2215 10 0.1399423227845773 2215 11 0.16169986323103558 2215 12 0.02101566683020195 2215 13 0.5067151823618161 2215 14 0.6394881140602919 2215 15 0.5129056761710089 2215 16 0.6773989938152809 2215 17 0.22732632131076258 2215 18 -0.5107558705441946 2215 19 0.7343255019016439 2215 20 -2.95275540724149 2215 21 -1.641211124527846 2215 22 -0.334039313046965 2215 23 -0.2280162964293737 2215 24 -0.23494469655583217 2215 25 0.10888844265770103 2216 1 0.5033189532389315 2216 2 -0.5889487078203253 2216 3 0.3816977585297856 2216 4 0.20593766064967056 2216 5 0.4648241523328238 2216 6 -1.0925512535861905 2216 7 -9.129210762013876 2216 8 -10.558325214989207 2216 9 -5.25189395379731 2216 10 -2.8590772850585573 2216 11 -1.7748547586177033 2216 12 -1.3833260698644771 2216 13 -0.8490271145569466 2216 14 -1.1570104853794874 2216 15 -1.984153147358402 2216 16 -1.2559343395180491 2216 17 -1.4380798411542202 2216 18 -1.1419883635413939 2216 19 -0.3667949462776221 2216 20 -1.5253044268493348 2216 21 -4.023984233771543 2216 22 -2.562750797640806 2216 23 -2.26513114236775 2216 24 -2.50707354809645 2216 25 -1.5156213612638716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47598.0 3 1.0 4 378.0 5 2201.0 6 2595.0 7 2185.0 8 1680.0 9 1580.0 10 1483.0 11 1453.0 12 1448.0 13 1499.0 14 1430.0 15 1569.0 16 1543.0 17 1794.0 18 1942.0 19 2489.0 20 3217.0 21 4112.0 22 5095.0 23 6229.0 24 7084.0 25 8093.0 26 9010.0 27 10176.0 28 11505.0 29 13798.0 30 16252.0 31 19691.0 32 25602.0 33 33303.0 34 44770.0 35 61701.0 36 88834.0 37 137984.0 38 153773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08389929957844 18.55278921522528 12.61426657453104 25.74904491066524 2 14.148784912320842 19.932231828160834 42.33563222147772 23.583351038040608 3 18.843581886278514 24.148995573714675 29.951651344909774 27.05577119509704 4 11.496448242908057 16.690399501202073 38.99622362047794 32.81692863541193 5 12.867390925716277 37.95692545502046 36.42980384045981 12.745879778803449 6 31.82189903784006 37.54655338279977 18.503923615032612 12.127623964327556 7 27.732942039618493 31.109214039662124 23.041508590006192 18.11633533071319 8 24.27905822485175 37.685732245585854 20.84441784011312 17.190791689449277 9 25.725439291147232 15.811215701001721 20.3748908065214 38.08845420132964 10 13.7267950534219 29.812836225913873 35.455783868232785 21.004584852431442 11 34.201728808624175 22.942566707984277 24.475581564635753 18.38012291875579 12 22.698569280694475 25.14419446353128 32.20281789793794 19.9544183578363 13 28.552710529860526 22.396923943523994 25.830955675560947 23.219409851054536 14 19.965385259073106 22.675784784481056 28.18733867884351 29.17149127760233 15 22.77479502453175 31.493837810184417 23.265293904752056 22.46607326053178 16 21.27353278942814 29.0153374733989 28.388748891053865 21.322380846119096 17 21.062234927701677 29.303304619010234 28.20239992053784 21.432060532750246 18 20.82632056590142 28.548278907001517 29.81717596252359 20.80822456457347 19 23.26874404680909 27.586746496121922 27.518710028575317 21.62579942849367 20 22.696676912275546 28.216651767673675 28.432312990425185 20.654358329625595 21 22.43654808521755 27.460453952971974 27.521409153620713 22.581588808189768 22 21.960770440679443 28.80485362389041 27.844932213629114 21.389443721801033 23 21.584531045264736 28.499489076023583 27.926247341630578 21.989732537081107 24 22.772562823232317 27.56738811280629 28.391787841226517 21.268261222734875 25 21.97312528742826 28.0158537945973 28.281580372425392 