FastQCFastQC Report
Thu 26 May 2016
SRR1926809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742033
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10750.14487226309342038No Hit
GTCCTACAGTGTGCATTTCTCATTT9910.13355201183774845No Hit
GTATCAACGCAGAGTACTTTTTTTT9520.12829618089761505No Hit
GTCCTAAAGTGTGTATTTCTCATTT9170.1235794095410851No Hit
CTGTAGGACGTGGAATATGGCAAGA7940.10700332734527979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA706.939361E-613.6291161
TAAGGGT655.333232E-513.18294054
GTTATAA603.943813E-412.7205081
TAGGACC2150.012.3994584
GACGTGG2150.012.3935877
GGCGAGG2150.011.46876819
GTATAAG751.9909891E-411.4484571
GTCCTAC6600.011.2749961
GTAGAAC1701.0913936E-1111.2133323
TCTATAC855.13857E-511.2133323
GGACGTG4950.011.1506526
GTCCCAA600.005688223511.1304451
TCCTACA7100.011.00801852
AGGACGT4800.010.9043275
ATGGCGA5250.010.83861417
TGGCGAG4750.010.7815718
GCCATAT2050.010.7038431
TAAAGCT909.288256E-510.5789024
AGGCAGT909.332979E-510.57389456
TGTAGGA6400.010.5738222
Produced by FastQC (version 0.11.5)