##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926809_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 742033 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.772600409954812 31.0 30.0 33.0 25.0 34.0 2 30.120734522588616 31.0 30.0 34.0 26.0 34.0 3 30.23299368087403 31.0 30.0 34.0 26.0 34.0 4 33.81343282576381 35.0 35.0 37.0 28.0 37.0 5 33.77937234597383 37.0 35.0 37.0 30.0 37.0 6 33.72084260403513 37.0 35.0 37.0 29.0 37.0 7 33.68503826649219 37.0 35.0 37.0 29.0 37.0 8 33.69181424545808 37.0 35.0 37.0 29.0 37.0 9 35.2210966358639 39.0 35.0 39.0 29.0 39.0 10 35.126347480502886 39.0 35.0 39.0 29.0 39.0 11 35.16000231795621 39.0 35.0 39.0 29.0 39.0 12 35.05990434387689 39.0 35.0 39.0 28.0 39.0 13 35.06780830502147 39.0 35.0 39.0 29.0 39.0 14 36.16801004807064 40.0 36.0 41.0 29.0 41.0 15 36.15260642046917 40.0 36.0 41.0 29.0 41.0 16 36.12319667723673 40.0 36.0 41.0 28.0 41.0 17 36.09313736720604 40.0 36.0 41.0 28.0 41.0 18 36.05610127851457 40.0 36.0 41.0 28.0 41.0 19 36.12142451885563 40.0 36.0 41.0 28.0 41.0 20 36.08010964471931 40.0 36.0 41.0 28.0 41.0 21 36.029788432589925 40.0 36.0 41.0 27.0 41.0 22 35.94112930287467 40.0 36.0 41.0 27.0 41.0 23 35.8749907349134 40.0 36.0 41.0 27.0 41.0 24 35.804500608463506 40.0 36.0 41.0 27.0 41.0 25 35.68748559700175 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3652595473581748 1101 2 0.43361385484865167 1101 3 0.61080688547327 1101 4 0.49854236858615053 1101 5 0.43028913833190785 1101 6 0.5570070859037983 1101 7 0.39499902607860804 1101 8 0.3959625965437965 1101 9 0.4423100558360815 1101 10 0.536738496053843 1101 11 0.6442564965198656 1101 12 0.5887691163041353 1101 13 0.42346703273296527 1101 14 0.297396584808574 1101 15 0.34391298169986584 1101 16 0.4853594462047752 1101 17 0.5625472873066784 1101 18 0.5268075723645964 1101 19 0.4973400370953911 1101 20 0.3960335351886215 1101 21 0.45684131478726187 1101 22 0.3284413690723085 1101 23 0.5807315801858337 1101 24 0.5002975859607659 1101 25 0.4888926889786944 1102 1 -0.4939984975151219 1102 2 -0.4129051077881627 1102 3 -0.28810016715995346 1102 4 -0.45617838336163885 1102 5 -0.46265042167870973 1102 6 -0.1540004554330352 1102 7 -0.12476770772625656 1102 8 -0.15066722218308115 1102 9 -0.38795521810336453 1102 10 -0.23846877360750085 1102 11 -0.244476777482042 1102 12 -0.14855815897807645 1102 13 -0.20996154315661641 1102 14 -0.26302466833364946 1102 15 -0.3433291998230814 1102 16 -0.39335930721068735 1102 17 -0.3773109418063143 1102 18 -0.3801747999557321 1102 19 -0.30353119809127094 1102 20 -0.4677508731365094 1102 21 -0.32472066313519576 1102 22 -0.3048138965919662 1102 23 -0.37594086348834566 1102 24 -0.1971355490806843 1102 25 -0.271934516042613 1103 1 -0.44274367126029546 1103 2 -0.4995766007096556 1103 3 -0.21976844257822847 1103 4 -0.32911918130243123 1103 5 -0.5834596237379088 1103 6 -0.32772149790407923 1103 7 -0.2651875918961366 1103 8 -0.30983710635296546 1103 9 -0.34255624770439397 1103 10 -0.2525131751519041 