##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926810_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 735097 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.45880475637909 31.0 30.0 33.0 25.0 34.0 2 29.803529330142826 31.0 30.0 33.0 26.0 34.0 3 29.875318495382242 31.0 30.0 34.0 26.0 34.0 4 33.50998439661704 35.0 35.0 37.0 28.0 37.0 5 33.456016008771634 35.0 35.0 37.0 28.0 37.0 6 33.42216605427583 37.0 35.0 37.0 28.0 37.0 7 33.3831956870998 37.0 35.0 37.0 28.0 37.0 8 33.432421843647845 37.0 35.0 37.0 28.0 37.0 9 34.934035916348456 39.0 35.0 39.0 28.0 39.0 10 34.79357554173123 39.0 35.0 39.0 28.0 39.0 11 34.85085505722374 39.0 35.0 39.0 28.0 39.0 12 34.73404190195308 39.0 35.0 39.0 27.0 39.0 13 34.75661443319725 39.0 35.0 39.0 27.0 39.0 14 35.79206145583508 39.0 36.0 41.0 27.0 41.0 15 35.78235253306706 39.0 36.0 41.0 27.0 41.0 16 35.75581181803218 39.0 36.0 41.0 27.0 41.0 17 35.730698125553495 39.0 36.0 41.0 27.0 41.0 18 35.694576362031135 39.0 36.0 41.0 27.0 41.0 19 35.76245311843199 40.0 36.0 41.0 27.0 41.0 20 35.730940270467705 40.0 36.0 41.0 27.0 41.0 21 35.678488689247814 40.0 36.0 41.0 27.0 41.0 22 35.59057512137854 40.0 36.0 41.0 27.0 41.0 23 35.510614245466925 40.0 36.0 41.0 27.0 41.0 24 35.430155476080024 40.0 36.0 41.0 27.0 41.0 25 35.30583854919827 39.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.16277259367802444 1101 2 -0.16533984340131624 1101 3 0.16625712055397202 1101 4 0.10557343059665669 1101 5 0.01761586466874121 1101 6 0.10556850673237506 1101 7 0.06789003566643004 1101 8 0.2298041429790132 1101 9 0.07565682670827556 1101 10 0.17781478378401516 1101 11 0.2239565672979964 1101 12 0.2802620634952575 1101 13 0.2651015508107477 1101 14 0.2050083921414938 1101 15 0.1945696282244853 1101 16 0.07403246111678641 1101 17 0.03792338872512602 1101 18 0.16117913621432933 1101 19 0.18793034198689185 1101 20 -0.013735103267158877 1101 21 0.09653260531306529 1101 22 0.14805487226749392 1101 23 0.2663967419169424 1101 24 0.26670912422665793 1101 25 0.24723615667075904 1102 1 -0.2361360647535733 1102 2 -0.21364040960412467 1102 3 -0.008850707948596437 1102 4 -0.18703857780794664 1102 5 -0.10829528323022686 1102 6 -0.17342020458437446 1102 7 -0.09289646979397048 1102 8 0.16963241916147354 1102 9 0.10753020102705335 1102 10 -0.002962147401476045 1102 11 0.057713196775914355 1102 12 0.2647050458583067 1102 13 0.26427382408790834 1102 14 0.21851142124398848 1102 15 0.11054239577948977 1102 16 0.24631752925685646 1102 17 0.15386880120893665 1102 18 -0.04491302679359421 1102 19 0.0050027362394970964 1102 20 0.011846604679725203 1102 21 0.14488033543170786 1102 22 0.19515040042081466 1102 23 0.11960848667526136 1102 24 0.10890809463837314 1102 25 -0.0330542787229291 1103 1 -0.5641188633890337 1103 2 -0.5368013262832392 1103 3 -0.39660919661812244 1103 4 -0.6345632739511586 1103 5 -0.7532089563711111 1103 6 -0.7976002594409835 1103 7 -0.6175902423595048 1103 8 -0.5759955278404973 1103 9 -0.5867046393221855 1103 10 -0.6264428634296095 1103 11 -0.6430669808798513 1103 12 -0.6679023974774694 1103 13 -0.5677781782732509 1103 14 -0.6614772945091545 1103 15 -0.643868772780138 1103 16 -0.6297928192983591 1103 17 -0.5316373423048688 1103 18 -0.6471551781968969 1103 19 -0.5609875868076273 1103 20 -0.6081990538485371 1103 21 -0.5548638725018762 1103 22 -0.8437802512444463 1103 23 -0.764803843498008 1103 24 -0.6514320448026751 1103 25 -0.5670054008654191 1104 1 -0.06709794110906486 1104 2 -0.07001405930195048 1104 3 0.08282860595666364 1104 4 0.11340315791981936 1104 5 0.03208108582337843 1104 6 -0.12553704113098263 1104 7 -0.24970148209012422 1104 8 -0.07855906561636061 1104 9 -0.12709402836911465 1104 10 0.003525846100160379 1104 11 -0.17075896892605158 1104 12 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2214 10 -0.15289608323995907 2214 11 -0.13110346196305045 2214 12 -0.15797874463902417 2214 13 0.05155250914683762 2214 14 -0.043292923135275885 2214 15 -0.10174553826205113 2214 16 0.05536567681776461 2214 17 0.0517547480405014 2214 18 -0.041821224034755744 2214 19 -0.11621178730576531 2214 20 -0.11547753252232695 2214 21 -0.2607536162621784 2214 22 -0.2238390821646732 2214 23 -0.2415405012217846 2214 24 -0.35506315423853607 2214 25 -0.3217758050693149 2215 1 0.2464071412080564 2215 2 0.07030864171451512 2215 3 0.13250545715895612 2215 4 -0.05756867663993859 2215 5 0.28051609037581926 2215 6 0.21039143327831766 2215 7 0.09273325379357544 2215 8 0.25013027868264004 2215 9 -0.003484015980504296 2215 10 0.2958434125583551 2215 11 0.034442756524342144 2215 12 0.020172515865176877 2215 13 0.19440965200398352 2215 14 0.16006842140254207 2215 15 0.1923721087967678 2215 16 0.28225643312028836 2215 17 0.17444382367075661 2215 18 0.2993552465534819 2215 19 0.04933443118162728 2215 20 0.1803207868054031 2215 21 0.016557308107572055 2215 22 -0.21039370401341273 2215 23 0.13240907861015216 2215 24 0.14241583735810082 2215 25 0.08158553946849878 2216 1 -0.9351054638339598 2216 2 -0.7949574647280642 2216 3 -2.3058698929811 2216 4 -1.9385210575923146 2216 5 -1.1365707443580746 2216 6 -1.1802248132202848 2216 7 -1.0894796313604829 2216 8 -0.979981766135289 2216 9 -1.0382179095379271 2216 10 -0.9565375398225981 2216 11 -1.061355562803385 2216 12 -1.1974745429583464 2216 13 -1.2483074348307497 2216 14 -1.3400716346198678 2216 15 -1.110569067673822 2216 16 -1.2422533708012793 2216 17 -1.108749453640172 2216 18 -1.282997694622992 2216 19 -1.2127103867455418 2216 20 -1.2804635269200872 2216 21 -1.145207397774783 2216 22 -1.2305617712403034 2216 23 -1.223053106263798 2216 24 -1.4033824819696292 2216 25 -1.4579102588508306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21062.0 3 12.0 4 312.0 5 2165.0 6 2996.0 7 2160.0 8 1616.0 9 1370.0 10 1253.0 11 1112.0 12 1044.0 13 1080.0 14 985.0 15 981.0 16 1049.0 17 1083.0 18 1237.0 19 1460.0 20 1859.0 21 2479.0 22 3189.0 23 3799.0 24 4476.0 25 5352.0 26 6324.0 27 7105.0 