##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926809_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 742033 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.134084333176556 31.0 27.0 31.0 16.0 31.0 2 27.825877016251297 31.0 28.0 31.0 16.0 34.0 3 27.635907028393618 31.0 28.0 31.0 15.0 34.0 4 31.32336028182035 35.0 32.0 37.0 17.0 37.0 5 31.0828911921707 35.0 32.0 37.0 16.0 37.0 6 30.77925105756752 35.0 31.0 37.0 11.0 37.0 7 30.852573942129258 35.0 32.0 37.0 11.0 37.0 8 30.503947937625416 35.0 30.0 37.0 10.0 37.0 9 32.653888708453664 37.0 32.0 39.0 16.0 39.0 10 32.78403925431888 37.0 33.0 39.0 16.0 39.0 11 32.97322895342929 37.0 33.0 39.0 17.0 39.0 12 32.821371825781334 37.0 33.0 39.0 11.0 39.0 13 32.951712390149765 37.0 33.0 39.0 15.0 39.0 14 33.929706630298114 39.0 33.0 40.0 11.0 41.0 15 33.474864325441054 38.0 33.0 40.0 10.0 41.0 16 33.55457371841953 38.0 33.0 40.0 10.0 41.0 17 33.78422522987522 39.0 33.0 40.0 10.0 41.0 18 33.82840790099632 39.0 33.0 40.0 10.0 41.0 19 33.173334609107684 39.0 32.0 40.0 8.0 41.0 20 33.44803128701823 39.0 33.0 40.0 2.0 41.0 21 33.47155045665085 39.0 33.0 40.0 2.0 41.0 22 33.498016934556816 39.0 33.0 40.0 2.0 41.0 23 33.48333025620154 39.0 34.0 40.0 2.0 41.0 24 32.12042860627492 38.0 31.0 40.0 2.0 41.0 25 32.703921523705816 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5465673055273115 1101 2 -0.6341023402326904 1101 3 -1.5568529417832053 1101 4 -0.7975314847917048 1101 5 -0.7803852661886843 1101 6 -2.9401752873812725 1101 7 -0.9437478372553123 1101 8 -0.5507312882334894 1101 9 -0.6478395256000233 1101 10 -9.537886291338506 1101 11 -4.4739344677193955 1101 12 -2.070747999923647 1101 13 -1.2549087919812152 1101 14 -1.1052475480476005 1101 15 -2.616798926779186 1101 16 -0.9137405558102145 1101 17 -0.7412907327676663 1101 18 -0.4942399247588227 1101 19 -1.470370391575436 1101 20 -0.5150409891232854 1101 21 -0.6606979235289288 1101 22 -0.32331211844189767 1101 23 -0.2803206583818252 1101 24 -0.6290368731905005 1101 25 -0.4599802127238277 1102 1 -0.9859399537387823 1102 2 -0.8460747979590479 1102 3 -1.1639727132010727 1102 4 -0.9013068372672315 1102 5 -0.9998178703699914 1102 6 -1.860852290207319 1102 7 -0.8771451860772714 1102 8 -0.38147589766094114 1102 9 -0.6313672474293028 1102 10 -0.6095820772457259 1102 11 -0.8615888465349499 1102 12 -0.7819803761473594 1102 13 -1.1850522803569383 1102 14 -1.348626629034996 1102 15 -0.5909960008029529 1102 16 -0.7521670238727296 1102 17 -1.0636758561926527 1102 18 -1.1752929014790467 1102 19 -1.0686997434576497 1102 20 -1.0299472298527519 1102 21 -1.1711636752425179 1102 22 -1.1122811568043502 1102 23 -1.7263634082373969 1102 24 -4.8713636019990325 1102 25 -3.436695749751319 1103 1 -0.6349586152574425 1103 2 -0.4142939099281584 1103 3 -0.22683468231304005 1103 4 -0.14409158630197894 1103 5 -0.8093095048616235 1103 6 0.03778027092523928 1103 7 0.003646319714231794 1103 8 0.21407590414086286 1103 9 -0.4106304391924951 1103 10 -0.24746013387378696 1103 11 -0.5194717294178375 1103 12 -0.6070286386956241 1103 13 -0.26062338717804323 1103 14 -0.39013242054078745 1103 15 -0.11384027364722016 1103 16 0.10443690148120055 1103 17 -0.2251140801308793 1103 18 -0.4765235964597423 1103 19 -1.9649513522092583 1103 20 -0.7523088772143964 1103 21 -0.553250226079065 1103 22 -0.34599254676573565 1103 23 -0.5445181701421618 1103 24 0.7678094996740654 1103 25 0.14612278306721294 1104 1 -0.8336602054087656 1104 2 -0.5218038483739136 1104 3 -1.705949519450769 1104 4 -0.6594464849939534 1104 5 -0.7792184558747053 1104 6 -2.0290862333066144 1104 7 -0.3653008462257503 1104 8 0.007758579185743741 1104 9 -0.21028099061593508 1104 10 -0.0759818702164381 1104 11 -0.32059381200311066 