FastQCFastQC Report
Thu 26 May 2016
SRR1926809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742033
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17450.23516474334699403No Hit
GGTATCAACGCAGAGTACTTTTTTT11040.1487804450745452No Hit
GTCCTACAGTGGACATTTCTAAATT10630.14325508434261008No Hit
CTGTAGGACGTGGAATATGGCAAGA10030.13516919058855875No Hit
GTCCTACAGTGTGCATTTCTCATTT9690.1305871841279296No Hit
GTCCTAAAGTGTGTATTTCTCATTT8870.11953646266405941No Hit
TATCAACGCAGAGTACTTTTTTTTT8810.11872787328865428No Hit
CTTTAGGACGTGAAATATGGCGAGG8510.11468492641162858No Hit
CTGTAGGACCTGGAATATGGCGAGA7590.10228655598874983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG402.7789146E-416.60907618
GTAAGTC400.00520802914.2777343
AGCACCG400.005210365614.2767695
ATTAGAC551.9259397E-413.8450753
TAGGACC3350.012.501054
CCTAGAC550.003021511512.1144413
AATGGTA550.003087927612.07932917
GTATTAA802.7704786E-511.9126051
TGTAGGA6800.011.7708642
TAAGGGT657.88951E-411.7142724
ATAATAC752.0339518E-411.4221873
TTAGGAC2750.011.4221853
CTAGACA855.2132746E-511.1974654
TATGGAC600.005786376511.1049033
GTCCTAC5850.010.9147981
CTAGGAG700.001465832710.8782723
CTGTAGG7200.010.8537071
CTGAACC1158.581046E-710.7593034
TCCTACA6200.010.7583172
GTAGGAC6650.010.7351373
Produced by FastQC (version 0.11.5)