28 8276.0 29 9928.0 30 12513.0 31 16306.0 32 21085.0 33 27725.0 34 37378.0 35 51753.0 36 76165.0 37 129904.0 38 266504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.42656546437877 19.701759236433915 9.987892225828375 28.88378307335895 2 14.196296180308293 21.13459263021305 39.995860741172415 24.673250448306238 3 18.84562951191704 24.585100911839216 29.075725376123472 27.493544200120272 4 12.021678771645103 17.65018766230851 38.06694898768462 32.26118457836177 5 12.678593292973266 38.760764573838514 35.54969049724509 13.010951635943133 6 31.507913323377405 38.5620602761268 17.620761109639865 12.309265290855928 7 28.206736558517985 32.03192075597494 21.38200817748267 18.37933450802441 8 24.719460362551903 38.74399329534756 19.58022454055172 16.956321801548818 9 25.960422576410004 16.049468378196362 19.75238600572777 38.23772303966585 10 13.992640106657234 30.310652799412296 34.41818070510771 21.27852638882276 11 34.210046581484946 23.16204479658693 24.22822206743432 18.399686554493808 12 22.851666387790388 25.496909914799897 31.786862342587945 19.86456135482177 13 28.886509752494238 22.374961569540766 25.604616552440913 23.133912125524084 14 20.310244770418056 22.899290841888895 27.628462638264068 29.16200174942899 15 22.435894819754555 31.636785605940027 23.134321251671587 22.79299832263383 16 21.48201454507859 29.22347346053702 27.779576489537394 21.514935504847 17 21.06922089087684 29.51043129060694 27.86969321013854 21.55065460837768 18 21.014132944084473 28.526264095193653 29.670068059600784 20.789534901121083 19 22.718349923070956 27.805597386997295 27.693638764074752 21.782413925857 20 22.79785768020558 28.186442222799624 28.263303454946392 20.7523966420484 21 22.22302342287431 27.45085767773956 27.473442465932347 22.852676433453787 22 22.175440792167564 28.76695184445046 27.569422810867138 21.488184552514838 23 21.528131096159132 28.521930528612664 27.890990812059407 22.058947563168793 24 22.286099075629682 27.682952543548794 28.604330052578238 21.42661832824329 25 21.958901034616456 28.12156913428247 28.10442755984246 21.815102271258613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 312.0 1 312.0 2 285.0 3 258.0 4 258.0 5 258.0 6 418.0 7 578.0 8 578.0 9 578.0 10 976.0 11 1374.0 12 1374.0 13 1374.0 14 2304.5 15 3235.0 16 3235.0 17 3235.0 18 5782.0 19 8329.0 20 8329.0 21 8329.0 22 13433.0 23 18537.0 24 18537.0 25 18537.0 26 26472.0 27 34407.0 28 34407.0 29 34407.0 30 45086.0 31 55765.0 32 55765.0 33 55765.0 34 64442.5 35 73120.0 36 73120.0 37 73120.0 38 79673.5 39 86227.0 40 86227.0 41 86227.0 42 90558.5 43 94890.0 44 94890.0 45 94890.0 46 97212.5 47 99535.0 48 99535.0 49 99535.0 50 95365.0 51 91195.0 52 91195.0 53 91195.0 54 79574.5 55 67954.0 56 67954.0 57 67954.0 58 57399.5 59 46845.0 60 46845.0 61 46845.0 62 37424.0 63 28003.0 64 28003.0 65 28003.0 66 21384.0 67 14765.0 68 14765.0 69 14765.0 70 10500.5 71 6236.0 72 6236.0 73 6236.0 74 4322.5 75 2409.0 76 2409.0 77 2409.0 78 1586.5 79 764.0 