1104 12 0.003458024551363792 1104 13 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2215 2 0.3011330058712751 2215 3 0.7953211833444804 2215 4 0.9137549132249845 2215 5 1.1068210533219123 2215 6 1.58649124538125 2215 7 0.8519670387303151 2215 8 -0.05252177797834534 2215 9 0.5020824274110964 2215 10 0.3755590241205411 2215 11 0.25917065468812694 2215 12 -0.011723529897132323 2215 13 0.3158844179307536 2215 14 0.3634863117203295 2215 15 0.0918361691153109 2215 16 0.5992275826543292 2215 17 0.040839089216333946 2215 18 -0.4820049115023153 2215 19 0.6085704074880027 2215 20 -3.5354451118406978 2215 21 -1.7587315411216373 2215 22 -0.8116323291687664 2215 23 -0.5491007150806624 2215 24 -0.3248702543467452 2215 25 -0.13535782242001204 2216 1 -0.5524698344047891 2216 2 -1.5134914292500241 2216 3 -0.7650717179649966 2216 4 -1.1649264778502229 2216 5 -0.9433346856145555 2216 6 -2.590968794145816 2216 7 -10.3084267294007 2216 8 -11.66641057055373 2216 9 -6.792090798205212 2216 10 -4.17251778782542 2216 11 -3.166984818423071 2216 12 -2.738520162006516 2216 13 -2.4586148481603445 2216 14 -2.7786016302141867 2216 15 -3.7156976658187517 2216 16 -2.7184610666065616 2216 17 -3.074599869210907 2216 18 -2.9501289941161843 2216 19 -2.209089347721598 2216 20 -3.1656908268528916 2216 21 -5.584403616754706 2216 22 -4.143279694609166 2216 23 -3.8226984867714293 2216 24 -4.431271153529622 2216 25 -3.7317007023478936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48709.0 3 8.0 4 425.0 5 2158.0 6 2605.0 7 2201.0 8 1751.0 9 1720.0 10 1620.0 11 1475.0 12 1466.0 13 1497.0 14 1491.0 15 1452.0 16 1619.0 17 1844.0 18 2034.0 19 2470.0 20 3194.0 21 4080.0 22 5159.0 23 6192.0 24 7363.0 25 8214.0 26 9389.0 27 10319.0 28 11633.0 29 13879.0 30 16479.0 31 19665.0 32 25239.0 33 33200.0 34 44738.0 35 61554.0 36 89042.0 37 139472.0 38 156677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.421312090933895 18.32156951726485 12.42737092892278 25.829747462878476 2 14.470035636360693 19.943947958229487 41.67092836700189 23.915088038407927 3 18.721139547624638 24.235628135685623 29.952123022780697 27.091109293909042 4 11.666783543081545 16.666082284592274 38.732394366197184 32.934739806129 5 13.068233898579246 37.78718356563233 36.33610115116059 12.808481384627827 6 31.976529454106267 37.370765070780976 18.367737569857628 12.284967905255128 7 27.58259088138832 31.234467968373103 23.085176681716156 18.097764468522428 8 24.530698334929987 37.164854497229385 20.960046697086433 17.34440047075419 9 26.034123933121624 15.560249311213074 20.052326239681676 38.353300515983626 10 13.87107820403585 29.51013276674684 35.47641260151641 21.142376427700903 11 34.36176966530665 22.734373631054194 24.50535986510366 18.398496838535497 12 22.80337350501888 25.094386163076106 32.121218656447844 19.98102167545717 13 28.7052537653018 22.300938393812693 25.705278027135513 23.28852981374999 14 20.30683670017105 22.383820847480703 27.86965236226787 29.43969009008038 15 22.95494062622151 31.23519025218692 23.042821905621977 22.76704721596959 16 21.61441203429126 28.616892219766 28.04105785302205 21.727637892920686 17 21.477317913153655 28.920520026688774 27.888823739528362 21.71333832062921 18 21.26667313643667 28.098615469220068 29.484089757275793 21.150621637067466 19 23.511285330687688 27.267850017793403 27.42111591592887 21.79974873559004 20 23.12334291695141 27.851126378403297 28.022040973674027 21.00348973097126 21 22.65658275689169 27.2901761404379 27.327512306108037 22.72572879656238 22 22.415031776733905 28.48300635534678 27.57991359963337 21.522048268285943 23 21.812937227890927 28.11980212699996 27.851298710051353 22.215961935057756 24 23.09533726107052 27.268389533402615 28.107582797212032 