1103 11 -0.12925798726325155 1103 12 -0.2707830624529777 1103 13 -0.22104584174091002 1103 14 -0.0926256979346789 1103 15 -0.2566657506596286 1103 16 -0.16749862510000213 1103 17 -0.08705193279730139 1103 18 -0.2778903726713011 1103 19 -0.19918756874763943 1103 20 -0.2723760340116712 1103 21 -0.22388411229864857 1103 22 -0.027505338033407156 1103 23 -0.20272657777405811 1103 24 -0.3006587151038502 1103 25 -0.23252814538624733 1104 1 -0.3378385673875073 1104 2 -0.18474427190739817 1104 3 -0.04105114327071391 1104 4 -0.007773964580195525 1104 5 -0.16491480455350427 1104 6 -0.22242687107668502 1104 7 -0.40403889992384023 1104 8 -0.3150371576481632 1104 9 -0.4474357039752377 1104 10 -0.3205979404763468 1104 11 -0.5466150018823868 1104 12 -0.2307455528351312 1104 13 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0.2930332964487832 1107 11 0.32560431910507504 1107 12 0.2970202938292914 1107 13 0.19730613760503957 1107 14 0.3070300679861475 1107 15 0.19602357808391702 1107 16 0.37033485166419666 1107 17 0.20020064895828682 1107 18 0.21406315572800594 1107 19 0.35025305087370384 1107 20 0.4185340348351403 1107 21 0.4276649410576354 1107 22 0.4263780526722698 1107 23 0.38137411069409666 1107 24 0.37092045873436064 1107 25 0.2684292556464598 1108 1 0.5243198208031963 1108 2 0.2174945226114673 1108 3 0.4094974304376464 1108 4 0.47103344385018886 1108 5 0.6562915972633121 1108 6 0.3883880869555085 1108 7 0.3473505643920163 1108 8 0.4906818191659639 1108 9 0.3454474152992688 1108 10 0.459498424359694 1108 11 0.32840379539852904 1108 12 0.2930218826019626 1108 13 0.2691098359147617 1108 14 0.3772155719065893 1108 15 0.14759249109861372 1108 16 0.3551738413224612 1108 17 0.39580063130525645 1108 18 0.4201255003445681 1108 19 0.3256903311302608 1108 20 0.3740601686745322 1108 21 0.4108533724388366 1108 22 0.19989850812043386 1108 23 0.4214950339475507 1108 24 0.3792100272648895 1108 25 0.44670720259910013 1109 1 0.5056137729832244 1109 2 0.3068919044139129 1109 3 0.5473314670058578 1109 4 0.46474760591878095 1109 5 0.23203540338685968 1109 6 0.4487364586962883 1109 7 0.1996820584956609 1109 8 0.07758001239326973 1109 9 0.20938769442941663 1109 10 0.39404348548703894 1109 11 0.422053043478158 1109 12 0.37153535225467493 1109 13 0.20260545422051024 1109 14 0.23769377162529537 1109 15 0.15749187441571877 1109 16 0.2650624056414017 1109 17 0.20595967057822406 1109 18 0.18749540189168812 1109 19 0.10574009852179955 1109 20 0.04081338732648021 1109 21 0.2381713427947787 1109 22 0.21323833285266858 1109 23 0.20844732216566086 1109 24 0.259746649573664 1109 25 0.2352498835468495 1110 1 0.6990655674052313 1110 2 0.4658586003168601 1110 3 0.4511094445047483 1110 4 0.7193624269097114 1110 5 0.6532544481183749 1110 6 0.8007911197178217 1110 7 0.5124482079327777 1110 8 0.6174297884289004 1110 9 0.540955300627509 1110 10 0.4343238650534005 1110 11 0.5734580093150186 1110 12 0.6183689905838037 1110 13 0.6243882899956148 1110 14 0.6753811455212642 1110 