21.729440545549046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 250.0 1 250.0 2 323.0 3 396.0 4 396.0 5 396.0 6 553.0 7 710.0 8 710.0 9 710.0 10 1074.5 11 1439.0 12 1439.0 13 1439.0 14 2564.0 15 3689.0 16 3689.0 17 3689.0 18 6445.5 19 9202.0 20 9202.0 21 9202.0 22 14397.0 23 19592.0 24 19592.0 25 19592.0 26 27651.5 27 35711.0 28 35711.0 29 35711.0 30 46476.0 31 57241.0 32 57241.0 33 57241.0 34 65642.0 35 74043.0 36 74043.0 37 74043.0 38 80871.0 39 87699.0 40 87699.0 41 87699.0 42 91161.5 43 94624.0 44 94624.0 45 94624.0 46 96428.5 47 98233.0 48 98233.0 49 98233.0 50 93262.0 51 88291.0 52 88291.0 53 88291.0 54 77410.0 55 66529.0 56 66529.0 57 66529.0 58 55975.0 59 45421.0 60 45421.0 61 45421.0 62 36436.5 63 27452.0 64 27452.0 65 27452.0 66 20953.5 67 14455.0 68 14455.0 69 14455.0 70 10340.0 71 6225.0 72 6225.0 73 6225.0 74 4378.0 75 2531.0 76 2531.0 77 2531.0 78 1725.0 79 919.0 80 919.0 81 919.0 82 605.5 83 292.0 84 292.0 85 292.0 86 199.0 87 106.0 88 106.0 89 106.0 90 69.0 91 32.0 92 32.0 93 32.0 94 21.5 95 11.0 96 11.0 97 11.0 98 7.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18990691024449835 2 0.11277423251625296 3 0.11522288895207027 4 0.07223536485661076 5 0.025166746701455724 6 0.02557485610742528 7 0.24527375298770093 8 0.23343858021458394 9 0.022173944391012345 10 0.03890643003576399 11 0.01904510561191244 12 0.02067754323579065 13 0.017684740925347265 14 0.020405470298477616 15 0.017820777394003784 16 0.022446017328325376 17 0.02122168911041672 18 0.017412667988034234 19 0.026799184325333937 20 0.02013339736116458 21 0.017820777394003784 22 0.01918114208056896 23 0.020949616173103685 24 0.018773032674599406 25 0.017820777394003784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 735097.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.59449676986132 #Duplication Level Percentage of deduplicated Percentage of total 1 93.76355547031581 82.1317145677529 2 4.805225812659636 8.418226738509373 3 0.756079275451568 1.9868515095390438 4 0.24027948359980436 0.8418864180018804 5 0.11300868655346401 0.4949469514636836 6 0.0674711218225204 0.3546059377525009 7 0.05517168193657745 0.3382915000626945 8 0.033117919280889786 0.23207579787795427 9 0.023678627940411395 0.18667057487770025 >10 0.12191137512098803 1.9656027375318046 >50 0.009940099370454302 0.5862164007998841 >100 0.009162739875420907 1.514203860404815 >500 0.0012424053975958007 0.7898183070694855 >1k 1.553006746994751E-4 0.1588886983563829 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1168 0.15889059539081238 No Hit CTGTAGGACGTGGAATATGGCAAGA 932 0.12678598878787425 No Hit GTCCTAAAGTGTGTATTTCTCATTT 829 0.11277423251625296 No Hit GTCCTACAGTGTGCATTTCTCATTT 823 0.11195801370431385 No Hit GTATCAACGCAGAGTACTTTTTTTT 793 0.10787691964461833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTAG 115 5.0367817E-9 12.434431 1 TAGTACC 70 1.0695202E-4 12.238385 4 TAAGACA 150 1.2732926E-11 12.0570755 4 GTATAAT 120 9.473297E-9 11.91633 1 TAGGACC 300 0.0 11.739784 4 GTCCTAC 650 0.0 11.733003 1 GTCCTAG 130 2.4647306E-9 11.733002 1 GGCGAGG 180 0.0 11.593645 19 GGGCTAT 60 0.0057207295 11.121908 1 GCCTAGG 60 0.0057207295 11.121908 1 CCTCTAT 60 0.0057207295 11.121908 1 TCCAACG 95 1.38021605E-5 10.983454 18 TGTAGGA 655 0.0 10.908262 2 TCAATAC 70 0.0014554829 10.8874855 3 TGTAGTG 115 8.623083E-7 10.755178 5 GTAGGAC 630 0.0 10.736271 3 GCACCAT 80 3.7139474E-4 10.703471 6 ACTGATC 80 3.7398006E-4 10.695441 8 GTCTAGA 100 2.3035613E-5 10.48637 1 CTGTAGG 660 0.0 10.399707 1 >>END_MODULE