21.528690408314834 25 22.224932943348925 27.690151096494187 28.02536695825639 22.0595490019005 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 316.0 1 316.0 2 375.0 3 434.0 4 434.0 5 434.0 6 640.5 7 847.0 8 847.0 9 847.0 10 1302.0 11 1757.0 12 1757.0 13 1757.0 14 2889.5 15 4022.0 16 4022.0 17 4022.0 18 6810.0 19 9598.0 20 9598.0 21 9598.0 22 14715.5 23 19833.0 24 19833.0 25 19833.0 26 27368.5 27 34904.0 28 34904.0 29 34904.0 30 45037.0 31 55170.0 32 55170.0 33 55170.0 34 63403.0 35 71636.0 36 71636.0 37 71636.0 38 77958.5 39 84281.0 40 84281.0 41 84281.0 42 88851.5 43 93422.0 44 93422.0 45 93422.0 46 95261.0 47 97100.0 48 97100.0 49 97100.0 50 93463.0 51 89826.0 52 89826.0 53 89826.0 54 79862.5 55 69899.0 56 69899.0 57 69899.0 58 59764.5 59 49630.0 60 49630.0 61 49630.0 62 40133.5 63 30637.0 64 30637.0 65 30637.0 66 23570.0 67 16503.0 68 16503.0 69 16503.0 70 12027.5 71 7552.0 72 7552.0 73 7552.0 74 5280.0 75 3008.0 76 3008.0 77 3008.0 78 2065.5 79 1123.0 80 1123.0 81 1123.0 82 743.0 83 363.0 84 363.0 85 363.0 86 242.0 87 121.0 88 121.0 89 121.0 90 75.5 91 30.0 92 30.0 93 30.0 94 20.0 95 10.0 96 10.0 97 10.0 98 10.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21926248563069298 2 0.12613994256320135 3 0.1303176543361279 4 0.06859533201353579 5 0.02331432699084812 6 0.02250573761544298 7 0.273572738678738 8 0.26198295763126433 9 0.020079969489227568 10 0.037868935748140584 11 0.0175194364671113 12 0.018058496050714724 13 0.017115141779408732 14 0.018867085426119864 15 0.016576082195805305 16 0.020619029072830992 17 0.019945204593326712 18 0.016306552404003593 19 0.02493150574165839 20 0.019136615217921576 21 0.01684561198760702 22 0.01859755563431815 23 0.019271380113822432 24 0.0175194364671113 25 0.016576082195805305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 742033.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.97705096442012 #Duplication Level Percentage of deduplicated Percentage of total 1 94.61790625906993 84.1882226735999 2 4.156768296148378 7.3971396906736 3 0.6354810345440318 1.6962968519264023 4 0.2127570703716383 0.7572198677399191 5 0.1052255390799333 0.4681329076736903 6 0.06244719084400253 0.3333820129387009 7 0.03658982593317453 0.22789583647847317 8 0.028501533353833233 0.20287859086305116 9 0.02124754223603022 0.17014892835635367 >10 0.10475187508420135 1.7528599279158872 >50 0.008480886252855025 0.5147393358287009 >100 0.008480077503471137 1.329593674501951 >500 0.0012114396253000107 0.8166464519074266 >1k 1.5142995316250134E-4 0.14484324959585607 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1075 0.14487226309342038 No Hit GTCCTACAGTGTGCATTTCTCATTT 991 0.13355201183774845 No Hit GTATCAACGCAGAGTACTTTTTTTT 952 0.12829618089761505 No Hit GTCCTAAAGTGTGTATTTCTCATTT 917 0.1235794095410851 No Hit CTGTAGGACGTGGAATATGGCAAGA 794 0.10700332734527979 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.3476489590085616E-4 0.0 12 0.0 0.0 0.0 1.3476489590085616E-4 0.0 13 0.0 0.0 0.0 1.3476489590085616E-4 1.3476489590085616E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAA 70 6.939361E-6 13.629116 1 TAAGGGT 65 5.333232E-5 13.1829405 4 GTTATAA 60 3.943813E-4 12.720508 1 TAGGACC 215 0.0 12.399458 4 GACGTGG 215 0.0 12.393587 7 GGCGAGG 215 0.0 11.468768 19 GTATAAG 75 1.9909891E-4 11.448457 1 GTCCTAC 660 0.0 11.274996 1 GTAGAAC 170 1.0913936E-11 11.213332 3 TCTATAC 85 5.13857E-5 11.213332 3 GGACGTG 495 0.0 11.150652 6 GTCCCAA 60 0.0056882235 11.130445 1 TCCTACA 710 0.0 11.0080185 2 AGGACGT 480 0.0 10.904327 5 ATGGCGA 525 0.0 10.838614 17 TGGCGAG 475 0.0 10.78157 18 GCCATAT 205 0.0 10.703843 1 TAAAGCT 90 9.288256E-5 10.578902 4 AGGCAGT 90 9.332979E-5 10.5738945 6 TGTAGGA 640 0.0 10.573822 2 >>END_MODULE