15 0.5233706052000784 1110 16 0.5334904681420838 1110 17 0.5553079902138194 1110 18 0.5496759233081647 1110 19 0.4543838598237855 1110 20 0.5301421439181837 1110 21 0.6353545532274438 1110 22 0.5285094181684684 1110 23 0.4525450218837648 1110 24 0.6975769564102592 1110 25 0.581955387547886 1111 1 0.25663455774366994 1111 2 0.25667490115265323 1111 3 0.3033204376332357 1111 4 0.504982104291706 1111 5 0.43380052885130027 1111 6 0.4877289106024705 1111 7 0.3297023215965922 1111 8 0.0683107970472534 1111 9 0.19201962637946934 1111 10 0.17535387999647867 1111 11 0.1905575840451732 1111 12 0.39370765603794666 1111 13 0.281650001104218 1111 14 0.03864908279341961 1111 15 0.30452462540669245 1111 16 0.12170824141447412 1111 17 0.2470891521480283 1111 18 0.4079771029985295 1111 19 0.23535226064080206 1111 20 0.20691403567473543 1111 21 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1113 8 -0.011027582543441383 1113 9 -0.03957248422445048 1113 10 -0.172510448870554 1113 11 0.029663011161559893 1113 12 -0.09287881551247068 1113 13 -0.01747592777023499 1113 14 -0.04602446202046906 1113 15 0.031764214201636776 1113 16 -0.08508844092455092 1113 17 -0.08611363763191093 1113 18 -0.16935711203880288 1113 19 -0.07802859625279268 1113 20 0.016012279328933232 1113 21 0.04530890842109159 1113 22 0.20302371200899927 1113 23 0.04941961149044971 1113 24 0.33804084869112927 1113 25 0.12020495166937195 1114 1 0.2993080004722266 1114 2 -0.07500593201664785 1114 3 0.157393411525117 1114 4 0.6025373536519751 1114 5 0.4799104370523608 1114 6 0.6186044899378658 1114 7 0.5158648397148013 1114 8 0.4874121337686148 1114 9 0.06311244530472493 1114 10 0.34898378533441843 1114 11 0.4064107057458699 1114 12 0.3001249922582687 1114 13 0.36221403667853735 1114 14 0.12974512273400762 1114 15 0.20680689371727112 1114 16 0.19154772748358084 1114 17 0.2474427480537571 1114 18 0.2548299920703414 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0.3744930340693955 2214 6 0.42877767153113666 2214 7 0.5251155642500436 2214 8 0.5640001029493646 2214 9 0.3706497323620468 2214 10 0.2739403132201872 2214 11 0.21135663067694566 2214 12 0.25378089103539025 2214 13 0.3821518326776925 2214 14 0.45704207615169423 2214 15 0.3696216247556521 2214 16 0.2120071888534838 2214 17 0.28136584128907316 2214 18 0.14511627184696607 2214 19 0.4103639262689711 2214 20 0.22237064372919235 2214 21 0.1649863195950374 2214 22 0.3901233995081199 2214 23 0.38900930262460776 2214 24 0.3868412848961498 2214 25 0.38227667188949965 2215 1 0.1256811158730997 2215 2 0.09677510222317309 2215 3 0.1342582840064459 2215 4 0.10739926713654313 2215 5 0.1661560337833734 2215 6 0.0748388143002785 2215 7 -0.23540400627646108 2215 8 -0.22819093795182965 2215 9 -0.30923079831934075 2215 10 -0.09074638234206134 2215 11 -0.29394578291131523 2215 12 -0.25546257995534205 2215 13 -0.3932904018165999 2215 14 -0.3496975995430063 2215 15 -0.22063216503995164 2215 16 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scores