FastQCFastQC Report
Thu 26 May 2016
SRR1926808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816111
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12560.15390063361479014No Hit
CTGTAGGACGTGGAATATGGCAAGA10430.12780124272310997No Hit
GTCCTAAAGTGTGTATTTCTCATTT9480.1161606693207174No Hit
GTATCAACGCAGAGTACTTTTTTTT8930.10942138998249014No Hit
GTCCTACAGTGTGCATTTCTCATTT8470.10378490180870004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTAG350.002113342516.349211
TCCAACG902.3410394E-914.7508518
TAGGACC2850.014.0312374
AGTACTG905.2622636E-712.6925855
CCAACGA751.5044918E-512.64358519
CGGTTTC604.1517083E-412.64358413
GTCCTAG1301.8553692E-1012.4715141
TGCCGGT550.003105615312.07035510
GGCGAGG1650.012.06887619
GACTGCT951.0124295E-612.0245557
TGGCGAG5350.011.6982718
GCCGGTT658.139837E-411.67100211
GTAGGAC7050.011.3548833
TGTAGGA7050.011.2203972
AGGACGT5450.011.1787915
GAACTGA1201.236258E-711.1060126
CACTGTT1900.011.0225097
GGACGTG5300.010.9563356
GTCCTAA3050.010.9441431
ATGGCGA5900.010.92920117
Produced by FastQC (version 0.11.5)