FastQCFastQC Report
Thu 26 May 2016
SRR1926810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735097
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14290.1943961137101634No Hit
CTGTAGGACGTGGAATATGGCAAGA11590.15766626717290372No Hit
GTCCTACAGTGGACATTTCTAAATT11380.15480950133111684No Hit
GGTATCAACGCAGAGTACTTTTTTT9640.13113915578488283No Hit
GTCCTACAGTGTGCATTTCTCATTT9010.12256885825952223No Hit
CTTTAGGACGTGAAATATGGCGAGG8930.12148056651027007No Hit
GTCCTAAAGTGTGTATTTCTCATTT8580.11671929010729197No Hit
TATCAACGCAGAGTACTTTTTTTTT7690.1046120443968619No Hit
CTGTAGGACCTGGAATATGGCGAGA7620.1036597891162663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG400.005315514.23382418
TAGGACC3350.014.2132954
AAAGGCG707.071876E-613.6041545
GGCGAGG2000.013.28490419
TATGTCG500.001511112813.28490416
GTAGGAC7600.012.1542383
CTGTAGG7300.012.1410421
TGTAGGA7550.011.9881952
GTCCTAG1209.5187715E-911.91258051
AGAACAG2250.011.8507295
CCCTATA657.8227837E-411.7261062
CTAGTAC657.8542315E-411.7205013
TAAGACC657.8542315E-411.7205014
AGTACTG657.8542315E-411.7205015
TGCTAGG907.209943E-611.6446732
TAATAGT907.2484327E-611.6391094
CTATACT1156.818118E-811.5931044
AGGACGT5800.011.4931645
TGGCGAG6200.011.4788918
GGACGTG5750.011.4274886
Produced by FastQC (version 0.11.5)