pass #Quality Count 2 18580.0 3 8.0 4 284.0 5 2063.0 6 2769.0 7 2005.0 8 1615.0 9 1376.0 10 1186.0 11 1048.0 12 1041.0 13 988.0 14 960.0 15 980.0 16 1037.0 17 1063.0 18 1176.0 19 1423.0 20 1781.0 21 2179.0 22 2852.0 23 3530.0 24 4227.0 25 4992.0 26 5941.0 27 6707.0 28 7440.0 29 9374.0 30 11813.0 31 15278.0 32 19845.0 33 26356.0 34 35454.0 35 49294.0 36 72870.0 37 126775.0 38 295723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94269956395976 19.289518844709235 10.24829469412425 28.519486897206754 2 14.339273403783134 20.875104723590432 39.66491149254557 25.12071038008086 3 18.34865847708237 24.674559722233454 29.36443110963745 27.61235069104672 4 12.090836930433014 17.534799665243998 37.87473063866432 32.49963276565867 5 12.760276981878658 38.53447044331656 35.68864067019049 13.016611904614297 6 31.606196598820663 38.401118492306985 17.59678298247488 12.395901926397473 7 28.069771046902698 31.95247049625601 21.75573393722493 18.22202451961637 8 24.989622138001305 38.141203616347966 19.63410623696028 17.235068008690448 9 26.055682171974 15.713763930988286 19.702383278905494 38.528170618132215 10 14.058646426039681 29.90191453977461 34.571418559957735 21.468020474227973 11 34.335036381254525 22.881174334874657 24.196263357425778 18.587525926445043 12 22.91524601431209 25.268385375254372 31.756263223858873 20.060105386574666 13 28.832547646578423 22.29005452639126 25.480239236900054 23.397158590130264 14 20.407637955826644 22.558895787372766 27.6365812210002 29.39688503580039 15 22.649860042128868 31.285873891017495 23.234084761775126 22.830181305078508 16 21.78815595658918 28.797065335897482 27.624736027125696 21.790042680387643 17 21.272802251115856 29.17690881255795 27.688159001229057 21.862129935097137 18 21.200653882799728 28.038526796122525 29.50450723624009 21.256312084837656 19 22.983002057865505 27.430525139785267 27.590895798153987 21.995577004195244 20 23.062679406493 27.888360308882394 28.130264275029308 20.9186960095953 21 22.453685920115372 27.16333420940905 27.42898732385824 22.953992546617336 22 22.60209451601209 28.32663776408866 27.430585971243865 21.640681748655382 23 21.79838605343166 28.182881600793497 27.74435603855359 22.274376307221253 24 22.521939754619748 27.330222955344247 28.530710265864546 21.617127024171463 25 22.25753238561933 27.728181823082604 28.00554180885072 22.008743982447346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 263.0 2 274.5 3 286.0 4 286.0 5 286.0 6 488.0 7 690.0 8 690.0 9 690.0 10 1132.5 11 1575.0 12 1575.0 13 1575.0 14 2692.5 15 3810.0 16 3810.0 17 3810.0 18 6437.0 19 9064.0 20 9064.0 21 9064.0 22 13970.0 23 18876.0 24 18876.0 25 18876.0 26 26510.0 27 34144.0 28 34144.0 29 34144.0 30 44414.5 31 54685.0 32 54685.0 33 54685.0 34 62712.5 35 70740.0 36 70740.0 37 70740.0 38 77588.5 39 84437.0 40 84437.0 41 84437.0 42 88906.0 43 93375.0 44 93375.0 45 93375.0 46 95712.5 47 98050.0 48 98050.0 49 98050.0 50 94727.5 51 91405.0 52 91405.0 53 91405.0 54 81039.5 55 70674.0 56 70674.0 57 70674.0 58 60480.0 59 50286.0 60 50286.0 61 50286.0 62 40507.5 63 30729.0 64 30729.0 65 30729.0 66 23798.5 67 16868.0 68 16868.0 69 16868.0 70 12239.5 71 7611.0 72 7611.0 73 7611.0 74 5287.5 75 2964.0 76 2964.0 77 2964.0 78 2002.5 79 1041.0 80 1041.0 81 1041.0 82 689.0 83 337.0 84 337.0 85 337.0 86 220.0 87 103.0 88 103.0 89 103.0 90 60.