FastQCFastQC Report
Thu 26 May 2016
SRR1926808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816111
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17610.21577947117487695No Hit
GTCCTACAGTGGACATTTCTAAATT13110.1606399129530174No Hit
CTGTAGGACGTGGAATATGGCAAGA12790.1567188777016852No Hit
GGTATCAACGCAGAGTACTTTTTTT10990.13466305441294138No Hit
GTCCTACAGTGTGCATTTCTCATTT9630.1179986545947794No Hit
TATCAACGCAGAGTACTTTTTTTTT9170.1123621664209893No Hit
GTCCTAAAGTGTGTATTTCTCATTT8950.1096664546856984No Hit
CTTTAGGACGTGAAATATGGCGAGG8770.10746087235682401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT250.00593946319.0496436
TAGGACC2700.014.816394
AATCCCG957.4920536E-812.98364919
GGCGAGG2200.012.93825119
GTAGGAC6700.012.2273953
ACTGTTC2450.012.0030828
TGTAGGA7450.011.8995622
TCCAACG802.8995568E-511.86006418
TGCCGGT802.905017E-511.85788310
GTCCTAC7600.011.5421181
GTGCAAG752.0023323E-411.4417511
GTGTAAC752.0023323E-411.4417511
AGGACGT5750.011.4297865
GGTATCA4250.011.2174021
TTACACT1451.1605152E-911.1670334
TCCTACA7900.011.1010742
CTGTAGG7500.011.060361
CGGTTTC951.3797724E-510.98414513
CCGGTTT951.3797724E-510.98414512
TATACAC3400.010.9268743
Produced by FastQC (version 0.11.5)