80 764.0 81 764.0 82 518.5 83 273.0 84 273.0 85 273.0 86 172.0 87 71.0 88 71.0 89 71.0 90 42.0 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11671929010729197 2 0.09060028812524061 3 0.013467610396995228 4 0.0 5 0.00639371402685632 6 0.3448524480442717 7 0.02067754323579065 8 0.011563099835803982 9 0.01115499042983443 10 0.0043531669970085585 11 0.005577495214917215 12 5.441458746260698E-4 13 4.081094059695523E-4 14 0.0 15 0.0021765834985042793 16 4.081094059695523E-4 17 1.3603646865651745E-4 18 8.162188119391046E-4 19 5.441458746260698E-4 20 8.162188119391046E-4 21 0.012107245710430052 22 0.0 23 4.081094059695523E-4 24 2.720729373130349E-4 25 0.005713531683573732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 735097.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.43414810765888 #Duplication Level Percentage of deduplicated Percentage of total 1 91.68021974774018 77.40941252723408 2 6.466244836288938 10.919437484152095 3 0.9942562065881264 2.518475274120629 4 0.32349561117897463 1.0925630538585271 5 0.14804457874579674 0.6250008944179286 6 0.08741442399276374 0.4428457453290423 7 0.059362777963435816 0.35085719106527663 8 0.03811032867060371 0.257425050832425 9 0.030742298736082602 0.23361298241870637 >10 0.14713031488871553 2.250740315139265 >50 0.01240936347554834 0.6981892447096042 >100 0.010474593120995997 1.6648741046763935 >500 0.0016114758536746603 1.02959319234849 >1k 4.8344275610239814E-4 0.5069729396974327 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1429 0.1943961137101634 No Hit CTGTAGGACGTGGAATATGGCAAGA 1159 0.15766626717290372 No Hit GTCCTACAGTGGACATTTCTAAATT 1138 0.15480950133111684 No Hit GGTATCAACGCAGAGTACTTTTTTT 964 0.13113915578488283 No Hit GTCCTACAGTGTGCATTTCTCATTT 901 0.12256885825952223 No Hit CTTTAGGACGTGAAATATGGCGAGG 893 0.12148056651027007 No Hit GTCCTAAAGTGTGTATTTCTCATTT 858 0.11671929010729197 No Hit TATCAACGCAGAGTACTTTTTTTTT 769 0.1046120443968619 No Hit CTGTAGGACCTGGAATATGGCGAGA 762 0.1036597891162663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.3603646865651743E-4 0.0 0.0 0.0 0.0 11 1.3603646865651743E-4 0.0 0.0 1.3603646865651743E-4 0.0 12 1.3603646865651743E-4 0.0 0.0 1.3603646865651743E-4 0.0 13 1.3603646865651743E-4 0.0 0.0 1.3603646865651743E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGAG 40 0.0053155 14.233824 18 TAGGACC 335 0.0 14.213295 4 AAAGGCG 70 7.071876E-6 13.604154 5 GGCGAGG 200 0.0 13.284904 19 TATGTCG 50 0.0015111128 13.284904 16 GTAGGAC 760 0.0 12.154238 3 CTGTAGG 730 0.0 12.141042 1 TGTAGGA 755 0.0 11.988195 2 GTCCTAG 120 9.5187715E-9 11.9125805 1 AGAACAG 225 0.0 11.850729 5 CCCTATA 65 7.8227837E-4 11.726106 2 CTAGTAC 65 7.8542315E-4 11.720501 3 TAAGACC 65 7.8542315E-4 11.720501 4 AGTACTG 65 7.8542315E-4 11.720501 5 TGCTAGG 90 7.209943E-6 11.644673 2 TAATAGT 90 7.2484327E-6 11.639109 4 CTATACT 115 6.818118E-8 11.593104 4 AGGACGT 580 0.0 11.493164 5 TGGCGAG 620 0.0 11.47889 18 GGACGTG 575 0.0 11.427488 6 >>END_MODULE