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11010291995099948 2 0.1072728571370815 3 0.015497963028598459 4 0.0 5 0.004716771356529966 6 0.3323302332915113 7 0.0239881514703524 8 0.009298777817159074 9 0.009703072504861644 10 0.0036386521893231167 11 0.004042946877025684 12 4.042946877025685E-4 13 9.433542713059932E-4 14 2.695297918017123E-4 15 0.003234357501620548 16 8.08589375405137E-4 17 1.3476489590085616E-4 18 5.390595836034246E-4 19 2.695297918017123E-4 20 4.042946877025685E-4 21 0.011859310839275343 22 0.0 23 1.3476489590085616E-4 24 1.3476489590085616E-4 25 0.004986301148331678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 742033.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.86098714884332 #Duplication Level Percentage of deduplicated Percentage of total 1 92.58548469932967 79.49481111938573 2 5.774050570980826 9.915313637435123 3 0.8783573779363393 2.2624989461725122 4 0.28671852837342465 0.9847174352002352 5 0.1401283036871218 0.6015777241034588 6 0.0803936274578579 0.414160572840482 7 0.04776260166830123 0.2870660889626134 8 0.03327476204011918 0.22856031327259935 9 0.025197054334254898 0.19470995625439497 >10 0.1252356974871655 1.9358865430450387 >50 0.011463402550586595 0.6726546603464157 >100 0.010049157203996486 1.533411673056054 >500 0.0012561446336026689 0.8120044340958362 >1k 6.280723168013344E-4 0.6626268958294927 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1745 0.23516474334699403 No Hit GGTATCAACGCAGAGTACTTTTTTT 1104 0.1487804450745452 No Hit GTCCTACAGTGGACATTTCTAAATT 1063 0.14325508434261008 No Hit CTGTAGGACGTGGAATATGGCAAGA 1003 0.13516919058855875 No Hit GTCCTACAGTGTGCATTTCTCATTT 969 0.1305871841279296 No Hit GTCCTAAAGTGTGTATTTCTCATTT 887 0.11953646266405941 No Hit TATCAACGCAGAGTACTTTTTTTTT 881 0.11872787328865428 No Hit CTTTAGGACGTGAAATATGGCGAGG 851 0.11468492641162858 No Hit CTGTAGGACCTGGAATATGGCGAGA 759 0.10228655598874983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 40 2.7789146E-4 16.609076 18 GTAAGTC 40 0.005208029 14.277734 3 AGCACCG 40 0.0052103656 14.276769 5 ATTAGAC 55 1.9259397E-4 13.845075 3 TAGGACC 335 0.0 12.50105 4 CCTAGAC 55 0.0030215115 12.114441 3 AATGGTA 55 0.0030879276 12.079329 17 GTATTAA 80 2.7704786E-5 11.912605 1 TGTAGGA 680 0.0 11.770864 2 TAAGGGT 65 7.88951E-4 11.714272 4 ATAATAC 75 2.0339518E-4 11.422187 3 TTAGGAC 275 0.0 11.422185 3 CTAGACA 85 5.2132746E-5 11.197465 4 TATGGAC 60 0.0057863765 11.104903 3 GTCCTAC 585 0.0 10.914798 1 CTAGGAG 70 0.0014658327 10.878272 3 CTGTAGG 720 0.0 10.853707 1 CTGAACC 115 8.581046E-7 10.759303 4 TCCTACA 620 0.0 10.758317 2 GTAGGAC 665 0.0 10.735137 3 >>END_MODULE